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(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + dGTP
(ribonucleotide)20 + dGMP + diphosphate
(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + GTP
(ribonucleotide)20 + GMP + diphosphate
(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + ITP
(ribonucleotide)20 + IMP + diphosphate
(ribonucleotide)20-3'-phosphate + 5'-hydroxy-(ribonucleotide)20 + GTP
(ribonucleotide)40 + GMP + diphosphate
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m
(ribonucleotide)n+m
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + ATP + H2O
(ribonucleotide)n+m + AMP + diphosphate
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + CTP + H2O
(ribonucleotide)n+m + CMP + diphosphate
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + UTP + H2O
(ribonucleotide)n+m + UMP + diphosphate
(ribonucleotide)n-2',3'-cyclophosphate + H2O
(ribonucleotide)n-3'-phosphate
(ribonucleotide)n-3'-(5'-diphosphoguanosine) + 5'-hydroxy-(ribonucleotide)m
(ribonucleotide)n+m + GMP
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 2-aminopurine-riboside-5'-triphosphate
(ribonucleotide)n+m + 2-aminopurine-riboside-5'-monophosphate + diphosphate
-
about 20% activity with 0.1 mM 2-aminopurine triphosphate compared to GTP
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 2-mercaptopurine-riboside-5'-triphosphate
(ribonucleotide)n+m + 2-mercaptopurine-riboside-5'-monophosphate + diphosphate
-
about 25% activity with 0.1 mM 2-mercaptopurine triphosphate compared to GTP
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 6-chloropurine-riboside-5'-triphosphate
(ribonucleotide)n+m + 6-chloropurine-riboside-5'-monophosphate + diphosphate
-
about 1% activity with 0.1 mM 6-chloropurine triphosphate compared to GTP
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 6-O-methylguanosine triphosphate
(ribonucleotide)n+m + 6-O-methylguanosine phosphate + diphosphate
-
about 45% activity with 0.1 mM 6-O-methyl triphosphate compared to GTP
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + ATP
(ribonucleotide)n+m + AMP + diphosphate
-
about 22% activity with 0.1 mM ATP compared to GTP
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + CTP
(ribonucleotide)n+m + CMP + diphosphate
-
about 18% activity with 0.1 mM CTP compared to GTP
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
(ribonucleotide)n+m + GMP + diphosphate
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + ITP
(ribonucleotide)n+m + IMP + diphosphate
-
about 75% activity with 0.1 mM ITP compared to GTP
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + UTP
(ribonucleotide)n+m + UMP + diphosphate
-
about 85% activity with 0.1 mM UTP compared to GTP
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-phospho-(ribonucleotide)m + GTP
(ribonucleotide)n+m + GMP + diphosphate
-
overall reaction
-
?
(ribonucleotide)n-3'-phosphate + GTP
? + GMP
(truncated 16S rRNA)-3'-phosphate + 5'-hydroxy-RNA43 + GTP
16S rRNA + GMP + diphosphate
-
endoribonuclease MazF, the toxin component of the toxin-antitoxin module mazEF, specifically cleaves the 16S rRNA of 70S ribosomes at an ACA site located at positions 15001502. Thereby, the ribosome loses a 3'-terminal 16S rRNA fragment of 43 nucleotides. RNA ligase RtcB catalyzes the religation of the truncated 16S rRNA present in specialized ribosomes. Thereby their ability to translate canonical mRNAs is fully restored
-
-
?
5'-guanosyl [RNA ligase]-NT-phosphono-L-histidine + (ribonucleotide)n-3'-phosphate
(ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine
-
RtcB transfers GMP to the RNA 3'-phosphate end
-
?
5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + (ribonucleotide)n-3'-phosphate
(ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine
GTP + [RNA ligase]-L-histidine
5'-guanosyl [RNA ligase]-NT-phosphono-L-histidine + diphosphate
-
-
-
?
GTP + [RNA ligase]-L-histidine
5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + diphosphate
additional information
?
-
(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + dGTP
(ribonucleotide)20 + dGMP + diphosphate
in the absence of Archease, RtcB-catalyzed RNA ligation proceeds efficiently with GTP and substantially less efficiently with dGTP and ITP. Inclusion of Archease in ligation reaction mixtures enables efficient utilization of GTP, dGTP, ATP or ITP
-
-
?
(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + dGTP
(ribonucleotide)20 + dGMP + diphosphate
in the absence of Archease, RtcB-catalyzed RNA ligation proceeds efficiently with GTP and substantially less efficiently with dGTP and ITP. Inclusion of Archease in ligation reaction mixtures enables efficient utilization of GTP, dGTP, ATP or ITP
-
-
?
