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1.1.1.47: glucose 1-dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about glucose 1-dehydrogenase [NAD(P)+], go to the full flat file.

Word Map on EC 1.1.1.47

Reaction

D-glucose
+
NAD(P)+
=
D-glucono-1,5-lactone
 +
NAD(P)H
 +
H+

Synonyms

alkali-resistant glucose 1-dehydrogenase, beta-D-glucose:NAD(P)+ 1-oxido-reductase, beta-D-glucose:NAD(P)+ 1-oxidoreductase, BmGlcDH-III, BmGlcDH-IV, bzgdh, D-glucose dehydrogenase (NAD(P)), EC 1.1.5.2, GDH, gdh-2, GDHB, general stress protein 74, GlcDH, GlcDH-I, GlcDH-II, GlcDH-IWG3, glucose 1-dehydrogenase, glucose 1-dehydrogenase 3, glucose 1-dehydrogenase B, glucose 1-dehydrogenase III, glucose 1-dehydrogenase IV, glucose dehydrogenase, glucose1-dehydrogenase, GluDH, GSP74, hexose phosphate dehydrogenase, hexose-6-phosphate dehydrogenase, LsGDH, More, NAD(P)-dependent glucose 1-dehydrogenase, NAD(P)-dependent glucose dehydrogenase, NAD(P)-dependent glucose-1-dehydrogenase, NAD-GDH, nicotinamide adenine dinucleotide (NAD)-linked glucose dehydrogenase, SsGDH, SSO3204

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.47 glucose 1-dehydrogenase [NAD(P)+]

pH Range

pH Range on EC 1.1.1.47 - glucose 1-dehydrogenase [NAD(P)+]

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 11
-
50% of maximal activity at pH 4.5 and pH 11.0
5 - 8
-
when incubated for 6 h at 37°C, the enzyme is highly stable in acidic conditions (pH 5.0-6.0) but is completely inactivated at pH values above 8.0
5.5 - 10.5
-
the enzyme's activity is more than 60% of its maximum at pH values ranging from 5.5 to 10.5
6 - 9.5
-
pH 6.0: about 40% of maximal activity, pH 9.5: about 45% of maximal activity
7 - 10
7 - 8.5
-
pH 7.0: 70% of maximal activity with glucose and NAD+ and about 55% of the activity with glucose and NADP+, pH 8.5: about 55% of maximal activity with glucose and NAD+ and about 75% of the activity with glucose and NADP+
7.5 - 10.5
-
at pH 7.5 and 10.0 respectively 75% and 50% activity compared to optimal pH
8 - 11
-
about 45% of maximal activity at pH 8.0 and pH 11.0
8.5 - 12
-
pH 8.5: about 35% of maximal activity, pH 12.0: about 65% of maximal activity
additional information
-
sharp drop in activity at alkaline pH of wild-type and mutant Q252L activities, not mutant Q252L/E170K