Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, in presence of Ca2+, Zn2+ and substrate or product | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Methanocaldococcus jannaschii | |
Li+ | poor inhibition | Methanocaldococcus jannaschii | |
Zn2+ | - |
Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | two ions bound per subunit | Methanocaldococcus jannaschii | |
Mn2+ | three ions bound per subunit | Methanocaldococcus jannaschii | |
Zn2+ | three ions bound per subunit | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol 1-phosphate + H2O | Methanocaldococcus jannaschii | does not contain inositol-containing lipids, involved in biosynthesis of di-myo-inositol-1,1'-phosphate, that serves as osmolyte in hypertermophilic archea | myo-inositol + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q57573 | strain MJ109 | - |
Methanocaldococcus jannaschii DSM 2661 | Q57573 | strain MJ109 | - |
Purification (Comment) | Organism |
---|---|
recombinant protein from Escherichia coli | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-AMP + H2O | 600% of the rate with inositol 1-phosphate | Methanocaldococcus jannaschii | adenosine + phosphate | - |
? | |
2'-AMP + H2O | 600% of the rate with inositol 1-phosphate | Methanocaldococcus jannaschii DSM 2661 | adenosine + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | very effective substrate | Methanocaldococcus jannaschii | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | very effective substrate | Methanocaldococcus jannaschii DSM 2661 | 4-nitrophenol + phosphate | - |
? | |
alpha-D-glucose 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | alpha-D-glucose + phosphate | - |
? | |
alpha-D-glucose 1-phosphate + H2O | - |
Methanocaldococcus jannaschii DSM 2661 | alpha-D-glucose + phosphate | - |
? | |
D-fructose 1,6-bisphosphate + H2O | very effective substrate | Methanocaldococcus jannaschii | D-fructose 6-phosphate + phosphate | - |
? | |
D-fructose 2,6-bisphosphate + H2O | 16% of the rate with inositol 1-phosphate | Methanocaldococcus jannaschii | D-fructose 6-phosphate + phosphate | - |
? | |
glycerol 2-phosphate + H2O | - |
Methanocaldococcus jannaschii | glycerol + phosphate | - |
? | |
glycerol 2-phosphate + H2O | - |
Methanocaldococcus jannaschii DSM 2661 | glycerol + phosphate | - |
? | |
additional information | D-glucose 6-phosphate, D-fructose 6-phosphate, 5'-AMP and NAD+ are not hydrolyzed | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | D-glucose 6-phosphate, D-fructose 6-phosphate, 5'-AMP and NAD+ are not hydrolyzed | Methanocaldococcus jannaschii DSM 2661 | ? | - |
? | |
myo-inositol 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | myo-inositol + phosphate | - |
? | |
myo-inositol 1-phosphate + H2O | does not contain inositol-containing lipids, involved in biosynthesis of di-myo-inositol-1,1'-phosphate, that serves as osmolyte in hypertermophilic archea | Methanocaldococcus jannaschii | myo-inositol + phosphate | - |
? | |
myo-inositol 2-phosphate + H2O | 15% of the rate with inositol 1-phosphate | Methanocaldococcus jannaschii | myo-inositol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystal structure analysis | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
More | archeal enzyme displays inositol monophosphatase as well as fructose 1,6-bisphosphatase activity | Methanocaldococcus jannaschii |