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Literature summary for 3.2.1.114 extracted from

  • Oh-Eda, M.; Nakagawa, H.; Akama, T.O.; Lowitz, K.; Misago, M.; Moremen, K.W.; Fukuda, M.N.
    Overexpression of the Golgi-localized enzyme alpha-mannosidase IIx in Chinese hamster ovary cells results in the conversion of hexamannosyl-N-acetylchitobiose to tetramannosyl-N-acetylchitobiose in the N-glycan-processing pathway (2001), Eur. J. Biochem., 268, 1280-1288.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
alpha-mannosidase II and IIx, expression in COS cells as protein A fusion, overexpression of alpha-mannosidase IIx in CHO-K1 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
swainsonine inhibits both alpha-mannosidase II and IIx, the latter is less sensitive Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane type II membrane protein, alpha-mannosidase II and IIx Homo sapiens 139
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O Homo sapiens alpha-mannosidase IIx, M6Gn2 is the primary target in vivo, integral part of N-glycan biosynthesis Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O Homo sapiens alpha-mannosidase II, biosynthesis of N-glycans Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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alpha-mannosidase II and IIx
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Purification (Commentary)

Purification (Comment) Organism
recombinant protein A fusion of alpha-mannosidase II and IIx, expressed in COS cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell alpha-mannosidase IIx Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-D-mannoside + H2O catalytic domain of alpha-mannosidase IIx Homo sapiens 4-methylumbelliferone + alpha-D-mannose
-
?
4-nitrophenyl alpha-D-mannoside + H2O alpha-mannosidase IIx Homo sapiens 4-nitrophenol + alpha-D-mannose
-
?
D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O alpha-mannosidase II Homo sapiens D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O recombinant alpha-mannosidase IIx, expressed in CHO cells, hydrolyzes 2 peripheral Manapha(1-6) and Manalpha(1-3) residues Homo sapiens Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O alpha-mannosidase IIx, M6Gn2 is the primary target in vivo, integral part of N-glycan biosynthesis Homo sapiens Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O alpha-mannosidase II Homo sapiens Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 H2O alpha-mannosidase II, biosynthesis of N-glycans Homo sapiens Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
-
?

Synonyms

Synonyms Comment Organism
alpha-mannosidase IIx homolog to alpha-mannosidase II with different substrate specificity Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
recombinant alpha-mannosidase IIx Homo sapiens