Information on EC 2.8.2.15 - steroid sulfotransferase

for references in articles please use BRENDA:EC2.8.2.15
Word Map on EC 2.8.2.15
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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.8.2.15
-
RECOMMENDED NAME
GeneOntology No.
steroid sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + a phenolic steroid = adenosine 3',5'-bisphosphate + steroid O-sulfate
show the reaction diagram
broad specificity; broad specificity resembling EC 2.8.2.2 alcohol sulfotransferase, but also acts on estrone
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Steroid hormone biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:phenolic-steroid sulfotransferase
Broad specificity resembling EC 2.8.2.2 alcohol sulfotransferase, but also acts on estrone.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-76-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bovine
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
knockdown of SULT2B1 in Hepa1-6 cells induces cell-cycle arrest and apoptosis and suppresses tumorigenesis in vivo
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + 3alpha-hydroxytibolone
adenosine 3',5'-bisphosphate + 3alpha-hydroxytibolone 3-O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + 3beta-hydroxytibolone
adenosine 3',5'-bisphosphate + 3beta-hydroxytibolone 3-O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + 4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl
adenosine 3',5'-bisphosphate + 4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl 4'-O-sulfate
show the reaction diagram
-
i.e. 4'-OH PCB 68, substrate of SULT1A2
-
-
?
3'-phosphoadenylyl sulfate + 4-hydroxy-2',3,5-trichlorobiphenyl
adenosine 3',5'-bisphosphate + 2',3,5-trichlorobiphenyl-4-yl hydrogen sulfate
show the reaction diagram
-
i.e. 4-OH PCB 34, substrate of SULT1A2
-
-
?
3'-phosphoadenylyl sulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + 5alpha,6alpha-epoxycholesterol
adenosine 3',5'-bisphosphate + 5alpha,6alpha-epoxycholesteryl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 5beta,6beta-epoxycholesterol
adenosine 3',5'-bisphosphate + 5beta,6beta-epoxycholesteryl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 7-oxo-cholesterol
adenosine 3',5'-bisphosphate + 7-oxo-cholesteryl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 7alpha-hydroxycholesterol
adenosine 3',5'-bisphosphate + 7alpha-hydroxycholesteryl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 7beta-hydroxycholesterol
adenosine 3',5'-bisphosphate + 7beta-hydroxycholesteryl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + desipramine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + L-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + oxysterol
adenosine 3',5'-bisphosphate + oxysterol sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + taurolithocholate
adenosine 3',5'-bisphosphate + taurolithocholate 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + xanthurenic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + (22S,23S)-28-homobrassinolide
adenosine 3',5'-bisphosphate + (22S,23S)-28-homobrassinolide sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + (22S,23S)-28-homocastasterone
adenosine 3',5'-bisphosphate + (22S,23S)-28-homocastasterone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 11-deoxycorticosterone
adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
show the reaction diagram
-
21-hydroxypregn-4-en-3,20-dione
pregn-4-en-3-one 21-sulfate
r
3'-phosphoadenylylsulfate + 16-hydroxy-estradiol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 17alpha-estradiol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + 17alpha-ethynylestradiol
adenosine 3',5'-bisphosphate + 17alpha-ethynylestradiol 3-sulfate
show the reaction diagram
-
celecoxib switches sulfation from the 3-O-position to the 17beta-O-position
-
-
?
