Any feedback?
Literature summary for 3.2.1.114 extracted from
- Processing of asparagine-linked oligosaccharides in insect cells: evidence for alpha-mannosidase II (1995), Glycoconj. J., 12, 150-155.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | - |
Vigna radiata |  |
| Triton X-100 | omission results in 40% decrease of activity | Bombyx mori | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 20 mM, 90% inhibition | Bombyx mori | |
| Cu2+ | - |
Spodoptera frugiperda | |
| Cu2+ | - |
Vigna radiata | |
| EDTA | 20 mM, 20% inhibition | Bombyx mori | |
| EDTA | - |
Spodoptera frugiperda | |
| additional information | not inhibited by Ca2+; not inhibited by Mn2+, Mg2+ | Bombyx mori | |
| additional information | - |
Spodoptera frugiperda | |
| additional information | not inhibited by Mn2+, Mg2+ | Vigna radiata | |
| swainsonine | 30 nM, 85% inhibition | Bombyx mori | |
| swainsonine | - |
Spodoptera frugiperda | |
| swainsonine | - |
Vigna radiata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound, predominantly in the microsomal pellet | Bombyx mori | 16020 | - |
| microsome | - |
Vigna radiata | - |
- |
| microsome | predominantly, membrane-bound | Bombyx mori | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metal ion requirement | Bombyx mori | |
| additional information | no metal ion requirement | Vigna radiata | |
| additional information | no metal ion requirement | Spodoptera frugiperda | |
| additional information | not activated by Ca2+ | Bombyx mori | |
| additional information | not activated by Ca2+ | Vigna radiata | |
| additional information | not activated by Ca2+ | Spodoptera frugiperda | |
| additional information | not activated by Mg2+, Mn2+ | Bombyx mori | |
| additional information | not activated by Mg2+, Mn2+ | Vigna radiata | |
| additional information | not activated by Mg2+, Mn2+ | Spodoptera frugiperda |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
- |
- |
| Bombyx mori Bm-N | - |
- |
- |
| Mamestra brassicae | - |
- |
- |
| Mamestra brassicae Mb-0503 | - |
- |
- |
| Spodoptera frugiperda | - |
Sf-21 | - |
| Spodoptera frugiperda Sf-21 | - |
Sf-21 | - |
| Vigna radiata | - |
mung bean | - |
| Xenopus laevis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | frog | Xenopus laevis | - |
| seedling | - |
Vigna radiata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-mannoside + H2O | - |
Bombyx mori | 4-nitrophenol + alpha-D-mannose | - |
? | |
| 4-nitrophenyl alpha-D-mannoside + H2O | - |
Vigna radiata | 4-nitrophenol + alpha-D-mannose | - |
? | |
| 4-nitrophenyl alpha-D-mannoside + H2O | - |
Spodoptera frugiperda | 4-nitrophenol + alpha-D-mannose | - |
? | |
| 4-nitrophenyl alpha-D-mannoside + H2O | - |
Spodoptera frugiperda Sf-21 | 4-nitrophenol + alpha-D-mannose | - |
? | |
| 4-nitrophenyl alpha-D-mannoside + H2O | - |
Bombyx mori Bm-N | 4-nitrophenol + alpha-D-mannose | - |
? | |
| additional information | - |
Vigna radiata | ? | - |
? | |
| additional information | not: (Man)5(GlcNAc)2 | Bombyx mori | ? | - |
? | |
| additional information | not: (Man)5(GlcNAc)2 | Spodoptera frugiperda | ? | - |
? | |
| additional information | not: (Man)5(GlcNAc)2 | Spodoptera frugiperda Sf-21 | ? | - |
? | |
| additional information | not: (Man)5(GlcNAc)2 | Bombyx mori Bm-N | ? | - |
? | |
| pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Spodoptera frugiperda | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
| pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Mamestra brassicae | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
| pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | via the intermediate product GlcNAc(Man)4(GlcNAc)2, the alpha-1,6-linked mannosyl residue is removed first, then the 1,3-linked | Bombyx mori | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
| pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | via the intermediate product GlcNAc(Man)4(GlcNAc)2, the alpha-1,6-linked mannosyl residue is removed first, then the 1,3-linked | Xenopus laevis | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
| pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | via the intermediate product GlcNAc(Man)4(GlcNAc)2, the alpha-1,6-linked mannosyl residue is removed first, then the 1,3-linked | Vigna radiata | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
| pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | dependent on the prior action of N-acetylglucosaminyl transferase I on (Man)5(GlcNAc)2 | Bombyx mori | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
| pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Spodoptera frugiperda Sf-21 | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
| pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | via the intermediate product GlcNAc(Man)4(GlcNAc)2, the alpha-1,6-linked mannosyl residue is removed first, then the 1,3-linked | Bombyx mori Bm-N | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
| pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | dependent on the prior action of N-acetylglucosaminyl transferase I on (Man)5(GlcNAc)2 | Bombyx mori Bm-N | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
| pyridylaminated D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Mamestra brassicae Mb-0503 | pyridylaminated D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bombyx mori |
| 37 | - |
assay at | Vigna radiata |
| 37 | - |
assay at | Spodoptera frugiperda |
| 37 | - |
assay at | Mamestra brassicae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Bombyx mori |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
