Information on EC 3.1.3.25 - inositol-phosphate phosphatase

New: Word Map on EC 3.1.3.25
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
3.1.3.25
-
RECOMMENDED NAME
GeneOntology No.
inositol-phosphate phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
myo-inositol phosphate + H2O = myo-inositol + phosphate
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
-
D-myo-inositol (1,4,5)-trisphosphate degradation
-
-
Inositol phosphate metabolism
-
-
Metabolic pathways
-
-
myo-inositol biosynthesis
phytate degradation I
-
-
Streptomycin biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
myo-inositol-phosphate phosphohydrolase
Acts on five of the six isomers of myo-inositol phosphate, all except myo-inositol 2-phosphate, but does not act on myo-inositol bearing more than one phosphate group. It also acts on adenosine 2'-phosphate (but not the 3'- or 5'- phosphates), sn-glycerol 3-phosphate and glycerol 2-phosphate. Two isoforms are known [4].
CAS REGISTRY NUMBER
COMMENTARY hide
37184-63-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
Anabaena sp. PCC7120
-
-
-
Manually annotated by BRENDA team
strain AF2372
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
cv. Harrington, wild-type and mutant
SwissProt
Manually annotated by BRENDA team
strain MJ0109
-
-
Manually annotated by BRENDA team
strain MJ109
Uniprot
Manually annotated by BRENDA team
strain MC2 155
-
-
Manually annotated by BRENDA team
strain MC2 155
-
-
Manually annotated by BRENDA team
strain H37Rv, gene Rv2131c
-
-
Manually annotated by BRENDA team
Mycobacterium tuberculosis H37Rv, ATCC 25618
genes impA or Rv1604, and impC or Rv3137
-
-
Manually annotated by BRENDA team
bv. trifolii
-
-
Manually annotated by BRENDA team
strain PCC 6803
-
-
Manually annotated by BRENDA team
strain TM1415
-
-
Manually annotated by BRENDA team
additional information
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
existence of three distinct evolutionary groups of enzyme: vertebrate IMPA1 proteins, vertebrate IMPA2 proteins and invertebrate IMPA proteins
malfunction
metabolism
myo-inositol monophosphatase catalyzes the dephosphorylation of myo-inositol 3-phosphate in the last step of myo-inositol biosynthesis. Expression of IMP genes is correlated with expression of the gene for myo-inositol polyphosphate 1-phosphatase, which is involved in the myo-inositol salvage pathway, suggesting a possible salvage pathway role in seed development. Moreover, the partial rescue of the impl2 phenotype by histidine application implies that IMPL2 is also involved in histidine biosynthesis during embryo development; myo-inositol monophosphatase catalyzes the dephosphorylation of myo-inositol 3-phosphate in the last step of myo-inositol biosynthesis. Expression of IMP genes is correlated with expression of the gene for myo-inositol polyphosphate 1-phosphatase, which is involved in the myo-inositol salvage pathway, suggesting a possible salvage pathway role in seed development. Moreover, the partial rescue of the impl2 phenotype by histidine application implies that IMPL2 is also involved in histidine biosynthesis during embryo development; myo-inositol monophosphatase catalyzes the dephosphorylation of myo-inositol 3-phosphate in the last step of myo-inositol biosynthesis. Expression of IMP genes is correlated with expression of the gene for myo-inositol polyphosphate 1-phosphatase, which is involved in the myo-inositol salvage pathway, suggesting a possible salvage pathway role in seed development. Moreover, the partial rescue of the impl2 phenotype by histidine application implies that IMPL2 is also involved in histidine biosynthesis during embryo development
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(-)-(1R,2R,4R,6R)-2,4,6-trihydroxycyclohexyl cyclohexylammonium 1-hydroxyphosphonate + H2O
?
show the reaction diagram
-
-
-
-
?
