Information on EC 3.2.1.114 - mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.114
-
RECOMMENDED NAME
GeneOntology No.
mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
hydrolysis of N-glycosyl bond
-
-
hydrolysis of O-glycosyl bond
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
mannosyl-glycoprotein N-acetylglucosaminyltransferases
-
-
Metabolic pathways
-
-
N-Glycan biosynthesis
-
-
Various types of N-glycan biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
(1->3)-(1->6)-mannosyl-oligosaccharide alpha-D-mannohydrolase
Involved in the synthesis of glycoproteins.
CAS REGISTRY NUMBER
COMMENTARY hide
349553-33-9
alpha-mannosidase III
82047-77-6
alpha-mannosidase II
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
strain YJ-407
UniProt
Manually annotated by BRENDA team
strain YJ-407
UniProt
Manually annotated by BRENDA team
strain Bm-N
-
-
Manually annotated by BRENDA team
strain Bm-N
-
-
Manually annotated by BRENDA team
strain Mb-0503
-
-
Manually annotated by BRENDA team
strain Mb-0503
-
-
Manually annotated by BRENDA team
Neosartorya fischeri
-
-
-
Manually annotated by BRENDA team
plant
-
-
-
Manually annotated by BRENDA team
Sf-21
-
-
Manually annotated by BRENDA team
gene M1 GAS SF370
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Man)3GlcNAc + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
(Man)6GlcNAc + H2O
?
show the reaction diagram
-
rate at 2% of GlcNAc(Man)5GlcNAc hydrolysis
-
-
?
(Man)7GlcNAc + H2O
?
show the reaction diagram
-
rate at 3% of GlcNAc(Man)5GlcNAc hydrolysis
-
-
?
(Man)8GlcNAc + H2O
?
show the reaction diagram
-
rate at 5% of GlcNAc(Man)5GlcNAc hydrolysis
-
-
?
(Man)9GlcNAc + H2O
(Man)5GlcNAc + D-mannose
show the reaction diagram
-
hydrolysis to (Man)5GlcNAc in the presence of Fe2+, Co2+, and Mn2+. Without activating cations the main reaction product is (Man)8GlcNAc
-
-
?
(Man)9GlcNAc + H2O
?
show the reaction diagram
-
rate at 6.4% of GlcNAc(Man)5GlcNAc hydrolysis
-
-
?
2,4-dinitrophenyl alpha-D-mannopyranoside + H2O
2,4-dinitrophenol + alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
2,4-dinitrophenyl alpha-D-mannopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
2,4-dinitrophenyl alpha-D-mannoside + H2O
2,4-dinitrophenol + alpha-D-mannose
show the reaction diagram
2,4-dinitrophenyl-alpha-D-mannopyranoside + H2O
2,4-dinitrophenol + D-mannose
show the reaction diagram
-
-
-
-
?
2,5-dinitrophenyl-alpha-D-mannopyranoside + H2O
2,4-dinitrophenol + D-mannose
show the reaction diagram
-
-
-
?
2,5-dinitrophenyl-alpha-D-mannopyranoside + H2O
2,5-dinitrophenol + alpha-D-mannose
show the reaction diagram
pH 5.6, room temperature
-
-
?
2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->3)-[alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->6)]-beta-D-mannopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-alpha-D-glucopyranose + H2O
2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->3)-[alpha-D-mannopyranosyl-(1->6)]-beta-D-mannopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-alpha-D-glucopyranose + D-mannose
show the reaction diagram
-
-
-
-
ir
2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->3)-[alpha-D-mannopyranosyl-(1->3)-[alpha-D-mannopyranosyl-(1->6)]-alpha-D-mannopyranosyl-(1->6)]-beta-D-mannopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-alpha-D-glucopyranose + H2O
2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->3)-[alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->6)]-beta-D-mannopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-alpha-D-glucopyranose + D-mannose
show the reaction diagram
-
-
-
-
ir
2-deoxy-2-fluoro-alpha-D-mannosyl fluoride
?
show the reaction diagram
-
wild-type and D341N mutant GMII, acts as very slow substrate of the mutant enzyme with a rate-limiting deglycosylation step
-
-
?
