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Literature summary for 1.1.1.47 extracted from

  • Ding, H.T.; Du, Y.Q.; Liu, D.F.; Li, Z.L.; Chen, X.J.; Zhao, Y.H.
    Cloning and expression in E. coli of an organic solvent-tolerant and alkali-resistant glucose 1-dehydrogenase from Lysinibacillus sphaericus G10 (2010), Biores. Technol., 102, 1528-1536.
    View publication on PubMed
Search for more literature for 1.1.1.47

Activating Compound

Activating Compound Comment Organism Structure
EDTA 10-100 mM, sodium phosphate buffer, pH 8.0 Lysinibacillus sphaericus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Lysinibacillus sphaericus
His-tagged version expressed in Escherichia coli BL21(DE3) Lysinibacillus sphaericus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 60% inhibition at 50, 100 and 10 mM in sodium phosphate buffer at pH 8.0, Tris/HCl buffer at pH 8.0 and Tris/HCl buffer at pH 9.0, respectively Lysinibacillus sphaericus
Cd2+ more than 50% inhibition at 20 mM Lysinibacillus sphaericus
Co2+ more than 50% inhibition at 20 mM Lysinibacillus sphaericus
Cu2+ more than 50% inhibition at 20 mM Lysinibacillus sphaericus
Fe2+ complete inhibition at 10 mM Lysinibacillus sphaericus
Fe3+ complete inhibition at 10 mM Lysinibacillus sphaericus
Mn2+ more than 50% inhibition at 20 mM Lysinibacillus sphaericus
Zn2+ complete inhibition at 10 mM Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.072
-
 NADP+ pH 8.0, 25°C Lysinibacillus sphaericus
0.094
-
 NAD+ pH 8.0, 25°C Lysinibacillus sphaericus
5.1
-
 D-glucose with NADP+, pH 8.0, 25°C Lysinibacillus sphaericus
6
-
 D-glucose with NAD+, pH 8.0, 25°C Lysinibacillus sphaericus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ inhibits enzymatic activity Lysinibacillus sphaericus
Co2+ inhibits enzymatic activity Lysinibacillus sphaericus
Cu2+ inhibits enzymatic activity Lysinibacillus sphaericus
EDTA enhances activity Lysinibacillus sphaericus
Fe2+ inhibits enzymatic activity Lysinibacillus sphaericus
Fe3+ inhibits enzymatic activity Lysinibacillus sphaericus
K+ enhances activity Lysinibacillus sphaericus
K+ 50-200 mM, Tris/HCl buffer pH 9.0 Lysinibacillus sphaericus
Li+ enhances activity Lysinibacillus sphaericus
Li+ 50-200 mM, Tris/HCl buffer pH 9.0 Lysinibacillus sphaericus
Mg2+ enhances activity Lysinibacillus sphaericus
Mg2+ 1-200 mM, Tris/HCl buffer pH 8.0 Lysinibacillus sphaericus
Mn2+ inhibits enzymatic activity Lysinibacillus sphaericus
Na+ enhances activity Lysinibacillus sphaericus
Na+ 50-200 mM, Tris/HCl buffer pH 9.0 Lysinibacillus sphaericus
NH4+ enhances activity Lysinibacillus sphaericus
NH4+ 50-200 mM, Tris/HCl buffer pH 9.0 Lysinibacillus sphaericus
Zn2+ inhibits enzymatic activity Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27800
-
 calculated from cDNA Lysinibacillus sphaericus
28000
-
 SDS-PAGE Lysinibacillus sphaericus
120000
-
 gel filtration Lysinibacillus sphaericus

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 30%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
Acetone enzyme exhibited high tolerance to 30% acetone Lysinibacillus sphaericus
DMSO 60%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
DMSO enzyme exhibited high tolerance to 60% DMSO Lysinibacillus sphaericus
Ethanol 30%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
Ethanol enzyme exhibited high tolerance to 30% ethanol Lysinibacillus sphaericus
isopropanol 30%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
isopropanol enzyme exhibits high tolerance to 30% isopropanol Lysinibacillus sphaericus
Methanol 30%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
Methanol enzyme exhibited high tolerance to 30% methanol Lysinibacillus sphaericus
n-hexane 30%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
n-hexane enzyme exhibits high tolerance to 30% n-hexane Lysinibacillus sphaericus
n-hexanol 60%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
n-hexanol enzyme exhibited high tolerance to 60% n-hexanol Lysinibacillus sphaericus
n-propanol 10%: nearly 100% activity after 1h at 25°C Lysinibacillus sphaericus
n-propanol enzyme exhibits high tolerance to 10% n-propanol Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus C5IFU0
-
-
Lysinibacillus sphaericus G10 C5IFU0
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Lysinibacillus sphaericus

