Literature summary for 3.2.1.114 extracted from

Zhong, W.; Kuntz, D.A.; Ember, B.; Singh, H.; Moremen, K.W.; Rose, D.R.; Boons, G.J.
Probing the substrate specificity of Golgi alpha-mannosidase II by use of synthetic oligosaccharides and a catalytic nucleophile mutant (2008), J. Am. Chem. Soc., 130, 8975-8983.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Drosophila melanogaster
mutants D204A and D341N in complex with 2,4-dinitrophenyl-alpha-D-mannopyranoside and in complex with oligosaccharides containing an alpha-(1,6)-or alpha-(1,3)-linked 1-thio-alpha-mannoside	Drosophila melanogaster

Protein Variants

Protein Variants	Comment	Organism
D204A	almost complete loss of activity. Residue D204 is involved in the conformational change of the bound mannoside to a high-energy B2,5 conformation. In the mutant, mannose adopts the low-energy 4C1 conformation	Drosophila melanogaster
D204A	mutant of Drosophila melanogaster, residual mannosidase activity	Drosophila melanogaster
D341N	acid-base mutant, residual mannosidase activity	Drosophila melanogaster
D341N	almost complete loss of activity. Substrate mannose is found in a distorted high energy B2,5 conformation, which is necessary for it to fit in the confined space of the binding pocket	Drosophila melanogaster

Inhibitors

Inhibitors	Comment	Organism	Structure
mannostatin	-	Drosophila melanogaster
swainsonine	-	Drosophila melanogaster

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	-	Drosophila melanogaster	5794	-

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-
Drosophila melanogaster	Q24451	isoform GMII	-

Purification (Commentary)

Purification (Comment)	Organism
-	Drosophila melanogaster

Reaction

Reaction	Comment	Organism	Reaction ID
Man5GlcNAc3-[protein] + 2 H2O = Man3GlcNAc3-[protein] + 2 alpha-D-mannopyranose	the conformational change of the bound mannoside to a high-energy B2,5 conformation is facilitated by steric hindrance from, and the formation of strong hydrogen bonds to, resiude D204	Drosophila melanogaster	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,4-dinitrophenyl alpha-D-mannopyranoside + H2O	-	Drosophila melanogaster	?	-	?
2,4-dinitrophenyl-alpha-D-mannopyranoside + H2O	-	Drosophila melanogaster	2,4-dinitrophenol + D-mannose	-	?
4-methylumbelliferyl alpha-D-mannoside + H2O	-	Drosophila melanogaster	4-methylumbelliferone + alpha-D-mannose	-	?
additional information	about 80-fold preference of isoform GMII for the cleavage of substrates containing a nonreducing beta-(1,2)-linked GlcNAc group	Drosophila melanogaster	?	-	?

Synonyms

Synonyms	Comment	Organism
GmII	-	Drosophila melanogaster
Golgi alpha-mannosidase II	-	Drosophila melanogaster

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Release 2023.1 (January 2023)