Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 50% inhibition | Aspergillus phoenicis | |
additional information | not inhibited by EDTA | Aspergillus phoenicis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.25 | - |
Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc | NaBH4 reduced | Aspergillus phoenicis | |
1.25 | - |
Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc | pH 5, 37°C | Aspergillus phoenicis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | strongly activates, 1 mM, 13fold | Aspergillus phoenicis | |
additional information | not activated by Zn2+ | Aspergillus phoenicis | |
additional information | not activated by Ba2+ | Aspergillus phoenicis | |
additional information | not activated by Mg2+, Mn2+ | Aspergillus phoenicis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus phoenicis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
1000fold | Aspergillus phoenicis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | freeze-dried mixture of mycelium and its culture medium, commercially available as Morushin | Aspergillus phoenicis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
38000 | - |
- |
Aspergillus phoenicis |
Storage Stability | Organism |
---|---|
-20°C or 4°C, 4 months, less than 5% loss of activity | Aspergillus phoenicis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O | NaBH4 reduced, poor substrate | Aspergillus phoenicis | ? | - |
? | |
D-Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O | NaBH4 reduced, good substrate | Aspergillus phoenicis | D-Manbeta(1-4)GlcNAc + alpha-D-mannose | NaBH4 reduced | ? | |
D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | NaBH4 reduced, at lower rate than Manalpha(1-3)Manbeta(1-4)GlcNAc hydrolysis | Aspergillus phoenicis | D-Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | NaBH4 reduced | ? | |
GlcNAcbeta(1-2)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | NaBH4 reduced, hydrolysis at equal rate as Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, galactosyl and sialyl-galactosyl derivatives are substrates, too | Aspergillus phoenicis | ? | - |
? | |
GlcNAcbeta(1-6)(GlcNAcbeta(1-2))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | NaBH4 reduced, rate at 50% of Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc hydrolysis | Aspergillus phoenicis | ? | - |
? | |
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | NaBH4 reduced | Aspergillus phoenicis | Manalpha(1-6)(Manalpha(1-3))D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | NaBH4 reduced | ? | |
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | NaBH4 reduced, cleaves the Manalpha(1-6)Man linkage only after its Manalpha(1-3) residue is removed | Aspergillus phoenicis | Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | NaBH4 reduced, no product: Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc | ? | |
additional information | not: 4-nitrophenyl alpha-mannoside | Aspergillus phoenicis | ? | - |
? | |
additional information | not: D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-4))[GlcNAcbeta(1-4)(GlcNAcbeta(1-2))Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-4)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, all sodium borate reduced | Aspergillus phoenicis | ? | - |
? | |
additional information | substrate specificity, hydrolysis mechanism of branched oligosaccharides | Aspergillus phoenicis | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aspergillus phoenicis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus phoenicis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6 | pH 3.5: more than 70% of maximal activity, pH 6: about half-maximal activity | Aspergillus phoenicis |