(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + GTP
(ribonucleotide)20 + GMP + diphosphate
in the absence of Archease, RtcB-catalyzed RNA ligation proceeds efficiently with GTP and substantially less efficiently with dGTP and ITP. Inclusion of Archease in ligation reaction mixtures enables efficient utilization of GTP, dGTP, ATP or ITP
-
-
?
(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + GTP
(ribonucleotide)20 + GMP + diphosphate
in the absence of Archease, RtcB-catalyzed RNA ligation proceeds efficiently with GTP and substantially less efficiently with dGTP and ITP. Inclusion of Archease in ligation reaction mixtures enables efficient utilization of GTP, dGTP, ATP or ITP
-
-
?
(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + ITP
(ribonucleotide)20 + IMP + diphosphate
in the absence of Archease, RtcB-catalyzed RNA ligation proceeds efficiently with GTP and substantially less efficiently with dGTP and ITP. Inclusion of Archease in ligation reaction mixtures enables efficient utilization of GTP, dGTP, ATP or ITP
-
-
?
(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + ITP
(ribonucleotide)20 + IMP + diphosphate
in the absence of Archease, RtcB-catalyzed RNA ligation proceeds efficiently with GTP and substantially less efficiently with dGTP and ITP. Inclusion of Archease in ligation reaction mixtures enables efficient utilization of GTP, dGTP, ATP or ITP
-
-
?
(ribonucleotide)20-3'-phosphate + 5'-hydroxy-(ribonucleotide)20 + GTP
(ribonucleotide)40 + GMP + diphosphate
-
-
overall reaction, phosphodiester bond synthesis is rate limiting
-
?
(ribonucleotide)20-3'-phosphate + 5'-hydroxy-(ribonucleotide)20 + GTP
(ribonucleotide)40 + GMP + diphosphate
-
-
overall reaction
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m
(ribonucleotide)n+m
generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease
RtcB-catalyzed ligation incorporates the phosphate of the cyclic phosphate into the phosphodiester bond. ATP or GTP is not required
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m
(ribonucleotide)n+m
generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease
RtcB-catalyzed ligation incorporates the phosphate of the cyclic phosphate into the phosphodiester bond. ATP or GTP is not required
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m
(ribonucleotide)n+m
generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m
(ribonucleotide)n+m
generation of substrate by cleavage of the intron-containing pre-tRNA with the Methanocaldococcus jannaschii tRNA splicing endonuclease
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + ATP + H2O
(ribonucleotide)n+m + AMP + diphosphate
about 78% activity with 0.1 mM ATP compared to GTP
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + ATP + H2O
(ribonucleotide)n+m + AMP + diphosphate
about 60% activity with 0.1 mM ATP compared to GTP
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + ATP + H2O
(ribonucleotide)n+m + AMP + diphosphate
about 60% activity with 0.1 mM ATP compared to GTP
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + CTP + H2O
(ribonucleotide)n+m + CMP + diphosphate
about 70% activity with 0.1 mM CTP compared to GTP
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + CTP + H2O
(ribonucleotide)n+m + CMP + diphosphate
about 60% activity with 0.1 mM CTP compared to GTP
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + CTP + H2O
(ribonucleotide)n+m + CMP + diphosphate
about 60% activity with 0.1 mM CTP compared to GTP
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
-
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
about 80% activity with 0.01 mM GTP
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
-
-
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
-
-
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
-
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
about 75% activity with 0.01 mM GTP
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
-
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
about 75% activity with 0.01 mM GTP
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
-
-
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
substrate for ligation is a 20mer RNA strand with 5'-OH and 2',3'-cyclic phosphate ends
overall reaction
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
substrate mimicks the broken tRNAGlu(UUC) anticodon stem-loop generated by Kluyveromyces lactis gamma-toxin, leaving 2',3'-cyclic phosphate and 5'-OH ends
overall reaction
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
-
-
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
-
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
(ribonucleotide)n+m + GMP + diphosphate
-
overall reaction
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + UTP + H2O
(ribonucleotide)n+m + UMP + diphosphate
about 75% activity with 0.1 mM UTP compared to GTP
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + UTP + H2O
(ribonucleotide)n+m + UMP + diphosphate
about 60% activity with 0.1 mM UTP compared to GTP
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + UTP + H2O
(ribonucleotide)n+m + UMP + diphosphate
about 60% activity with 0.1 mM UTP compared to GTP
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + H2O
(ribonucleotide)n-3'-phosphate
-
-
-
?
(ribonucleotide)n-2',3'-cyclophosphate + H2O
(ribonucleotide)n-3'-phosphate
-
-
-
?
