1.1.1.21: aldehyde reductase

This is an abbreviated version, for detailed information about aldehyde reductase, go to the full flat file.

Reaction

alditol
+
NAD(P)+
=
aldose
+
NAD(P)H
+
H+

Synonyms

17betaHSD5, 20-alpha-HSD, 20-alpha-hydroxysteroid dehydrogenase, AdhA, AKR1B, AKR1B1, AKR1B10, AKR1B13, AKR1B14, AKR1B3, AKR1C3, AKR4C7, aldehyde reductase, alditol/NADP+ oxidoreductase, alditol: NADP+ 1-oxidoreductase, alditol:NAD(P)+1oxidoreductase, alditol:NADP 1-oxidoreductase, alditol:NADP oxidoreductase, aldo-keto reductase, aldo-keto reductase family 1 member B7, aldoketo reductase 1C3, aldose reductase, aldose reductase 2, aldose reductase-like, aldose reductase-like protein, aldose xylose reductase, ALDRXV4, ALR, ALR1, ALR2, AR, ARI, BAR, Fibroblast growth factor regulated protein, FR-1 protein, GRE3, HAR, HRAR, isobutyraldehyde reductase, More, MVDP, NADPH-aldopentose reductase, NADPH-aldose reductase, polyol dehydrogenase (NADP2), RLAR, small intestine reductase, TPN-polyol dehydrogenase, VAS deferens androgen-dependent protein, YqhD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.21 aldehyde reductase

General Stability

General Stability on EC 1.1.1.21 - aldehyde reductase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
-55°C, unstable to prolonged storage
-
25°C, 0.3 M phosphate or Tris, pH 6.0, more than 2 months
-
alpha-crystallin stabilizes the enzyme with higher efficiency at 37°C compared to 25°C
-
dithiothreitol, 2 mM stabilizes, other sulfhydryl compounds such as beta-mercaptoethanol, glutathione and cysteine are less effective
-
DTT stabilizes the purified native enzyme from lens at 2 mM
-
enzyme is not stable unless dithiothreitol in micromolar range is present
-
extremely labile to freezing and thawing unless Carbowax 100 is included, 10 mg/ml
-
freezing and thawing at temperatures above 50°C results in loss of activity
-
freezing and thawing: more than twice, unstable
-
freezing of purified enzyme results in complete loss of activity
-
needs Tween for stability during purification
-
thiol stabilizing reagents are necessary
-