Information on EC 1.2.1.3 - aldehyde dehydrogenase (NAD+)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY
1.2.1.3
-
RECOMMENDED NAME
GeneOntology No.
aldehyde dehydrogenase (NAD+)
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
an aldehyde + NAD+ + H2O = a carboxylate + NADH + H+
show the reaction diagram
mechanism
-
an aldehyde + NAD+ + H2O = a carboxylate + NADH + H+
show the reaction diagram
mechanism proceeds mainly through an enzyme*NAD+ binary complex, but at high concentrations of acetaldehyde a small degree of randomness is observed, the rate-limiting step of the dehydrogenase reaction occurs after ternary complex interconversion
-
an aldehyde + NAD+ + H2O = a carboxylate + NADH + H+
show the reaction diagram
compulsory ordered ternary-complex mechanism with NAD+ binding before acetaldehyde
-
an aldehyde + NAD+ + H2O = a carboxylate + NADH + H+
show the reaction diagram
sequential addition of both substrates prior to release of products
-
an aldehyde + NAD+ + H2O = a carboxylate + NADH + H+
show the reaction diagram
compulsory ordered mechanism
-
an aldehyde + NAD+ + H2O = a carboxylate + NADH + H+
show the reaction diagram
at low aldehyde concentrations the mechanism is ordered with NAD+ as the first substrate to bind, at high aldehyde concentrations the mechanism is complex
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
oxidation
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
reduction
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
alanine metabolism
-
-
alkane oxidation
-
-
Arginine and proline metabolism
-
-
aromatic biogenic amine degradation (bacteria)
-
-
Ascorbate and aldarate metabolism
-
-
beta-Alanine metabolism
-
-
Biosynthesis of antibiotics
-
-
Biosynthesis of secondary metabolites
-
-
Chloroalkane and chloroalkene degradation
-
-
conversions
-
-
dehydrodiconiferyl alcohol degradation
-
-
dimethylsulfoniopropanoate biosynthesis I (Wollastonia)
-
-
dimethylsulfoniopropanoate biosynthesis II (Spartina)
-
-
dopamine degradation
-
-
Entner Doudoroff pathway
-
-
Entner-Doudoroff pathway III (semi-phosphorylative)
-
-
ethanol degradation II
-
-
ethanol degradation IV
-
-
fatty acid alpha-oxidation I
-
-
fatty acid alpha-oxidation II
-
-
fatty acid alpha-oxidation III
-
-
Fatty acid degradation
-
-
Glycerolipid metabolism
-
-
Glycolysis / Gluconeogenesis
-
-
histamine degradation
-
-
Histidine metabolism
-
-
histidine metabolism
-
-
hypotaurine degradation
-
-
juvenile hormone III biosynthesis I
-
-
juvenile hormone III biosynthesis II
-
-
L-tryptophan degradation X (mammalian, via tryptamine)
-
-
Limonene and pinene degradation
-
-
Lysine degradation
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
NAD/NADP-NADH/NADPH mitochondrial interconversion (yeast)
-
-
noradrenaline and adrenaline degradation
-
-
octane oxidation
-
-
octane oxidation
-
-
oxidative ethanol degradation III
-
-
Pentose and glucuronate interconversions
-
-
phytol degradation
-
-
propanol degradation
-
-
putrescine degradation III
-
-
Pyruvate metabolism
-
-
serotonin degradation
-
-
Tryptophan metabolism
-
-
Valine, leucine and isoleucine degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
aldehyde:NAD+ oxidoreductase
Wide specificity, including oxidation of D-glucuronolactone to D-glucarate.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
AHD-M1
-
-
-
-
ALD1A1
-
isoform
ALDDH
-
-
-
-
aldehyde dehydrogenase
-
-
aldehyde dehydrogenase
-
-
aldehyde dehydrogenase
-
-
aldehyde dehydrogenase
-
-
aldehyde dehydrogenase
-
-
aldehyde dehydrogenase
Q4F895
-
aldehyde dehydrogenase
Rhodococcus erythropolis UPV-1
Q4F895
-
-
aldehyde dehydrogenase
-
-
aldehyde dehydrogenase
Q402C7
-
aldehyde dehydrogenase
Sphingomonas sp. 14DN61
Q402C7
-
-
aldehyde dehydrogenase (NAD)
-
-
aldehyde dehydrogenase 1
-
-
aldehyde dehydrogenase 1A1
-
-
aldehyde dehydrogenase 1A1
-
isoform
aldehyde dehydrogenase 1A1
-
-
aldehyde dehydrogenase 1B1
-
isoform
aldehyde dehydrogenase 2
-
-
aldehyde dehydrogenase 2
P47738
-
aldehyde dehydrogenase 2
-
-
aldehyde dehydrogenase 3A1
-
-
aldehyde dehydrogenase 7A1
-
-
aldehyde dehydrogenase class 1
-
-
aldehyde dehydrogenase type 2
-
-
Aldehyde dehydrogenase [NAD+]
-
-
-
-
Aldehyde dehydrogenase, cytosolic
-
-
-
-
Aldehyde dehydrogenase, microsomal
-
-
-
-
aldehyde dehydrogenase-2
-
-
aldehyde dehydrogenase-2
-
-
aldehyde dehydrogenase7
Q9FPK6
-
aldehyde:NAD+ oxidoreductase
-
-
-
-
ALDH
Klebsiella pneumoniae DSM 2026
A6T782
-
-
ALDH
-
-
ALDH
Rhodococcus erythropolis UPV-1
Q4F895
-
-
ALDH
Sulfolobus tokodaii 7
-
-
-
ALDH 2
-
-
ALDH I
-
-
ALDH II
-
-
ALDH-2
-
-
ALDH-2
P05091
-
ALDH-E1
-
-
-
-
ALDH-E2
-
-
-
-
ALDH1
-
-
-
-
ALDH1
-
isozyme
ALDH1
-
-
ALDH1
Phanerochaete chrysosporium ATCC 34541
-
isoform
-
ALDH1-NL
-
-
-
-
ALDH1A1
-
-
ALDH1A1
-
isoform
ALDH1A1
-
isozyme
ALDH1A1
O35945
isozyme
Aldh1a2
-
previously known as retinaldehyde dehydrogenase 2
Aldh1a3
-
isozyme
Aldh1a7
O35945
isozyme
Aldh1b1
-
isoform
Aldh1b1
P30837
-
Aldh1b1
Q9CZS1
isoform, formerly known as ALDHx or ALDH5
Aldh1b1
Q9CZS1
isozyme
ALDH2
-
-
ALDH2
P05091
isoform
ALDH2
P47738
-
ALDH2
P47738
isoform
ALDH2
P47738
isozyme
ALDH2
Phanerochaete chrysosporium ATCC 34541
-
isoform
-
ALDH2
P11884
-
ALDH2
P11884
isoform
