Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.5.1.8 extracted from

  • Desai, K.K.; Beltrame, A.L.; Raines, R.T.
    Coevolution of RtcB and Archease created a multiple-turnover RNA ligase (2015), RNA, 21, 1866-1872.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermobifida fusca

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ assay in presence of Mn2+ Thermus thermophilus
Mn2+ assay in presence of Mn2+ Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ribonucleotide)20-3'-phosphate + 5'-hydroxy-(ribonucleotide)20 + GTP
-
Thermus thermophilus (ribonucleotide)40 + GMP + diphosphate overall reaction ?
(ribonucleotide)20-3'-phosphate + 5'-hydroxy-(ribonucleotide)20 + GTP
-
Thermobifida fusca (ribonucleotide)40 + GMP + diphosphate overall reaction, phosphodiester bond synthesis is rate limiting ?
additional information no cosubstrate: ATP Thermus thermophilus ?
-
?
additional information no cosubstrate: ATP Thermobifida fusca ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0014
-
(ribonucleotide)20-3'-phosphate pH 7.4, 45°C Thermobifida fusca
0.0043
-
(ribonucleotide)20-3'-phosphate pH 7.4, 70°C Thermus thermophilus
0.0122
-
(ribonucleotide)20-3'-phosphate pH 7.4, 70°C, presence of archease Thermus thermophilus

General Information

General Information Comment Organism
physiological function RtcB from Thermobifida fusca is a single-turnover enzyme that is unable to be converted into a multiple-turnover ligase by archease from other organisms Thermobifida fusca
physiological function RtcB from Thermus thermophilus displays a strong dependency on Archease for maximal activity and can catalyze multiple turnovers only in the presence of archease. Archease from P. horikoshii can activate Thermus thermophilus RtcB Thermus thermophilus