1.1.1.47: glucose 1-dehydrogenase [NAD(P)+]

This is an abbreviated version, for detailed information about glucose 1-dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

D-glucose
+
NAD(P)+
=
D-glucono-1,5-lactone
+
NAD(P)H
+
H+

Synonyms

alkali-resistant glucose 1-dehydrogenase, beta-D-glucose:NAD(P)+ 1-oxido-reductase, beta-D-glucose:NAD(P)+ 1-oxidoreductase, BmGlcDH-III, BmGlcDH-IV, D-glucose dehydrogenase (NAD(P)), EC 1.1.5.2, GDH, gdh-2, general stress protein 74, GlcDH, GlcDH-I, GlcDH-II, GlcDH-IWG3, glucose 1-dehydrogenase, glucose 1-dehydrogenase 3, glucose 1-dehydrogenase III, glucose 1-dehydrogenase IV, glucose dehydrogenase, glucose1-dehydrogenase, GSP74, hexose phosphate dehydrogenase, hexose-6-phosphate dehydrogenase, LsGDH, More, NAD(P)-dependent glucose-1-dehydrogenase, NAD-GDH, nicotinamide adenine dinucleotide (NAD)-linked glucose dehydrogenase, SsGDH, SSO3204

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.47 glucose 1-dehydrogenase [NAD(P)+]

pH Stability

pH Stability on EC 1.1.1.47 - glucose 1-dehydrogenase [NAD(P)+]

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
-
30C, 20 min, mutant enzymes E96K and E95A, less than 20% loss of activity
639105
4 - 8
-
30C, 20 min, mutant enzyme Q252L is stable
639105
4
-
30 min, about 50% loss of activity
639125
5 - 10
-
20 min, in presence of 2 M NaCl, isoenzyme GlcDH-III is stable
639101
5 - 6.5
-
30 min, stable
639125
5
-
30C, 20 min, about 55% loss of activity of mutant enzyme Y253C, about 35% loss of activity of mutant enzyme E96G and wild-type enzyme
639105
5 - 10.5
-
stable at 37C for 30 min
656567
5.5 - 10
-
20 min, in presence of 2 M NaCl, isoenzyme GlcDH-V is stable
639101
6 - 7
-
30C, 20 min, wild-type and mutant enzyme Y253C are stable
639105
6 - 9
-
20 min, in presence of 2 M NaCl, isoenzyme GlcDH-IWG3 is stable
639101
6 - 6.5
-
optimal stability
639115
6 - 8
-
30C, 30 min, stable
639121
6.5 - 7
-
20 min, without NaCl, isoenzyme GlcDH-IWG3 and GlcDH-III are stable
639101
6.5 - 9
-
the enzyme is stable and active at pH 6.5, by shifting the pH to 9.0 the enzyme is completely and irreversibly dissociated into four inactive protomers
639108
6.5
-
stable at
639124
6.5 - 10
-
711501
6.5 - 10.5
most stable at pH 8.5, retains more than 80% activity at pH 6.5-10.5 after 1 h at 25C, more than 30% activity after 24 h at pH 10.0
711501
7 - 9
wild type GlcDH shows reversible dissociation-association between inactive monomers and active tetramers when the pH is shifted between 9 and 7
677657
7.3
-
30 min, about 50% loss of activity
639125
7.5
-
30 min, about 95% loss of activity
639125
10
at pH 10 for 24 h enzyme retains 30% of its activity
711501
additional information