ALDH2(1)
-
-
ALDH2(2)
-
mutant enzyme
ALDH2(2)
-
-
ALDH2(3)
-
-
ALDH22A1
-
isoform
ALDH2C4
-
isoform
ALDH3A1
-
isoform
ALDH3A1
-
-
ALDH3A1
Q3UNF5
isoform
ALDH3A1
-
isoform
ALDH3A2
-
isozyme
ALDH3A2
P47740
isozyme
Aldh3b1
-
isozyme
ALDH3H1
-
cytosolic isoform
ALDH5A
P51650
-
ALDH7
Q9FPK6
-
Aldh7a1
-
-
Aldh7a1
-
isozyme
Aldh7a1
-
-
Aldh7a1
Q9DBF1
isoform
Aldh8a1
Q9H2A2
-
Aldh8a1
Q8BH00
isozyme
ALDHI
-
-
-
-
ALDHX
-
-
-
-
ALHDII
-
-
-
-
Allergen Alt a 10
-
-
-
-
aryl-aldehyde dehydrogenases
-
-
benzaldehyde dehydrogenase
-
-
Brassica turgor-responsive/drought-induced gene 26 protein
-
-
-
-
Btg-26
-
-
-
-
class 1 aldehyde dehydrogenase
-
-
class 2 aldehyde dehydrogenase
-
-
CoA-independent aldehyde dehydrogenase
-
-
-
-
coniferyl-aldehyde dehydrogenase
-
-
ETA-crystallin
-
-
-
-
gamma-aminobutyraldehyde dehydrogenase
-
-
-
-
hALDH2
-
-
irreversible NAD+-dependent aldehyde dehydrogenase
-
-
irreversible NAD+-dependent aldehyde dehydrogenase
-
-
-
K(+)-ACDH
-
-
-
-
K(+)-activated acetaldehyde dehydrogenase
-
-
-
-
m-methylbenzaldehyde dehydrogenase
-
-
-
-
Matured fruit 60 kDa protein
-
-
-
-
MF-60
-
-
-
-
Mg(2+)-ACDH
-
-
-
-
Mg(2+)-activated acetaldehyde dehydrogenase
-
-
-
-
mitochondrial aldehyde dehydrogenase
-
-
mitochondrial aldehyde dehydrogenase
P47738
-
NAD+-dependent aldehyde dehydrogenase
A6T782
-
NAD+-dependent aldehyde dehydrogenase
Klebsiella pneumoniae DSM 2026
A6T782
-
-
NAD+-linked aldehyde dehydrogenase
-
-
-
-
NAD+-pimelic semialdehyde-dependent aldehyde dehydrogenase
-
-
NAD+-pimelic semialdehyde-dependent aldehyde dehydrogenase
Sphingopyxis macrogoltabida TFA
-
-
-
NAD+-specific ALDH
A6T782
-
NAD+-specific ALDH
Klebsiella pneumoniae DSM 2026
A6T782
-
-
NAD-aldehyde dehydrogenase
-
-
-
-
NAD-aldehyde dehydrogenase
-
-
NAD-dependent 4-hydroxynonenal dehydrogenase
-
-
-
-
NAD-dependent aldehyde dehydrogenase
-
-
-
-
NAD-linked aldehyde dehydrogenase
-
-
-
-
Non-lens ALDH1
-
-
-
-
P51
-
-
-
-
PhnN
Q402C7
-
PhnN
Sphingomonas sp. 14DN61
Q402C7
-
-
phosphonoacetaldehyde dehydrogenase
-
-
phosphonoacetaldehyde oxidase
Q92UV7
-
PM-ALDH9
-
-
-
-
PnAA dehydrogenase
-
-
propionaldehyde dehydrogenase
-
-
-
-
PuuC
Klebsiella pneumoniae DSM 2026
A6T782
-
-
R-aminobutyraldehyde dehydrogenase
-
-
-
-
RALDH
-
-
-
-
RALDH(II)
-
-
-
-
RalDH1
-
-
-
-
RalDH1
-
-
Retinal dehydrogenase
-
-
-
-
retinal dehydrogenase type I
-
-
sALDH
-
-
salvery aldehyde dehydrogenase
-
-
ThnG
Sphingopyxis macrogoltabida TFA
-
-
-
Turgor-responsive protein 26G
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9028-86-8
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain K-12
-
-
Manually annotated by BRENDA team
isoenzyme E1, E2, and E3
-
-
Manually annotated by BRENDA team
isoenzyme E3
-
-
Manually annotated by BRENDA team
usual livers contains cytosolic ALDH1 and ALDH2, the atypical phenotype contains only the ALDH1 isoenzyme and immunologically cross-reactive material resulting from a mutation of the ALDH2 locus. 50% of orientals are atypical in respect to ALDH isoenzymes
-
-
Manually annotated by BRENDA team
Klebsiella pneumoniae DSM 2026
-
UniProt
Manually annotated by BRENDA team
isoform ALDH1B1
UniProt
Manually annotated by BRENDA team
isoform ALDH2
SwissProt
Manually annotated by BRENDA team
isoform ALDH3A1
UniProt
Manually annotated by BRENDA team
isoform ALDH7A1
UniProt
Manually annotated by BRENDA team
Nocardia sp. 239
239
-
-
Manually annotated by BRENDA team
cultivar Dongjin
UniProt
Manually annotated by BRENDA team
Japanese medaka
-
-
Manually annotated by BRENDA team
Phanerochaete chrysosporium ATCC 34541
-
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa IH 35342
IH 35342
-
-
Manually annotated by BRENDA team
strain ATCC 12633
-
-
Manually annotated by BRENDA team
enzyme from the promotion phase of hepatocarcinogenesis and phenobabital-inducible enzyme
-
-
Manually annotated by BRENDA team
enzyme I
-
-
Manually annotated by BRENDA team
membrane-bound ALDH, ALDH-I: soluble high Km ALDH, and ALDH-II: low Km ALDH
-
-
Manually annotated by BRENDA team
strain UPV-1
SwissProt
Manually annotated by BRENDA team
Rhodococcus erythropolis UPV-1
strain UPV-1
SwissProt
Manually annotated by BRENDA team
strain 14DN61
SwissProt
Manually annotated by BRENDA team
Sphingomonas sp. 14DN61
strain 14DN61
SwissProt
Manually annotated by BRENDA team
Sphingopyxis macrogoltabida TFA
-
-
-
Manually annotated by BRENDA team
Sulfolobus tokodaii 7
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
ALDH2 inhibition suppresses cocaine self-administration and seeking behavior and prevents cocaine- or cue-induced reinstatement in a rat model of cocaine relapse-like behavior
malfunction
-
ALDH7A1 knockdown sensitizes human renal proximal tubular epithelial cells and CHO cells to hyperosmotic stress
malfunction
-
decreased ALDH3A1 activity is associated with pathologic corneas (cataract development)
malfunction
P47738, Q3UNF5, Q9CZS1, Q9DBF1
decreased ALDH3A1 activity is associated with pathologic corneas (cataract development)
malfunction
Q9FPK6
endosperms from aldh7 null mutants accumulate brown pigments (melanoidin) during desiccation and storage
malfunction
-
