Protein Variants | Comment | Organism |
---|---|---|
C78A | completely inactive | Escherichia coli |
D75A | the mutation allows cyclic phosphodiester hydrolysis but cripples 3'-phosphate guanylylation | Escherichia coli |
H168A | the mutant shows about 48% of 5'-OH-RNAp ligation activity compared to the wild type enzyme | Escherichia coli |
H185A | the mutant shows 5'-OH-RNAp ligation activity similar to the wild type enzyme | Escherichia coli |
H281A | the mutant is impaired in overall 5'-OH-RNAp and 5'-OH-RNA-2',3'-cyclic phosphate ligation but is able to seal a preguanylylated substrate | Escherichia coli |
H337A | the mutant shows about 1% of 5'-OH-RNAp ligation activity compared to the wild type enzyme | Escherichia coli |
K299A | the mutant shows about 50% of 5'-OH-RNAp ligation activity compared to the wild type enzyme | Escherichia coli |
N167A | the mutant shows about 65% of 5'-OH-RNAp ligation activity compared to the wild type enzyme | Escherichia coli |
R189A | the mutation slows the step of RNAppG/5'-OH RNA sealing by a factor of 200 compared to that with wild type enzyme while decreasing the rate of RNAppG formation by 3fold | Escherichia coli |
R341A | the mutant shows about 10% of 5'-OH-RNAp ligation activity compared to the wild type enzyme | Escherichia coli |
R345A | the mutant shows 5'-OH-RNAp ligation activity similar to the wild type enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O | Escherichia coli | - |
(ribonucleotide)n+m + GMP + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP | Escherichia coli | - |
(ribonucleotide)n+m + GMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-agarose column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O | - |
Escherichia coli | (ribonucleotide)n+m + GMP + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP | - |
Escherichia coli | (ribonucleotide)n+m + GMP + diphosphate | - |
? | |
additional information | the enzyme joins RNA 2',3'-cyclic phosphate or RNA 3'-phosphate ends to 5'-OH RNA ends in a multistep pathway whereby the enzyme hydrolyzes RNA 2',3'-cyclic phosphate to RNA 3'-phosphate, transfers GMP from GTP to RNA 3'-phosphate to form to RNAppG, and directs the attack of 5'-OH on RNAppG to form a 3'-5' phosphodiester splice junction | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
rtcB | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
GTP | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme operates during the unfolded protein response | Escherichia coli |