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Literature summary for 3.1.3.25 extracted from

  • Chen, L.; Roberts, M.F.
    Cloning and expression of the inositol monophosphatase gene from Methanococcus jannaschii and characterization of the enzyme (1998), Appl. Environ. Microbiol., 64, 2609-2615.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.25

Cloned(Commentary)

Cloned (Comment) Organism
expression of the MJ109 gene in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Methanocaldococcus jannaschii
Ca2+
-
 Methanocaldococcus jannaschii
Co2+
-
 Methanocaldococcus jannaschii
Cu2+
-
 Methanocaldococcus jannaschii
Li+ 250 mM inhibits about 65%, 1000 mM about 90% Methanocaldococcus jannaschii
Ni2+
-
 Methanocaldococcus jannaschii
Zn2+
-
 Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Li+ at 100 mM the activity is slightly enhanced Methanocaldococcus jannaschii
Mg2+
-
 Methanocaldococcus jannaschii
Mn2+
-
 Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, SDS-PAGE Methanocaldococcus jannaschii
55000
-
 gel filtration Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.3
-
-
 Methanocaldococcus jannaschii

Storage Stability

Storage Stability Organism
4°C, no loss of activity for 1 month Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-AMP + H2O
-
 Methanocaldococcus jannaschii adenosine + phosphate
-
 ?
4-nitrophenyl phosphate + H2O high rate of hydrolysis Methanocaldococcus jannaschii 4-nitrophenol + phosphate
-
 ?
glucose 1-phosphate + H2O high rate of hydrolysis Methanocaldococcus jannaschii ?
-
 ?
glycerol 2-phosphate + H2O
-
 Methanocaldococcus jannaschii glycerol + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 for 30 min 95% of activity remains Methanocaldococcus jannaschii