inhibition of ALDH2 leads to accumulation of cytotoxic 4-hydroxynonenal and malondialdehyde, which can cause cell death through activation of c-Jun N-terminal protein kinase and/or p38 kinase
malfunction
-
the loss of ALDH2 enzyme activity leads to increased mitochondrial oxidative stress in aortic endothelia by three pro-oxidant stimuli, nitroglycerin, doxorubicin, and acetaldehyde
malfunction
-
vascular tolerance to nitroglycerin may be caused by impaired nitroglycerin bioactivation due to inactivation of mitochondrial aldehyde dehydrogenase ALDH2
metabolism
-
isoform ALDH3A1 may exhibit antioxidant activity indirectly by producing NADH during metabolism
metabolism
P47738, Q3UNF5, Q9CZS1, Q9DBF1
isoform ALDH3A1 may exhibit antioxidant activity indirectly by producing NADH during metabolism
physiological function
-
ALDH-2 plays a major role in nitroglycerin biotransformation
physiological function
-
ALDH2 plays an essential role in nitroglycerin bioactivation. ALDH2-catalyzed superoxide formation may essentially contribute to oxidative stress in nitroglycerin-exposed blood vessels
physiological function
-
ALDH2 reduces cardiac damage caused by ischaemia insult. ALDH2 protects the heart from aldehyde toxicity and is able to elicit signalling events that can protect against myocardial damages caused by acute ethanol toxicity
physiological function
-
ALDH7A1 is involved in cellular defense against hyperosmotic stress
physiological function
P47738, Q3UNF5, Q9CZS1, Q9DBF1
isoform ALDH1A1 plays a protective role against ultraviolet radiation-induced oxidative damage to ocular tissues
physiological function
P47738, Q3UNF5, Q9CZS1, Q9DBF1
isoform ALDH3A1 plays a protective role against ultraviolet radiation-induced oxidative damage to ocular tissues. The nuclear presence of isoform ALDH3A1 may be involved in cell cycle regulation
physiological function
-
isoforms ALDH3A1 plays a protective role against ultraviolet radiation-induced oxidative damage to ocular tissues. The nuclear presence of isoform ALDH3A1 may be involved in cell cycle regulation
physiological function
Q9FPK6
rice ALDH7 is needed for seed maturation and viability. ALDH7 is involved in removing various aldehydes formed by oxidative stress during seed desiccation
physiological function
-
the proliferation rate and the ability to form colonies is significantly increased by the overexpression of isoform ALDH2
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E)-4-hydroxy-2-nonenal + NAD+ + H2O
(2E)-4-hydroxy-2-nonenoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
(R)-trans-4-hydroxy-2-nonenal + NAD+ + H2O
(2E,4R)-4-hydroxynon-2-enoic acid + NADH + H+
show the reaction diagram
P51650
ALDH5A enantioselectively oxidizes (R)-trans-4-hydroxy-2-nonenal
-
-
?
(S)-trans-4-hydroxy-2-nonenal + NAD+ + H2O
(2E,4S)-4-hydroxynon-2-enoic acid + NADH + H+
show the reaction diagram
P51650
-
-
-
?
1,3-dihydroxyacetone + NAD+ + H2O
? + NADH + H+
show the reaction diagram
-
53.93% activity compared to acetaldehyde
-
-
?
1-formyl-6-methylpyrene + NAD+
6-methylpyrene 1-carboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
1-formyl-6-methylpyrene + NAD+ + H2O
6-methylpyrene-1-carboxylic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
1-formyl-8-methylpyrene + NAD+ + H2O
8-methylpyrene-1-carboxylic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
1-formyl-8-methylpyrene + NADP+
8-methylpyrene 1-carboxylate + NADPH + H+
show the reaction diagram
-
-
-
-
?
1-formylpyrene + NAD+
pyrene 1-carboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
1-formylpyrene + NAD+ + H2O
pyrene-1-carboxylic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
1-naphthaldehyde + NAD+ + H2O
1-naphthoic acid + NADH
show the reaction diagram
Q402C7
46.9% activity compared to p-tolualdehyde
-
-
?
1-naphthaldehyde + NAD+ + H2O
1-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
2,4-dinitrobenzaldehyde + NAD+ + H2O
2,4-dinitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
2-chlorobenzaldehyde + NAD+ + H2O
2-chlorobenzoate + NADH + H+
show the reaction diagram
-
28.35% activity compared to formaldehyde
-
-
?
2-formylpyrene + NAD+
pyrene 2-carboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-formylpyrene + NAD+ + H2O
pyrene-2-carboxylic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
2-furaldehyde + NAD+ + H2O
2-furanoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
2-furaldehyde + NAD+ + H2O
2-furanoic acid + NADH + H+
show the reaction diagram
A6T782
the activity on 2-furaldehyde is 22% of that on 3-hydroxypropionaldehyde
-
-
?
2-hydroxy-3-nitrobenzaldehyde + NAD+ + H2O
2-hydroxy-3-nitrobenzoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
2-naphthoate + NADH
show the reaction diagram
-
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
2-naphthoic acid + NADH
show the reaction diagram
Q402C7
48.8% activity compared to p-tolualdehyde
-
-
?
2-naphthaldehyde + NAD+ + H2O
2-naphthalene carboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-naphthaldehyde + NAD+ + H2O
2-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
2-nonenal + NAD+ + H2O
(E)-non-2-enoate + NADH + H+
show the reaction diagram
-
-
-
-
?
2-phthalaldehyde + NAD+ + H2O
phthalate + NADH + H+
show the reaction diagram
-
10.81% activity compared to formaldehyde
-
-
?
