Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 91-93% inhibition; strong | Rattus norvegicus | |
Fe2+ | 1 mM, 70-76% inhibition; strong | Rattus norvegicus | |
Fe3+ | less potent inhibitor than Fe2+ and Cu2+, 1 mM, 24-30% inhibition | Rattus norvegicus | |
additional information | not inhibited by Co2+, Zn2+; not inhibited by EDTA; not inhibited by Tris-Cl, iodoacetamide, alpha-methylmannoside; not inhibited by Tris-maleate | Rattus norvegicus | |
p-chloromercuriphenylsulfonate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | - |
Rattus norvegicus | 139 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation | Rattus norvegicus | |
Zn2+ | activation | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | biosynthesis of complex-type N-linked oligosaccharides of glycoproteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar rats | - |
Purification (Comment) | Organism |
---|---|
20fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.425 | - |
4-nitrophenyl alpha-D-mannoside as substrate | Rattus norvegicus |
Storage Stability | Organism |
---|---|
2-4°C, 10 mM potassium phosphate buffer, pH 7.2, 1% Triton X-100, 4 weeks, 20% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Man)6GlcNAc + H2O | rate at 2% of GlcNAc(Man)5GlcNAc hydrolysis | Rattus norvegicus | ? | - |
? | |
(Man)7GlcNAc + H2O | rate at 3% of GlcNAc(Man)5GlcNAc hydrolysis | Rattus norvegicus | ? | - |
? | |
(Man)8GlcNAc + H2O | rate at 5% of GlcNAc(Man)5GlcNAc hydrolysis | Rattus norvegicus | ? | - |
? | |
(Man)9GlcNAc + H2O | rate at 6.4% of GlcNAc(Man)5GlcNAc hydrolysis | Rattus norvegicus | ? | - |
? | |
4-nitrophenyl alpha-D-mannoside + H2O | rate at 23.5% of GlcNAc(Man)5GlcNAc hydrolysis | Rattus norvegicus | 4-nitrophenol + alpha-D-mannose | - |
? | |
D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Rattus norvegicus | D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose | - |
? | |
GlcNAc(Man)5GlcNAc + 2 H2O | preferred substrate | Rattus norvegicus | GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose | - |
? | |
additional information | not: (Man)5GlcNAc | Rattus norvegicus | ? | - |
? | |
additional information | biosynthesis of complex-type N-linked oligosaccharides of glycoproteins | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
24 h, in 100 mM sodium acetate buffer, pH 5.8, 30-50% loss of activity | Rattus norvegicus |
50 | - |
3 h, about 30% loss of activity, 4-nitrophenyl alpha-D-mannoside or (Man)9GlcNAc as substrate | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
4-nitrophenyl alpha-D-mannoside hydrolysis | Rattus norvegicus |
5.8 | - |
(Man)8GlcNAc as substrate | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
1 h, at 37°C, inactivation, 1 mM ZnCl2 partially stabilizes | Rattus norvegicus |
6 | - |
1 h, at 37°C, 30% loss of activity | Rattus norvegicus |
8 | - |
1 h, at 37°C, stable | Rattus norvegicus |