3'-phosphoadenylylsulfate + 17beta-estradiol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + 17beta-estradiol 3-methyl ether
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 17beta-estradiol adenosine
adenosine 3',5'-bisphosphate + 17beta-estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 2-hydroxy-estradiol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-naphthol
adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 24-epibrassinolide
adenosine 3',5'-bisphosphate + 24-epibrassinolide sulfate
show the reaction diagram
-
sulfonation leads to the loss of biological activity
-
-
r
3'-phosphoadenylylsulfate + 24-epicastasterone
adenosine 3',5'-bisphosphate + 24-epicastasterone 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 24-epicastasterone
adenosine 3',5'-bisphosphate + 24-epicastasterone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 24-epiteasterone
adenosine 3',5'-bisphosphate + 24-epiteasterone 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 24-epiteasterone
adenosine 3',5'-bisphosphate + 24-epiteasterone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3,3',5-triidothyronine
adenosine 3',5'-bisphosphate + 3,3',5-triiodothyronine 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 3alpha-hydroxy-5alpha-androstan-17-one
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 3beta-hydroxy-5alpha-androstan-17-one
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 4'-hydroxy-2',3,4-trichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4'-hydroxy-2,3',4-trichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-hydroxy-2',3,5-trichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-hydroxy-2,4'-dichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-hydroxy-3,3',5-trichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-hydroxy-3,3'-dichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-hydroxy-3,5-dichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-hydroxy-estradiol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 5alpha-androst-16-en-3beta-ol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 6-deoxo-24-epicastasterone
adenosine 3',5'-bisphosphate + 6-deoxo-24-epicastasterone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + allopregnenolone
adenosine 3',5'-bisphosphate + allopregnenolone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + androst-5-ene-3beta,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androst-5-ene-3beta17alpha-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androstenediol
adenosine 3',5'-bisphosphate + 17beta-hydroxy-androst-5-en-3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + androstenediol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
SULT2B1 isoform
-
-
r
3'-phosphoadenylylsulfate + androsterone
adenosine 3',5'-bisphosphate + 5alpha-androstane-17-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + beta-naphthol
adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + butylated hydroxy anisole
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + caffeic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + catechin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chlorogenic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol sulfate
show the reaction diagram
-
sulfurylated at low rate
-
-
r
3'-phosphoadenylylsulfate + cortisol
adenosine 3',5'-bisphosphate + cortisol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + daidzein
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + diethylstilbestrol
adenosine 3',5'-bisphosphate + diethylstilbestrol sulfate
show the reaction diagram
-
synthetic estrogen
-
r
3'-phosphoadenylylsulfate + epigallocatechin gallate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + epitestosterone
adenosine 3',5'-bisphosphate + epitestosterone sulfate
show the reaction diagram
-
17alpha-hydroxyandrost-4-en-3-one
androst-4-en-3-one 17-sulfate
r
3'-phosphoadenylylsulfate + estradiol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + estriol
adenosine 3',5'-bisphosphate + estriol sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + estrone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + estrone
adenosine 3',5'-bisphosphate + estrone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + etiocholanone
adenosine 3',5'-bisphosphate + etiocholanone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + genistein
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + glycolithocholate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + kaempferol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + myricetin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + n-propyl gallate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregn-5-en-20-one 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + quercetin
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + testosterone
adenosine 3',5'-bisphosphate + testosterone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + thyroxine
adenosyl 3',5'-bisphosphate + O-[3,5-diiodo-4-(sulfooxy)phenyl]-3,5-diiodotyrosine
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + 3,4-dihydroxyphenylacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + 3alpha-hydroxytibolone
adenosine 3',5'-bisphosphate + 3alpha-hydroxytibolone 3-O-sulfate
show the reaction diagram
-
sulfation inactivates the hydroxylated metabolite of tibolone, a synthetic steroid used for the treatment for climacteric symptoms and postmenopausal osteoporosis
-
-
?
3'-phosphoadenylyl sulfate + 3beta-hydroxytibolone
adenosine 3',5'-bisphosphate + 3beta-hydroxytibolone 3-O-sulfate
show the reaction diagram
-
sulfation inactivates the hydroxylated metabolite of tibolone, a synthetic steroid used for the treatment for climacteric symptoms and postmenopausal osteoporosis
-
-
?
3'-phosphoadenylyl sulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-O-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone O-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + desipramine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + L-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone sulfate
show the reaction diagram
Q06520
-
-
-
?