2-quinolinone-4-carboxaldehyde + NAD+ + H2O
2-quinolinone-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
3,4,5-trimethoxybenzaldehyde + NAD+ + H2O
3,4,5-trimethoxybenzoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
3,4-dihydroxybenzoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxymandelic aldehyde + NAD+ + H2O
3,4-dihydroxymandelate + NADH
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenyl acetaldehyde + NAD+ + H2O
3,4-dihydroxyphenylacetate + NADH
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenylacetaldehyde + NAD+ + H2O
3,4-dihydroxyphenylacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenylacetaldehyde + NAD+ + H2O
3,4-dihydroxyphenylacetate + NADH + H+
show the reaction diagram
P30837, Q9H2A2
-
-
-
?
3,4-dihydroxyphenylacetaldehyde + NAD+ + H2O
3,4-dihydroxyphenylacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenylacetaldehyde + NAD+ + H2O
3,4-dihydroxyphenylacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
3,4-dihydroxyphenylglycolaldehyde + NAD+ + H2O
3,4-dihydroxyphenylglycolate + NADH + H+
show the reaction diagram
P30837, Q9H2A2
-
-
-
?
3,4-dimethoxy-5-hydroxybenzaldehyde + NAD+ + H2O
3,4-dimethoxy-5-hydroxybenzoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
3,4-dimethoxybenzaldehyde + NAD+ + H2O
3,4-dimethoxybenzoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
3,4-dimethyoxybenzaldehyde + NAD+ + H2O
3,4-dimethoxybenzoate + NADH
show the reaction diagram
-
-
-
-
?
3-hydroxy-4-methoxybenzaldehyde + NAD+ + H2O
3-hydroxy-4-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
3-hydroxypropaldehyde + NAD+ + H2O
3-hydroxypropionate + NADH + H+
show the reaction diagram
-
-
-
-
?
3-hydroxypropionaldehyde + NAD+ + H2O
3-hydroxypropionic acid + NADH + H+
show the reaction diagram
-
-
-
-
r
3-hydroxypropionaldehyde + NAD+ + H2O
3-hydroxypropionate + NADH + H+
show the reaction diagram
A6T782
the activity on 3-hydroxypropionaldehyde is about 70% of that on valeraldehyde
-
-
?
3-hydroxypropionic acid + NADH + H2O
3-hydroxypropionaldehyde + NAD+ + H2O
show the reaction diagram
-
-
-
-
r
3-methoxy-4-hydroxyphenylacetaldehyde + NAD+ + H2O
3-methoxy-4-hydroxyphenylacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
3-nitrobenzaldehyde + NAD+ + H2O
3-nitrobenzoate + NADH + H+
show the reaction diagram
-
32.98% activity compared to acetaldehyde
-
-
?
3-oxopropylphosphonate + NAD+ + H2O
?
show the reaction diagram
Q92UV7
-
-
-
?
3-pyridinecarboxaldehyde + NAD+ + H2O
3-pyridinecarboxylate + NADH
show the reaction diagram
-
-
-
-
?
4-aminobutyraldehyde + NAD+ + H2O
4-aminobutyrate + NADH
show the reaction diagram
-
-
-
-
?
4-carboxybenzaldehyde + NAD+ + H2O
4-carboxybenzoate + NADH
show the reaction diagram
-
-
-
-
?
4-chlorobenzaldehyde + NAD+ + H2O
4-chlorobenzoate + NADH
show the reaction diagram
-
-
-
-
?
4-cyanobenzaldehyde + NAD+ + H2O
4-cyanobenzoate + NADH
show the reaction diagram
-
-
-
-
?
4-dimethylamino-1-naphthaldehyde + NAD+ + H2O
4-dimethylamino-1-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
4-dimethylaminobenzaldehyde + NAD+ + H2O
4-dimethylaminobenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-formylpyrene + NAD+
pyrene 4-carboxylate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-formylpyrene + NAD+ + H2O
pyrene-4-carboxylic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxy-2-nonenal + NAD+ + H2O
4-hydroxy-2-nonenoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxy-2-nonenal + NAD+ + H2O
4-hydroxy-2-nonenoate + NADH + H+
show the reaction diagram
-
low affinity for substrate binding and poor catalytic efficiency
-
-
?
4-hydroxy-2-nonenal + NAD+ + H2O
4-hydroxynon-2-enoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxy-2-nonenal + NAD+ + H2O
4-hydroxynon-2-enoic acid + NADH
show the reaction diagram
P47738, Q3UNF5, Q9CZS1, Q9DBF1
-
-
-
?
4-hydroxy-2-nonenal + NAD+ + H2O
4-hydroxynon-2-enoic acid + NADH
show the reaction diagram
P47738, Q3UNF5, Q9CZS1, Q9DBF1
poor substrate of isoform ALDH3A1
-
-
?
4-hydroxy-3,5-dimethoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3,5-dimethoxybenzoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
4-hydroxy-3-methoxybenzoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxybenzaldehyde + NAD+ + H2O
4-hydroxybenzoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxynon-2-enal + NAD+
4-hydroxynon-2-enoic acid + NADH
show the reaction diagram
-
-
-
-
?
4-hydroxynonenal + NAD+ + H2O
4-hydroxynonanoate + NADH
show the reaction diagram
-
-
-
-
?
4-hydroxynonenal + NAD+ + H2O
4-hydroxynonaoate + NADH
show the reaction diagram
-
-
-
-
?
4-hydroxynonenal + NAD+ + H2O
? + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxyphenyl-3-methoxyglycolaldehyde + NAD+ + H2O
4-hydroxyphenyl-3-methoxyglycolate + NADH
show the reaction diagram
-
-
-
-
?
4-hydroxyphenylglycolaldehyde + NAD+ + H2O
4-hydroxyphenylglycolate + NADH
show the reaction diagram
-
-
-
-
?
4-methoxy-1-naphthaldehyde + NAD+ + H2O
4-methoxy-1-naphthoate + NADH
show the reaction diagram
-
-
-
-
?
4-methoxy-1-naphthaldehyde + NAD+ + H2O
4-methoxy-1-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
4-methoxybenzaldehyde + NAD+ + H2O
4-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-nitro-1-naphthaldehyde + NAD+ + H2O
4-nitro-1-naphthoate + NADH
show the reaction diagram
-
-
-
-
?
4-oxonon-2-enal + NAD+ + H2O
4-oxonon-2-enoic acid + NADH
show the reaction diagram
-
-
-
-
?
5-bromo-1-naphthaldehyde + NAD+ + H2O
5-bromo-1-naphthoate + NADH
show the reaction diagram
-
-
-
-
?
5-hydroxyindol acetaldehyde + NAD+ + H2O
5-hydroxyindol acetate + NADH
show the reaction diagram
-
-
-
-
?
