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Literature summary for 3.2.1.114 extracted from
- Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans (1991), J. Cell Biol., 115, 1521-1534.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | reduced expression of Man II in the autosomal genetic HEMPAS disease, i.e. hereditary erythroblastic multinuclearity associated with positive acidified serum | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full-length cDNA is cloned and sequenced, 8-12fold overexpression in COS cells | Mus musculus |
| isolation of partial cDNA clones, gene is located on chromosome 5 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| swainsonine | - |
Homo sapiens |  |
| swainsonine | - |
Mus musculus | |
| swainsonine | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi membrane | - |
Homo sapiens | 139 | - |
| Golgi membrane | - |
Rattus norvegicus | 139 | - |
| Golgi membrane | type II transmembrane protein with short cytoplasmic tail, single transmembrane domain and large C-terminal catalytic domain | Mus musculus | 139 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 131000 | - |
x * 131000, predicted from the amino acid sequence | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | Man II catalyzes the final hydrolytic step in the N-glycan maturation pathway acting as the committed step in the conversion of high mannose to complex type structures | ? | - |
? | |
| additional information | Rattus norvegicus | Man II catalyzes the final hydrolytic step in the N-glycan maturation pathway acting as the committed step in the conversion of high mannose to complex type structures | ? | - |
? | |
| additional information | Mus musculus | Man II catalyzes the final hydrolytic step in the N-glycan maturation pathway acting as the committed step in the conversion of high mannose to complex type structures | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | P27046 | - |
- |
| Rattus norvegicus | - |
male sprague-dawley rats | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
| glycoprotein | - |
Rattus norvegicus |
| glycoprotein | - |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Hep-G2 cell | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| liver | rat | Rattus norvegicus | - |
| additional information | tissue distribution of Man II mRNA | Rattus norvegicus | - |
| NIH-3T3 cell | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
| additional information | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl alpha-D-mannoside + H2O | - |
Homo sapiens | 4-methylumbelliferone + alpha-D-mannose | - |
? | |
| 4-methylumbelliferyl alpha-D-mannoside + H2O | - |
Rattus norvegicus | 4-methylumbelliferone + alpha-D-mannose | - |
? | |
| 4-methylumbelliferyl alpha-D-mannoside + H2O | - |
Mus musculus | 4-methylumbelliferone + alpha-D-mannose | - |
? | |
| additional information | domain structure | Rattus norvegicus | ? | - |
? | |
| additional information | domain structure | Mus musculus | ? | - |
? | |
| additional information | Man II catalyzes the final hydrolytic step in the N-glycan maturation pathway acting as the committed step in the conversion of high mannose to complex type structures | Homo sapiens | ? | - |
? | |
| additional information | Man II catalyzes the final hydrolytic step in the N-glycan maturation pathway acting as the committed step in the conversion of high mannose to complex type structures | Rattus norvegicus | ? | - |
? | |
| additional information | Man II catalyzes the final hydrolytic step in the N-glycan maturation pathway acting as the committed step in the conversion of high mannose to complex type structures | Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Homo sapiens |
| ? | - |
Rattus norvegicus |
| ? | x * 131000, predicted from the amino acid sequence | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Homo sapiens |
| 5.5 | - |
- |
Rattus norvegicus |
| 5.5 | - |
endogenous enzyme in 3T3 cells and recombinant enzyme expressed in COS cells | Mus musculus |
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