(ribonucleotide)n-3'-(5'-diphosphoguanosine) + 5'-hydroxy-(ribonucleotide)m
(ribonucleotide)n+m + GMP
-
-
-
?
(ribonucleotide)n-3'-(5'-diphosphoguanosine) + 5'-hydroxy-(ribonucleotide)m
(ribonucleotide)n+m + GMP
-
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
(ribonucleotide)n+m + GMP + diphosphate
-
-
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
(ribonucleotide)n+m + GMP + diphosphate
-
overall reaction, RtcB is required for ligation of endogenous intron-containing pre-tRNAs
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
(ribonucleotide)n+m + GMP + diphosphate
-
-
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
(ribonucleotide)n+m + GMP + diphosphate
-
100% activity with 0.01 mM GTP
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
(ribonucleotide)n+m + GMP + diphosphate
-
-
-
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
(ribonucleotide)n+m + GMP + diphosphate
-
overall reaction, substrate 5'-hydroxy-(ribonucleotide)m can be composed of all ribonucleotides or all deoxynucleotides
-
?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
(ribonucleotide)n+m + GMP + diphosphate
-
-
-
-
?
(ribonucleotide)n-3'-phosphate + GTP
? + GMP
substrate is a 20-mer HORNAp
RtcB1 efficiently converts the linear substrate to a circular RNA species as a consequence of intramolecular ligation plus multimers
-
?
(ribonucleotide)n-3'-phosphate + GTP
? + GMP
substrate is a 20-mer HORNAp
RtcB2 efficiently converts the linear substrate to a circular RNA species as a consequence of intramolecular ligation plus multimers. Isoform Rtcb2 is 5fold less effectively than isoform RtcB1
-
?
(ribonucleotide)n-3'-phosphate + GTP
? + GMP
substrate is a 20-mer HORNAp
RtcB1 efficiently converts the linear substrate to a circular RNA species as a consequence of intramolecular ligation plus multimers
-
?
(ribonucleotide)n-3'-phosphate + GTP
? + GMP
substrate is a 20-mer HORNAp
RtcB2 efficiently converts the linear substrate to a circular RNA species as a consequence of intramolecular ligation plus multimers. Isoform Rtcb2 is 5fold less effectively than isoform RtcB1
-
?
5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + (ribonucleotide)n-3'-phosphate
(ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine
-
-
-
?
5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + (ribonucleotide)n-3'-phosphate
(ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine
substrate for ligation is a 20-mer RNA strand with 5'-OH and 3'-monophosphate ends
-
-
?
5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + (ribonucleotide)n-3'-phosphate
(ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine
-
-
-
?
GTP + [RNA ligase]-L-histidine
5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + diphosphate
-
-
-
?
GTP + [RNA ligase]-L-histidine
5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + diphosphate
-
-
-
?
additional information
?
-
-
ATP and CTP are at least 3 orders of magnitude less effective as phosphate donors compared to GTP
-
-
?
additional information
?
-
RtcB executes a three-step ligation pathway, consisting of reaction of His337 of the enzyme with GTP to form a covalent RtcB-(histidinyl-N)-GMP intermediate, transfer of guanylate to a polynucleotide 3'-phosphate to form a polynucleotide-(3')pp(5')G intermediate, and (iii) attack of a 5'-OH on the N(3')pp(5')G end to form the splice junction. Presence of GTP is required
-
-
?
additional information
?
-
RtcB seals broken tRNA-like stem-loop structures with 2',3'-cyclic phosphate and 5'-OH ends to form a splice junction with a 2'-OH, 3',5'-phosphodiester
-
-
?
additional information
?
-
sealing of a 2',3'-cyclic phosphate end by RtcB requires GTP, is contingent on formation of the RtcBGMP adduct, and involves a kinetically valid RNA(3')pp(5')G intermediate. RtcB catalyzes the hydrolysis of a 2',3'-cyclic phosphate to a 3'-phosphate at a rate that is at least as fast as the rate of ligation. NTP requirement for ligation is satisfied by GTP and 6-O-methyl guanosine triphosphate and inosine triphosphate. 6-chloropurine ribonucleoside triphosphate, 2-aminopurine ribonucleoside triphosphate and ATP are inactive
-
-
?
additional information
?
-
-
the enzyme joins RNA 2',3'-cyclic phosphate or RNA 3'-phosphate ends to 5'-OH RNA ends in a multistep pathway whereby the enzyme hydrolyzes RNA 2',3'-cyclic phosphate to RNA 3'-phosphate, transfers GMP from GTP to RNA 3'-phosphate to form to RNAppG, and directs the attack of 5'-OH on RNAppG to form a 3'-5' phosphodiester splice junction
-
-
?
additional information
?
