Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 | Rhizobium leguminosarum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | - |
Rhizobium leguminosarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
myo-inositol 1-phosphate | pH 7.8, 30°C | Rhizobium leguminosarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no activity at 3 mM | Rhizobium leguminosarum | |
Co2+ | partial activation of enzyme, 16.8% of the activity observed with Mg2+ | Rhizobium leguminosarum | |
Mg2+ | required, highest activity with 3 mM Mg2+, inhibitory at high concentrations, 50% inhibition at 100 mM | Rhizobium leguminosarum | |
Mn2+ | partial activation of enzyme, 22.7% of the activity observed with Mg2+ | Rhizobium leguminosarum | |
Zn2+ | partial activation of enzyme, 8.2% of the activity observed with Mg2+ | Rhizobium leguminosarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30350 | - |
calculated mass of the Escherichia coli expressed protein | Rhizobium leguminosarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol 1-phosphate + H2O | Rhizobium leguminosarum | reguglation of exopolysaccharide synthesis suggested | myo-inositol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium leguminosarum | - |
bv. trifolii | - |
Purification (Comment) | Organism |
---|---|
recombinant protein from Escherichia coli using His-tag | Rhizobium leguminosarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-AMP + H2O | 18% of the rate with inositol 1-phosphate | Rhizobium leguminosarum | adenosine + phosphate | - |
? | |
glycerol 2-phosphate + H2O | 64% of the rate with inositol 1-phosphate | Rhizobium leguminosarum | glycerol + phosphate | - |
? | |
additional information | traces of activity with glucose 1-phosphate and 4-nitrophenylphosphate, no activity with glucose 6-phosphate and 5'-AMP | Rhizobium leguminosarum | ? | - |
? | |
myo-inositol 1-phosphate + H2O | - |
Rhizobium leguminosarum | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | reguglation of exopolysaccharide synthesis suggested | Rhizobium leguminosarum | myo-inositol + phosphate | - |
? | |
myo-inositol 4-phosphate + H2O | - |
Rhizobium leguminosarum | myo-inositol + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Rhizobium leguminosarum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.35 | - |
Li+ | pH 7.8, 30°C | Rhizobium leguminosarum |