Information on EC 2.5.1.6 - methionine adenosyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.5.1.6
-
RECOMMENDED NAME
GeneOntology No.
methionine adenosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
adenosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2'-deoxymugineic acid phytosiderophore biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
Cysteine and methionine metabolism
-
-
ethylene biosynthesis I (plants)
-
-
L-methionine degradation I (to L-homocysteine)
-
-
Metabolic pathways
-
-
methionine metabolism
-
-
S-adenosyl-L-methionine biosynthesis
-
-
S-adenosyl-L-methionine cycle II
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:L-methionine S-adenosyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9012-52-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain (Biecheler) 1649
-
-
Manually annotated by BRENDA team
strain (Biecheler) 1649
-
-
Manually annotated by BRENDA team
a pathogenic piscine hemoflagellate, gene metK or MAT
SwissProt
Manually annotated by BRENDA team
gene metK
-
-
Manually annotated by BRENDA team
XL1-Blue strain
Uniprot
Manually annotated by BRENDA team
cat
-
-
Manually annotated by BRENDA team
strain GS115
-
-
Manually annotated by BRENDA team
strain GS115
-
-
Manually annotated by BRENDA team
strain LEM75
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
gene metK, fever group, Breinl and Madrid E strains
-
-
Manually annotated by BRENDA team
gene metK, strain Wilmington of the typhus group rickettsiae
-
-
Manually annotated by BRENDA team
induction by salinity stress
-
-
Manually annotated by BRENDA team
strain NRRL8165, gene SAM-s
Uniprot
Manually annotated by BRENDA team
KO-179 strain. Actinorhodin-overproducer
Uniprot
Manually annotated by BRENDA team
KO-179 strain. Actinorhodin-overproducer
Uniprot
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme plays a key role in the biogenesis of S-adenosyl-L-methionine
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-deoxy-ATP + L-methionine + H2O
?
show the reaction diagram
3'-deoxy-ATP + L-methionine + H2O
?
show the reaction diagram
ATP + (2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid
show the reaction diagram
ATP + (2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid
show the reaction diagram
ATP + (2S)-2-amino-4-(butylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(butylselanyl)butanoic acid
show the reaction diagram
ATP + (2S)-2-amino-4-(butylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(butylsulfanyl)butanoic acid
show the reaction diagram
27% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(ethylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(ethylselanyl)butanoic acid
show the reaction diagram
76% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(ethylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(ethylsulfanyl)butanoic acid
show the reaction diagram
84% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(prop-2-en-1-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(prop-2-en-1-ylselanyl)butanoic acid
show the reaction diagram
30% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(prop-2-en-1-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(prop-2-en-1-ylsulfanyl)butanoic acid
show the reaction diagram
27% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(prop-2-yn-1-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(prop-2-yn-1-ylselanyl)butanoic acid
show the reaction diagram
28% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(prop-2-yn-1-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(prop-2-yn-1-ylsulfanyl)butanoic acid
show the reaction diagram
36% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(propan-2-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(propan-2-ylselanyl)butanoic acid
show the reaction diagram
40% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(propan-2-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(propan-2-ylsulfanyl)butanoic acid
show the reaction diagram
53% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(propylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(propylselanyl)butanoic acid
show the reaction diagram
66% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(propylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(propylsulfanyl)butanoic acid
show the reaction diagram
44% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2-azido ethyl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-azido ethyl)sulfanyl]butanoic acid
show the reaction diagram
50% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2-methyl propyl)sulfanyl] butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-methyl propyl)sulfanyl]butanoic acid
show the reaction diagram
57% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2-methylprop-2-en-1-yl)selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(2-methylprop-2-en-1-yl)selanyl]butanoic acid
show the reaction diagram
46% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2-methylprop-2-en-1-yl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-methylprop-2-en-1-yl)sulfanyl]butanoic acid
show the reaction diagram
15% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2E)-but-2-en-1-ylsulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2E)-but-2-en-1-ylsulfanyl]butanoic acid
show the reaction diagram
27% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2E)-penta-2,4-dien-1-ylsulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2E)-penta-2,4-dien-1-ylsulfanyl]butanoic acid
show the reaction diagram
25% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(3-azido propyl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-azido propyl)sulfanyl]butanoic acid
show the reaction diagram
60% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(3-methyl butyl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-methyl butyl)sulfanyl] butanoic acid
show the reaction diagram
36% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(3-methylbut-2-en-1-yl)selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(3-methylbut-2-en-1-yl)selanyl]butanoic acid
show the reaction diagram
44% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(3-methylbut-2-en-1-yl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-methylbut-2-en-1-yl)sulfanyl]butanoic acid
show the reaction diagram
12% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(cyanomethyl) sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(cyanomethyl) sulfanyl]butanoic acid
show the reaction diagram
68% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(cyanomethyl)selanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(cyanomethyl) selanyl]butanoic acid
show the reaction diagram
12% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[[(2E)-4-aminobut-2-en-1-yl]selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[[(2E)-4-aminobut-2-en-1-yl]selanyl]butanoic acid
show the reaction diagram
38% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]selanyl]butanoic acid
show the reaction diagram
24% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]sulfanyl]butanoic acid
show the reaction diagram
28% of the turnover rate compared to L-methionine
-
-
?