Storage Stability

Storage Stability Organism
-20°C, 25 mM sodium phosphate buffer (pH 6.5) with 20% glycerol (w/v), 1 year, no loss of activity Lysinibacillus sphaericus
30°C, 25 mM sodium phosphate buffer (pH 6.5), 1 week Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + NAD+ relative activity: 17.3% Lysinibacillus sphaericus D-galactono-1,5-lactone + NADH + H+
-
 ?
D-galactose + NAD+ relative activity: 17.3% Lysinibacillus sphaericus G10 D-galactono-1,5-lactone + NADH + H+
-
 ?
D-glucose + NAD+ relative activity: 100% Lysinibacillus sphaericus D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose + NAD+ about 20% activity with D-galactose, D-xylose, maltose Lysinibacillus sphaericus D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose + NAD+ relative activity: 100% Lysinibacillus sphaericus G10 D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose + NAD+ about 20% activity with D-galactose, D-xylose, maltose Lysinibacillus sphaericus G10 D-glucono-1,5-lactone + NADH + H+
-
 ?
D-glucose + NADP+
-
 Lysinibacillus sphaericus D-glucono-1,5-lactone + NADPH + H+
-
 ?
D-glucose + NADP+
-
 Lysinibacillus sphaericus G10 D-glucono-1,5-lactone + NADPH + H+
-
 ?
D-lactose + NAD+ relative activity: 4.2% Lysinibacillus sphaericus D-lactono-1,5-lactone + NADH + H+
-
 ?
D-maltose + NAD+ relative activity: 22.4% Lysinibacillus sphaericus ?
-
 ?
D-mannose + NAD+ relative activity: 7.6% Lysinibacillus sphaericus D-mannono-1,5-lactone + NADH + H+
-
 ?
D-xylose + NAD+ relative activity: 22.5% Lysinibacillus sphaericus D-xylono-1,5-lactone + NADH + H+
-
 ?
D-xylose + NAD+ relative activity: 22.5% Lysinibacillus sphaericus G10 D-xylono-1,5-lactone + NADH + H+
-
 ?
sucrose + NAD+ relative activity: 6.3% Lysinibacillus sphaericus ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 28000, SDS-PAGE, 120000 kDa by gel filtration, calculated: 261 amino acid residues, 27.8 kDa Lysinibacillus sphaericus
homotetramer gel filtration, 4 * 28000 Lysinibacillus sphaericus

Synonyms

Synonyms Comment Organism
alkali-resistant glucose 1-dehydrogenase
-
 Lysinibacillus sphaericus
GDH
-
 Lysinibacillus sphaericus
glucose 1-dehydrogenase
-
 Lysinibacillus sphaericus
LsGDH
-
 Lysinibacillus sphaericus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Lysinibacillus sphaericus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 35 stable for 1 week at 30°C (pH 6.5), completely inactivated in 30 min at 55°C, limited protection by glycerol, 84% activity after 30 min at 65°C in the presence of 2 M NaCl, Lysinibacillus sphaericus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
93
-
 NAD+ pH 8.0, 25°C Lysinibacillus sphaericus
93
-
 NADP+ pH 8.0, 25°C Lysinibacillus sphaericus
115
-
 NAD+ pH 8.0, 25°C Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Lysinibacillus sphaericus

pH Range

pH Minimum pH Maximum Comment Organism
4 10.5
-
 Lysinibacillus sphaericus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 10
-
 Lysinibacillus sphaericus
6.5 10.5 most stable at pH 8.5, retains more than 80% activity at pH 6.5-10.5 after 1 h at 25°C, more than 30% activity after 24 h at pH 10.0 Lysinibacillus sphaericus
10
-
 at pH 10 for 24 h enzyme retains 30% of its activity Lysinibacillus sphaericus