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Literature summary for 2.8.2.15 extracted from

  • Gower, D.B.; Cooke, G.M.; Ferguson, S.E.
    Sulphoconjugation of steroids in porcine liver. Partial purification of the cytosolic sulphotransferases for pregnenolone and 5alpha-androst-16-en-3beta-ol (1982), FEBS Lett., 150, 507-510.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Sus scrofa 5737
-
cytosol
-
Sus scrofa 5829
-

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
pig
-

Purification (Commentary)

Purification (Comment) Organism
partial Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, storage for up to 2 months results in no decrease in activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 5alpha-androst-16-en-3beta-ol
-
Sus scrofa adenosine 3',5'-bisphosphate + ?
-
r
3'-phosphoadenylylsulfate + pregnenolone
-
Sus scrofa adenosine 3',5'-bisphosphate + pregnenolone sulfate pregn-5-en-20-one 3-sulfate r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45
-
Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.3
-
Sus scrofa