2'-AMP + H2O
adenosine + phosphate
show the reaction diagram
2'-CMP + H2O
cytidine + phosphate
show the reaction diagram
2'-GMP + H2O
guanosine + phosphate
show the reaction diagram
2'-phosphoadenosine 5'-phosphate + H2O
?
show the reaction diagram
2'-UMP + H2O
uridine + phosphate
show the reaction diagram
2,3-diphosphoglycerate + H2O
?
show the reaction diagram
-
-
-
-
?
2-phosphoglycerate + H2O
?
show the reaction diagram
2-phosphoglycerol + H2O
glycerol + phosphate
show the reaction diagram
2-phosphoglycolate + H2O
?
show the reaction diagram
-
-
-
-
?
3'-AMP + H2O
adenosine + phosphate
show the reaction diagram
3'-CMP + H2O
cytidine + phosphate
show the reaction diagram
-
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
-
?
3'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
-
?
3,5-dideoxy-D-myo-inositol 1-phosphate + H2O
3,5-dideoxy-D-myo-inositol + phosphate
show the reaction diagram
3,5-dideoxy-L-myo-inositol 1-phosphate + H2O
3,5-dideoxy-L-myo-inositol + phosphate
show the reaction diagram
3-deoxy-D-myo-inositol 1-phosphate + H2O
3-deoxy-D-myo-inositol + phosphate
show the reaction diagram
3-deoxy-L-myo-inositol 1-phosphate + H2O
3-deoxy-L-myo-inositol + phosphate
show the reaction diagram
3-phosphoglycerate + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
5'-CMP + H2O
cytidine + phosphate
show the reaction diagram
-
-
-
-
?
5'-dAMP + H2O
adenosine + phosphate
show the reaction diagram
-
-
-
-
?
5'-GMP + H2O
guanosine + phosphate
show the reaction diagram
-
-
-
-
?
5'-UMP + H2O
uridine + phosphate
show the reaction diagram
-
-
-
-
?
6-phosphogluconate + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-D-galactose 1-phosphate + H2O
alpha-D-galactose + phosphate
show the reaction diagram
-
-
-
-
?
alpha-D-glucose 1-phosphate
alpha-D-glucose + phosphate
show the reaction diagram
IMPA1 shows intermediate activity towards alpha-D-glucose 1-phosphate, whereas IMPA2 shows significantly lower affinity
-
-
?
alpha-D-glucose 1-phosphate + H2O
alpha-D-glucose + phosphate
show the reaction diagram
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
-
?
D-alpha-galactose 1-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
D-alpha-glucose 1-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
D-beta glucose 1-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
D-erythrose 4-phosphate + H2O
D-erythrose + phosphate
show the reaction diagram
-
-
-
-
?
D-fructose 1,6-bisphosphate + H2O
D-fructose 6-phosphate + phosphate
show the reaction diagram
D-fructose 1-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
D-fructose 2,6-bisphosphate + H2O
D-fructose 6-phosphate + phosphate
show the reaction diagram
D-fructose 6-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
-
-
-
-
?
D-glucitol-6-phosphate + H2O
D-glucitol + phosphate
show the reaction diagram
-
hydrolyzed very slowly
-
-
?
D-glucose 1-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
D-glucose 6-phosphate + H2O
?
show the reaction diagram
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
D-mannitol-6-phosphate + H2O
D-mannitol + phosphate
show the reaction diagram
-
hydrolyzed very slowly
-
-
?
D-mannose 6-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
D-myo-inositol 1,2,3,4,5,6-hexakisphosphate + H2O
D-myo-inositol 1,2,3,4,5-pentakisphosphate + phosphate
show the reaction diagram
-
-
-
-
?
D-myo-inositol 1,4-bisphosphate + H2O
?
show the reaction diagram
D-myo-inositol 1-phosphate + H2O
D-myo-inositol + phosphate
show the reaction diagram
D-myo-inositol 2-phosphate + H2O
D-myo-inositol + phosphate
show the reaction diagram
D-myo-inositol 3-phosphate + H2O
D-myo-inositol + phosphate
show the reaction diagram
D-ribose 5-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
show the reaction diagram
-
-
-
-
?