3'-phosphoadenylyl sulfate + xanthurenic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + 4'-hydroxy-2',3,4-trichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4'-hydroxy-2,3',4-trichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-hydroxy-2',3,5-trichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-hydroxy-2,4'-dichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-hydroxy-3,3',5-trichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-hydroxy-3,3'-dichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-hydroxy-3,5-dichlorobiphenyl
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + androsterone
adenosine 3',5'-bisphosphate + 5alpha-androstane-17-one 3-sulfate
show the reaction diagram
-
enzyme is an androsterone sulfotransferase as well as a dehydroepiandrosterone sulfotransferase, implying an important role in steroid homeostasis for the adrenals and liver
-
-
?
3'-phosphoadenylylsulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
show the reaction diagram
-
enzyme is an androsterone sulfotransferase as well as a dehydroepiandrosterone sulfotransferase, implying an important role in steroid homeostasis for the adrenals and liver
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
markedly activated by 5 mM divalent cations
Ca2+
-
markedly activated by 5 mM divalent cations
Co2+
-
markedly activated by 5 mM divalent cations
Cr2+
-
markedly activated by 5 mM divalent cations
Mn2+
-
most effective, markedly activated by 5 mM divalent cations
NaCl
-
activates
Ni2+
-
markedly activated by 5 mM divalent cations
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3-dichlorophenol
-
IC50: 0.0007 mM, vs. 10 nM estradiol
2,4-Dichlorophenol
-
IC50: 0.0007 mM, vs. 10 nM estradiol
2,5-dichlorophenol
-
IC50: 0.0022 mM, vs. 10 nM estradiol
2,6-Dichloro-4-nitrophenol
-
non-specific SULT inhibitor
2,6-dichlorophenol
-
IC50: 0.0016 mM, vs. 10 nM estradiol
2-chlorophenol
-
IC50: 0.02 mM, vs. 10 nM estradiol
2-phenylphenol
-
IC50: 0.0052 mM, vs. 10 nM estradiol
24-epicathasterone
-
substrate inhibition above 0.005 mM
-
24-epiteasterone
-
substrate inhibition above 0.005 mM
3',4'-dihydroxy-2,3-dichlorobiphenyl
-
-
3',4'-dihydroxy-4-monochlorobiphenyl
-
-
3,4-dichlorophenol
-
IC50: 0.013 mM, vs. 10 nM estradiol
3,5-dichlorophenol
-
IC50: 0.017 mM, vs. 10 nM estradiol
3-chlorophenol
-
IC50: 0.13 mM, vs. 10 nM estradiol
4'-hydroxy-2',3,4-trichlorobiphenyl
-
-
4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl
-
-
4'-hydroxy-2,3',4-trichlorobiphenyl
-
-
4'-hydroxy-2,3'-dichlorobiphenyl
-
-
4'-hydroxy-2,5-dichlorobiphenyl
4'-hydroxy-3,4-dichlorobiphenyl
-
-
4'-hydroxy-4-monochlorobiphenyl
-
-
4-chloro-3-methylphenol
-
IC50: 0.024 mM, vs. 10 nM estradiol
4-Chlorophenol
-
IC50: 0.11 mM, vs. 10 nM estradiol
4-hydroxy-2',3,5-trichlorobiphenyl
4-hydroxy-2,4'-dichlorobiphenyl
-
-
4-hydroxy-3,3',5-trichlorobiphenyl
-
-
4-hydroxy-3,3'-dichlorobiphenyl
-
-
4-hydroxy-3,5-dichlorobiphenyl
-
-
4-hydroxytamoxifen
-
noncompetitive inhibition
5,7-dichlorokynurenic acid
6'-hydroxy-3,3'-4-trichlorobiphenyl
-
-
adenosine 3',5'-bisphosphate
-
-
androsterone
-
stronger substrate inhibition than dehydroepiandrosterone adenosine
Cd2+
-
strongly inhibited by 5 mM
celecoxib
-
heterotrophic modulator, 3-O-sulfation is inhibited by celecoxib to the same extent, independent of the concentration of 17alpha-ethynylestradiol, switches sulfation from the 3-O-position to the 17beta-O-position
corticosterone
-
competitive inhibitor, inhibits cortisol sulfotransferase activity
cortisol
-
-
cortisone
-
-
daidzein
IC50: 0.008 mM, competitive
DCNP
-
-
dehydroepiandrosterone
deoxycorticosterone
-
competitive inhibitor, inhibits cortisol sulfotransferase activity
endoxifen
-
i.e. 4-hydroxy-N-desmethyltamoxifen, potent noncompetitive inhibition
