Cloned (Comment) | Organism |
---|---|
3alphaHST and 3betaHST cDNA cloned and overexpressed in Escherichia coli | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000125 | - |
17beta-estradiol | pH 7.7, 37°C, 3-alphaHST isoform | Cavia porcellus | |
0.00019 | - |
allopregnanolone | pH 7.7, 37°C, 3-alphaHST isoform | Cavia porcellus | |
0.0002 | - |
testosterone | pH 7.7, 37°C, 3-alphaHST isoform | Cavia porcellus | |
0.00025 | - |
pregnenolone | pH 7.7, 37°C, 3-betaHST isoform | Cavia porcellus | |
0.00026 | - |
androsterone | pH 7.7, 37°C, 3-alphaHST isoform | Cavia porcellus | |
0.000788 | - |
dehydroepiandrosterone | pH 7.7, 37°C, 3-betaHST isoform | Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Cavia porcellus | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + dehydroepiandrosterone | Cavia porcellus | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
guinea pig | - |
Purification (Comment) | Organism |
---|---|
TST | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Cavia porcellus | - |
liver | - |
Cavia porcellus | - |
additional information | both 3alphaHST and 3betaHST activities are essentially undetectable in the testis | Cavia porcellus | - |
prostate | small but detectable amount of 3alphaHST activity | Cavia porcellus | - |
small intestine | - |
Cavia porcellus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylylsulfate + 17beta-estradiol | 3alphaHST isoform | Cavia porcellus | adenosine 3',5'-bisphosphate + ? | - |
r | |
3'-phosphoadenylylsulfate + 3alpha-hydroxy-5alpha-androstan-17-one | 3alphaHST isoform | Cavia porcellus | adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + allopregnanolone | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + allopregnanolone sulfate | - |
? | |
3'-phosphoadenylylsulfate + allopregnenolone | 3alphaHST isoform | Cavia porcellus | adenosine 3',5'-bisphosphate + allopregnenolone sulfate | - |
r | |
3'-phosphoadenylylsulfate + dehydroepiandrosterone | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + dehydroepiandrosterone | 3betaHST isoform | Cavia porcellus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
3'-phosphoadenylylsulfate + pregnenolone | 3betaHST isoform | Cavia porcellus | adenosine 3',5'-bisphosphate + pregnenolone sulfate | - |
r | |
3'-phosphoadenylylsulfate + testosterone | 3alphaHST isoform | Cavia porcellus | adenosine 3',5'-bisphosphate + testosterone sulfate | only 3alpha-hydroxysteroid isoform, 3beta-hydroxysteroid isoform exhibits no TST activity | r | |
additional information | estrone is no substrate | Cavia porcellus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3alpha-hydroxysteroid sulfotransferase | - |
Cavia porcellus |
testosterone sulfotransferase | - |
Cavia porcellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | activity with 3alpha-hydroxysteroid | Cavia porcellus |
6.5 | 7 | 3betahydroxysteroid activity | Cavia porcellus |