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Literature summary for 6.5.1.8 extracted from

  • Remus, B.S.; Schwer, B.; Shuman, S.
    Characterization of the tRNA ligases of pathogenic fungi Aspergillus fumigatus and Coccidioides immitis (2016), RNA, 22, 1500-1509 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in a Saccharomyces cerevisiae trl1DELTA mutant Coccidioides immitis
expressed in a Saccharomyces cerevisiae trl1DELTA mutant Aspergillus fumigatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Coccidioides immitis
Mg2+ required Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O Coccidioides immitis
-
 (ribonucleotide)n+m + GMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O Aspergillus fumigatus
-
 (ribonucleotide)n+m + GMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O Coccidioides immitis RS
-
 (ribonucleotide)n+m + GMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q4WTD4
-
-
Coccidioides immitis J3K151
-
-
Coccidioides immitis RS J3K151
-
-

Purification (Commentary)

Purification (Comment) Organism
His60 Ni Superflow resin column chromatography and Superose-200 gel filtration Coccidioides immitis
His60 Ni Superflow resin column chromatography and Superose-200 gel filtration Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + ATP + H2O about 60% activity with 0.1 mM ATP compared to GTP Coccidioides immitis (ribonucleotide)n+m + AMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + ATP + H2O about 78% activity with 0.1 mM ATP compared to GTP Aspergillus fumigatus (ribonucleotide)n+m + AMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + ATP + H2O about 60% activity with 0.1 mM ATP compared to GTP Coccidioides immitis RS (ribonucleotide)n+m + AMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + CTP + H2O about 60% activity with 0.1 mM CTP compared to GTP Coccidioides immitis (ribonucleotide)n+m + CMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + CTP + H2O about 70% activity with 0.1 mM CTP compared to GTP Aspergillus fumigatus (ribonucleotide)n+m + CMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + CTP + H2O about 60% activity with 0.1 mM CTP compared to GTP Coccidioides immitis RS (ribonucleotide)n+m + CMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
-
 Coccidioides immitis (ribonucleotide)n+m + GMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
-
 Aspergillus fumigatus (ribonucleotide)n+m + GMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O about 75% activity with 0.01 mM GTP Coccidioides immitis (ribonucleotide)n+m + GMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O about 80% activity with 0.01 mM GTP Aspergillus fumigatus (ribonucleotide)n+m + GMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
-
 Coccidioides immitis RS (ribonucleotide)n+m + GMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O about 75% activity with 0.01 mM GTP Coccidioides immitis RS (ribonucleotide)n+m + GMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + UTP + H2O about 60% activity with 0.1 mM UTP compared to GTP Coccidioides immitis (ribonucleotide)n+m + UMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + UTP + H2O about 75% activity with 0.1 mM UTP compared to GTP Aspergillus fumigatus (ribonucleotide)n+m + UMP + diphosphate
-
 ?
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + UTP + H2O about 60% activity with 0.1 mM UTP compared to GTP Coccidioides immitis RS (ribonucleotide)n+m + UMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 94200, calculated from amino acid sequence Aspergillus fumigatus
monomer 1 * 942400, calculated from amino acid sequence Coccidioides immitis

Synonyms

Synonyms Comment Organism
Trl1
-
 Coccidioides immitis
Trl1
-
 Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
GTP preferred cofactor Coccidioides immitis
GTP preferred cofactor Aspergillus fumigatus

General Information

General Information Comment Organism
physiological function the enzyme operates during the unfolded protein response Coccidioides immitis
physiological function the enzyme operates during the unfolded protein response Aspergillus fumigatus