estradiol
-
substrate inhibition above 0.002 mM
estradiol-17beta
-
competitive inhibitor, inhibits cortisol sulfotransferase activity
gavestinel
genistein
IC50: 0.0025 mM, competitive
Kynurenic acid
L689,560
L701,324
Mefenamic acid
N-demethyltamoxifen
-
competitive inhibition
p-hydroxymercuribenzoate
-
-
pregnenolone
-
substrate inhibition
progesterone
-
competitive inhibitor, inhibits cortisol sulfotransferase activity
quercetin
IC50: 0.0007 mM, competitive
tamoxifen N-oxide
-
noncompetitive inhibition
testosterone
-
competitive inhibitor, inhibits cortisol sulfotransferase activity
Zn2+
-
strongly inhibited by 5 mM
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
celecoxib
-
heterotrophic modulator, switches sulfation from the 3-O-position to the 17beta-O-position and activates such that the total sulfated product exceed product formation by the native enzyme, 3fold to 4fold at 0.25 mM celecoxib
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0012
1-naphthol
-
isoform SULT1B1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37C
0.191
17alpha-ethynylestradiol
-
-
0.00001 - 0.013
17beta-estradiol
0.0014 - 0.0049
24-epicathasterone
-
0.003 - 0.0042
24-epiteasterone
0.0003 - 0.0096
3'-phosphoadenylylsulfate
0.0036
3,3',5-triiodothyronine
-
0.0209
3,4-dihydroxyphenylacetic acid
-
isoform Sult1d1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37C
0.238
3-(10,11-dihydro-5H-dibenzo[b,f]azepin-5-yl)-N-methylpropan-1-amine
-
isoform Sult2a1, in 50 mM Tris/HCl buffer, pH 10.0, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37C
0.00079
3alpha-tibolone
-
pH 6.0, 37C, recombinant SULT1A1
0.0016 - 0.0185
3beta-hydroxyandrost-5-ene-17-one
0.00056
3beta-tibolone
-
pH 7.0, 37C, recombinant SULT1A1
0.0262
4'-hydroxy-2',3,4-trichlorobiphenyl
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.0086
4'-hydroxy-2,3',4-trichlorobiphenyl
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.0081 - 0.046
4-hydroxy-2',3,5-trichlorobiphenyl
0.0568
4-hydroxy-2,4'-dichlorobiphenyl
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.0136
4-hydroxy-3,3',5-trichlorobiphenyl
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.0366
4-hydroxy-3,3'-dichlorobiphenyl
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.0085
4-hydroxy-3,5-dichlorobiphenyl
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.0399
4-nitrophenol
-
isoform SULT1B1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37C
0.00019
allopregnanolone
-
pH 7.7, 37C, 3-alphaHST isoform
0.012
androstenediol
pH 7.5, 25C, BNST4
0.00026 - 0.0021
androsterone
0.00682
cortisol
-
pH 6.8, 37.5C
0.0006 - 0.025
dehydroepiandrosterone
0.00046
diethylstilbestrol
-
pH 7.9, 37C
0.0013
estradiol
-
pH 7.6, 37C
0.0000105 - 0.0235
estrone
0.145
L-triiodothyronine
-
isoform SULT1B1, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37C
0.0283
n-propyl gallate
-
0.00025 - 0.014
pregnenolone
0.0002
testosterone
-
pH 7.7, 37C, 3-alphaHST isoform
0.0046
thyroxine
-
0.0063 - 0.206
xanthurenic acid
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13
androsterone
-
pH 7.5, 37C
0.1
dehydroepiandrosterone
-
pH 7.5, 37C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.253
4-hydroxy-2',3,5-trichlorobiphenyl
-
pH 7.0, 37C, recombinant SULT1A2
0.0127 - 0.0194
4-hydroxytamoxifen
0.0016
adenosine 3',5'-bisphosphate
-
pH 7.5
0.0038
androsterone
-
pH 7.5, 37C
0.008 - 0.112
dehydroepiandrosterone
0.0028 - 0.0035
endoxifen
0.0098 - 0.0172
N-demethyltamoxifen
0.0096 - 0.0169
tamoxifen N-oxide
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0007
2,3-dichlorophenol
Homo sapiens
-
IC50: 0.0007 mM, vs. 10 nM estradiol
0.0007
2,4-Dichlorophenol
Homo sapiens
-
IC50: 0.0007 mM, vs. 10 nM estradiol
0.0022
2,5-dichlorophenol
Homo sapiens
-
IC50: 0.0022 mM, vs. 10 nM estradiol