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O
2 alpha-D-mannopyranose
show the reaction diagram
-
Bt3991 displays particularly high activity against alpha-1,3-mannobiose
-
-
?
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O
alpha-D-mannopyranose
show the reaction diagram
4-methyl-umbelliferyl-alpha-D-mannopyranoside + H2O
4-methyl-umbelliferone + alpha-D-mannopyranose
show the reaction diagram
-
30 min, 37C
-
-
?
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
4-methylumbelliferol + alpha-D-mannopyranose
show the reaction diagram
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-mannoside + H2O
4-methylumbelliferol + alpha-D-mannose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-mannoside + H2O
4-methylumbelliferone + alpha-D-mannose
show the reaction diagram
4-nitrophenyl alpha-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannopyranose
show the reaction diagram
4-nitrophenyl alpha-D-mannoside + H2O
4-nitrophenol + alpha-D-mannose
show the reaction diagram
4-nitrophenyl-alpha-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannopyranose
show the reaction diagram
-
30 min, 37C
-
-
?
4-nitrophenyl-alpha-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannose
show the reaction diagram
30 min, 20C
-
-
?
4-nitrophenyl-alpha-D-mannoside + H2O
4-nitrophenol + alpha-D-mannose
show the reaction diagram
-
-
-
-
?
5-fluoro-beta-L-gulosyl fluoride + H2O
?
show the reaction diagram
-
acts as slow substrate with a rate-limiting deglycosylation step, wild-type and D341N mutant GMII
-
-
?
alpha-D-Man-(1,6)-D-Man + H2O
alpha-D-mannopyranose
show the reaction diagram
-
-
-
-
?
alpha-D-Manp-(1,3)-D-Manp + H2O
alpha-D-mannopyranose
show the reaction diagram
-
-
-
-
?
alpha-D-Manp-(1,6)-D-Manp + H2O
alpha-D-mannopyranose
show the reaction diagram
-
-
-
-
?
baker's yeast mannan + H2O
?
show the reaction diagram
-
-
-
-
?
D-Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
-
NaBH4 reduced, poor substrate
-
-
?
D-Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
D-Manbeta(1-4)GlcNAc + alpha-D-mannose
show the reaction diagram
D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
show the reaction diagram
D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O
D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + alpha-D-mannose
show the reaction diagram
-
i.e. glycopeptide III, derived from ovalbumin, no hydrolysis of the innermost alpha-1,6-linked or the alpha-1,2-linked mannose
i.e. glycopeptide II
?
D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
D-Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
show the reaction diagram
-
NaBH4 reduced, at lower rate than Manalpha(1-3)Manbeta(1-4)GlcNAc hydrolysis
NaBH4 reduced
?
GlcNAc(Man)5GlcNAc + 2 H2O
GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose
show the reaction diagram
GlcNAc(Man)5GlcNAc2 + H2O
GlcNAc(Man)3GlcNAc2 + D-mannose
show the reaction diagram
GlcNAcbeta(1-2)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
-
NaBH4 reduced, hydrolysis at equal rate as Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, galactosyl and sialyl-galactosyl derivatives are substrates, too
-
-
?
GlcNAcbeta(1-6)(GlcNAcbeta(1-2))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
-
NaBH4 reduced, rate at 50% of Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc hydrolysis
-
-
?
GlcNAcMan5GlcNAc2 + H2O
?
show the reaction diagram
-
-
-
-
?
GlcNAcMan5GlcNAc2 + H2O
? + alpha-D-mannose
show the reaction diagram
-
-
-
-
?
high mannose N-glycan + H2O
?
show the reaction diagram
-
-
-
?
Man5GlcNAc2 + H2O
Man3GlcNAc2 + 2 alpha-D-mannopyranose
show the reaction diagram
Manalpha(1-3)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))(Xylbeta(1-4))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + H2O
Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))(Xylbeta(1-4))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + alpha-D-mannose
show the reaction diagram
-
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + H2O
Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + alpha-D-mannose
show the reaction diagram
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
show the reaction diagram
-
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O
Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
show the reaction diagram
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-6)(Manalpha(1-3))D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
show the reaction diagram
-
NaBH4 reduced
NaBH4 reduced
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
Manalpha(1-3)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
show the reaction diagram
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose
show the reaction diagram
-
-
-
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O
Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
show the reaction diagram
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O
Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + alpha-D-mannose
show the reaction diagram
-
processing of asparagine-linked oligosaccharides by alpha-D-mannosidase dependent on the prior action of UDP-GlcNAc:alpha-D-mannoside beta2-N-acetylglucosaminyltransferase I, removes 2 mannosyl residues, via GlcNAc(Man)4(GlcNAc)2Asn
-
?