D-xylose 5-phosphate + H2O
D-xylose + phosphate
show the reaction diagram
-
-
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
-
-
-
?
DL-myo-inositol 1-phosphate + H2O
DL-myo-inositol + phosphate
show the reaction diagram
fructose 1-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
fructose 6-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
glucitol 6-phosphate + H2O
glucitol + phosphate
show the reaction diagram
-
-
-
?
glucose 1-phosphate + H2O
?
show the reaction diagram
glycerol 2-phosphate + H2O
glycerol + phosphate
show the reaction diagram
inosine 5'-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
inositol 1-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
inositol 4-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
-
the inositol ring docks to the enzyme by forming four hydrogen bonds: the 2-OH group with Asp104, the 3-OH with Asp231, the 5-OH group with Thr106, and the 6-OH with the carbonyl group of main chain of Gly205
-
-
?
L-chiro-inositol-3-phosphate + H2O
?
show the reaction diagram
L-glycerol 1-phosphate + H2O
glycerol + phosphate
show the reaction diagram
L-myo-inositol 1-phosphate + H2O
L-myo-inositol + phosphate
show the reaction diagram
mannitol 1-phosphate + H2O
mannitol + phosphate
show the reaction diagram
-
-
-
?
myo-inositol 1,3-thiophosphate + H2O
?
show the reaction diagram
-
reaction 3times slower than reaction with inositol 1-phosphate
-
?
myo-inositol 1,4-bisphosphate + H2O
?
show the reaction diagram
-
-
-
?
myo-inositol 1-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
myo-inositol 2-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
myo-inositol 3-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
myo-inositol 4-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
myo-inositol 5-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
myo-inositol 6-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
myo-inositol phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
phosphatidylinositol 4,5-bisphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
propan-1-ol 2-phosphate + H2O
1-propanol + phosphate
show the reaction diagram
-
-
-
-
?
purine nucleotides + H2O
?
show the reaction diagram
-
-
-
-
?
pyrimidine ribonucleotides + H2O
?
show the reaction diagram
-
-
-
-
?
scyllo-inositol 1-phosphate + H2O
scyllo-inositol + phosphate
show the reaction diagram
IMPA1 shows strong activity towards scyllo-inositol 1-phosphate, whereas IMPA2 shows almost no affinity
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-fructose 1,6-bisphosphate + H2O
D-fructose 6-phosphate + phosphate
show the reaction diagram
D-myo-inositol 1-phosphate + H2O
D-myo-inositol + phosphate
show the reaction diagram
O30298
-
-
-
?
inositol 1-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
L-myo-inositol 1-phosphate + H2O
L-myo-inositol + phosphate
show the reaction diagram
O30298
-
-
-
?
