Any feedback?
Literature summary for 2.5.1.6 extracted from
- Isozymes of S-adenosylmethionine synthetase from rat liver: isolation and characterization (1986), J. Biochem., 100, 67-75.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Dimethylsulfoxide | activates the beta isozyme from liver 13-15fold | Rattus norvegicus |  |
| Dimethylsulfoxide | weak activation of the alpha isozyme from liver | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Dimethylsulfoxide | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
ATP | beta form from liver | Rattus norvegicus | |
| 0.005 | - |
ATP | alpha form from liver | Rattus norvegicus | |
| 0.006 | - |
Mg2+ | alpha form from liver | Rattus norvegicus | |
| 0.007 | - |
Mg2+ | beta form from liver | Rattus norvegicus | |
| 0.017 | - |
L-methionine | alpha form from liver | Rattus norvegicus | |
| 0.5 | - |
methionine | beta form from liver | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
2 * 48000, beta isoenzyme from liver, SDS-PAGE | Rattus norvegicus |
| 48000 | - |
4 * 48000, alpha isoenzyme from liver, SDS-PAGE | Rattus norvegicus |
| 100000 | - |
beta isoenzyme from liver, gel filtration | Rattus norvegicus |
| 200000 | - |
alpha isoenzyme from liver, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| alpha isozyme from liver using immunoaffinity chromatography | Rattus norvegicus |
| beta isoenzyme from liver using several chromatographic steps | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | two isofunctional forms with different Km values | Rattus norvegicus | - |
| liver | alpha and beta isozymes | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 32.75 | - |
beta form from liver | Rattus norvegicus |
| 78.24 | - |
alpha form from liver | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxy-ATP + L-methionine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3'-deoxy-ATP + L-methionine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| ATP + L-methionine + H2O | - |
Rattus norvegicus | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
| ATP + methionine + H2O | - |
Rattus norvegicus | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 48000, beta isoenzyme from liver, SDS-PAGE | Rattus norvegicus |
| tetramer | 4 * 48000, alpha isoenzyme from liver, SDS-PAGE | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
