Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 25-30% activation, 0.05% | Vigna radiata |
General Stability | Organism |
---|---|
glycerol stabilizes | Vigna radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-Dideoxy-1,4-imino-D-mannitol | much less effective than swainsonine | Vigna radiata | |
alpha-1,6-linked trisaccharide of mannose | 1.5 mM, 40% inhibition | Vigna radiata | |
Cu2+ | 0.2 mM CuCl2, 75% inhibition | Vigna radiata | |
additional information | not inhibited by Co2+, Zn2+; not inhibited by deoxymannojirimycin; not inhibited by EDTA; not inhibited by EGTA, 6-epicastanospermine, 2-episwainsonine, a trisaccharide of mannose residues linked in alpha-1,2-linkages, a tetrasaccharide of mannose with the nonreducing mannose linked in alpha-1,3-linkage to an alpha-1,2-linked trisaccharide of mannose; not inhibited by Mn2+, Mg2+ | Vigna radiata | |
swainsonine | i.e. (1S,2R,8R,8aR)-trihydroxyindolizidine; very strong | Vigna radiata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Vigna radiata | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Vigna radiata | |
additional information | not activated by Ca2+ | Vigna radiata | |
additional information | not activated by Zn2+ | Vigna radiata | |
additional information | not activated by Co2+ | Vigna radiata | |
additional information | not activated by Mg2+, Mn2+ | Vigna radiata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
125000 | - |
x * 125000, SDS-PAGE | Vigna radiata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata | - |
mung bean | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | presumably an N-linked glycoprotein with high-mannose chains | Vigna radiata |
Purification (Comment) | Organism |
---|---|
1200fold | Vigna radiata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Vigna radiata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Vigna radiata |
Storage Stability | Organism |
---|---|
0-4°C, 10 mM HEPES buffer, pH 7.2, 10% glycerol, several days, stable | Vigna radiata |
frozen, 10 mM HEPES buffer, pH 7.1, 0.1% Triton X-100, 0.5 mM PMSF, 0.2 mM dithiothreitol, 10% glycerol, at least 2 months, stable | Vigna radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Man)3GlcNAc + H2O | poor substrate | Vigna radiata | ? | - |
? | |
4-methylumbelliferyl alpha-D-mannoside + H2O | - |
Vigna radiata | 4-methylumbelliferone + alpha-D-mannose | - |
? | |
4-nitrophenyl alpha-D-mannoside + H2O | - |
Vigna radiata | 4-nitrophenol + alpha-D-mannose | - |
? | |
GlcNAc(Man)5GlcNAc + 2 H2O | excellent substrate, removes the alpha-1,3- and alpha-1,6-mannosyl residues with preference for the alpha-1,6-linkage | Vigna radiata | GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose | final product, via GlcNAc(Man)4GlcNAc | ? | |
GlcNAc(Man)5GlcNAc + 2 H2O | excellent substrate, removes the alpha-1,3- and alpha-1,6-mannosyl residues with preference for the alpha-1,6-linkage | Vigna radiata | GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose | product structure: GlcNAcManalpha(1-3)(Manalpha(1-6))ManbetaGlcNAc | ? | |
additional information | not: Glc3(Man)9GlcNAc | Vigna radiata | ? | - |
? | |
additional information | not: (Man)9GlcNAc | Vigna radiata | ? | - |
? | |
additional information | not: (Man)5GlcNAc | Vigna radiata | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 125000, SDS-PAGE | Vigna radiata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Vigna radiata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 7 | about half-maximal activity at pH 4.8 and 7 | Vigna radiata |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
below, high loss of activity within 1 week | Vigna radiata |