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Literature summary for 3.1.3.25 extracted from

  • Fujita, S.; Ohnishi, T.; Okuda, S.; Kobayashi, R.; Fukuno, S.; Furuta, D.; Kikuchi, T.; Yoshikawa, T.; Fujita, N.
    In silico study on the substrate binding manner in human myo-inositol monophosphatase 2 (2011), J. Mol. Model., 17, 2559-2567.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for catalytic activity, the phosphate moiety coordinates three Mg2+ ions, and the inositol ring forms hydrogen bonds with the amino acids conserved in the family. 3D models of three- and two-Mg2+-ion bound myo-inositol monophosphatase 2, docking and binding structure analysis, detailed overview Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32400
-
2 * 32400, about, sequence calculation Homo sapiens
64900
-
IMPA2, analytical ultracentrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol 1-phosphate + H2O Homo sapiens
-
myo-inositol + phosphate
-
?
myo-inositol 3-phosphate + H2O Homo sapiens
-
myo-inositol + phosphate
-
?
myo-inositol 4-phosphate + H2O Homo sapiens
-
myo-inositol + phosphate
-
?
myo-inositol 5-phosphate + H2O Homo sapiens
-
myo-inositol + phosphate
-
?
myo-inositol 6-phosphate + H2O Homo sapiens
-
myo-inositol + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
gene IMPA2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inositol 4-phosphate + H2O the inositol ring docks to the enzyme by forming four hydrogen bonds: the 2-OH group with Asp104, the 3-OH with Asp231, the 5-OH group with Thr106, and the 6-OH with the carbonyl group of main chain of Gly205 Homo sapiens myo-inositol + phosphate
-
?
additional information substrate binding structure of IMPA2, docking study on other myo-inositol monophosphates, overview. Common binding patterns: the non-bridging oxygen atom O9 coordinated Mg2+-3 and O8 coordinated both Mg2+-1 and Mg2+-2. The bridging oxygen atom coordinated Mg2+-2. No activity with inositol 2-phosphate Homo sapiens ?
-
?
myo-inositol 1-phosphate + H2O
-
Homo sapiens myo-inositol + phosphate
-
?
myo-inositol 3-phosphate + H2O
-
Homo sapiens myo-inositol + phosphate
-
?
myo-inositol 3-phosphate + H2O the substrate shows a similar binding structure as inositol 5-phosphate Homo sapiens myo-inositol + phosphate
-
?
myo-inositol 4-phosphate + H2O
-
Homo sapiens myo-inositol + phosphate
-
?
myo-inositol 5-phosphate + H2O
-
Homo sapiens myo-inositol + phosphate
-
?
myo-inositol 5-phosphate + H2O myo-inositol 5-phosphate forms an electrostatic interaction between the metal ions and phosphate moiety, and the 2-OH group forms a hydrogen bond with the carbonyl group of Gln224, the 3-OH with the nitrogen atom of side chain of Gln224, and 4-OH with Asp231. The non-bridging oxygen O9 interacts with the 4-OH group, and the bridging oxygen O1 with Mg2+-2 Homo sapiens myo-inositol + phosphate
-
?
myo-inositol 6-phosphate + H2O
-
Homo sapiens myo-inositol + phosphate
-
?
myo-inositol 6-phosphate + H2O the substrate shows a similar binding structure as inositol 5-phosphate Homo sapiens myo-inositol + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32400, about, sequence calculation Homo sapiens
More IMPA2 structure analysis using IMPA1 crystal structure as a template, overview Homo sapiens

Synonyms

Synonyms Comment Organism
IMPA2
-
Homo sapiens
myo-inositol monophosphatase 2
-
Homo sapiens