ovalbumin + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
p-nitrophenol + alpha-D-mannopyranose
show the reaction diagram
-
-
-
-
?
pyridylaminated (Man)5(GlcNAc)2 + H2O
pyridylaminated (Man)3(GlcNAc)2 + alpha-D-mannose
show the reaction diagram
pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
show the reaction diagram
R-Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
?
show the reaction diagram
-
-
-
-
?
yeast mannan + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O
alpha-D-mannopyranose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
GlcNAc(Man)5GlcNAc + 2 H2O
GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose
show the reaction diagram
GlcNAcMan5GlcNAc2 + H2O
? + alpha-D-mannose
show the reaction diagram
-
-
-
-
?
high mannose N-glycan + H2O
?
show the reaction diagram
Q99YP5
-
-
-
?
Man5GlcNAc2 + H2O
Man3GlcNAc2 + 2 alpha-D-mannopyranose
show the reaction diagram
-
-
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + H2O
Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + alpha-D-mannose
show the reaction diagram
Q9LFR0
physiological substrate, conversion by sequential removal of two alpha1,6-linked and alpha1,3-linked mannose residues from the alpha-1,6-branch of the substrate
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O
Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
show the reaction diagram
-
alpha-mannosidase IIx, M6Gn2 is the primary target in vivo, integral part of N-glycan biosynthesis
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O
Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
preferred divalent cation
Ni2+
-
alpha-mannosidase III, little activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2R,3R,4R,5S)-1-(methylthio)-2,3,4-trihydroxy-5-(N-(p-allyl-ester-benzyl)amino)cyclopentane
-
-
(1R,2R,3R,4R,5S)-1-(methylthio)-2,3,4-trihydroxy-5-(N-(p-bromo-benzyl)amino)cyclopentane
-
-
(1R,2R,3R,4R,5S)-1-(methylthio)-2,3,4-trihydroxy-5-(N-(p-chloro-benzyl)amino)cyclopentane
-
-
(1R,2R,3R,4R,5S)-1-(methylthio)-2,3,4-trihydroxy-5-(N-(p-fluoro-benzyl)amino)cyclopentane
-
-
(1R,2R,3R,4R,5S)-1-(methylthio)-2,3,4-trihydroxy-5-(N-(p-methoxy-benzyl)amino)cyclopentane
-
-
(1R,2R,3R,4R,5S)-1-(methylthio)-2,3,4-trihydroxy-5-(N-benzylamino)cyclopentane
-
-
(1R,2R,3R,4S,5R)-4-amino-5-methoxycyclopentane-1,2,3-triol
-
;
(1R,2R,3R,4S,5S)-1,2,3,4-tetrahydroxy-5-(N-(p-allyl ester benzyl)amino)cyclopentane
-
-
(1R,2R,3R,4S,5S)-1,2,3,4-tetrahydroxy-5-(N-(p-bromo-benzyl)amino)cyclopentane
-
-
(1R,2R,3R,4S,5S)-1,2,3,4-tetrahydroxy-5-(N-(p-chloro-benzyl)amino)cyclopentane
-
-
(1R,2R,3R,4S,5S)-1,2,3,4-tetrahydroxy-5-(N-(p-fluoro-benzyl)amino)cyclopentane
-
-
(1R,2R,3R,4S,5S)-1,2,3,4-tetrahydroxy-5-(N-(p-methoxy-benzyl)amino)cyclopentane
-
-
(1R,2R,3R,4S,5S)-1,2,3,4-tetrahydroxy-5-(N-benzylamino)cyclopentane
-
-
(1R,2R,3S,4R,5R)-5-aminocyclopentane-1,2,3,4-tetrol
-
-
(1R,2S,3R,4R,5R)-5-aminocyclopentane-1,2,3,4-tetraol
-
-
(1R,2S,8aS)-1,2-dihydroxyoctahydrothieno[1,2-a]thiopyranium chloride
-
good inhibitor, lacks a hydroxyl group at C-5, more than 140fold better inhibitor of GMII than di-epi-swainsonine
(1R,6R,7R,8S)-7,8-dihydroxy-5-thia-1-thioniabicyclo[4.3.0]nonane chloride
-
synthetic inhibitor, selective and potent inhibition at 1 mM, 97% inhibition of the activity of the liver lysosomal fraction at pH 4.0, 100% at pH 6.5