-
isoform Rtcb1 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand
-
-
?
additional information
?
-
isoform Rtcb1 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand
-
-
?
additional information
?
-
-
isoform Rtcb1 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand
-
-
?
additional information
?
-
isoform Rtcb2 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand
-
-
?
additional information
?
-
isoform Rtcb2 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand
-
-
?
additional information
?
-
-
isoform Rtcb2 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand
-
-
?
additional information
?
-
isoform Rtcb1 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand
-
-
?
additional information
?
-
isoform Rtcb1 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand
-
-
?
additional information
?
-
isoform Rtcb2 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand
-
-
?
additional information
?
-
isoform Rtcb2 displays DNA ligase and DNA 3'-capping activities on a 12-mer pDNAp strand
-
-
?
additional information
?
-
three-step mechanism for catalysis of the ligation of 3'-P and 5'-OH RNA termini by RtcB. In the activation step, cleavage of the phosphoanhydride bond between the alpha and beta phosphoryl groups of GTP occurs during transfer of GMP to the 3'-P terminus of an RNA strand to form a guanylylate-activated intermediate. In the cyclization step, the 2'-OH on the terminal ribose attacks the GMP-activated 3'-P to generate a 2',3'-cyclic phosphate and release GMP. In the ligation step, the 5'-OH terminus of a second RNA strand attacks the cyclic phosphate to form a 3',5'-phosphodiester bond
-
-
?
additional information
?
-
-
no cosubstrate: ATP
-
-
?
additional information
?
-
-
no cosubstrate: ATP
-
-
?
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Tanaka, N.; Shuman, S.
RtcB is the RNA ligase component of an Escherichia coli RNA repair operon
J. Biol. Chem.
286
7727-7731
2011
Escherichia coli (P46850)
brenda
Desai, K.K.; Raines, R.T.
tRNA ligase catalyzes the GTP-dependent ligation of RNA with 3-phosphate and 5-hydroxyl termini
Biochemistry
51
1333-1335
2012
Pyrococcus furiosus (Q8U0H4)
brenda
Desai, K.K.; Bingman, C.A.; Phillips, G.N.; Raines, R.T.
Structures of the noncanonical RNA ligase RtcB reveal the mechanism of histidine guanylylation
Biochemistry
52
2518-2525
2013
Pyrococcus furiosus (Q8U0H4)
brenda
Poothong, J.; Tirasophon, W.; Kaufman, R.
Functional analysis of the mammalian RNA ligase for IRE1 in the unfolded protein response
Biosci. Rep.
37
BSR20160574
2017
Escherichia coli, Homo sapiens (Q9Y3I0)
brenda
Kosmaczewski, S.G.; Edwards, T.J.; Han, S.M.; Eckwahl, M.J.; Meyer, B.I.; Peach, S.; Hesselberth, J.R.; Wolin, S.L.; Hammarlund, M.
The RtcB RNA ligase is an essential component of the metazoan unfolded protein response
EMBO Rep.
15
1278-1285
2014
Caenorhabditis elegans (P90838), Caenorhabditis elegans
brenda
Maughan, W.P.; Shuman, S.
Characterization of 3-phosphate RNA ligase paralogs RtcB1, RtcB2, and RtcB3 from Myxococcus xanthus highlights DNA and RNA 5'-phosphate capping activity of RtcB3
J. Bacteriol.
197
3616-3624
2015
Myxococcus xanthus (Q1D2I5), Myxococcus xanthus (Q1DFL2), Myxococcus xanthus, Myxococcus xanthus DK 1622 (Q1D2I5), Myxococcus xanthus DK 1622 (Q1DFL2)
brenda
Tanaka, N.; Meineke, B.; Shuman, S.
RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo
J. Biol. Chem.
286
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Escherichia coli (P46850)
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Tanaka, N.; Chakravarty, A.K.; Maughan, B.; Shuman, S.
Novel mechanism of RNA repair by RtcB via sequential 2',3'-cyclic phosphodiesterase and 3'-phosphate/5'-hydroxyl ligation reactions
J. Biol. Chem.
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Escherichia coli (P46850)
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Lu, Y.; Liang, F.X.; Wang, X.
A synthetic biology approach identifies the mammalian UPR RNA ligase RtcB
Mol. Cell
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758-770
2014
Mus musculus (Q99LF4)
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Chakravarty, A.K.; Shuman, S.
The sequential 2',3'-cyclic phosphodiesterase and 3'-phosphate/5'-OH ligation steps of the RtcB RNA splicing pathway are GTP-dependent
Nucleic Acids Res.
