Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermobifida fusca |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | assay in presence of Mn2+ | Thermus thermophilus | |
Mn2+ | assay in presence of Mn2+ | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ribonucleotide)20-3'-phosphate + 5'-hydroxy-(ribonucleotide)20 + GTP | - |
Thermus thermophilus | (ribonucleotide)40 + GMP + diphosphate | overall reaction | ? | |
(ribonucleotide)20-3'-phosphate + 5'-hydroxy-(ribonucleotide)20 + GTP | - |
Thermobifida fusca | (ribonucleotide)40 + GMP + diphosphate | overall reaction, phosphodiester bond synthesis is rate limiting | ? | |
additional information | no cosubstrate: ATP | Thermus thermophilus | ? | - |
? | |
additional information | no cosubstrate: ATP | Thermobifida fusca | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
(ribonucleotide)20-3'-phosphate | pH 7.4, 45°C | Thermobifida fusca | |
0.0043 | - |
(ribonucleotide)20-3'-phosphate | pH 7.4, 70°C | Thermus thermophilus | |
0.0122 | - |
(ribonucleotide)20-3'-phosphate | pH 7.4, 70°C, presence of archease | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
physiological function | RtcB from Thermobifida fusca is a single-turnover enzyme that is unable to be converted into a multiple-turnover ligase by archease from other organisms | Thermobifida fusca |
physiological function | RtcB from Thermus thermophilus displays a strong dependency on Archease for maximal activity and can catalyze multiple turnovers only in the presence of archease. Archease from P. horikoshii can activate Thermus thermophilus RtcB | Thermus thermophilus |