0.0342 - 24
2,6-Dichloro-4-nitrophenol
0.0016
2,6-dichlorophenol
Homo sapiens
-
IC50: 0.0016 mM, vs. 10 nM estradiol
0.02
2-chlorophenol
Homo sapiens
-
IC50: 0.02 mM, vs. 10 nM estradiol
0.0052
2-phenylphenol
Homo sapiens
-
IC50: 0.0052 mM, vs. 10 nM estradiol
0.016
3',4'-dihydroxy-2,3-dichlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.096
3',4'-dihydroxy-4-monochlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.013
3,4-dichlorophenol
Homo sapiens
-
IC50: 0.013 mM, vs. 10 nM estradiol
0.017
3,5-dichlorophenol
Homo sapiens
-
IC50: 0.017 mM, vs. 10 nM estradiol
0.13
3-chlorophenol
Homo sapiens
-
IC50: 0.13 mM, vs. 10 nM estradiol
0.0025
4'-hydroxy-2',3,4-trichlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.0008
4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.0044
4'-hydroxy-2,3',4-trichlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.0098
4'-hydroxy-2,3'-dichlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.0039 - 0.0075
4'-hydroxy-2,5-dichlorobiphenyl
0.016
4'-hydroxy-3,4-dichlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.028
4'-hydroxy-4-monochlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.024
4-chloro-3-methylphenol
Homo sapiens
-
IC50: 0.024 mM, vs. 10 nM estradiol
0.11
4-Chlorophenol
Homo sapiens
-
IC50: 0.11 mM, vs. 10 nM estradiol
0.0006
4-hydroxy-2',3,5-trichlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.01
4-hydroxy-2,4'-dichlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.0044
4-hydroxy-3,3',5-trichlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.014
4-hydroxy-3,3'-dichlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.013
4-hydroxy-3,5-dichlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.01 - 0.0167
4-hydroxytamoxifen
0.1642 - 0.2536
5,7-dichlorokynurenic acid
0.016
6'-hydroxy-3,3'-4-trichlorobiphenyl
Homo sapiens
-
in 0.25 M potassium phosphate at pH 7.0, and 7.5 mM 2-mercaptoethanol, at 37C
0.008
daidzein
Danio rerio
Q7ZUS4
IC50: 0.008 mM, competitive
0.0017 - 0.0027
endoxifen
0.0012 - 0.0017
gavestinel
0.0025
genistein
Danio rerio
Q7ZUS4
IC50: 0.0025 mM, competitive
0.0337 - 9
Kynurenic acid
0.0473 - 0.0536
L689,560
0.005
Mefenamic acid
Homo sapiens
-
IC50 above 0.005 mM, isoform SULT1B1, using L-triiodothyronine as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37C; IC50 above 0.005 mM, isoform SULT1B1, using xanthurenic acid as substrate, in 50 mM Tris/HCl buffer, pH 7.4, 1 mM dithiothreitol, 5 mM MgCl2, and 1 mg/ml bovine serum albumin, at 37C
0.0083 - 0.015
N-demethyltamoxifen
0.0007
quercetin
Danio rerio
Q7ZUS4
IC50: 0.0007 mM, competitive
0.0111 - 0.0161
tamoxifen N-oxide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000831
-
-
0.000978
-
-
0.0041
activity with caffeic acid
0.0046
activity with catechin
0.0059
activity with chlorogenic acid
0.0066
activity with p-nitrophenol
0.0073
-
-
0.0108
activity with butylated hydroxy anisole
0.0154
activity with daidzein
0.0165
activity with kaempferol
0.0175
0.0184
activity with genistein
0.0191
activity with 17beta-estradiol
0.0201
-
-
0.0265
activity with myricetin
0.027
-
purified recombinant SULT1A2, substrate 4-hydroxy-2',3,5-trichlorobiphenyl
0.0283
activity with epigallocatechin gallate
0.0302
activity with estrone
0.0345
activity with n-propyl gallate
0.038
activity with beta-naphthol
0.045
-
full-length enzyme, using cholesterol as substrate, at pH 7.5 and 37C
0.0805
reaction with estrone
0.0916
reaction with 17beta-estradiol
0.133
-
-
0.9599
-
full-length enzyme, using pregnenolone as substrate, at pH 7.5 and 37C
0.9993
-
full-length enzyme, using dehydroepiandrosterone as substrate, at pH 7.5 and 37C
additional information
-
determination of 3alpha-sulfated bile acids
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6
5.5
-
