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Literature summary for 3.1.3.25 extracted from
- Purification and biochemical characterization of Mycobacterium tuberculosis SuhB, an inositol monophosphatase involved in inositol biosynthesis (2002), Biochemistry, 41, 4392-4398.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D104N | activity almost completely abolished | Mycobacterium tuberculosis |
| D107N | activity almost completely abolished | Mycobacterium tuberculosis |
| D235N | activity almost completely abolished | Mycobacterium tuberculosis |
| E83D | activity almost completely abolished | Mycobacterium tuberculosis |
| L81A | 10fold more resistant towards Li+ inhibition compared to wild type enzyme | Mycobacterium tuberculosis |
| W234L | activity almost completely abolished | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Be2+ | 50% inhibition at 0.09 mM | Mycobacterium tuberculosis |  |
| Ca2+ | 50% inhibition at 0.4 mM | Mycobacterium tuberculosis | |
| Co2+ | 50% inhibition at 0.7 mM | Mycobacterium tuberculosis | |
| Cr2+ | 50% inhibition at 3 mM | Mycobacterium tuberculosis | |
| Cu2+ | 50% inhibition at 1 mM | Mycobacterium tuberculosis | |
| Li+ | 50% inhibition at 0.9 mM | Mycobacterium tuberculosis | |
| Mn2+ | 50% inhibition at 0.8 mM | Mycobacterium tuberculosis | |
| Ni2+ | 50% inhibition at 30 mM | Mycobacterium tuberculosis | |
| Zn2+ | 50% inhibition at 0.005 mM | Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.177 | - |
myo-inositol 1-phosphate | pH 8.5, 37°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 30% of the activity with Mg2+ | Mycobacterium tuberculosis | |
| Fe2+ | 30% of the activity with Mg2+ | Mycobacterium tuberculosis | |
| Mg2+ | required, highest activity 6-100 mM Mg2+, inhibitory above 100 mM | Mycobacterium tuberculosis | |
| Mn2+ | 13% of the activity with Mn2+ | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
calculated from amino acid sequence | Mycobacterium tuberculosis |
| 36000 | - |
3 * 36000, SDs-PAGE | Mycobacterium tuberculosis |
| 93000 | - |
native PAGE | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol 1-phosphate + H2O | Mycobacterium tuberculosis | synthesis of myo-inositol, that is a key substrate for synthesis of phosphatidylinositol | myo-inositol + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein from Escherichia coli using His-tag | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-AMP + H2O | - |
Mycobacterium tuberculosis | adenosine + phosphate | - |
? | |
| glucitol 6-phosphate + H2O | - |
Mycobacterium tuberculosis | glucitol + phosphate | - |
? | |
| glycerol 2-phosphate + H2O | - |
Mycobacterium tuberculosis | glycerol + phosphate | - |
? | |
| mannitol 1-phosphate + H2O | - |
Mycobacterium tuberculosis | mannitol + phosphate | - |
? | |
| myo-inositol 1-phosphate + H2O | - |
Mycobacterium tuberculosis | myo-inositol + phosphate | - |
? | |
| myo-inositol 1-phosphate + H2O | synthesis of myo-inositol, that is a key substrate for synthesis of phosphatidylinositol | Mycobacterium tuberculosis | myo-inositol + phosphate | - |
? | |
| myo-inositol 2-phosphate + H2O | - |
Mycobacterium tuberculosis | myo-inositol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 36000, SDs-PAGE | Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Mycobacterium tuberculosis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 100 | - |
Mycobacterium tuberculosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
50% of activity lost within 1 min, Mg2+ stabilizes activity for short periods | Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.6 | - |
myo-inositol 1-phosphate | pH 8.5, 37°C | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Mycobacterium tuberculosis |
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