5-methoxyindole-3-carboxaldehyde + NAD+ + H2O
5-methoxyindole-3-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
6,7-dimethoxycoumarin-4-carboxaldehyde + NAD+ + H2O
6,7-dimethoxycoumarin-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
6-(dimethylamino)-2-naphthaldehyde + NAD+ + H2O
6-(dimethylamino)-2-naphthoate + NADH
show the reaction diagram
-
-
-
-
?
6-dimethylamino-2-naphthaldehyde + NAD+ + H2O
6-dimethylamino-2-naphthalene carboxylate + NADH + H+
show the reaction diagram
-
activity assay
-
-
?
6-dimethylamino-2-naphthaldehyde + NAD+ + H2O
6-dimethylamino-2-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
6-methoxy-2-naphthaldehyde + NAD+ + H2O
6-methoxy-2-naphthoate + NADH + H+
show the reaction diagram
-
-
-
-
?
6-methoxy-2-naphthaldehyde + NAD+ + H2O
6-methoxy-2-naphthoate + NADH + H+
show the reaction diagram
-
activity assay
-
-
?
6-methoxy-2-naphthaldehyde + NAD+ + H2O
6-methoxy-2-naphthalene carboxylate + NADH + H+
show the reaction diagram
-
activity assay
-
-
?
6-methoxy-2-naphthaldehyde + NAD+ + H2O
6-methoxy-2-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
6-methoxy-2-quinolinone-4-carboxaldehyde + NAD+ + H2O
6-methoxy-2-quinolinone-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
7-(dimethylamino)-2-quinolinone-4-carboxaldehyde + NAD+ + H2O
7-(dimethylamino)-2-quinolinone-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
7-(dimethylamino)coumarin-4-carboxaldehyde + NAD+ + H2O
7-(dimethylamino)coumarin-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
7-acetoxycoumarin-4-carboxaldehyde + NAD+ + H2O
7-acetoxycoumarin-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
7-hydroxycoumarin-4-carboxaldehyde + NAD+ + H2O
7-hydroxycoumarin-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
7-methoxy-1-naphthaldehyde + NAD+ + H2O
7-methoxy-1-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
7-methoxy-1-naphthaldehyde + NAD+ + H2O
7-methoxy-1-naphthoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
7-methoxy-2-quinolinone-4-carboxaldehyde + NAD+ + H2O
7-methoxy-2-quinolinone-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
7-methoxycoumarin-4-carboxaldehyde + NAD+ + H2O
7-methoxycoumarin-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
9-cis retinal + NAD+ + H2O
9-cis retinoic acid + NADH
show the reaction diagram
-
-
-
-
?
9-cis-retinal + NAD+ + H2O
9-cis-retinoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
-
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
P05091
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
P30837, Q9H2A2
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
ir
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
ir
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
ir
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
P51650
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
O35945, P47738, P47740, Q8BH00, Q9CZS1
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
Q9FPK6
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
preferred substrate
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
activity with cytosolic enzyme, no activity with microsomal enzyme
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
mutations in Aldh2 strongly segregate with the phenotypes of low and high alcohol consumption and with kinetic differences in ALDH2, suggesting that pharmacogenetic differences affect voluntary alcohol consumption. Bases mutated in the coding region suggest that aldh2(2) in low drinkers is ancestral to the coding changes of either aldh2(1) or aldh2(3), which segregate with higher ethanol consumption
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
activity assay
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
activity assay
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
P11884
rats with an mutated allele for aldehyde dehydrogenase (ALDH2-2) show a low alcohol consumption phenotype. Homozygous ALDH2-2 rats derived from high-drinker F0 females showed a remarkly higher ethanol consumption than homozygous animals derived from low-drinker females. Mitochondria of F2 rats derived from high alcohol-consuming females were more active in oxidizing substrates that generate NADH for complex I
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
P30837, Q9H2A2
preferred substrate of isozyme ALDH2
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
77.68% activity compared to formaldehyde
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
A6T782
the activity on acetaldehyde is about 50% of that on valeraldehyde
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
100% activity with acetaldehyde and NAD+
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
Nocardia sp. 239
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
Sulfolobus tokodaii 7
-
100% activity with acetaldehyde and NAD+
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
Pseudomonas aeruginosa IH 35342
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
Klebsiella pneumoniae DSM 2026
A6T782
the activity on acetaldehyde is about 50% of that on valeraldehyde
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
77.68% activity compared to formaldehyde
-
-
?
acetaldehyde + NADP+ + H2O
acetate + NADPH + H+
show the reaction diagram
-
low activity with NADP+
-
-
?
acrolein + NAD+ + H2O
? + NADH + H+
show the reaction diagram
-
-
-
-
?
aldophosphamide + NAD+ + H2O
? + NADH + H+
show the reaction diagram
-
-
-
-
?
all-trans retinal + NAD+ + H2O
all-trans retinoic acid + NADH
show the reaction diagram
-
-
-
-
?
all-trans-retinal + NAD+ + H2O
all-trans-retinoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
alpha-aminoadipate semialdehyde + NAD+ + H2O
alpha-aminoadipic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
alpha-phenylpropanal + NAD+ + H2O
alpha-phenylpropionate + NADH
show the reaction diagram
-
-
-
-
?
an aldehyde + NAD+ + H2O
an acid + NADH + H+
show the reaction diagram
-
-
-
-
?
anisaldehyde + NAD+ + H2O
? + NADH
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD(P)+ + H2O
benzoate + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
P05091
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
promotion-associated enzyme, no activity with phenobarbital-inducible enzyme
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
Q402C7
73.9% activity compared to p-tolualdehyde
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
72.38% activity compared to formaldehyde
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
best substrate, isoform ALDH1 shows 2fold greater catalytic activity against benzaldehyde than against other substrates, while isoform ALDH2 shows no substrate preferences
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
low affinity for benzaldehyde
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
A6T782
the activity on benzaldehyde is 35% of that on 3-hydroxypropionaldehyde
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
35.5% activity compared to acetaldehyde
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
Nocardia sp. 239
-
-
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
Sulfolobus tokodaii 7
-
35.5% activity compared to acetaldehyde
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
Klebsiella pneumoniae DSM 2026
A6T782
the activity on benzaldehyde is 35% of that on 3-hydroxypropionaldehyde
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
Sphingomonas sp. 14DN61
Q402C7
73.9% activity compared to p-tolualdehyde
-
-
?
benzaldehyde + NAD+ + H2O
benzoate + NADH + H+
show the reaction diagram
-
72.38% activity compared to formaldehyde
-
-
?
benzaldehyde + NADP+
benzoate + NADPH
show the reaction diagram
-
-
-
-
?
