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Literature summary for 3.2.1.114 extracted from

  • Zhu, Y.; Suits, M.D.; Thompson, A.J.; Chavan, S.; Dinev, Z.; Dumon, C.; Smith, N.; Moremen, K.W.; Xiang, Y.; Siriwardena, A.; Williams, S.J.; Gilbert, H.J.; Davies, G.J.
    Mechanistic insights into a Ca2+-dependent family of alpha-mannosidases in a human gut symbiont (2010), Nat. Chem. Biol., 6, 125-132.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.114

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
 4-nitrophenyl alpha-D-mannopyranoside pH 7.0, 37°C, Bt3991 Homo sapiens
9
-
 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose pH 7.0, 37°C, Bt3991 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O Homo sapiens
-
 alpha-D-mannopyranose
-
 ?
Man5GlcNAc2 + H2O Homo sapiens
-
 Man3GlcNAc2 + 2 alpha-D-mannopyranose
-
 ?
additional information Homo sapiens Bt1769, Bt3858 and Bt3991 are alpha-1,3-mannosidases that display particularly high activity against alpha-1,3-mannobiose ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Homo sapiens
-
gut
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O
-
 Homo sapiens alpha-D-mannopyranose
-
 ?
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O Bt3991 displays particularly high activity against alpha-1,3-mannobiose Homo sapiens 2 alpha-D-mannopyranose
-
 ?
4-nitrophenyl alpha-D-mannopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + alpha-D-mannopyranose
-
 ?
Man5GlcNAc2 + H2O
-
 Homo sapiens Man3GlcNAc2 + 2 alpha-D-mannopyranose
-
 ?
Man5GlcNAc2 + H2O the alpha-1,3-mannosidase Bt3991, while unable to hydrolyze Man9GlcNAc2, converts Man5GlcNAc2 into Man3GlcNAc2 hydrolyzing the distal alpha-1,6-mannosidic linkage in high-mannose N-glycans. The alpha-1,3-mannosidic linkages in N-glycans are available to the enzyme once the terminal alpha-1,2-linked mannosyl residues are removed Homo sapiens Man3GlcNAc2 + 2 alpha-D-mannopyranose
-
 ?
additional information Bt1769, Bt3858 and Bt3991 are alpha-1,3-mannosidases that display particularly high activity against alpha-1,3-mannobiose Homo sapiens ?
-
 ?
additional information alpha-1,3-mannosidases, Bt3858 and Bt1769, convert Man4GlcNAc2 to Man3GlcNAc2, but do not attack Man5GlcNAc2, indicat­ing that these enzymes cannot tolerate alpha-1,6-Man side chains Homo sapiens ?
-
 ?
ovalbumin + H2O
-
 Homo sapiens ?
-
 ?
yeast mannan + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-1,3-mannosidase
-
 Homo sapiens
Bt3991
-
 Homo sapiens
More Bt3991 belongs to the glycosyl hydrolase family 92, GH92 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0025
-
 4-nitrophenyl alpha-D-mannopyranoside pH 7.0, 37°C, Bt3991 Homo sapiens
4.67
-
 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose pH 7.0, 37°C, Bt3991 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0009
-
 4-nitrophenyl alpha-D-mannopyranoside pH 7.0, 37°C, Bt3991 Homo sapiens
0.52
-
 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose pH 7.0, 37°C, Bt3991 Homo sapiens