(1S,2R,5R,8R,8aR)-5-[2-(4-tert-butylphenyl)ethyl]octahydroindolizine-1,2,8-triol
-
-
(1S,2R,5S,8R,8aR)-5-[2-(4-tert-butylphenyl)ethyl]octahydroindolizine-1,2,8-triol
-
-
(1S,2R,6R,7R,8S,8aS)-octahydroindolizine-1,2,6,7,8-pentol
-
most active
(1S,2R,8R,8aR)-octahydroindolizine-1,2,8-triol
-
-
(2R,3R,4R,5R)-2-(hydroxymethyl)piperidine-3,4,5-triol
-
-
(2R,3R,4R,5R)-5-amino-2-(hydroxymethyl)piperidine-3,4-diol
-
-
(2R,3R,4S)-2-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidine-3,4-diol
-
-
(2R,3R,4S)-2-([[(1S)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidine-3,4-diol
-
-
(2R,3R,4S)-2-[[((1R)-2-hydroxy-1-[4'-(trifluoromethyl)-[1,1'-biphenyl]-4-yl]ethyl)amino] methyl]pyrrolidine-3,4-diol
-
inhibits the proliferation of primary cells and cell lines of different origins, irrespective of Bcl-2 expression levels, inducing a G2/Mcell cycle arrest and by modification of genes involved in cell cycle progression and survival, IC50 in vivo and cytotoxic effects in different cell lines, overview
(2R,3R,4S)-2[([(1R)-2-hydroxy-1-(4-methoxyphenyl)ethyl]amino)-methyl]pyrrolidine-3,4-diol
-
-
(2R,3R,4S)-2[[((1R)-1-[1,1'-biphenyl]-4-yl-2-hydroxyethyl)amino]-methyl]pyrrolidine-3,4-diol
-
-
(2R,3R,4S)-2[[((1R)-2-hydroxy-1-(4-(phenylmethoxy)phenyl)ethyl)amino]methyl]pyrrolidine-3,4-diol
-
-
(2R,3R,4S)-2[[((1R)-2-hydroxy-1-[4-(2-thienyl)phenyl]ethyl)-amino]methyl]pyrrolidine-3,4-diol
-
-
(2R,3R,4S)-2[[((1R)-2-hydroxy-1-[4-(prop-2-enyloxy)phenyl]-ethyl)amino]methyl] pyrrolidine-3,4-diol
-
-
(2R,3R,4S,5R)-2-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)-5-methylpyrrolidine-3,4-diol
-
-
(2R,3R,4S,5S)-6-amino-2-(hydroxymethyl)-2,3,4,5-tetrahydropyridine-3,4,5-triol
-
-
(2R,3S,4R)-2-[(1R)-1-hydroxyethyl]pyrrolidine-3,4-diol
-
most active
(2R,3S,4S)-1-[(2S,3S)-2,4-dihydroxy-3-(sulfooxy)butyl]-3,4-dihydroxy-2-(hydroxymethyl)tetrahydrothiophenium
-
-
(2S,3R,4S)-2-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidine-3,4-diol
(2S,3R,4S)-2-[([(1R)-2-[(4-bromophenyl)(hydroxy)methoxy]-1-phenylethyl]amino)methyl]pyrrolidine-3,4-diol
-
-
(3R,4R)-4-aminopyrrolidin-3-ol
-
-
(3R,4R,5R)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)-1-methylpyrrolidin-2-one
-
-
(3R,4R,5R)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidin-2-one
-
-
(3R,4R,5S)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)-1-methylpyrrolidin-2-one
(3R,4R,5S)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidin-2-one
(3R,4R,5S)-5-[([(1R)-2-[(4-bromophenyl)(hydroxy)methoxy]-1-phenylethyl]amino)methyl]-3,4-dihydroxy-1-methylpyrrolidin-2-one
-
-
(3R,4R,5S)-5-[([(1R)-2-[(4-bromophenyl)(hydroxy)methoxy]-1-phenylethyl]amino)methyl]-3,4-dihydroxypyrrolidin-2-one
-
-
(3S,4S,5R,6R,E)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-one O-4-chlorophenylcarbamoyl oxime
-
(5R,6R,7S,8R)-5-methyl-1,5,6,7,8,8a-hexahydrotetrazolo[1,5-a]pyridine-6,7,8-triol
-
-
(5R,6R,7S,8S)-5-(hydroxymethyl)-1,5,6,7,8,8a-hexahydroimidazo[1,2-a]pyridine-6,7,8-triol
-
-
(RS)-2-phenylethyl alpha-D-mannopyranosyl sulfoxide
-
-
-
1,4-Dideoxy-1,4-imino-D-mannitol
1-(4-methylphenyl)-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone
-
-
1-(4-tert-butylphenyl)-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone
-
-