ATP + D-methionine + H2O
S-adenosyl-D-methionine + phosphate + diphosphate
show the reaction diagram
-
-
-
?
ATP + L-ethionine + H2O
phosphate + diphosphate + S-adenosyl-L-ethionine
show the reaction diagram
-
-
-
?
ATP + L-ethionine + H2O
S-adenosyl-L-ethionine + phosphate + diphosphate
show the reaction diagram
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
show the reaction diagram
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
show the reaction diagram
ATP + L-methionine methyl ester + H2O
S-adenosyl-L-methionine methyl ester + phosphate + diphosphate
show the reaction diagram
-
-
-
?
ATP + L-selenomethionine + H2O
phosphate + diphosphate + Se-adenosyl-L-selenomethionine
show the reaction diagram
as active as L-methionine
-
-
?
ATP + methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
show the reaction diagram
CTP + L-methionine + H2O
S-cytosyl-L-methionine + phosphate + diphosphate
show the reaction diagram
-
-
-
?
GTP + L-methionine + H2O
S-guanosyl-L-methionine + phosphate + diphosphate
show the reaction diagram
-
-
-
?
ITP + L-methionine + H2O
?
show the reaction diagram
tripolyphosphate + H2O
diphosphate + phosphate
show the reaction diagram
UTP + L-methionine + H2O
S-urasyl-L-methionine + phosphate + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
show the reaction diagram
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
0.02 mM stimulates activity
Ni2+
0.02 mM stimulates activity
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S,4S)-amino-4,5-epoxypentanoic acid
-
1-(3-(2-ethoxyphenyl)ureidoacetyl)-4-(2-methyl-5-nitrophenyl)semicarbazide
-
binding to adenosyl region of the active site
1-(4-chloro-2-nitrophenyl)-3-(4-sulfamoylphenyl)-urea
-
binding to adenosyl region of the active site
1-aminocyclopentanecarboxylic acid
-
-
1-methyluric acid
10 mM, 43.4% inhibition
1-Methylxanthine
10 mM, 35.9% inhibition
2,6-Diaminopurine
10 mM, 29.3% inhibition
2,6-dichloropurine
10 mM, 35.5% inhibition
2-amino-6-carboxyethylmercaptopurine
10 mM, 31.9% inhibition
2-amino-6-chloropurine riboside
10 mM, 17.2% inhibition
2-amino-6-chloropurine-9-acetic acid
10 mM, 23.5% inhibition
2-Aminopurine
10 mM, 11.0% inhibition
2-Hydroxypurine
10 mM, 33.8% inhibition
3,7-dimethyluric acid
10 mM, 27.9% inhibition
3-morpholinosydnoniimide
-
loss of liver MAT activity in vivo
5-amino-L-norvaline
10-25% inhibition with 5 mM; 10-25% inhibition with 5 mM; 10-25% inhibition with 5 mM
5-azacytidine
-
0.2 mM leads to significant reduction of AdoMetS protein expression
6-benzyloxypurine
10 mM, 17.7% inhibition
6-bromopurine
10 mM, 31.4% inhibition
6-Chloropurine
10 mM, 31.4% inhibition
6-Chloropurine riboside
10 mM, 17.1% inhibition
6-Cyanopurine
10 mM, 24.2% inhibition
6-dimethylallylaminopurine riboside
10 mM, 41.6% inhibition
6-Dimethylaminopurine
10 mM, 28.0% inhibition
6-Mercaptopurine
10 mM, 40.1% inhibition
6-mercaptopurine riboside
10 mM, 30.0% inhibition
6-propoxypurine
10 mM, 27.9% inhibition
7-hydroxypropyl theophylline
10 mM, 16.1% inhibition
7-Methyluric acid
10 mM, 11.4% inhibition