benzaldehyde + NADP+ + H2O
benzoate + NADPH + H+
show the reaction diagram
-
-
-
-
?
betaine aldehyde + NAD+ + H2O
betaine + NADH
show the reaction diagram
-
-
-
-
?
betaine aldehyde + NAD+ + H2O
betaine + NADH + H+
show the reaction diagram
-
-
-
-
?
butanal + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
-
-
-
-
?
butanal + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
-
-
-
-
?
butanal + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
-
-
-
-
?
butanal + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
-
-
-
-
?
butanal + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
Q4F895
-
-
-
?
butanal + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
-
n-butanal
-
-
?
butanal + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
-
n-butanal
-
-
?
butanal + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
Rhodococcus erythropolis UPV-1
Q4F895
-
-
-
?
butyraldehyde + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
-
71.65% activity compared to formaldehyde
-
-
?
butyraldehyde + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
Sulfolobus tokodaii, Sulfolobus tokodaii 7
-
48.15% activity compared to acetaldehyde
-
-
?
butyraldehyde + NAD+ + H2O
butyrate + NADH + H+
show the reaction diagram
-
71.65% activity compared to formaldehyde
-
-
?
butyraldehyde + NAD+ + H2O
butanoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
butyraldehyde + NAD+ + H2O
butanoate + NADH + H+
show the reaction diagram
A6T782
the activity on butyraldehyde is about 95% of that on valeraldehyde
-
-
?
chloral + NAD+ + H2O
trichloroacetate + NADH + H+
show the reaction diagram
-
2.67% activity compared to acetaldehyde
-
-
?
chloroacetaldehyde + NAD+ + H2O
chloroacetate + NADH + H+
show the reaction diagram
-
-
-
-
-
chloroacetaldehyde + NAD+ + H2O
chloroacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
chloroacetaldehyde + NAD+ + H2O
chloroacetate + NADH + H+
show the reaction diagram
-
activity is twice as fast as with acetaldehyde, native enzyme and mutant enzyme E487K
-
-
?
cinnamaldehyde + NAD+ + H2O
cinnamate + NADH + H+
show the reaction diagram
Sphingomonas sp., Sphingomonas sp. 14DN61
Q402C7
15.5% activity compared to p-tolualdehyde
-
-
?
cinnamic aldehyde + NAD+ + H2O
cinnamic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
citral + NAD+ + H2O
citrate + NADH
show the reaction diagram
-
-
-
-
?
crotonaldehyde + NAD+ + H2O
crotonate + NADH
show the reaction diagram
-
-
-
-
?
cyclohexanaldehyde + NAD+ + H2O
cyclohexanoate + NADH
show the reaction diagram
-
-
-
-
?
D-glucose + NAD+ + H2O
D-glucuronic acid + NADH
show the reaction diagram
-
-
-
-
?
D-glyceraldehyde + NAD+ + H2O
D-glycerate + NADH + H+
show the reaction diagram
-
50.25% activity compared to acetaldehyde
-
-
?
dansyl-aminoacetaldehyde + ?
dansylglycine + ?
show the reaction diagram
-
the fluorescent substrate is used to monitor aldehyde dehydrogenase activity in fluorescence-activated cell sorting
-
-
?
decanal + NAD+ + H2O
decanoate + NADH
show the reaction diagram
-
-
-
-
?
decanal + NAD+ + H2O
decanoate + NADH
show the reaction diagram
-
-
-
-
?
decanal + NAD+ + H2O
decanoate + NADH
show the reaction diagram
-
-
-
-
?
decanal + NAD+ + H2O
decanoate + NADH
show the reaction diagram
-
-
-
-
?
decanal + NAD+ + H2O
decanoate + NADH
show the reaction diagram
-
-
-
-
?
decanal + NAD+ + H2O
decanoate + NADH
show the reaction diagram
-
n-decylaldehyde
-
-
?
decanal + NAD+ + H2O
decanoate + NADH
show the reaction diagram
-
n-decylaldehyde
-
-
?
decanal + NAD+ + H2O
decanaote + NADH
show the reaction diagram
P76217
-
-
-
-
decanaldehyde + NAD+ + H2O
decanoate + NADH + H+
show the reaction diagram
-
50.3% activity compared to formaldehyde
-
-
?
DL-glyceraldehyde + NAD+ + H2O
DL-glycerate + NADH + H+
show the reaction diagram
-
77.27% activity compared to acetaldehyde, low activity with NADP+
-
-
?
DL-glyceraldehyde 3-phosphate + NAD+ + H2O
DL-glycerate 3-phosphate + NADH + H+
show the reaction diagram
-
52.7% activity compared to acetaldehyde
-
-
?
dodecanal + NAD+ + H2O
dodecanoate + NADH
show the reaction diagram
-
n-dodecylaldehyde
-
-
?
dodecanal + NAD+ + H2O
dodecanoate + NADH + H+
show the reaction diagram
-
best substrate
-
-
?
dodecanaldehyde + NAD+ + H2O
dodecanoate + NADH + H+
show the reaction diagram
-
42.02% activity compared to formaldehyde
-
-
?
eicosanoic aldehyde + NAD+ + H2O
eicosanoate + NADH + H+
show the reaction diagram
-
8.79% activity compared to formaldehyde
-
-
?
fluorene-2-carboxaldehyde + NAD+ + H2O
fluorene-2-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD+
formate + NADH + H+
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
-
-
-
-
ir
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
Q4F895
-
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
-
no activity
-
-
-
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
-
activity with cytosolic enzyme, no activity with microsomal enzyme
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
-
activity with enzyme ALDH-2, no activity with enzyme ALDH-1
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
Nocardia sp. 239
-
-
-
-
ir
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
Rhodococcus erythropolis UPV-1
Q4F895
-
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH + H+
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH + H+
show the reaction diagram
-
100% activity
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH + H+
show the reaction diagram
-
7.77% activity compared to acetaldehyde
-
-
?
glutaraldehyde + NAD+ + H2O
glutarate + NADH
show the reaction diagram
-
-
-
-
?
glutathione-4-oxonon-2-enal + NAD+ + H2O
glutathione-4-oxonon-2-enoic acid + NADH
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NAD+ + H2O
glycerate + NADH
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NAD+ + H2O
glycerate + NADH
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NAD+ + H2O
glycerate + NADH
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NAD+ + H2O
glycerate + NADH
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NAD+ + H2O
glycerate + NADH + H+
show the reaction diagram
-
-
-
-
?
glyceraldehyde + NAD+ + H2O
glycerate + NADH + H+
show the reaction diagram
Q9FPK6
-
-
-
?