1-(4-tert-butylphenyl)-2-[(1S,2R,5S,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone
-
-
1-deoxyamino-cyclopentitetrol
-
-
1-deoxyaminocyclopentitetrol
1-deoxymannojirimycin
1-phenyl-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone
-
-
2-deoxy-2-fluoro-alpha-D-mannosyl fluoride
-
reversible, D341N mutant GMII
2-phenylethyl alpha-D-mannopyranosyl sulfone
-
-
5-fluoro-beta-L-gulosyl fluoride
-
reversible, wild-type and D341N mutant GMII, inhibits only at low assay temperatures, acts as slow substrate at 37C
5-thio-alpha-D-mannopyranosylamine
-
-
8,8a-di-epi-swainsonine
-
-
8-epi-lentiginosine
-
-
8a-epi-swainsonine
-
-
alpha-1,6-linked trisaccharide of mannose
-
1.5 mM, 40% inhibition
-
benzyl alpha-D-mannopyranosyl sulfone
-
-
-
D-Mannonolactam amidrazone
deoxymannojirimycin
-
-
di-epi-swainsonine
-
weak inhibitor
diastereomer of salacinol
-
-
diastereomer of seleno-salacinol
-
-
Fe3+
-
less potent inhibitor than Fe2+ and Cu2+, 1 mM, 24-30% inhibition
gluco-hydroxyiminolactam
-
guanidinium hydrochloride
Neosartorya fischeri
-
the enzyme loses 54% and 70% of the original activity in 0.5 M and 1.0 M guanidinium hydrochloride, respectively. Irreversible denaturation at higher concentration of 6 M of guanidinium hydrochloride, kinetics, overview. The protein almost completely unfolds in 4.0 M guanidinium hydrochloride
hydroxyiminolactam
-
-
-
kifunensine
mannose
-
250 mM, 30% inhibition
mannostatin
Mannostatin A
mannostatin B
-
; strong inhibitor, reversible, competitive
meso-aminocyclopentitretrol
-
-
N-benzyl mannostatin A
-
structural basis of the inhibition of Golgi alpha-mannosidase II and the role of the thiomethyl moiety in ligand-protein interactions, overview
N-benzyl-mannostatin
-
-
N-octyl-6-epi-valienamine
-
N-[(R)-amino(phenyl)methyl]-5-thio-alpha-D-mannopyranosylamine
-
-
p-Chloromercuriphenylsulfonate
seleno-salacinol
-
-
swainsonine
[[(3S,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]amino] N-(4-chlorophenyl)carbamate
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
activation
ATP
-
1 mM, 27% activation
bovine serum albumin
-
0.125 mg/l, 35% activation
-
CMP
-
1 mM, 25% activation
dithiothreitol
-
activation
fucose
-
250 mM, 15-20% activation
Triton X-100
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05 - 5.5
2,4-dinitrophenyl alpha-D-mannoside
25
2-deoxy-2-fluoro-alpha-D-mannosyl fluoride
-
pH 5.6, 37C, above, wild-type GMII
9 - 27
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
3.6
4-methylumbelliferyl alpha-D-mannopyranoside
pH 6.8, 37C, recombinant enzyme
0.55 - 1.065
4-methylumbelliferyl-alpha-D-mannopyranoside
2.7
4-nitrophenyl alpha-D-mannopyranoside
-
pH 7.0, 37C, Bt3991
30 - 40
4-nitrophenyl alpha-D-mannoside
0.2
5-fluoro-beta-L-gulosyl fluoride
-
pH 5.6, 37C, wild-type GMII
14
alpha-D-Man-(1,6)-D-Man
-
-
0.2
D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn
-
i.e. glycopeptide III
1.25
Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.048 - 8.6
2,4-dinitrophenyl alpha-D-mannoside
4.67 - 6.4
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
0.03
4-methylumbelliferyl alpha-D-mannopyranoside
Streptococcus pyogenes
Q99YP5
pH 6.8, 37C, recombinant enzyme
0.0025
4-nitrophenyl alpha-D-mannopyranoside
Homo sapiens
-
pH 7.0, 37C, Bt3991
0.0051
5-fluoro-beta-L-gulosyl fluoride
Drosophila melanogaster