myo-inositol 1-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
myo-inositol 3-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
myo-inositol 4-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol 5-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol 6-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
-
in its absence activation by Mg2+ occurres at 2fold lower concentrations
Ni2+
-
partial activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(-)-(1R,2R,4R,6R)-2,4,6-trihydroxycyclohexyl 1-methylphosphonate
-
IC50 is 260 mM
(-)-(1R,2R,4R,6R)-6-propyloxy-2,4-dihydroxycyclohexyl 1-ethylphosphonate
-
competitive inhibition
(-)-(1R,2R,4R,6R)-6-propyloxy-2,4-dihydroxycyclohexyl 1-methylphosphonate
-
competitive inhibition
(-)-(1S,2R,4R,6R)-6-propyloxycyclohexane-1,2,4-triol
-
IC50 is about 150 mM
(-)-(1S,2R,4S,6R)-6-(2-aminoethyloxy)cyclohexane-1,2,4-triol
-
IC50 is 5 mM
(-)-(1S,2R,4S,6R)-6-(2-phenylethyl)amino-2,4-dihydroxycyclohexyl 1-methylphosphonate
-
IC50 is above 54 mM
(-)-(1S,2R,4S,6R)-6-(2-phenylethyl)aminocyclohexane-1,2,4-triol
-
IC50 is 6 mM
(-)-(1S,2R,4S,6R)-6-(3-aminopropyloxy)cyclohexane-1,2,4-triol
-
IC50 is 10 mM
(-)-(1S,2R,4S,6R)-6-(4-phenylbutyl)aminocyclohexane-1,2,4-triol
-
non-competitive inhibition
(-)-(1S,2R,4S,6R)-6-aminocyclohexane-1,2,4-triol
-
IC50 is above 50 mM
(-)-(1S,2R,4S,6R)-6-butylaminocyclohexane-1,2,4-triol
-
non-competitive inhibition in absence of phosphate
(-)-(1S,2R,4S,6R)-6-ethylaminocyclohexane-1,2,4-triol
-
IC50 is above 50 mM
(-)-(1S,2R,4S,6R)-6-hexylamino-2,4-dihydroxycyclohexyl 1-methylphosphonate
-
IC50 is 1.3 mM
(-)-(1S,2R,4S,6R)-6-hexylaminocyclohexane-1,2,4-triol
-
uncompetitive inhibition
(-)-(1S,2R,4S,6R)-6-hexyloxycyclohexane-1,2,4-triol
-
IC50 is about 10 mM
(-)-(1S,2R,4S,6R)-6-methylamino-2,4-dihydroxycyclohexyl 1-methylphosphonate
-
IC50 is above 8 mM
(-)-(1S,2R,4S,6R)-6-octylaminocyclohexane-1,2,4-triol
-
IC50 is 4 mM
(-)-(1S,2R,4S,6R)-6-[4-(2-hydroxyphenyloxy)butyloxy]cyclohexane-1,2,4-triol
-
competitive inhibition
(-)-(1S,2R,4S,6R)-6-[N-(4-phenylbutyl)-N-(2-aminoethyl)-amino]cyclohexane-1,2,4-triol [hydrochloride salt]
-
IC50 is 9 mM
(1R,2R,4R,6R)-2,4-dihydroxy-6-[4-(2-hydroxyphenyl)butoxy]cyclohexyl phosphate
-
-
(1S,2R,4S,6R)-6-(butylamino)cyclohexane-1,2,4-triol
-
-
(NH4)2SO4
-
30% inhibition at 10 mM
(phenylmethanediyl)bis(phosphonic acid)
-
-
1-(4-hydroxyphenyloxy)ethane-1,1-bisphosphonic acid
2,3,7-trihydroxycyclohepta-2,4,6-trien-1-one
-
-
2-deoxyglucose-6-phosphate
-
-
2-mercaptoethanol
-
-
3-phosphoglycerate
-
weak competetive inhibitor
6-phosphogluconate
-
slight
acetonitrile
-
-
ATP
-
-
Carbachol
-
in combination with Li
Cr2+
-
50% inhibition at 3 mM
Dimethylsulfoxide
-
5% inhibition
Disulfiram
-
-
dithiothreitol
-
-
DL-myo-inositol 1-phosphate
-
Fe2+
-
-
Heavy metal ions
-
-
-
iodoacetate
-
30% inhibition at 10 mM
L-690330
-
bisphosphonate enzyme inhibitor
L-690488
-