glyceraldehyde 3-phosphate + NAD+ + H2O
glycerate 3-phosphate + NADH
show the reaction diagram
-
-
-
-
?
glyceraldehyde-3-phosphate + NAD+ + H2O
?
show the reaction diagram
Q92UV7
-
-
-
?
glyceryl trinitrate + NADH + H+
1,2-glyceryl dinitrate + nitrite + NAD+
show the reaction diagram
-
-
-
-
?
glyceryl trinitrate + NADH + H+
1,3-glyceryl dinitrate + nitrite + NAD+
show the reaction diagram
-
-
-
-
?
glycolaldehyde + NAD+ + H2O
glycolate + NADH
show the reaction diagram
-
-
-
-
-
glycolaldehyde + NAD+ + H2O
glycolate + NADH
show the reaction diagram
-
-
-
-
?
glycolaldehyde + NAD+ + H2O
glycolate + NADH
show the reaction diagram
-
-
-
-
?
glycolaldehyde + NAD+ + H2O
glycolate + NADH
show the reaction diagram
-
-
-
-
?
glycolaldehyde + NAD+ + H2O
glycolate + NADH
show the reaction diagram
-
the aldehyde dehydrogenase is a halophilic enzyme with the highest activity at 1 M NaCl
-
-
?
glycolaldehyde + NAD+ + H2O
glycolate + NADH + H+
show the reaction diagram
-
75.43% activity compared to acetaldehyde
-
-
?
glyoxal + NAD+ + H2O
oxalate + NADH
show the reaction diagram
-
-
-
-
?
heptanal + NAD+ + H2O
heptanoate + NADH
show the reaction diagram
-
-
-
-
?
heptanal + NAD+ + H2O
heptanoate + NADH
show the reaction diagram
-
-
-
-
?
heptanal + NAD+ + H2O
heptanoate + NADH
show the reaction diagram
-
n-heptanal
-
-
?
hexadecaldehyde + NAD+ + H2O
hexadecanoate + NADH + H+
show the reaction diagram
-
15.77% activity compared to formaldehyde
-
-
?
hexaldehyde + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
-
76.21% activity compared to formaldehyde
-
-
?
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
-
-
-
-
-
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
Q4F895
-
-
-
?
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
-
high specificity
-
-
?
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
-
n-hexanal
-
-
?
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
-
n-hexanal
-
-
?
hexanal + NAD+ + H2O
hexanoate + NADH + H+
show the reaction diagram
Rhodococcus erythropolis UPV-1
Q4F895
-
-
-
?
hydrocinnamaldehyde + NAD+ + H2O
hydrocinnamate + NADH
show the reaction diagram
-
-
-
-
?
indole-3-acetaldehyde + NAD+ + H2O
indole-3-acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
indole-3-acetaldehyde + NAD+ + H2O
indole-3-acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
indole-3-aldehyde + NAD+ + H2O
indole-3-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
indole-3-aldehyde + NAD+ + H2O
indole-3-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
isobutanal + NAD+ + H2O
isobutyrate + NADH
show the reaction diagram
-
-
-
-
?
isovaleraldehyde + NAD+ + H2O
isovalerate + NADH + H+
show the reaction diagram
-
-
-
-
?
isovaleraldehyde + NAD+ + H2O
isovalerate + NADH + H+
show the reaction diagram
A6T782
the activity on isovaleraldehyde is about 80% of that on valeraldehyde
-
-
?
m-chlorobenzaldehyde + NAD+ + H2O
m-chlorobenzoic acid + NADH + H+
show the reaction diagram
Q402C7
44.7% activity compared to p-tolualdehyde
-
-
?
m-fluorobenzaldehyde + NAD+ + H2O
m-fluorobenzoic acid + NADH + H+
show the reaction diagram
Sphingomonas sp., Sphingomonas sp. 14DN61
Q402C7
52.6% activity compared to p-tolualdehyde
-
-
?
m-hydroxybenzaldehyde + NAD+ + H2O
m-hydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
m-hydroxybenzaldehyde + NAD+ + H2O
m-hydroxybenzoic acid + NADH + H+
show the reaction diagram
Q402C7
23.6% activity compared to p-tolualdehyde
-
-
?
m-methoxybenzaldehyde + NAD+ + H2O
m-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
m-methoxybenzaldehyde + NAD+ + H2O
m-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
m-methoxybenzaldehyde + NAD+ + H2O
m-methoxybenzoic acid + NADH + H+
show the reaction diagram
Q402C7
88.5% activity compared to p-tolualdehyde
-
-
?
m-methylbenzaldehyde + NAD+ + H2O
m-methylbenzoate + NADH
show the reaction diagram
-
-
-
-
?
m-nitrobenzaldehyde + NAD+ + H2O
m-nitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
m-nitrobenzaldehyde + NAD+ + H2O
m-nitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
m-nitrobenzaldehyde + NAD+ + H2O
m-nitrobenzoic acid + NADH
show the reaction diagram
Q402C7
46.8% activity compared to p-tolualdehyde
-
-
?
m-tolualdehyde + NAD+ + H2O
m-methylbenzoic acid + NADH
show the reaction diagram
Q402C7
70.8% activity compared to p-tolualdehyde
-
-
?
malondialdehyde + NAD+ + H2O
? + NADH
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD+ + H2O
? + NADH
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD+ + H2O
? + NADH
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD+ + H2O
?
show the reaction diagram
P47738, Q3UNF5, Q9CZS1, Q9DBF1
-
-
-
?
malondialdehyde + NAD+ + H2O
?
show the reaction diagram
Q9FPK6
-
-
-
?
malondialdehyde + NAD+ + H2O
?
show the reaction diagram
-
poor substrate of isoform ALDH3A1
-
-
?
malondialdehyde + NAD+ + H2O
?
show the reaction diagram
-
low affinity for substrate binding and poor catalytic efficiency
-
-
?
malondialdehyde + NAD+ + H2O
? + NADH + H+
show the reaction diagram
-
-
-
-
?
methylglyoxal + NAD+ + H2O
methylglyoxalate + NADH
show the reaction diagram
-
-
-
-
?