7-methylxanthine
10 mM, 36.3% inhibition
8-aza-2,6-diaminopurine
10 mM, 40.0% inhibition
8-Azaguanine
; 10 mM, 81.7% inhibition
8-chlorotheophylline
10 mM, 7.0% inhibition
Adenyl-5'-ylimidodiphosphate
-
competitive with ATP
ADP
35-50% inhibition with 5 mM; 35-50% inhibition with 5 mM; 35-50% inhibition with 5 mM
alpha,beta-methylene-adenosine tetraphosphate
-
-
alpha,beta-methylene-ATP
-
-
alpha-methyl-DL-methionine
10 mM, 18.8% inhibition
AMP
-
causes complete inactivation of the enzyme
Azathioprine
; 10 mM, 75.5% inhibition
Ba2+
-
70.70% residual activity at 5 mM
bacterial lipopolysaccharide
beta,gamma-methylene-ATP
-
-
Br-
-
93.33% residual activity at 5 mM
Ca2+
-
86.36% residual activity at 5 mM
carbon tetrachloride
-
depletion of glutathione levels reduces MAT I/III activities in vivo
CH3COO-
-
92.25% residual activity at 5 mM
Cl-
-
85.27% residual activity at 5 mM
cycloleucine
diimidotriphosphate
-
mechanism
Dimethylsulfoxide
diphosphate
DL-2-Amino-trans-4-hexenoic acid
-
-
ethanol
25 mM ethanol substantially decreases the enzymatic activity of MAT II
Ethionine
32-38% inhibition with 5 mM; 32-38% inhibition with 5 mM; 32-38% inhibition with 5 mM
F-
-
88.84% residual activity at 5 mM
Fe2+
-
59.22% residual activity at 5 mM
Fumarylacetoacetate
-
reduces MAT I/III activity
glycerol
-
inhibits kidney isoenzyme gamma
GSH
-
causes complete inactivation of the enzyme
hydrogen peroxide
I-
-
87.91% residual activity at 5 mM
L-2-Amino-4-hexynoic acid
-
-
L-2-Amino-4-methoxy-cis-but-3-enoic acid
L-2-Amino-4-methylthio-cis-but-3-enoic acid
-
-
L-buthionine-(S,R)-sulfoximine
L-ethioninamide
10 mM, 23.9% inhibition
L-ethionine
L-methionine
L-methionine methyl ester
10 mM, 17.7% inhibition
L-Methionine sulfone
10 mM, 9.2% inhibition
L-methionine sulfoxide
10 mM, 4.0% inhibition
L-methionine sulfoximine
10 mM, 12.6% inhibition
L-Penicillamine
10 mM, 15.0% inhibition
Li+
-
81.40% residual activity at 5 mM
methanol
2.4% methanol depresses methionine adenosyltransferase specific activity, this effect is not observed with 0.8% methanol
methylthio propionaldehyde
10 mM, 18.4% inhibition
methylthioadenosine
Mg2+
-
inhibitory above 8.5 mM
N-ethylmaleimide
-
time-dependent inactivation of both MAT activities
nitric oxide
nitrosoglutathione
-
reversibly inhibits the isozyme MAT1 via NO binding to Cys114, no inhibition of isozymes MAT2 and MAT3, molecular mechanism for S-nitrosylation of the enzyme
O-methylguanine
10 mM, 60.3% inhibition
p-chloromercuribenzoate
phosphate
putrescine
15-25% inhibition with 5 mM; 15-25% inhibition with 5 mM; 15-25% inhibition with 5 mM
S-adenosyl-L-ethionine
-
-
S-adenosyl-L-homocysteine
S-adenosyl-L-methionine
-
feedback inhibition of isozyme MAT II
S-adenosylmethionine
S-carbamylcysteine
-
competitive with methionine
S-nitrosoglutathione
S-nitrosoglutathione monoethyl ester
-
inactivates
S-nitrosylated glutathione
-
rapid and dose-dependent loss of enzymatic activity of MAT I/III
S-Trifluoromethyl-L-homocysteine
-
-
seleno-L-methionine
-
-
SIN-1
-
rapid and dose-dependent loss of enzymatic activity of MAT I/III
