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Literature summary for 2.8.2.15 extracted from

  • Park, B.C.; Lee, Y.C.; Strott, C.A.
    Testosterone sulfotransferase: Evidence in the guinea pig that this reaction is carried out by 3alpha-hydroxysteroid sulfotransferase (1999), Steroids, 64, 510-517.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
3alphaHST and 3betaHST cDNA cloned and overexpressed in Escherichia coli Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000125
-
17beta-estradiol pH 7.7, 37°C, 3-alphaHST isoform Cavia porcellus
0.00019
-
allopregnanolone pH 7.7, 37°C, 3-alphaHST isoform Cavia porcellus
0.0002
-
testosterone pH 7.7, 37°C, 3-alphaHST isoform Cavia porcellus
0.00025
-
pregnenolone pH 7.7, 37°C, 3-betaHST isoform Cavia porcellus
0.00026
-
androsterone pH 7.7, 37°C, 3-alphaHST isoform Cavia porcellus
0.000788
-
dehydroepiandrosterone pH 7.7, 37°C, 3-betaHST isoform Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Cavia porcellus 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + dehydroepiandrosterone Cavia porcellus
-
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
r

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
guinea pig
-

Purification (Commentary)

Purification (Comment) Organism
TST Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland
-
Cavia porcellus
-
liver
-
Cavia porcellus
-
additional information both 3alphaHST and 3betaHST activities are essentially undetectable in the testis Cavia porcellus
-
prostate small but detectable amount of 3alphaHST activity Cavia porcellus
-
small intestine
-
Cavia porcellus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + 17beta-estradiol 3alphaHST isoform Cavia porcellus adenosine 3',5'-bisphosphate + ?
-
r
3'-phosphoadenylylsulfate + 3alpha-hydroxy-5alpha-androstan-17-one 3alphaHST isoform Cavia porcellus adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
-
r
3'-phosphoadenylylsulfate + allopregnanolone
-
Cavia porcellus adenosine 3',5'-bisphosphate + allopregnanolone sulfate
-
?
3'-phosphoadenylylsulfate + allopregnenolone 3alphaHST isoform Cavia porcellus adenosine 3',5'-bisphosphate + allopregnenolone sulfate
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
Cavia porcellus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone 3betaHST isoform Cavia porcellus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
r
3'-phosphoadenylylsulfate + pregnenolone 3betaHST isoform Cavia porcellus adenosine 3',5'-bisphosphate + pregnenolone sulfate
-
r
3'-phosphoadenylylsulfate + testosterone 3alphaHST isoform Cavia porcellus adenosine 3',5'-bisphosphate + testosterone sulfate only 3alpha-hydroxysteroid isoform, 3beta-hydroxysteroid isoform exhibits no TST activity r
additional information estrone is no substrate Cavia porcellus ?
-
?

Synonyms

Synonyms Comment Organism
3alpha-hydroxysteroid sulfotransferase
-
Cavia porcellus
testosterone sulfotransferase
-
Cavia porcellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 activity with 3alpha-hydroxysteroid Cavia porcellus
6.5 7 3betahydroxysteroid activity Cavia porcellus