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2012
Escherichia coli (P46850)
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Desai, K.K.; Cheng, C.L.; Bingman, C.A.; Phillips, G.N.; Raines, R.T.
A tRNA splicing operon: Archease endows RtcB with dual GTP/ATP cofactor specificity and accelerates RNA ligation
Nucleic Acids Res.
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Pyrococcus horikoshii (O59245), Pyrococcus horikoshii DSM 12428 (O59245)
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Temmel, H.; Mueller, C.; Sauert, M.; Vesper, O.; Reiss, A.; Popow, J.; Martinez, J.; Moll, I.
The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli
Nucleic Acids Res.
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2017
Escherichia coli
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Englert, M.; Sheppard, K.; Aslanian, A.; Yates, J.R.; Soell, D.
Archaeal 3-phosphate RNA splicing ligase characterization identifies the missing component in tRNA maturation
Proc. Natl. Acad. Sci. USA
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2011
Pyrococcus horikoshii (O59245), Methanopyrus kandleri (Q8TUS2), Pyrobaculum aerophilum (Q8ZY09), Pyrobaculum aerophilum DSM 7523 (Q8ZY09), Pyrococcus horikoshii DSM 12428 (O59245), Methanopyrus kandleri DSM 6324 (Q8TUS2)
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Englert, M.; Xia, S.; Okada, C.; Nakamura, A.; Tanavde, V.; Yao, M.; Eom, S.H.; Konigsberg, W.H.; Soell, D.; Wang, J.
Structural and mechanistic insights into guanylylation of RNA-splicing ligase RtcB joining RNA between 3-terminal phosphate and 5-OH
Proc. Natl. Acad. Sci. USA
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15235-15240
2012
Pyrococcus horikoshii (O59245), Pyrococcus horikoshii, Pyrococcus horikoshii DSM 12428 (O59245)
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Chakravarty, A.K.; Subbotin, R.; Chait, B.T.; Shuman, S.
RNA ligase RtcB splices 3-phosphate and 5-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3)pp(5)G intermediates
Proc. Natl. Acad. Sci. USA
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6072-6077
2012
Escherichia coli (P46850)
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Desai, K.K.; Beltrame, A.L.; Raines, R.T.
Coevolution of RtcB and Archease created a multiple-turnover RNA ligase
RNA
21
1866-1872
2015
Thermus thermophilus, Thermobifida fusca
brenda
Maughan, W.; Shuman, S.
Distinct contributions of enzymic functional groups to the 2',3'-cyclic phosphodiesterase, 3'-phosphate guanylylation, and 3'-ppG/5'-OH ligation steps of the Escherichia coli RtcB nucleic acid splicing pathway
J. Bacteriol.
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Escherichia coli
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Ray, A.; Zhang, S.; Rentas, C.; Caldwell, K.A.; Caldwell, G.A.
RTCB-1 mediates neuroprotection via XBP-1 mRNA splicing in the unfolded protein response pathway
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Caenorhabditis elegans
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Popow, J.; Jurkin, J.; Schleiffer, A.; Martinez, J.
Analysis of orthologous groups reveals archease and DDX1 as tRNA splicing factors
Nature
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2014
Homo sapiens
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Peach, S.E.; York, K.; Hesselberth, J.R.
Global analysis of RNA cleavage by 5-hydroxyl RNA sequencing
Nucleic Acids Res.
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e108
2015
Escherichia coli
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Remus, B.S.; Goldgur, Y.; Shuman, S.
Structural basis for the GTP specificity of the RNA kinase domain of fungal tRNA ligase
Nucleic Acids Res.
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12945-12953
2017
Candida albicans
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Kosmaczewski, S.; Han, S.; Han, B.; Meyer, B.; Baig, H.; Athar, W.; Lin-Moore, A.; Koelle, M.; Hammarlund, M.
RNA ligation in neurons by RtcB inhibits axon regeneration
Proc. Natl. Acad. Sci. USA
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8451-8456
2015
Caenorhabditis elegans
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Nandy, A.; Saenz-Mendez, P.; Gorman, A.M.; Samali, A.; Eriksson, L.A.
Homology model of the human tRNA splicing ligase RtcB
Proteins
85
1983-1993
2017
Homo sapiens (Q9Y3I0)
brenda
Remus, B.S.; Schwer, B.; Shuman, S.
Characterization of the tRNA ligases of pathogenic fungi Aspergillus fumigatus and Coccidioides immitis
RNA
22
1500-1509
2016
Coccidioides immitis (J3K151), Aspergillus fumigatus (Q4WTD4), Coccidioides immitis RS (J3K151)
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