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Literature summary for 6.5.1.8 extracted from

  • Desai, K.K.; Raines, R.T.
    tRNA ligase catalyzes the GTP-dependent ligation of RNA with 3-phosphate and 5-hydroxyl termini (2012), Biochemistry, 51, 1333-1335.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
C98A mutation in predicted metal-binding site, loss of activity Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ absolutely required Pyrococcus furiosus
additional information Zn2+ cannot replace Mn2+ Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U0H4
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
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 Pyrococcus furiosus (ribonucleotide)n+m + GMP + diphosphate overall reaction ?
(ribonucleotide)n-2',3'-cyclophosphate + H2O
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 Pyrococcus furiosus (ribonucleotide)n-3'-phosphate
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 ?
(ribonucleotide)n-3'-(5'-diphosphoguanosine) + 5'-hydroxy-(ribonucleotide)m
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 Pyrococcus furiosus (ribonucleotide)n+m + GMP
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 ?
5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + (ribonucleotide)n-3'-phosphate
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 Pyrococcus furiosus (ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine
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 ?
GTP + [RNA ligase]-L-histidine
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 Pyrococcus furiosus 5'-guanosyl [RNA ligase]-Ntau-phosphono-L-histidine + diphosphate
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 ?
additional information three-step mechanism for catalysis of the ligation of 3'-P and 5'-OH RNA termini by RtcB. In the activation step, cleavage of the phosphoanhydride bond between the alpha and beta phosphoryl groups of GTP occurs during transfer of GMP to the 3'-P terminus of an RNA strand to form a guanylylate-activated intermediate. In the cyclization step, the 2'-OH on the terminal ribose attacks the GMP-activated 3'-P to generate a 2',3'-cyclic phosphate and release GMP. In the ligation step, the 5'-OH terminus of a second RNA strand attacks the cyclic phosphate to form a 3',5'-phosphodiester bond Pyrococcus furiosus ?
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 ?

Synonyms

Synonyms Comment Organism
rtcB
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 Pyrococcus furiosus
tRNA-splicing ligase
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 Pyrococcus furiosus

General Information

General Information Comment Organism
physiological function enzyme catalyzes the GTP-dependent ligation of RNA with 3'-phosphate and 5'-hydroxyl termini. RtcB can ligate RNA cleaved by RNA endonucleases, which generate 2',3'-cyclic phosphate and then 3'-phosphate termini on one strand, and a 5'-hydroxyl terminus on another strand Pyrococcus furiosus