Crystallization (Comment) | Organism |
---|---|
crystal structure of enzyme with bound inhibitors kifunensine, swainsonine and 1-deoxymannojirimycin | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxymannojirimycin | more inhibitory than kifunensine, mode of binding | Drosophila melanogaster | |
kifunensine | weak, mode of binding | Drosophila melanogaster | |
swainsonine | mode of binding; very strong | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Drosophila melanogaster | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | active site coordination to Zn2+ | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O | Drosophila melanogaster | N-glycosylation pathway | Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
PDB: 1PS3 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Man5GlcNAc3-[protein] + 2 H2O = Man3GlcNAc3-[protein] + 2 alpha-D-mannopyranose | catalytic mechanism | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-mannoside + H2O | - |
Drosophila melanogaster | 4-nitrophenol + alpha-D-mannose | - |
? | |
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O | N-glycosylation pathway | Drosophila melanogaster | Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | member of glycosyl hydrolase family 38 | Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
- |
Drosophila melanogaster |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000105 | - |
swainsonine | pH 5.75, 37°C | Drosophila melanogaster | |
5.2 | - |
kifunensine | pH 5.75, 37°C | Drosophila melanogaster |