substrate estradiol
6 - 7
-
assay at
6.5 - 7
-
3betahydroxysteroid activity
6.5
2 optima: pH 6.5 and pH 10-10.5, activity with estrone and n-propyl gallate
7
-
assay at
7 - 8
-
biphasic pH optimum, activity for estradiol, but not estrone
7.4
assay at
7.5
-
assay at
10 - 10.5
2 optima: pH 6.5 and pH 10-10.5, activity with estrone and n-propyl gallate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9.3
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 45
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
aortic smooth muscle cell line CRL-1999
Manually annotated by BRENDA team
-
Ishikawa adenocarcinoma
Manually annotated by BRENDA team
-
foetal tissue is a much richer source of enzyme than adult
Manually annotated by BRENDA team
-
aortic smooth muscle cell line CRL-1999
Manually annotated by BRENDA team
-
highest expression
Manually annotated by BRENDA team
SULT2A1
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
-
immunoblot analysis, antibodies against rat liver sulfotransferases
33785
-
x * 33785, calculated from amino acid sequence
34500
-
2 * 34500, SDS-PAGE
35000
-
2 * 35000, amino acid analysis; 2 * 35000, SDS-PAGE
37600
-
1 * 37600, recombinant protein, SDS-PAGE
38400
-
x * 38400, SDS-PAGE
39620
isoform SULT2B1a
41000
-
x * 41000, SDS-PAGE
41300
isoform SULT2B1b
60000 - 70000
-
gel filtration
61500
-
gel filtration
62000
x * 62000, SDS-PAGE
65000
-
sedimentation velocity experiment in preparative ultracentrifuge
68000 - 70000
-
gel filtration
68000
-
sucrose density gradient ultracentrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
-
isozyme SULT2B1b has an extension at the proline- and serine-rich carboxyl end of about 53 amino acids
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
the full-length enzyme is phosphorylated by casein kinase or Cdc2 protein kinase in vitro; the the proline- and serine-rich carboxyl end of SULT2B1 is phosphorylated at serine residues, e.g. by casein kinase or Cdc2 protein kinase in vitro
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion technique, structure of human dehydroepiandrosterone adenosine sulfotransferase in complex with androsterone has been solved at 2.7 A resolution
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42 - 45
-
truncated SULT2B1b is more sensitive to thermal inactivation than the full-length enzyme. After incubation at 42C for 30 min, dehydroepiandrosterone sulfation activity with truncated SULT2B1b is decreased 3.5fold more than that of full-length enzyme. After 45 min of incubation at 42C, truncated SULT2B1b activity is completely eliminated, whereas full-length SULT2B1b still maintains about 70% of its original activity
42
-
after 30 min, dehydroepiandrosterone sulfation activity with recombinant truncated SULT2B1b is decreased 3.5fold more than that of recombinant full-length SULT2B1b, after 45 min,the truncated SULT2B1b activity is completely eliminated, whereas full-length SULT2B1b still maintains about 70% of its original activity
additional information
-
the proline- and serine-rich carboxyl end is an important site in the immunogenicity, nuclear translocation, kinetic activity, and thermostability of SULT2B1b
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
marked instability, addition of 20% glycerol improves stability
-
removal of the extension at the proline- and serine-rich carboxyl end of about 53 amino acids significantly decreases the thermostability of the expressed enzyme as well as decreasing the rate of dehydroepiandrosterone sulfation
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, partially purified enzyme, stable for at least 3 months
-
-20C, storage for up to 2 months results in no decrease in activity
-
