Literature summary for 3.2.1.114 extracted from

Nakajima, M.; Fushinobu, S.; Imamura, H.; Shoun, H.; Wakagi, T.
Crystallization and preliminary X-ray analysis of cytosolic alpha-mannosidase from Thermotoga maritima (2006), Acta Crystallogr. Sect. F, 62, 104-105.

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Cloned(Commentary)

Cloned (Comment)	Organism
TM1851, expression in Escherichia coli	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant TM1851, sitting drop vapour diffusion method, optimal conditions: 0.001 ml of protein solution containing 5.3 mg/ml protein in 5 mM sodium phosphate and 150 mM NaCl, pH 6.8, mixed with an equal volume of reservoir solution containing 4% w/v PEG 6000, 50 mM sodium phosphate, pH 6.0, and 0.5 M NaCl, 1 day at 25°C, X-ray diffraction structure determination and preliminary analysis at 2.9 A resolution	Thermotoga maritima

Crystallization (Comment)

Organism

purified recombinant TM1851, sitting drop vapour diffusion method, optimal conditions: 0.001 ml of protein solution containing 5.3 mg/ml protein in 5 mM sodium phosphate and 150 mM NaCl, pH 6.8, mixed with an equal volume of reservoir solution containing 4% w/v PEG 6000, 50 mM sodium phosphate, pH 6.0, and 0.5 M NaCl, 1 day at 25°C, X-ray diffraction structure determination and preliminary analysis at 2.9 A resolution

Thermotoga maritima

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Thermotoga maritima	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cd2+	preferred divalent cation	Thermotoga maritima
Co2+	preferred divalent cation	Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Thermotoga maritima	the exo-type cytosolic class II enzyme cleaves off alpha-1,2-, alpha-1,3-, and alpha-1,6-mannose residues	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant TM1851 from Escherichia coli by ion exchange and hydroxyapatite chromatography, and gel filtration	Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.2	-	purified recombinant TM1851	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the exo-type cytosolic class II enzyme cleaves off alpha-1,2-, alpha-1,3-, and alpha-1,6-mannose residues	Thermotoga maritima	?	-	?

Synonyms

Synonyms	Comment	Organism
class II alpha-mannosidase	-	Thermotoga maritima
More	the enzyme belongs to the glycoside hydrolase family 38	Thermotoga maritima
TM1851	-	Thermotoga maritima