a tetrapivaloyloxymethyl ester prodrug of L-690330
molybdate
-
-
monoclonal antibody [DV1]
-
one of five antibodies was found to inhibit the enzyme activity
-
myo-inositol
myo-inositol 1,3,4,5,6 pentakis(dihydrogen phosphate)
-
-
myo-Inositol tetrakis(dihydrogen phosphate)
-
-
N-ethylmaleimide
-
substrate and Mg2+ protect
Na2MoO4
-
30% inhibition at 10 mM
O2
-
activity of the native enzyme but not the C150S mutant can be completely abolished with oxygen, reversible with thiol-containing compounds
p-hydroxymercuribenzoate
Phenylmercuric nitrate
-
-
phosphate
phosphosulfate
-
-
PO43-
Q6G709
competitive inhibition
pyridoxal-5'-phosphate
-
inactivates the enzyme by covalent binding to the lysine residue of a specific binding site LQVSQQEDITK at or near the substrate binding site, the substrate D-myo-inositol 1-phosphate protects against the inactivation
ribose-5-phosphate
-
-
sulfhydryl reagents
thioredoxin
-
inhibits native enzyme but nut C150S mutant
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
calbindin
-
apo and Ca2+-bound form, strong activation, Kd: approximately 0.001 mM, at pH 6 very low activity in the absence of calbindin, almost no activity lost in the presence of calbindin compared to activity at pH 7
-
Mn2+
activating
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.025
(-)-(1R,2R,4R,6R)-2,4,6-trihydroxycyclohexyl-1-hydroxyphosphonate cyclohexylammonium salt
-
pH 8.0, 25C
0.044
1-L-chiro-inositol-3-phosphate
-
-
0.083 - 1.58
2'-AMP
0.09
2'-GMP
-
-
0.372
3'-AMP
-
-
0.051 - 0.16
3,5-dideoxy-D-myo-inositol 1-phosphate
0.1 - 4.9
3,5-dideoxy-L-myo-inositol 1-phosphate
0.047 - 0.23
3-deoxy-D-myo-inositol 1-phosphate
0.2 - 2.3
3-deoxy-L-myo-inositol 1-phosphate
3.8 - 5
4-nitrophenyl phosphate
0.038
D-fructose 1,6-bisphosphate
85C
2
D-glucitol-6-phosphate
-
-
0.078 - 0.13
D-inositol 1-phosphate
0.12
D-inositol-1-phosphate
-
-
0.148
D-inositol-1-phosphte
-
-
0.82
D-mannitol-6-phosphate
-
-
0.06 - 1.02
D-myo-Inositol 1-phosphate
0.061 - 1.7
D-myo-inositol 3-phosphate
0.0034 - 1.67
DL-myo-inositol 1-phosphate
1.71
glucose-6-phosphate
-
-
0.061 - 4.17
Glycerol 2-phosphate
0.21
inositol 1,3-thiophosphate
-
pH 9.0, 25C
0.286 - 0.733
inositol 2-phosphate
0.11
inositol 3-phosphate
-
pH 9.0, 25C
0.35
inositol-2-phosphate
-
-
0.382 - 0.87
L-glycerol 1-phosphate
0.079
L-inositol 1-phosphate
-
pH 8.0, 37C
0.061 - 13.2
L-myo-Inositol 1-phosphate
0.12
L-myo-inositol-1-phosphate
-
-
3.2
Mg2+
-
pH 7.5, 37C
0.064 - 66
myo-inositol 1-phosphate
15
Propan-1-ol 2-phosphate
-
-
0.235
Sodium diphosphate
-
at 37C, in 25 mM phosphate buffer with 2 mM dithiothreitol (pH 7.0) and 2 mM MgCl2
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.49 - 4
3,5-dideoxy-D-myo-inositol 1-phosphate
4.4 - 5.8
3,5-dideoxy-L-myo-inositol 1-phosphate
3.9 - 4.4
3-deoxy-D-myo-inositol 1-phosphate
3.2 - 5.6
3-deoxy-L-myo-inositol 1-phosphate
2.7 - 268
D-fructose 1,6-bisphosphate
0.165 - 104
D-myo-Inositol 1-phosphate
