Any feedback?
Literature summary for 1.1.1.21 extracted from
- D-Xylose metabolism by Candida intermedia: isolation and characterization of two forms of aldose reductase with different coenzyme specificities (2000), J. Chromatogr., 737, 195-202.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | [Candida] intermedia |
General Stability
| General Stability | Organism |
|---|---|
| thiol stabilizing reagents are necessary | [Candida] intermedia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 56 | - |
D-xylose | isozyme ALR 2, NADPH | [Candida] intermedia |  |
| 61 | - |
D-xylose | isozyme ALR 1, NADPH | [Candida] intermedia | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
[Candida] intermedia | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
2 * 36000, SDS-PAGE | [Candida] intermedia |
| 58000 | - |
ALR1 und ALR 2, theoretical molar mass 76000 Da | [Candida] intermedia |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Candida] intermedia | - |
2 isoenzymes ALR 1 und ALR 2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ALR 1 und ALR 2 | [Candida] intermedia |
| wild type and recombinant from Escherichia coli | [Candida] intermedia |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.4 | - |
isozyme ALR 2 | [Candida] intermedia |
| 12.1 | - |
recombinant expressed in Escherichia coli | [Candida] intermedia |
| 26.8 | - |
isozyme ALR 1 | [Candida] intermedia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alditol + NAD(P)+ | - |
[Candida] intermedia | aldose + NAD(P)H | - |
? | |
| aldose + NAD(P)H | wide specificity | [Candida] intermedia | alditol + NAD(P)+ | - |
? | |
| D-xylose + NAD(P)H | - |
[Candida] intermedia | xylitol + NAD(P)+ | - |
r | |
| pentose + NADPH | - |
[Candida] intermedia | pentitol + NADP+ | - |
r | |
| xylitol + NAD(P)+ | - |
[Candida] intermedia | D-xylose + NAD(P)H + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 36000, SDS-PAGE | [Candida] intermedia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | ALR 2 | [Candida] intermedia | |
| NADPH | ALR 1 strictly specific for NADPH | [Candida] intermedia | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