-
pH 5.6, 37C, wild-type GMII
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
2,4-dinitrophenyl alpha-D-mannopyranoside
Streptococcus pyogenes
Q99YP5
pH 6.8, 37C, recombinant enzyme
75937
0.24 - 0.52
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
0.0009
4-nitrophenyl alpha-D-mannopyranoside
Homo sapiens
-
pH 7.0, 37C, Bt3991
7621
54 - 351
4-nitrophenyl alpha-D-mannoside
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00322
(1R,2R,3R,4R,5S)-1-(methylthio)-2,3,4-trihydroxy-5-(N-(p-allyl ester benzyl)amino)cyclopentane
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.00051
(1R,2R,3R,4R,5S)-1-(methylthio)-2,3,4-trihydroxy-5-(N-(p-bromo-benzyl)amino)cyclopentane
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.00091
(1R,2R,3R,4R,5S)-1-(methylthio)-2,3,4-trihydroxy-5-(N-(p-chloro-benzyl)amino)cyclopentane
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.00053
(1R,2R,3R,4R,5S)-1-(methylthio)-2,3,4-trihydroxy-5-(N-(p-fluoro-benzyl)amino)cyclopentane
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.00052
(1R,2R,3R,4R,5S)-1-(methylthio)-2,3,4-trihydroxy-5-(N-(p-methoxy-benzyl)amino)cyclopentane
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.00088
(1R,2R,3R,4R,5S)-1-(methylthio)-2,3,4-trihydroxy-5-(N-benzylamino)cyclopentane
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.000076
(1R,2R,3R,4S,5R)-4-amino-5-methoxycyclopentane-1,2,3-triol
-
;
0.0044
(1R,2R,3R,4S,5S)-1,2,3,4-tetrahydroxy-5-(N-(p-allyl ester benzyl)amino)cyclopentane
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.0076
(1R,2R,3R,4S,5S)-1,2,3,4-tetrahydroxy-5-(N-(p-bromo-benzyl)amino)cyclopentane
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.0081
(1R,2R,3R,4S,5S)-1,2,3,4-tetrahydroxy-5-(N-(p-chloro-benzyl)amino)cyclopentane
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.006
(1R,2R,3R,4S,5S)-1,2,3,4-tetrahydroxy-5-(N-(p-fluoro-benzyl)amino)cyclopentane
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.0066
(1R,2R,3R,4S,5S)-1,2,3,4-tetrahydroxy-5-(N-(p-methoxy-benzyl)amino)cyclopentane
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.01
(1R,2R,3R,4S,5S)-1,2,3,4-tetrahydroxy-5-(N-benzylamino)cyclopentane
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.0003
(1R,2R,3S,4R,5R)-5-aminocyclopentane-1,2,3,4-tetrol
0.00002
(1S,2R,8R,8aR)-octahydroindolizine-1,2,8-triol
-
20C, pH 6.8
0.067
(2R,3R,4S)-2-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidine-3,4-diol
-
20C, pH 6.8
0.000135 - 0.067
(2S,3R,4S)-2-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidine-3,4-diol
0.001
(3R,4R,5R)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)-1-methylpyrrolidin-2-one
-
20C, pH 6.8
0.022
(3R,4R,5R)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidin-2-one
-
20C, pH 6.8
0.000206 - 0.0032
(3R,4R,5S)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)-1-methylpyrrolidin-2-one
0.004 - 0.022
(3R,4R,5S)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidin-2-one
0.52
(3S,4S,5R,6R,E)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-one O-4-chlorophenylcarbamoyl oxime
-
0.0027
1-(4-methylphenyl)-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone
0.265
1-deoxyamino-cyclopentitetrol
0.4 - 0.75
1-deoxymannojirimycin
0.0028
1-phenyl-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone
-
