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Literature summary for 3.1.3.25 extracted from

  • Fu, J.; Peterson, K.; Guttieri, M.; Souza, E.; Raboy, V.
    Barley (Hordeum vulgare L.) inositol monophosphatase: gene structure and enzyme characteristics (2008), Plant Mol. Biol., 67, 629-642.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cDNA library screen, expression in Escherichia coli BL21(DE3), pCR4-TOPO, pET-28b vectors Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
DL-myo-inositol 1-phosphate
-
Hordeum vulgare
Li+ 0-4 mM LiCl, 3 mM Li+ inhibits activity by approximately 50%, inhibition increases with Li+ concentration Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0097
-
DL-myo-inositol 1-phosphate recombinant protein, 9.7 microM substrate, higher affinity as compared with reported Km values for other eukaryotic IMPases Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
SDS-PAGE Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare B3DFH0 cv. Harrington, wild-type and mutant
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, recombinant protein Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
leaf 4 week old plants Hordeum vulgare
-
root 4 week old plants Hordeum vulgare
-
seed early development stage, wild-type and mutant plants Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
initial characterization of the genomic structure including promoter elements, expression of the single copy IMP-1 gene, expression levels not significantly altered by abiotic stress treatments, catalytic properties well suited for a role in myo-inositol synthesis Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-myo-inositol 1-phosphate + H2O
-
Hordeum vulgare D-myo-inositol + phosphate
-
?
DL-myo-inositol 1-phosphate + H2O
-
Hordeum vulgare DL-myo-inositol + phosphate
-
?
L-myo-inositol 1-phosphate + H2O
-
Hordeum vulgare L-myo-inositol + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Hordeum vulgare

Synonyms

Synonyms Comment Organism
IMP-1 protein
-
Hordeum vulgare
IMPase
-
Hordeum vulgare

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.29
-
DL-myo-inositol 1-phosphate recombinant protein Hordeum vulgare

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.84
-
DL-myo-inositol 1-phosphate recombinant protein Hordeum vulgare