-70C, stored in glass ampoules , 0.05 M Tris-HCl buffer, pH 7.5, 0.1 mM DTT, 2% propylene glycol, 1 mg/ml protein, no significant difference in activity observed after storage for 3.5 months
-
0C, redialyzed againstTris/thiol buffer, stored under N2, activity retains for at least 4 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DE-52 column chromatography and hydroxyapatite column chromatography
-
DE52 anion exchange column chromatography and hydroxyapatite column chromatography
-
male-specific enzyme
-
Ni-NTA agarose column chromatography
Ni-NTA column chromatography
-
Ni-NTA column chromatography and Protein A-Sepharose column chromatography; recombinant His-tagged full-length SULT2B1b as well as truncated SULT2B1b without the PSC extension from Escherichia coli strain DH5alpha by nickel affinity chromatography
-
partial
recombinant enzyme, expressed in Escherichia coli
recombinant His-tagged SULT2A1 from Escherichia coli by nickel affinity chromatography, expression analysis
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3 isoenzymes cloned and expressed in Escherichia coli
-
3alphaHST and 3betaHST cDNA cloned and overexpressed in Escherichia coli
-
baculovirus expression-system, expression in Sf9 cells
-
cDNA for isozymes STS and EST cloned
-
cloning of 5'-flanking region of SULT2A1 and expression of a SULT2A1-reporter construct in Caco-2 cells, transcriptional regulation mechanism analysis, co-expression of human nuclear receptors constitutive androstane receptor CAR and retinoid X receptor alpha RXRalpha which are involved in the regulation, electrophoretic mobility shift assay for interaction study, overview
-
DHEA-ST cDNA cloned and sequenced from human adrenal cDNA library, expression vectors transfected into 293 cells, gene hEST, Hugo nomenclature named it STM gene, because it also codes for monoamine-sulfating phenolsulfotransferase M-PST
-
enzyme expression induction by 1alpha,25-dihydroxyvitamin D3 via the vitamin D responsive element C/ERB-alpha, but not C/ERB-beta, coactivators p300, steroid receptor coactivator 1 and 2, SRC-1 and SRC-2, but not SRC-3, are also required, regulation of transcription and translation, overview, co-expression of SULT2A1 with C/ERB-alpha and 1alpha,25-dihydroxyvitamin D3 in murine NIH3T3 cells
-
expressed in Escherichia coli BL21 using the pGEX-2TK expression system
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli strain M15
-
expressed in livers from Rattus norvegicus and Mus musculus by using a recombinant adenovirus
-
expressed in NIH/3T3 cells and in Escherichia coli BL21 cells
-
expression analysis of SULT2A1, induction of SULT2A1 expression by xenobiotic-activated nuclear receptors pregnane X receptor PXR, constitutive androstane receptor CAR, and the vitamin D3-activated nuclear receptor VDR, aging has no effect on the activity of an IR0 enhancer in the Sult2A1 chromatin to recruit VDR, RXR-alpha, and PXR in mice injected with D3 or PCN
-
expression and expression analysis of wild-type and mutant SULT2A1
-
expression in Escherichia coli
expression of His-tagged full-length SULT2B1b and truncated SULT2B1b without the PSC extension in Escherichia coli strain DH5alpha and in human BeWo choriocarcinoma cells, translocation of the wild-type enzyme to nuclei, but not of the truncation mutant; full-length isoform SULT2B1b as well as truncated SULT2B1b without the PS proline- and serine-rich carboxyl extension are expressed in Escherichia coli XL1-Blue cells
-
expression of His-tagged SULT2A in Escherichia coli
-
expression of SULT1A2 in Escherichia coli strain BL21 (DE3)
-
expression of SULT2B1b eliminates the cytotoxic effect of 7-oxo-cholesterol on 293T cells, which are normally devoid of SULT2B1b
-
