Cloned (Comment) | Organism |
---|---|
His6-tagged fusion protein expressed in Escherichia coli | Leishmania infantum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Leishmania infantum | |
diphosphate | inhibition for S-adenosylmethionine and L-methonine | Leishmania infantum | |
S-adenosyl-L-ethionine | - |
Leishmania infantum | |
S-adenosyl-L-homocysteine | - |
Leishmania infantum | |
S-adenosylmethionine | more than 50% inhibition at 1 mM | Leishmania infantum | |
tripolyphosphate | competitive with ATP; non competitive with L-methionine | Leishmania infantum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
native MAT-II, gel filtration | Leishmania infantum |
249000 | - |
recombinant MAT-II, gel filtration | Leishmania infantum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania infantum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant MAT II purified by a method that includes Ni-agarose bead affinity capture. Alternative method includes Triton X-100 and 8 mM urea buffered extraction of inclusion body phase and dialysis | Leishmania infantum |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Leishmania infantum | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Leishmania infantum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000883 | - |
ATP | - |
Leishmania infantum | |
0.0000883 | - |
S-adenosylmethionine | - |
Leishmania infantum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
tripolyphosphate | inhibition with respect to L-methionine | Leishmania infantum | |
0.25 | - |
tripolyphosphate | inhibition with respect to ATP | Leishmania infantum | |
0.3 | - |
diphosphate | inhibition with respect to ATP | Leishmania infantum | |
0.35 | - |
diphosphate | inhibition with respect to L-methionine | Leishmania infantum | |
0.8 | - |
ATP | noncompetitive inhibition at 0.5-5 mM ATP concentrations | Leishmania infantum | |
4 | - |
S-adenosylmethionine | noncompetitive inhibition at 0.5-5 mM L-methionine concentrations | Leishmania infantum |