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Literature summary for 3.1.3.25 extracted from

  • Gu, X.; Chen, M.; Shen, H.; Jiang, X.; Huang, Y.; Wang, H.
    Rv2131c gene product: an unconventional enzyme that is both inositol monophosphatase and fructose-1,6-bisphosphatase (2006), Biochem. Biophys. Res. Commun., 339, 897-904.
    View publication on PubMed
Search for more literature for 3.1.3.25

Cloned(Commentary)

Cloned (Comment) Organism
gene Rv2131c, expression of soluble N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
D40N site-sirected mutagenesis, nearly inactive mutant concerning both inositol monophosphatase and fructose-1,6-bisphosphatase activities Mycobacterium tuberculosis
D94N site-sirected mutagenesis, nearly inactive mutant concerning both inositol monophosphatase and fructose-1,6-bisphosphatase activities Mycobacterium tuberculosis
L71A site-sirected mutagenesis, no decrease in catalytic activity, but the mutant enzyme is 12fold more resistant to inhibition by Li+ compared to the wild-type enzyme Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
Li+
-
 Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 D-myo-Inositol 1-phosphate pH 9.3, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Mycobacterium tuberculosis
Mn2+ can partially substitute for Mg2+ Mycobacterium tuberculosis
additional information no activity wth Co2+, Ni2+, Cu2+, Zn2+, and Ca2+, no effect by Na+ and K+ Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, about, recombinant wild-type enzyme, SDS-PAGE Mycobacterium tuberculosis
62500
-
 native PAGE, recombinant wild-type enzyme Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
 gene Rv2131c
-
Mycobacterium tuberculosis H37Rv
-
 gene Rv2131c
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Mycobacterium tuberculosis

Reaction

Reaction Comment Organism Reaction ID
myo-inositol phosphate + H2O = myo-inositol + phosphate enzyme is both inositol monophosphatase and fructose-1,6-bisphosphatase Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-myo-inositol 1-phosphate + H2O
-
 Mycobacterium tuberculosis D-myo-inositol + phosphate
-
 ?
D-myo-inositol 1-phosphate + H2O
-
 Mycobacterium tuberculosis H37Rv D-myo-inositol + phosphate
-
 ?
additional information substrate specificity, enzyme is both inositol monophosphatase and fructose-1,6-bisphosphatase Mycobacterium tuberculosis ?
-
 ?
additional information substrate specificity, enzyme is both inositol monophosphatase and fructose-1,6-bisphosphatase Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, about, recombinant wild-type enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
IMPase
-
 Mycobacterium tuberculosis
IMPase/FBPase
-
 Mycobacterium tuberculosis
inositol monophosphatase
-
 Mycobacterium tuberculosis
inositol monophosphatase/fructose-1,6-bisphosphatase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
62
-
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 2 min, loss of 95% activity Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
 assay at Mycobacterium tuberculosis