Cloned (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
- |
Thermotoga maritima |
expressed in Escherichia coli | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, best crystals in the presence of Zn2+ and phosphate | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
D-fructose 1,6-bisphosphate | 85°C | Methanocaldococcus jannaschii | |
0.07 | - |
myo-inositol 1-phosphate | 37°C | Escherichia coli | |
0.091 | - |
myo-inositol 1-phosphate | 85°C | Methanocaldococcus jannaschii | |
0.11 | - |
myo-inositol 1-phosphate | 85°C | Archaeoglobus fulgidus | |
0.13 | - |
myo-inositol 1-phosphate | 95°C | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate + H2O | Archaeoglobus fulgidus | very effective substrate | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | Thermotoga maritima | very effective substrate | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | Methanocaldococcus jannaschii | very effective substrate | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | Thermotoga maritima TM1415 | very effective substrate | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | Archaeoglobus fulgidus AF2372 | very effective substrate | D-fructose 6-phosphate + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | Escherichia coli | - |
myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | Archaeoglobus fulgidus | organism do not contain lipids with inositol head group, involved in the response to thermal stress | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | Thermotoga maritima | organism do not contain lipids with inositol head group, involved in the response to thermal stress | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | Methanocaldococcus jannaschii | organism do not contain lipids with inositol head group, involved in the response to thermal stress | myo-inositol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Archaeoglobus fulgidus AF2372 | - |
- |
- |
Escherichia coli | - |
- |
- |
Methanocaldococcus jannaschii | Q57573 | - |
- |
Thermotoga maritima | - |
- |
- |
Thermotoga maritima TM1415 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
- |
Thermotoga maritima |
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-AMP + H2O | 600% of the rate with inositol 1-phosphate | Methanocaldococcus jannaschii | adenosine + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | very effective substrate | Archaeoglobus fulgidus | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | very effective substrate | Thermotoga maritima | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | very effective substrate | Methanocaldococcus jannaschii | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | very effective substrate | Thermotoga maritima TM1415 | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | very effective substrate | Archaeoglobus fulgidus AF2372 | 4-nitrophenol + phosphate | - |
? | |
alpha-D-glucose 1-phosphate + H2O | - |
Archaeoglobus fulgidus | alpha-D-glucose + phosphate | - |
? | |
alpha-D-glucose 1-phosphate + H2O | - |
Thermotoga maritima | alpha-D-glucose + phosphate | - |
? | |
alpha-D-glucose 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | alpha-D-glucose + phosphate | - |
? | |
alpha-D-glucose 1-phosphate + H2O | - |
Thermotoga maritima TM1415 | alpha-D-glucose + phosphate | - |
? | |
alpha-D-glucose 1-phosphate + H2O | - |
Archaeoglobus fulgidus AF2372 | alpha-D-glucose + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | very effective substrate | Archaeoglobus fulgidus | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | very effective substrate | Thermotoga maritima | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | very effective substrate | Methanocaldococcus jannaschii | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | very effective substrate | Thermotoga maritima TM1415 | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | very effective substrate | Archaeoglobus fulgidus AF2372 | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 2,6-bisphosphate + H2O | 16% of the rate with inositol 1-phosphate | Methanocaldococcus jannaschii | D-fructose 6-phosphate + phosphate | - |
? | |
glycerol 2-phosphate + H2O | - |
Methanocaldococcus jannaschii | glycerol + phosphate | - |
? | |
additional information | archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity | Archaeoglobus fulgidus | ? | - |
? | |
additional information | archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity | Thermotoga maritima | ? | - |
? | |
additional information | D-glucose 6-phosphate, D-fructose 6-phosphate, 5'-AMP and NAD+ are not hydrolyzed | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | traces of activity with glucose 1-phosphate, no activity with 4-nitrophenyl phosphate and fructose 1,6-bisphosphate | Escherichia coli | ? | - |
? | |
additional information | archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity | Thermotoga maritima TM1415 | ? | - |
? | |
additional information | archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity | Archaeoglobus fulgidus AF2372 | ? | - |
? | |
myo-inositol 1-phosphate + H2O | - |
Escherichia coli | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | - |
Archaeoglobus fulgidus | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | - |
Thermotoga maritima | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | organism do not contain lipids with inositol head group, involved in the response to thermal stress | Archaeoglobus fulgidus | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | organism do not contain lipids with inositol head group, involved in the response to thermal stress | Thermotoga maritima | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | organism do not contain lipids with inositol head group, involved in the response to thermal stress | Methanocaldococcus jannaschii | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | - |
Thermotoga maritima TM1415 | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | - |
Archaeoglobus fulgidus AF2372 | myo-inositol + phosphate | - |
? | |
myo-inositol 2-phosphate + H2O | 15% of the rate with inositol 1-phosphate | Methanocaldococcus jannaschii | myo-inositol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Archaeoglobus fulgidus |
dimer | crystal structure analysis | Methanocaldococcus jannaschii |
monomer | - |
Escherichia coli |
tetramer | - |
Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
More | archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
myo-inositol 1-phosphate | 50°C | Methanocaldococcus jannaschii | |
2.5 | - |
myo-inositol 1-phosphate | 85°C | Archaeoglobus fulgidus | |
2.7 | - |
D-fructose 1,6-bisphosphate | 50°C | Methanocaldococcus jannaschii | |
2.7 | - |
D-fructose 1,6-bisphosphate | 85°C | Archaeoglobus fulgidus | |
4.2 | - |
myo-inositol 1-phosphate | 85°C | Methanocaldococcus jannaschii | |
6.5 | - |
myo-inositol 1-phosphate | 37°C | Escherichia coli | |
7 | - |
D-fructose 1,6-bisphosphate | 85°C | Methanocaldococcus jannaschii | |
207 | - |
myo-inositol 1-phosphate | 95°C | Thermotoga maritima | |
268 | - |
D-fructose 1,6-bisphosphate | 95°C | Thermotoga maritima |