Protein Variants | Comment | Organism |
---|---|---|
A11L | slight decrease in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
A246V | slight decrease in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
E170K | decrease in melting temperature by 7.1 degrees | Bacillus licheniformis |
E170K | increase in melting temperature by 18.5 degrees | Bacillus thuringiensis |
E170K | increase in melting temperature by 25.9 degrees | Bacillus subtilis subsp. subtilis |
E170K/K252L | increase in melting temperature by 5.6 degrees | Bacillus licheniformis |
E170R | increase in half-life at 65°C | Bacillus subtilis subsp. subtilis |
F155Y | increase in melting temperature by 1.9 degrees | Bacillus licheniformis |
F155Y | increase in melting temperature by 4.5 degrees | Bacillus thuringiensis |
F155Y | slight increase in half-life at 25°C, almost 100% increase in specific activity | Bacillus subtilis subsp. subtilis |
G114E | slight increase in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
G28A | slight decrease in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
I12V | slight decrease in half-life at 25°C, 4fold decrease in specific activity | Bacillus subtilis subsp. subtilis |
I186V | slight increase in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
I75M | slight decrease in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
I90V | half-life at 25°C similar to wild-type, decrease in specific activity | Bacillus subtilis subsp. subtilis |
K107E | slight decrease in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
K204E | no catalytic activity | Bacillus subtilis subsp. subtilis |
K234D | no catalytic activity | Bacillus subtilis subsp. subtilis |
K252L | increase in melting temperature by 8.9 degrees | Bacillus licheniformis |
K252Q | decrease in melting temperature by 2.7 degrees | Bacillus licheniformis |
L252Q | decrease in melting temperature by 19.3 degrees | Bacillus thuringiensis |
M23I | slight increase in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
M89L | slight decrease in half-life at 25°C, slight increase in specific activity | Bacillus subtilis subsp. subtilis |
N46A | slight increase in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
P105S | slight increase in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
P45A | slight increase in half-life at 25°C, 40% increase in specific activity | Bacillus subtilis subsp. subtilis |
P45A/F155Y/E170K/V227A/Q252L | strong increase in half-life at 65°C, about 50% increase in specific activity | Bacillus subtilis subsp. subtilis |
P45A/F155Y/E170R/V227A/W230F/Q252L | strong increase in half-life at 65°C, about 40% increase in specific activity | Bacillus subtilis subsp. subtilis |
P45A/F155Y/V227A | strong increase in half-life at 25°C, 50% increase in specific activity | Bacillus subtilis subsp. subtilis |
P45A/N46E/F155Y/E170K/V227A/W230F/Q252L | strong increase in half-life at 65°C, about 50% increase in specific activity | Bacillus subtilis subsp. subtilis |
P45A/N46E/F155Y/V227A | strong increase in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
P45A/N46E/F155Y/V227A/W230F | increase in half-life at 65°C | Bacillus subtilis subsp. subtilis |
P45A/V227A | strong increase in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
Q252L | increase in melting temperature by 17.3 degrees | Bacillus subtilis subsp. subtilis |
Q252L/E170K | strong increase in half-life at 65°C, about 50% increase in specific activity | Bacillus subtilis subsp. subtilis |
Q252L/E170R | strong increase in half-life at 65°C, about 50% increase in specific activity | Bacillus subtilis subsp. subtilis |
Q31G | no catalytic activity | Bacillus subtilis subsp. subtilis |
Q43E | half-life at 25°C similar to wild-type, slight increase in specific activity | Bacillus subtilis subsp. subtilis |
V140I | slight decrease in half-life at 25°C, decrease in specific activity | Bacillus subtilis subsp. subtilis |
V227A | slight increase in half-life at 25°C | Bacillus subtilis subsp. subtilis |
W230F | no catalytic acitivity | Bacillus subtilis subsp. subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Bacillus subtilis subsp. subtilis | - |
- |
- |
Bacillus subtilis subsp. subtilis 168 | - |
- |
- |
Bacillus thuringiensis | - |
- |
- |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50.2 | - |
wild-type, melting temperature | Bacillus subtilis subsp. subtilis |
52.9 | - |
mutant L252Q, melting temperature | Bacillus thuringiensis |
63.2 | - |
mutant E170K, melting temperature | Bacillus licheniformis |
65 | - |
half-life of wild-type, 8.7 min, of mutant K252L, 7.1 min, of mutant E170L/K252L, 4.2 min | Bacillus licheniformis |
65 | - |
half-life of wild-type, below 0.05 min, of mutant E170K 8.7 min, of mutant Q252L, 0.5 min, of mutant E170K/Q252L, 5323 min | Bacillus subtilis subsp. subtilis |
65 | - |
mutant E170K, half-life 3950 min | Bacillus thuringiensis |
67.5 | - |
mutant Q252L, melting temperature | Bacillus subtilis subsp. subtilis |
67.6 | - |
mutant K252Q, melting temperature | Bacillus licheniformis |
70.3 | - |
wild-type, melting temperature | Bacillus licheniformis |
72.1 | - |
mutant F155Y, melting temperature | Bacillus licheniformis |
72.2 | - |
wild-type, melting temperature | Bacillus thuringiensis |
75.9 | - |
mutant E170L/K252L, melting temperature | Bacillus licheniformis |
76.1 | - |
mutant E170K, melting temperature | Bacillus subtilis subsp. subtilis |
76.7 | - |
mutant F155Y, melting temperature | Bacillus thuringiensis |
79.2 | - |
mutant K252L, melting temperature | Bacillus licheniformis |
84.9 | - |
mutant E170K/Q252L, melting temperature | Bacillus subtilis subsp. subtilis |
90.7 | - |
mutant E170K, melting temperature | Bacillus thuringiensis |