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Literature summary for 2.5.1.6 extracted from
- Characterization of S-adenosylmethionine synthetase from Streptomyces avermitilis NRRL8165 and its effect on antibiotic production (2006), Enzyme Microb. Technol., 39, 466-473.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene SAM-s, DNA and amino acid sequence determination and analysis, a putative purine-rich ribosomal binding site is located 3 bp upstream of the GTG start codon, potential -35 and -10 consensus promoter sequences, expression of His-tagged enzyme in Escherichia coli strain XL-1 Blue, overexpression in Streptomyces avermitilis leads to 2fold increased synthesis of the antibiotic avermectin, overexpression in Streptomyces peucetius leads to 3.5fold increased synthesis of the antibiotic doxorubicin | Streptomyces avermitilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
L-methionine | pH 8.0, 37°C | Streptomyces avermitilis |  |
| 0.34 | - |
ATP | pH 8.0, 37°C | Streptomyces avermitilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | bound by G49 | Streptomyces avermitilis | |
| Mg2+ | the enzyme requires Mn2+ or Mg2+, which are equally active, bound by D17 and D289 | Streptomyces avermitilis | |
| Mn2+ | the enzyme requires Mn2+ or Mg2+, which are equally active | Streptomyces avermitilis | |
| additional information | Ag+, Ca2+, Fe2+, and Cu2+ cannot substitue for Mg2+ or Mn2+ | Streptomyces avermitilis | |
| Zn2+ | 66% of the activity with Mg2+ or Mn2+ | Streptomyces avermitilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43500 | - |
x * 43500, recombinant enzyme, SDS-PAGE | Streptomyces avermitilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + H2O | Streptomyces avermitilis | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
| ATP + L-methionine + H2O | Streptomyces avermitilis NRRL 8165 | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces avermitilis | Q3HW35 | gene SAM-s | - |
| Streptomyces avermitilis NRRL 8165 | Q3HW35 | gene SAM-s | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain XL-1 Blue by nickel affiniy chromatography | Streptomyces avermitilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Streptomyces avermitilis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Streptomyces avermitilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + H2O | - |
Streptomyces avermitilis | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
| ATP + L-methionine + H2O | - |
Streptomyces avermitilis NRRL 8165 | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43500, recombinant enzyme, SDS-PAGE | Streptomyces avermitilis |
| More | the enzyme contains conserved sequence motifs of SAM-s proteins, such as nanopeptide 276GGGAFSGKD284 for the P-loop for ATP binding site and metal binding site with 17D and 289D for Mg2+ and 49G for K+ | Streptomyces avermitilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S-adenosylmethionine synthetase | - |
Streptomyces avermitilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Streptomyces avermitilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | - |
Streptomyces avermitilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Streptomyces avermitilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | - |
Streptomyces avermitilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | the binding site contains the conserved nanopeptide 276GGGAFSGKD284 for the P-loop | Streptomyces avermitilis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptomyces avermitilis | sequence calculation | - |
4.82 |
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