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Literature summary for 2.5.1.6 extracted from

  • del Pino, M.M.; Corrales, F.J.; Mato, J.M.
    Hysteretic behavior of methionine adenosyltransferase III. Methionine switches between two conformations of the enzyme with different specific activity (2000), J. Biol. Chem., 275, 23476-23482.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP tripolyphosphatase activity stimulated by preincubation with ATP and methionine Rattus norvegicus
methionine stimulation of activity demonstrated in vivo. Tripolyphosphatase activity stimulated by preincubation with ATP and methionine Rattus norvegicus
additional information no effect with ADP and methionine or S-adenosylmethionine Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ATP ATP and methionine act as a switch between two different MAT III isoforms Rattus norvegicus
L-methionine ATP and methionine act as a switch between two different MAT III isoforms Rattus norvegicus
nitric oxide two MAT III isoforms, one with low tripolyphosphatase activity that is insensitive to NO and another with high tripolyphosphatase activity that is inhibited by NO Rattus norvegicus
S-nitrosoglutathione inhibits S-adenosylmethionine sinthetase activity Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + H2O Rattus norvegicus two reaction steps: S-adenosylmethionine synthesis and tripolyphosphate hydrolysis. Tripolyphosphate hydrolysis is the rate determining reaction S-adenosyl-L-methionine + phosphate + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
two MAT III isoforms
-

Purification (Commentary)

Purification (Comment) Organism
method that includes DEAE-Sepharose, phenyl-Sepharose and blue-Sepharose chromatographies and ultrafiltration Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O mechanism Rattus norvegicus S-adenosyl-L-methionine + phosphate + diphosphate
-
?
ATP + L-methionine + H2O two reaction steps: S-adenosylmethionine synthesis and tripolyphosphate hydrolysis. Tripolyphosphate hydrolysis is the rate determining reaction Rattus norvegicus S-adenosyl-L-methionine + phosphate + diphosphate
-
?
tripolyphosphate + H2O
-
Rattus norvegicus diphosphate + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.125
-
tripolyphosphate tripolyphosphatase activity Rattus norvegicus
0.583
-
S-adenosylmethionine S-adenosylmethionine synthesis Rattus norvegicus