pH 5.75, 25C
7.5
2-deoxy-2-fluoro-alpha-D-mannosyl fluoride
-
pH 5.6, 37C, D341N mutant GMII
0.6
5-fluoro-beta-L-gulosyl fluoride
-
pH 5.6, 37C, D341N mutant GMII
0.02
8,8a-di-epi-swainsonine
-
liver lysosomal fraction
0.75
8a-epi-swainsonine
-
liver lysosomal fraction
0.4
deoxymannojirimycin
pH 5.6
0.07
gluco-hydroxyiminolactam
; pH 5.6
0.013
glucoimidazole
; pH 5.6
5 - 5.2
kifunensine
0.002
mannoimidazole
; pH 5.6
0.00021
mannostatin
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.000036
Mannostatin A
0.00015
mannostatin B
-
;
50
meso-aminocyclopentitretrol
-
reaction volume containing 4-methylumbelliferyl alpha-D-mannopyranoside, sodium acetate pH 5.6 and ZnCl at 37C
0.000249
N-benzyl mannostatin A
0.017
N-octyl-6-epi-valienamine
; pH 5.6
0.02
noeuromycin
; pH 5.6
0.0000105 - 0.018
swainsonine
0.52
[[(3S,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]amino] N-(4-chlorophenyl)carbamate
pH 5.6
additional information
additional information
-
although too weak for full Ki analyses with the amounts of material available, all analogues with salacinol-like stereochemistry at positions 2 and 3 proved to be weak inhibitors of the enzyme with IC50 values of approximately 7.5 mM
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00025
(1S,2R,5R,8R,8aR)-5-[2-(4-tert-butylphenyl)ethyl]octahydroindolizine-1,2,8-triol
Drosophila melanogaster
-
pH 5.75, 25C
0.000044
(1S,2R,5S,8R,8aR)-5-[2-(4-tert-butylphenyl)ethyl]octahydroindolizine-1,2,8-triol
Drosophila melanogaster
-
pH 5.75, 25C
0.8
(2R,3R,4S)-2-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidine-3,4-diol
Drosophila melanogaster
-
-
0.72
(2R,3R,4S)-2-([[(1S)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidine-3,4-diol
Drosophila melanogaster
-
-
1
(2R,3R,4S,5R)-2-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)-5-methylpyrrolidine-3,4-diol
Drosophila melanogaster
-
-
0.025 - 0.05
(2S,3R,4S)-2-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidine-3,4-diol
0.075 - 0.2
(2S,3R,4S)-2-[([(1R)-2-[(4-bromophenyl)(hydroxy)methoxy]-1-phenylethyl]amino)methyl]pyrrolidine-3,4-diol
0.0005 - 0.002
(3R,4R,5S)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)-1-methylpyrrolidin-2-one
0.05 - 0.35
(3R,4R,5S)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidin-2-one
0.00075 - 0.075
(3R,4R,5S)-5-[([(1R)-2-[(4-bromophenyl)(hydroxy)methoxy]-1-phenylethyl]amino)methyl]-3,4-dihydroxy-1-methylpyrrolidin-2-one
0.075 - 0.75
(3R,4R,5S)-5-[([(1R)-2-[(4-bromophenyl)(hydroxy)methoxy]-1-phenylethyl]amino)methyl]-3,4-dihydroxypyrrolidin-2-one
2.5
(RS)-2-phenylethyl alpha-D-mannopyranosyl sulfoxide
Homo sapiens
-
in 50 mM sodium acetate buffer pH 5.8, at 37C
-
0.000029
1-(4-methylphenyl)-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone
0.00025
1-(4-tert-butylphenyl)-2-[(1S,2R,5S,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone
Drosophila melanogaster
-
pH 5.75, 25C
0.00003
1-phenyl-2-[(1S,2R,5R,8R,8aR)-1,2,8-trihydroxyoctahydroindolizin-5-yl]ethanone
Drosophila melanogaster
-
pH 5.75, 25C
1.5
2-phenylethyl alpha-D-mannopyranosyl sulfone
Homo sapiens
-
in 50 mM sodium acetate buffer pH 5.8, at 37C
0.014
8-epi-lentiginosine
Drosophila melanogaster
-
-
2
benzyl alpha-D-mannopyranosyl sulfone
Homo sapiens
-
in 50 mM sodium acetate buffer pH 5.8, at 37C
-
0.00009 - 0.0004
