1.1.1.47: glucose 1-dehydrogenase [NAD(P)+]

This is an abbreviated version, for detailed information about glucose 1-dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

D-glucose
+
NAD(P)+
=
D-glucono-1,5-lactone
+
NAD(P)H
+
H+

Synonyms

alkali-resistant glucose 1-dehydrogenase, beta-D-glucose:NAD(P)+ 1-oxido-reductase, beta-D-glucose:NAD(P)+ 1-oxidoreductase, BmGlcDH-III, BmGlcDH-IV, D-glucose dehydrogenase (NAD(P)), EC 1.1.5.2, GDH, gdh-2, general stress protein 74, GlcDH, GlcDH-I, GlcDH-II, GlcDH-IWG3, glucose 1-dehydrogenase, glucose 1-dehydrogenase 3, glucose 1-dehydrogenase III, glucose 1-dehydrogenase IV, glucose dehydrogenase, glucose1-dehydrogenase, GSP74, hexose phosphate dehydrogenase, hexose-6-phosphate dehydrogenase, LsGDH, More, NAD(P)-dependent glucose-1-dehydrogenase, NAD-GDH, nicotinamide adenine dinucleotide (NAD)-linked glucose dehydrogenase, SsGDH, SSO3204

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.47 glucose 1-dehydrogenase [NAD(P)+]

Purification

Purification on EC 1.1.1.47 - glucose 1-dehydrogenase [NAD(P)+]

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
80C, anion-exchange chromatography, hydrophobic chromatography, gel filtration with 1 mM NADP+
-
ammonium sulfate, acetic acid pH 5.5, Sephadex G200, G150, crystallization
-
ammonium sulfate, DEAE-Sepharose, Blue Fractogel
-
ammonium sulfate, hydroxyapatite, Sephacryl S-200
-
ammonium sulfate, Sephacryl S-300, cation column, affinity column
-
ammonium sulfate, Sepharose 6B, DEAE-Sepharose, hydroxyapatite chromatography
-
ammonium sulfate, SP-Sephadex, Phenyl-Sepharose, Affi-Gel Blue
-
calcium-phosphate gel, ammonium sulfate, DEAE-cellulose
-
DEAE-cellulose, partially purified enzyme, 10 mM NAD(P)+ protects from inactivation on column
-
DEAE-Sephadex A-50 gel filtration
-
DEAE-Sephadex, Blue-Dextran Sepharose, DEAE-Sephadex, crystallization
-
DEAE-Sepharose CL-6B column chromatography; DEAE-Sepharose CL-6B column chromatography; DEAE-Sepharose CL-6B column chromatography
DEAE-Sepharose, 2',5'-ADP-Sepharose, Sephacryl
expressed in Escherichia coli, introduced cystein residue at position 44, ammonium sulfate, phenyl-Sepharose, thiopropyl-Sepharose
-
gel filtration
-
gel filtration on Superdex 75 26/60, anion exchange chromatography, and affinity chromatography using Reactive Red-500 dye affinity medium and an NaCl gradient of 0 to 1.5 M
-
gel filtration, DEAE-cellulose, hydroxylapatite, omega-aminohexyl-agarose
-
high-pressure liquid chromatography
hydroxyapatite, QAE-Sephadex
-
immobilized metal ion affinity chromatography (Ni2+)
methanol extraction, Mono Q, Matrex Gel Red A, Mono P, gel filtration
-
native enzyme by gel filtration, recombinant enzyme from Escherichia coli by heat denaturation, and affinity gel chromatography
-
Ni-NTA agarose gel filtration
-
recombinant catalytic alpha-subunit from Escherichia coli
recombinant enzyme from Escherichia coli
recombinant enzyme from Escherichia coli by heat treament and 2 chromatographical steps
-
recombinant enzyme is solubilized in reverse micelles of hexadecyltrimethylammonium bromide, i.e. CTAB, in cyclohexane with n-butanol as cosurfactant
-
recombinant wild-type and mutant enzymes from Escherichia coli
-
Sephadex G-100 gel filtration
-
streptomycin treatment, ammonium sulfate, DEAE-cellulose, gel filtration, DE 52 cellulose, hydroxyl apatite, gel filtration
-
Triton X-100, acid treatment, ammonium sulfate, Sephacryl S-200, Sephacryl S-300, DEAE-cellulose, Sephacryl S-300
-
Triton X-114, acid treatment, ammonium sulfate, Sephacryl S-200, Sephacryl S-300, SE-cellulose, DEAE-cellulose, purified enzyme contains 1.7% lipid
-