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Literature summary for 1.1.1.47 extracted from

  • Vazquez-Figueroa, E.; Chaparro-Riggers, J.; Bommarius, A.S.
    Development of a thermostable glucose dehydrogenase by a structure-guided consensus concept (2007), ChemBioChem, 8, 2295-2301.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A11L slight decrease in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
A246V slight decrease in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
E170K decrease in melting temperature by 7.1 degrees Bacillus licheniformis
E170K increase in melting temperature by 18.5 degrees Bacillus thuringiensis
E170K increase in melting temperature by 25.9 degrees Bacillus subtilis subsp. subtilis
E170K/K252L increase in melting temperature by 5.6 degrees Bacillus licheniformis
E170R increase in half-life at 65°C Bacillus subtilis subsp. subtilis
F155Y increase in melting temperature by 1.9 degrees Bacillus licheniformis
F155Y increase in melting temperature by 4.5 degrees Bacillus thuringiensis
F155Y slight increase in half-life at 25°C, almost 100% increase in specific activity Bacillus subtilis subsp. subtilis
G114E slight increase in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
G28A slight decrease in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
I12V slight decrease in half-life at 25°C, 4fold decrease in specific activity Bacillus subtilis subsp. subtilis
I186V slight increase in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
I75M slight decrease in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
I90V half-life at 25°C similar to wild-type, decrease in specific activity Bacillus subtilis subsp. subtilis
K107E slight decrease in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
K204E no catalytic activity Bacillus subtilis subsp. subtilis
K234D no catalytic activity Bacillus subtilis subsp. subtilis
K252L increase in melting temperature by 8.9 degrees Bacillus licheniformis
K252Q decrease in melting temperature by 2.7 degrees Bacillus licheniformis
L252Q decrease in melting temperature by 19.3 degrees Bacillus thuringiensis
M23I slight increase in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
M89L slight decrease in half-life at 25°C, slight increase in specific activity Bacillus subtilis subsp. subtilis
N46A slight increase in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
P105S slight increase in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
P45A slight increase in half-life at 25°C, 40% increase in specific activity Bacillus subtilis subsp. subtilis
P45A/F155Y/E170K/V227A/Q252L strong increase in half-life at 65°C, about 50% increase in specific activity Bacillus subtilis subsp. subtilis
P45A/F155Y/E170R/V227A/W230F/Q252L strong increase in half-life at 65°C, about 40% increase in specific activity Bacillus subtilis subsp. subtilis
P45A/F155Y/V227A strong increase in half-life at 25°C, 50% increase in specific activity Bacillus subtilis subsp. subtilis
P45A/N46E/F155Y/E170K/V227A/W230F/Q252L strong increase in half-life at 65°C, about 50% increase in specific activity Bacillus subtilis subsp. subtilis
P45A/N46E/F155Y/V227A strong increase in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
P45A/N46E/F155Y/V227A/W230F increase in half-life at 65°C Bacillus subtilis subsp. subtilis
P45A/V227A strong increase in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
Q252L increase in melting temperature by 17.3 degrees Bacillus subtilis subsp. subtilis
Q252L/E170K strong increase in half-life at 65°C, about 50% increase in specific activity Bacillus subtilis subsp. subtilis
Q252L/E170R strong increase in half-life at 65°C, about 50% increase in specific activity Bacillus subtilis subsp. subtilis
Q31G no catalytic activity Bacillus subtilis subsp. subtilis
Q43E half-life at 25°C similar to wild-type, slight increase in specific activity Bacillus subtilis subsp. subtilis
V140I slight decrease in half-life at 25°C, decrease in specific activity Bacillus subtilis subsp. subtilis
V227A slight increase in half-life at 25°C Bacillus subtilis subsp. subtilis
W230F no catalytic acitivity Bacillus subtilis subsp. subtilis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-
Bacillus subtilis subsp. subtilis
-
-
-
Bacillus subtilis subsp. subtilis 168
-
-
-
Bacillus thuringiensis
-
-
-

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50.2
-
wild-type, melting temperature Bacillus subtilis subsp. subtilis
52.9
-
mutant L252Q, melting temperature Bacillus thuringiensis
63.2
-
mutant E170K, melting temperature Bacillus licheniformis
65
-
half-life of wild-type, 8.7 min, of mutant K252L, 7.1 min, of mutant E170L/K252L, 4.2 min Bacillus licheniformis
65
-
half-life of wild-type, below 0.05 min, of mutant E170K 8.7 min, of mutant Q252L, 0.5 min, of mutant E170K/Q252L, 5323 min Bacillus subtilis subsp. subtilis
65
-
mutant E170K, half-life 3950 min Bacillus thuringiensis
67.5
-
mutant Q252L, melting temperature Bacillus subtilis subsp. subtilis
67.6
-
mutant K252Q, melting temperature Bacillus licheniformis
70.3
-
wild-type, melting temperature Bacillus licheniformis
72.1
-
mutant F155Y, melting temperature Bacillus licheniformis
72.2
-
wild-type, melting temperature Bacillus thuringiensis
75.9
-
mutant E170L/K252L, melting temperature Bacillus licheniformis
76.1
-
mutant E170K, melting temperature Bacillus subtilis subsp. subtilis
76.7
-
mutant F155Y, melting temperature Bacillus thuringiensis
79.2
-
mutant K252L, melting temperature Bacillus licheniformis
84.9
-
mutant E170K/Q252L, melting temperature Bacillus subtilis subsp. subtilis
90.7
-
mutant E170K, melting temperature Bacillus thuringiensis