Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus(DE3) cells | Candida albicans |
Crystallization (Comment) | Organism |
---|---|
in complex with GDP and Mg2+, sitting drop vapor diffusion method, using 0.2 M sodium dihydrogen phosphate, 20% (w/v) PEG3350 | Candida albicans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O | Candida albicans | - |
(ribonucleotide)n+m + GMP + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP | Candida albicans | - |
(ribonucleotide)n+m + GMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography and gel filtration | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O | - |
Candida albicans | (ribonucleotide)n+m + GMP + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 2-aminopurine-riboside-5'-triphosphate | about 20% activity with 0.1 mM 2-aminopurine triphosphate compared to GTP | Candida albicans | (ribonucleotide)n+m + 2-aminopurine-riboside-5'-monophosphate + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 2-mercaptopurine-riboside-5'-triphosphate | about 25% activity with 0.1 mM 2-mercaptopurine triphosphate compared to GTP | Candida albicans | (ribonucleotide)n+m + 2-mercaptopurine-riboside-5'-monophosphate + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 6-chloropurine-riboside-5'-triphosphate | about 1% activity with 0.1 mM 6-chloropurine triphosphate compared to GTP | Candida albicans | (ribonucleotide)n+m + 6-chloropurine-riboside-5'-monophosphate + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 6-O-methylguanosine triphosphate | about 45% activity with 0.1 mM 6-O-methyl triphosphate compared to GTP | Candida albicans | (ribonucleotide)n+m + 6-O-methylguanosine phosphate + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + ATP | about 22% activity with 0.1 mM ATP compared to GTP | Candida albicans | (ribonucleotide)n+m + AMP + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + CTP | about 18% activity with 0.1 mM CTP compared to GTP | Candida albicans | (ribonucleotide)n+m + CMP + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP | - |
Candida albicans | (ribonucleotide)n+m + GMP + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP | 100% activity with 0.01 mM GTP | Candida albicans | (ribonucleotide)n+m + GMP + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + ITP | about 75% activity with 0.1 mM ITP compared to GTP | Candida albicans | (ribonucleotide)n+m + IMP + diphosphate | - |
? | |
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + UTP | about 85% activity with 0.1 mM UTP compared to GTP | Candida albicans | (ribonucleotide)n+m + UMP + diphosphate | - |
? | |
additional information | ATP and CTP are at least 3 orders of magnitude less effective as phosphate donors compared to GTP | Candida albicans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Trl1 | - |
Candida albicans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
GTP | - |
Candida albicans |
General Information | Comment | Organism |
---|---|---|
physiological function | fungal tRNA ligase Trl1 is an essential enzyme that repairs RNA breaks with 2',3'-cyclic-PO4 and 5'-OH ends inflicted during tRNA splicing and noncanonical mRNA splicing in the fungal unfolded protein response | Candida albicans |