Sodium diphosphate
-
-
spermidine
15-34% inhibition with 5 mM; 15-34% inhibition with 5 mM; 15-34% inhibition with 5 mM
spermine
30-40% inhibition with 5 mM; 30-40% inhibition with 5 mM; 30-40% inhibition with 5 mM
Tetrapolyphosphate
tripolyphosphate
Uric acid
10 mM, 45.6% inhibition
xanthine
10 mM, 35.4% inhibition
Zn2+
-
22.17% residual activity at 5 mM
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
tripolyphosphatase activity stimulated by preincubation with ATP and methionine
cycloheximide
-
cycloheximide inhibition of protein synthesis increases isozyme MAT2 mRNA level in the logarithmic phase only, an indication that the gene is regulated in promastigotes at the posttranscriptional level by protein factors that targets the transcript for degradation
Dimethylsulfoxide
dithiothreitol
-
required for alpha and beta isoenzymes activity, not required for gamma enzyme
E2F1
-
present continually in the MAT2A promoter during liver regeneration
-
E2F3
-
present continually in the MAT2A promoter during liver regeneration
-
E2F4
-
present continually in the MAT2A promoter during liver regeneration
-
Epidermal growth factor
forskolin
-
induction of enzyme in organ cell culture
glycerol
-
activates rat liver isoenzymes alpha and beta
insulin-like growth factor 1
isoproterenol
-
induction of enzyme in organ cell culture
L-methionine
17% increase is observed at 60 mM L-methionine; 17% increase of activity is observed at 60 mM L-methionine
leptin
methanol
-
53.26% increased yield of S-adenosyl-L-methionine at 1.0% methanol
methionine
-
stimulation of activity demonstrated in vivo. Tripolyphosphatase activity stimulated by preincubation with ATP and methionine
n-heptane
-
53.26% increased yield of S-adenosyl-L-methionine at 1.0% n-heptane
norepinephrine
-
induction of enzyme in organ cell culture
S-adenosylmethionine
SH-reagents
-
liver enzyme: requirement, tumor enzyme: no requirement
-
sorbitol
-
53.26% increased yield of S-adenosyl-L-methionine at 1.2% sorbitol
Sp1
-
binding of Sp1 to the MAT2A promoter is essential for the transcriptional up-regulation of the gene
-
tripolyphosphates
-
activation or inhibition, depending on isoenzyme, S-adenosylmethionine and tripolyphosphate concentration
Tumor necrosis factor alpha
-
induces expression of variant 1 (but not variant 2) MAT2beta
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002 - 31
ATP
3.5
D-methionine
-
-
1.4
ITP
-
pH 8.0, 58C
0.0056 - 0.74
L-ethionine
0.0023 - 3.3
L-methionine
2.6
L-methionine methyl esther
-
-
0.0083 - 1.12
methionine
0.006 - 0.007
Mg2+
0.3
MgATP2-
-
-
0.0013 - 0.026
tripolyphosphate
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000883 - 7.5
ATP
0.039 - 0.05
L-ethionine
0.0343 - 7.5
L-methionine
0.000000367 - 0.583
S-adenosylmethionine
0.000000217 - 0.125
tripolyphosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.43 - 65.2
ATP
6.95 - 7.12
L-ethionine
14.92 - 242
L-methionine
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.7
8-Azaguanine
37C, pH 8.2
0.8
ATP
-
noncompetitive inhibition at 0.5-5 mM ATP concentrations
