1.1.1.47: glucose 1-dehydrogenase [NAD(P)+]

This is an abbreviated version, for detailed information about glucose 1-dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

D-glucose
+
NAD(P)+
=
D-glucono-1,5-lactone
+
NAD(P)H
+
H+

Synonyms

alkali-resistant glucose 1-dehydrogenase, beta-D-glucose:NAD(P)+ 1-oxido-reductase, beta-D-glucose:NAD(P)+ 1-oxidoreductase, BmGlcDH-III, BmGlcDH-IV, D-glucose dehydrogenase (NAD(P)), EC 1.1.5.2, GDH, gdh-2, general stress protein 74, GlcDH, GlcDH-I, GlcDH-II, GlcDH-IWG3, glucose 1-dehydrogenase, glucose 1-dehydrogenase 3, glucose 1-dehydrogenase III, glucose 1-dehydrogenase IV, glucose dehydrogenase, glucose1-dehydrogenase, GSP74, hexose phosphate dehydrogenase, hexose-6-phosphate dehydrogenase, LsGDH, More, NAD(P)-dependent glucose-1-dehydrogenase, NAD-GDH, nicotinamide adenine dinucleotide (NAD)-linked glucose dehydrogenase, SsGDH, SSO3204

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.47 glucose 1-dehydrogenase [NAD(P)+]

Storage Stability

Storage Stability on EC 1.1.1.47 - glucose 1-dehydrogenase [NAD(P)+]

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 20 mM phosphate buffer, pH 7.3, 1 mM 2-mercaptoethanol, 50% v/v glycerol, stable for at least 3 months
-
-20°C, 20% glycerol, 3 months
-
-20°C, 20% glycerol, 4 years, stable
-
-20°C, 25 mM sodium phosphate buffer (pH 6.5) with 20% glycerol (w/v), 1 year, no loss of activity
0°C, 67 mM KH2PO4, pH 6.5, 0.5 M NaCl, stable for a few months
-
30°C, 25 mM sodium phosphate buffer (pH 6.5), 1 week
4°C, 20 mM MgCl2, 20% ethylene glycol, several months, stable
-
4°C, 50 mM sodium phosphate pH 6.5, 2 M NaCl, stable
-
4°C, 89% loss of activity after 4 days without stabilzing agent, complete stabilization by 10 mM NAD+ or 10 mM NADP+, and to a lesser extent by 800 mM NaCl. Partial stabilization by 800 MM (NH4)2SO4, 800 mM Na2SO4 or 800 mM dipicolinic acid
-
5°C, crystalline preparation as suspension in ammonium sulfate, stable for 6 months
-
5°C, crystalline preparation, in ammonium sulfate suspension, stable for several months
-
room temperature, 100 mM phosphate buffer at pH 7.0, 1 week, 30% loss of activity
-
room temperature, 100 mM phosphate buffer at pH 7.0, 3 days, no loss of activity
-