Cloned (Comment) | Organism |
---|---|
gene Rv2131c, expression of soluble N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
D40N | site-sirected mutagenesis, nearly inactive mutant concerning both inositol monophosphatase and fructose-1,6-bisphosphatase activities | Mycobacterium tuberculosis |
D94N | site-sirected mutagenesis, nearly inactive mutant concerning both inositol monophosphatase and fructose-1,6-bisphosphatase activities | Mycobacterium tuberculosis |
L71A | site-sirected mutagenesis, no decrease in catalytic activity, but the mutant enzyme is 12fold more resistant to inhibition by Li+ compared to the wild-type enzyme | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
D-myo-Inositol 1-phosphate | pH 9.3, 37°C, recombinant wild-type enzyme | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Mycobacterium tuberculosis | |
Mn2+ | can partially substitute for Mg2+ | Mycobacterium tuberculosis | |
additional information | no activity wth Co2+, Ni2+, Cu2+, Zn2+, and Ca2+, no effect by Na+ and K+ | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, about, recombinant wild-type enzyme, SDS-PAGE | Mycobacterium tuberculosis |
62500 | - |
native PAGE, recombinant wild-type enzyme | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
gene Rv2131c | - |
Mycobacterium tuberculosis H37Rv | - |
gene Rv2131c | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
myo-inositol phosphate + H2O = myo-inositol + phosphate | enzyme is both inositol monophosphatase and fructose-1,6-bisphosphatase | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-myo-inositol 1-phosphate + H2O | - |
Mycobacterium tuberculosis | D-myo-inositol + phosphate | - |
? | |
D-myo-inositol 1-phosphate + H2O | - |
Mycobacterium tuberculosis H37Rv | D-myo-inositol + phosphate | - |
? | |
additional information | substrate specificity, enzyme is both inositol monophosphatase and fructose-1,6-bisphosphatase | Mycobacterium tuberculosis | ? | - |
? | |
additional information | substrate specificity, enzyme is both inositol monophosphatase and fructose-1,6-bisphosphatase | Mycobacterium tuberculosis H37Rv | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, about, recombinant wild-type enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
IMPase | - |
Mycobacterium tuberculosis |
IMPase/FBPase | - |
Mycobacterium tuberculosis |
inositol monophosphatase | - |
Mycobacterium tuberculosis |
inositol monophosphatase/fructose-1,6-bisphosphatase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
62 | - |
- |
Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
2 min, loss of 95% activity | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
assay at | Mycobacterium tuberculosis |