1.4 - 3.6
D-myo-inositol 3-phosphate
1.29
DL-myo-inositol 1-phosphate
Hordeum vulgare
B3DFH0
recombinant protein
0.19
inositol 1,3-thiophosphate
Bos taurus
-
pH 9.0, 25C
0.48
inositol 3-phosphate
Bos taurus
-
pH 9.0, 25C
1.4 - 16.6
L-myo-Inositol 1-phosphate
1.5 - 207
myo-inositol 1-phosphate
additional information
3,5-dideoxy-L-myo-inositol 1-phosphate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.16
(-)-(1R,2R,4R,6R)-6-propyloxy-2,4-dihydroxycyclohexyl 1-ethylphosphonate
-
pH 8.0, 25C
0.04
(-)-(1R,2R,4R,6R)-6-propyloxy-2,4-dihydroxycyclohexyl 1-methylphosphonate
-
pH 8.0, 25C
9
(-)-(1S,2R,4S,6R)-6-(4-phenylbutyl)aminocyclohexane-1,2,4-triol
-
pH 8.0, 25C
0.3
(-)-(1S,2R,4S,6R)-6-butylaminocyclohexane-1,2,4-triol
4
(-)-(1S,2R,4S,6R)-6-[4-(2-hydroxyphenyloxy)butyloxy]cyclohexane-1,2,4-triol
-
pH 8.0, 25C
0.3
(1S,2R,4S,6R)-6-(butylamino)cyclohexane-1,2,4-triol
-
-
3.84
DL-myo-inositol 1-phosphate
recombinant protein
4.35 - 81
Li+
88
Mg2+
Q6G709
pH and temperature not specified in the publication
39
myo-inositol
-
pH 7.5, 37C
2.7
phosphate
-
pH 7.5, 37C
0.5
phosphosulfate
-
pH 6.5 and pH 9.0
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
260
(-)-(1R,2R,4R,6R)-2,4,6-trihydroxycyclohexyl 1-methylphosphonate
Bos taurus
-
IC50 is 260 mM
150
(-)-(1S,2R,4R,6R)-6-propyloxycyclohexane-1,2,4-triol
Bos taurus
-
IC50 is about 150 mM
5
(-)-(1S,2R,4S,6R)-6-(2-aminoethyloxy)cyclohexane-1,2,4-triol
Bos taurus
-
IC50 is 5 mM
54
(-)-(1S,2R,4S,6R)-6-(2-phenylethyl)amino-2,4-dihydroxycyclohexyl 1-methylphosphonate
Bos taurus
-
IC50 is above 54 mM
6
(-)-(1S,2R,4S,6R)-6-(2-phenylethyl)aminocyclohexane-1,2,4-triol
Bos taurus
-
IC50 is 6 mM
10
(-)-(1S,2R,4S,6R)-6-(3-aminopropyloxy)cyclohexane-1,2,4-triol
Bos taurus
-
IC50 is 10 mM
50
(-)-(1S,2R,4S,6R)-6-aminocyclohexane-1,2,4-triol
1.3
(-)-(1S,2R,4S,6R)-6-hexylamino-2,4-dihydroxycyclohexyl 1-methylphosphonate
Bos taurus
-
IC50 is 1.3 mM
10
(-)-(1S,2R,4S,6R)-6-hexyloxycyclohexane-1,2,4-triol
Bos taurus
-
IC50 is about 10 mM
8
(-)-(1S,2R,4S,6R)-6-methylamino-2,4-dihydroxycyclohexyl 1-methylphosphonate
Bos taurus
-
IC50 is above 8 mM
4
(-)-(1S,2R,4S,6R)-6-octylaminocyclohexane-1,2,4-triol
Bos taurus
-
IC50 is 4 mM
9
(-)-(1S,2R,4S,6R)-6-[N-(4-phenylbutyl)-N-(2-aminoethyl)-amino]cyclohexane-1,2,4-triol [hydrochloride salt]
Bos taurus
-
IC50 is 9 mM
0.00007
(1R,2R,4R,6R)-2,4-dihydroxy-6-[4-(2-hydroxyphenyl)butoxy]cyclohexyl phosphate
Homo sapiens
-
-
0.0002
(phenylmethanediyl)bis(phosphonic acid)
0.0008
2,3,7-trihydroxycyclohepta-2,4,6-trien-1-one
Homo sapiens
-
-
0.7 - 290
Li+
30
LiCl
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.7
-
purified enzyme
3.3
-
purified enzyme, pH 8.0, 37C
4.37
-
total cell extract, at 37C, using sodium diphosphate supplemented with MgCl2 as a substrate
375.6
-
after 85.94fold purification, at 37C, using sodium diphosphate supplemented with MgCl2 as a substrate
additional information