gene SULT2A1, expression and regulation analysis, the enzyme promoter contains composite elements inducible by nuclear receptors pregnane X receptor, PXR, and constitutive androstane receptor, CAR, synergizing effects by HNF4alpha binding at a site near the promoter, overview
-
genomic library constructed in pTZ18R, expression of recombinant BNST3 in Escherichia coli
-
hEST1 and hEST2 cloned and overexpressed in Escherichia coli in fusion with GST
-
SULT2A1 is a target gene for metabolic regulation/induction by thyroid hormone T3, T3 overexpressing Hep-G2 cells, with co-expression of the T3-receptor-alpha, show increased SULT2A1 expression in a DNA microarray analysis, 5fold at protein level and 9fold at mRNA level, which is inhibited by cycloheximide indicating an indirect regulation mechanism via the transcription factor steroidogenic factor 1, SF1, SULT2A1 contains seven SF1 binding sites, analysis of promoter activity in wild-type and truncation mutants of the enzyme, overview
-
SULT2A1, determination of the promoter sequence, SULT2A1-reporter gene expression in Hep-G2 cells and expression analysis, the constitutive active receptor, CAR, mediates the methotrexate induction of SULT2A1 in both Caco-2 andHep G2 cells, vitamin D receptor, VDR, also upregulates SULT2A1 gene expression, while human pregnane X receptor, PXR, downregulates it
-
SULT2A1, DNA and amino acid sequence determination and analysis, relationship between SULT2A1 expression and sulfoconjugation of androstenone, quantitative expression analysis, overview, identification of nuclear receptors involved in regulating SULT2A1
SULT2B1 gene encodes 2 isoforms, SULT2B1a and SULT2B1b, generated by alternate splicing of the first exon, cloned and expressed in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
a 2 week's ethanol ingestion up-regulates hydroxysteroid sulfotransferase SULT2A1 in male rat liver and intestine
-
activation of liver X receptor alpha as well as liver X receptor synthetic ligands like TO901317 and GW3965 induce the expression of isoform SULT2A1 at mRNA, protein, and enzymatic levels
-
down-regulation of RORalpha and/or RORgamma by small interfering RNA inhibits the expression of endogenous SULT2A1
-
estrogen-related receptor alpha upregulates SULT2A1 transcription in Caco-2 cells
-
highest expression level is detected in epidermis from 3-day-old mice and then decreases before maturation
-
inhibition of SULT2B1b expression induces cell-cycle arrest and apoptosis in Hepa1-6 cells by upregulating the expression of FAS, downregulating the expression of cyclinB1, BCL2 and MYC in vitro and in vivo at both the transcript and protein levels. Knock-down of SULT2B1b expression significantly suppresses tumor growth in nude mouse xenografts
-
retinoid-related orphan receptor (ROR)alpha and RORgamma transactivate the SULT2A1 gene promoter through their binding to a ROR response element found in the SULT2A1 gene promoter
-
SULT2B1 is comparatively higher in the human hepatocarcinoma tumorous tissues than their adjacent tissues
-
the amount of SULT2B1b mRNA in human endometrial tissues is significantly higher during the midluteal phase than during other phases of the menstrual cycle. Expression of SULT2B1b mRNA is induced by cAMP or phosphate in human endometrial stromal cells, whereas it is induced by cAMP or relaxin in endometrial epithelial cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A679G
-
naturally occuring polymorphisms of SULT2A1, genotyping, SULT2A1 variant allele frequencies in African-American populations, overview
G187C
-
naturally occuring polymorphisms of SULT2A1, genotyping, SULT2A1 variant allele frequencies in African-American populations, overview
G781A
-
naturally occuring polymorphisms of SULT2A1, genotyping, SULT2A1 variant allele frequencies in African-American populations, overview
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine