Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O | Escherichia coli | - |
(ribonucleotide)n+m + GMP + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP | Escherichia coli | - |
(ribonucleotide)n+m + GMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and HiTrap heparin column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O | - |
Escherichia coli | (ribonucleotide)n+m + GMP + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP | - |
Escherichia coli | (ribonucleotide)n+m + GMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
RtcB RNA ligase | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme operates during the unfolded protein response | Escherichia coli |