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Literature summary for 1.1.1.47 extracted from

  • Inose, K.; Fujikawa, M.; Yamazaki, T.; Kojima, K.; Sode, K.
    Cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from Burkholderia cepacia in Escherichia coli (2003), Biochim. Biophys. Acta, 1645, 133-138.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of the catalytic alpha-subunit in Escherichia coli as active water-soluble protein Burkholderia cepacia

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by EDTA and thiol reagents such as fluorescein 5-maleimide Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
beta-D-glucose pH 7.0, 70°C Burkholderia cepacia

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme does not contain weakly associated metal ions Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, recombinant catalytic alpha-subunit, SDS-PAGE Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia Q8GQE7 FERM BP-7306 strain SM-4
-

Purification (Commentary)

Purification (Comment) Organism
recombinant catalytic alpha-subunit from Escherichia coli Burkholderia cepacia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2000
-
above, purified recombinant catalytic alpha-subunit, 40°C Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + NAD(P)+
-
Burkholderia cepacia D-glucono-1,5-lactone + NAD(P)H + H+
-
ir
D-galactose + NAD(P)+
-
Burkholderia cepacia D-galactono-1,5-lactone + NAD(P)H
-
?
additional information Cys residues are not involved in the catalytic site Burkholderia cepacia ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, recombinant catalytic alpha-subunit, SDS-PAGE Burkholderia cepacia

Synonyms

Synonyms Comment Organism
GDH
-
Burkholderia cepacia
glucose dehydrogenase
-
Burkholderia cepacia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Burkholderia cepacia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermostable enzyme Burkholderia cepacia
60
-
30 min, stable Burkholderia cepacia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Burkholderia cepacia

Cofactor

Cofactor Comment Organism Structure
FAD the catalytic alpha-subunit contains a FAD binding motif at the N-terminal region Burkholderia cepacia
NAD+
-
Burkholderia cepacia
NADH
-
Burkholderia cepacia
NADP+
-
Burkholderia cepacia
NADPH
-
Burkholderia cepacia