D-Mannonolactam amidrazone
2
di-epi-swainsonine
Drosophila melanogaster
-
-
0.00001 - 0.00013
Mannostatin A
0.000005 - 0.00005
swainsonine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.425
-
4-nitrophenyl alpha-D-mannoside as substrate
3.1
-
pH 5.5, 37C
4.2
-
purified recombinant TM1851
5.4
-
purified native enzyme
6.38
purified recombinant GMII expressed in insect cells, substrate 4-nitrophenyl alpha-mannoside
6.75
-
intact 124 kDa Man II
7.03
-
chymotrypsin-cleaved soluble 110 kDa catalytic subunit
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 6.4
-
-
6 - 6.5
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 6
-
pH 3.5: more than 70% of maximal activity, pH 6: about half-maximal activity
3.8 - 9
-
30 min
4.2 - 6
-
pH 4.2: about half-maximal activity, pH 6: about 80% of maximal activity
4.25 - 8
-
-
4.8 - 7
-
about half-maximal activity at pH 4.8 and 7
5.5 - 6.7
-
alpha-mannosidase III activity is negligible at pH 5.5, increases at pH 5.7-5.9, decreases at pH 6.1 and rises to a plateau at pH 6.5-6.7
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
Neosartorya fischeri
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 8.5
-
low activity at neutral pH
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.3
-
for the first isoform of the enzyme, isoelectric focusing
4.6
-
method not mentioned
4.8
-
for the second isoform of the enzyme, isoelectric focusing
5.8
-
pH 3 to 10 gradient, isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
the enzyme is lowly expressed in the molecular layer of the cerebral cortex and hippocampal layers, and highly expressed in other areas of the brain
Manually annotated by BRENDA team
-
clear cells in all epdididymal regions, not in principal cells
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
in the Golgi apparatus
Manually annotated by BRENDA team
-
influenza virus-infected
Manually annotated by BRENDA team
-
freeze-dried mixture of mycelium and its culture medium, commercially available as Morushin
Manually annotated by BRENDA team
-
normal kidney cells
Manually annotated by BRENDA team
-
in the Golgi apparatus
Manually annotated by BRENDA team
-
baculovirus-infected IPLB-SF-21AE cells
Manually annotated by BRENDA team
-
in the Golgi apparatus of Sertoli and Leydig cells
Manually annotated by BRENDA team
additional information
-
tissue distribution of Man II mRNA
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
hepatocytes cultured in dexamethason-supplemented medium
Manually annotated by BRENDA team
-
at least 3 forms of alpha-mannosidase III, the smallest form accumulates in the extracellular fraction
-
Manually annotated by BRENDA team
SpGH38 may be intracellular, absence of any classical signal peptide
Manually annotated by BRENDA team
-
hepatocytes cultured in colchicine-containing medium
Manually annotated by BRENDA team
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
74000
-
SDS-PAGE
81000
-
native-PAGE
97000
-
SDS-PAGE
110000
230000
-
chymotrypsin-cleaved 110 kDa catalytic subunit, gel filtration
240000
-
PAGE under nonreducing conditions
285000
-
intact enzyme, gel filtration
295000
-
tetrameric form, gel filtration
340000
-
gel filtration
468000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
trimer
-
3 * 120000, SDS-PAGE
additional information