3.7
Azathioprine
37C, pH 8.2
3.6 - 17
cycloleucine
0.4
D-methionine
-
-
0.000002
diimidotriphosphate
-
pH 8.0
0.3 - 0.35
diphosphate
0.71
DL-Ethionine
-
-
0.0031 - 0.0063
L-2-Amino-4-methoxy-cis-but-3-enoic acid
0.0057 - 0.021
L-2-Amino-4-methylthio-cis-but-3-enoic acid
250
Na+
-
in presence of Mg2+
0.05 - 4
S-adenosylmethionine
0.51
seleno-L-methionine
-
-
1.6
Sodium diphosphate
-
-
0.18
Tetrapolyphosphate
-
-
0.035 - 0.25
tripolyphosphate
additional information
additional information
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000019
1-(3-(2-ethoxyphenyl)ureidoacetyl)-4-(2-methyl-5-nitrophenyl)semicarbazide
Escherichia coli
-
pH 8.0, 25C
0.0000069
1-(4-chloro-2-nitrophenyl)-3-(4-sulfamoylphenyl)-urea
Escherichia coli
-
pH 8.0, 25C
0.287 - 0.748
Cu2+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0002
-
activity Peak II, adult erythrocytes
0.001
-
brain
0.0011
-
muscle
0.0041
-
pancreas
0.0042
-
intestine
0.0061
-
kidney
0.039
-
MAT I
0.0431
-
liver
0.058
-
tripolyphosphase activity
0.065
-
S-adenosylmethionine synthetase B
0.096
-
glutathione/glutathione disulfide-refolded MAT III
0.098
-
MAT III
0.1
-
glutathione/glutathione disulfide-refolded MAT I
0.109
purified recombinant His-tagged isozyme SAMS1
0.11
-
dithiothreitol-refolded MAT III
0.138
-
S-adenosylmethionine synthetase A
0.2
-
S-adenosylmethionine synthesis activity
0.28
-
low-MW isozyme
0.94
-
pH 8.0, 37C
3
-
beta isoenzyme from liver
4.61
-
high-MW isozyme
6.42
-
crude extract, at 35C
7.5
-
alpha isoenzyme from liver
11
-
isoenzyme II
12.2
-
-
12.4
-
-
18.7
-
isoenzyme I
23.83
-
after 3.71fold purification, at 35C
32.75
-
beta form from liver
39
-
gamma isoenzyme from liver
66
-
mutant D121N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
78.24
-
alpha form from liver
108
-
mutant D166N, pH 8.0; mutant D19N, presence of 0.05 mM S-adenosyl methionine, pH 8.0; mutant D282N, pH 8.0
138
-
mutant D249N, pH 8.0
210
-
mutant H17A, presence of 0.05 mM S-adenosyl methionine, pH 8.0
216
-
pH 7.0, 37C
222
-
wild-type, pH 8.0
240
-
mutant D282N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
252
-
mutant K256A, pH 8.0; mutant K256A, presence of 0.05 mM S-adenosyl methionine, pH 8.0
318
-
mutant K280A, pH 8.0
330
-
mutant D249N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
720
-
mutant K280A, presence of 0.05 mM S-adenosyl methionine, pH 8.0
792
-
mutant D166N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
960
-
mutant H17N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
3000
-
wild-type, presence of 0.05 mM S-adenosyl methionine, pH 8.0
4400
-
pH 8.2, 37C
31500
mutant C61S, presence of glutathione
32700
mutant C35S, presence of glutathione
69070
mutant C61S, presence of dithiothreitol
94470
mutant C35S, presence of dithiothreitol
95800
wild-type, presence of glutathione
110000
wild-type, presence of dithiothreitol
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.3
three isoenzymes; three isoenzymes; three isoenzymes
7
-
assay at
7.5 - 9.5
-
glutathione/glutathione disulfide-refolded MAT I