EC Number   |
|---|
    3.1.3.25 | compared of an in silico model of the IMPase-1/calbindin complex with the crystal structure of calcium binding protein S100B. Calbindin and S100B activate IMPase-1 in a similar mode |
| 3.1.3.25 | crystallized by vapor diffusion using hanging drops of 0.005 ml. The apoenzyme is crystallized in either 0.2 M ammonium nitrate and 30% PEG 3350 or 0.2 M dihydrogen ammonium phosphate and 30% PEG 3350 |
| 3.1.3.25 | hanging drop vapor diffusion method, in presence of Ca2+, Zn2+ and substrate or product |
| 3.1.3.25 | hanging drop vapor diffusion method, using 0.2 M MgCl2, 0.1 M Na/HEPES pH 8.0, 18% (w/v) poly(ethyleneglycol) 3350 |
| 3.1.3.25 | hanging drop vapour diffusion method with in 10-14% (v/v) 2-methyl-2,4-pentanediol, 0.1 M tri-sodium citrate, pH 5.6, and 0.2 M ammonium acetate |
| 3.1.3.25 | hanging drop vapour diffusion method, best crystals in the presence of Zn2+ and phosphate |
| 3.1.3.25 | MJ0109 with bound metal ions, Mg2+, Zn2+, or Mn2+, crystal structure analysis, PDB ID 1DK4 |
| 3.1.3.25 | purified recombinant enzyme in complex with Mg2+, room temperature, hanging drop vapour diffusion method, 0.002 ml protein solution containing 20 mg/ml protein in 20 mm Tris-HCl, pH 7.5, and 20 mM MgCl2, is mixed with 0.002 ml reservoir solution containing 0.1 M sodium acetate, 0.1 M sodium HEPES, pH 8.5, and 15% PEG 4000, X-ray diffraction structure determination and analysis at 1.4 A resolution |
| 3.1.3.25 | purified recombinant enzyme, sitting drop vapour diffusion method, 24 mg/ml protein in 10 mM Tris-HCl pH 8.0, 50 mM NaCl, 2 mM MgCl2, 5mM DTT is mixed with crystallization solution containing 0.2 M Li2SO4, 20% PEG 3350, 0.1 M HEPES, pH 7.0, method optimization, X-ray diffraction structure determination and analysis at 2.6 A resolution |
| 3.1.3.25 | sitting drop vapor diffusion method, using 40% (w/v) PEG 3350, 0.2 M magnesium formate, at 22°C |