N-acetyl-4-aminobutyraldehyde + NAD+ + H2O
N-acetyl-4-aminobutyrate + NADH
show the reaction diagram
-
-
-
-
?
n-nonanal + NAD+ + H2O
n-nonanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
n-nonanal + NAD+ + H2O
n-nonanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
n-nonanal + NAD+ + H2O
n-nonanoate + NADH + H+
show the reaction diagram
-
high specificity
-
-
?
nitroglycerin + NAD+ + H2O
1,2-glyceryl dinitrate + NO + NADH + H+
show the reaction diagram
-
-
-
-
?
nonanal + NAD+ + H2O
nonanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
o-chlorobenzaldehyde + NAD+ + H2O
o-chlorobenzoic acid + NADH + H+
show the reaction diagram
Q402C7
3.4% activity compared to p-tolualdehyde
-
-
?
o-fluorobenzaldehyde + NAD+ + H2O
o-fluorobenzoic acid + NADH + H+
show the reaction diagram
Sphingomonas sp., Sphingomonas sp. 14DN61
Q402C7
81.6% activity compared to p-tolualdehyde
-
-
?
o-hydroxybenzaldehyde + NAD+ + H2O
o-hydroxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
o-hydroxybenzaldehyde + NAD+ + H2O
o-hydroxybenzoic acid + NADH + H+
show the reaction diagram
Q402C7
65.2% activity compared to p-tolualdehyde
-
-
?
o-methoxybenzaldehyde + NAD+ + H2O
o-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
o-methoxybenzaldehyde + NAD+ + H2O
o-methoxybenzoic acid + NADH + H+
show the reaction diagram
Q402C7
2.6% activity compared to p-tolualdehyde
-
-
?
o-methylbenzaldehyde + NAD+ + H2O
o-methylbenzoate + NADH
show the reaction diagram
-
-
-
-
?
o-nitrobenzaldehyde + NAD+ + H2O
o-nitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
o-nitrobenzaldehyde + NAD+ + H2O
o-nitrobenzoic acid + NADH
show the reaction diagram
Q402C7
17.8% activity compared to p-tolualdehyde
-
-
?
o-tolualdehyde + NAD+ + H2O
o-methylbenzoic acid + NADH
show the reaction diagram
Q402C7
3.7% activity compared to p-tolualdehyde
-
-
?
octadecanal + NAD+ + H2O
octadecanoate + NADH + H+
show the reaction diagram
-
12% activity compared to formaldehyde
-
-
?
octanal + NAD+ + H2O
octanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
octanal + NAD+ + H2O
octanoate + NADH + H+
show the reaction diagram
-
high specificity
-
-
?
octanal + NAD+ + H2O
octanoate + NADH
show the reaction diagram
-
-
-
-
?
octanal + NAD+ + H2O
octanoate + NADH
show the reaction diagram
-
-
-
-
?
octanal + NAD+ + H2O
octanoate + NADH
show the reaction diagram
-
-
-
-
?
octanal + NAD+ + H2O
octanoate + NADH
show the reaction diagram
Q4F895
-
-
-
?
octanal + NAD+ + H2O
octanoate + NADH
show the reaction diagram
-
n-octylaldehyde
-
-
?
octanal + NAD+ + H2O
octanoate + NADH
show the reaction diagram
Rhodococcus erythropolis UPV-1
Q4F895
-
-
-
?
octyl aldehyde + NAD+ + H2O
octanoate + NADH + H+
show the reaction diagram
-
72.27% activity compared to formaldehyde
-
-
?
p-(dimethylamino)-benzaldehyde + NAD+ + H2O
p-(dimethylamino)benzoate + NADH
show the reaction diagram
-
-
-
-
?
p-(dimethylamino)cinnamaldehyde + NAD+ + H2O
p-(dimethylamino)cinnamate + NADH
show the reaction diagram
-
-
-
-
?
p-(dimethylamino)cinnamaldehyde + NAD+ + H2O
p-(dimethylamino)cinnamate + NADH
show the reaction diagram
-
-
-
-
-
p-carboxybenzaldehyde + NAD+ + H2O
p-carboxybenzoate + NADH
show the reaction diagram
-
-
-
-
?
p-chlorobenzaldehyde + NAD+ + H2O
p-chlorobenzoic acid + NADH + H+
show the reaction diagram
Q402C7
79.6% activity compared to p-tolualdehyde
-
-
?
p-fluorobenzaldehyde + NAD+ + H2O
p-fluorobenzoic acid + NADH + H+
show the reaction diagram
Sphingomonas sp., Sphingomonas sp. 14DN61
Q402C7
65.5%activity compared to p-tolualdehyde
-
-
?
p-hydroxybenzaldehyde + NAD+ + H2O
p-hydroxybenzoic acid + NADH + H+
show the reaction diagram
Q402C7
4.2% activity compared to p-tolualdehyde
-
-
?
p-hydroxyphenylacetaldehyde + NAD+ + H2O
p-hydroxyphenylacetate + NADH
show the reaction diagram
-
-
-
-
?
p-hydroxyphenylacetaldehyde + NAD+ + H2O
p-hydroxyphenylacetate + NADH
show the reaction diagram
-
-
-
-
?
p-methoxybenzaldehyde + NAD+ + H2O
p-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
p-methoxybenzaldehyde + NAD+ + H2O
p-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
p-methoxybenzaldehyde + NAD+ + H2O
p-methoxybenzoate + NADH + H+
show the reaction diagram
-
-
-
-
?
p-methoxybenzaldehyde + NAD+ + H2O
p-methoxybenzoic acid + NADH + H+
show the reaction diagram
Q402C7
6.2% activity compared to p-tolualdehyde
-
-
?
p-methylbenzaldehyde + NAD+ + H2O
p-methylbenzoate + NADH
show the reaction diagram
-
-
-
-
?
p-nitrobenzaldehyde + NAD+ + H2O
p-nitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
p-nitrobenzaldehyde + NAD+ + H2O
p-nitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
p-nitrobenzaldehyde + NAD+ + H2O
p-nitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
p-nitrobenzaldehyde + NAD+ + H2O
p-nitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
p-nitrobenzaldehyde + NAD+ + H2O
p-nitrobenzoate + NADH
show the reaction diagram
-
-
-
-
?
p-nitrobenzaldehyde + NAD+ + H2O
p-nitrobenzoic acid + NADH
show the reaction diagram
Q402C7
69% activity compared to p-tolualdehyde
-
-
?
p-nitrocinnamaldehyde + NAD+ + H2O
p-nitrocinnamate + NADH
show the reaction diagram
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
-
esterase activity of the various isoenzymes is less than 7% of the activity with propanal and NAD+
-
-
?
p-tolualdehyde + NAD+ + H2O
p-methylbenzoic acid + NADH
show the reaction diagram
Q402C7
highest activity
-
-
?
pentanal + NAD+ + H2O
pentanoate + NADH
show the reaction diagram
-
-
-
-
?
pentanal + NAD+ + H2O
pentanoate + NADH
show the reaction diagram
-
-
-
-
?
pentanal + NAD+ + H2O
pentanoate + NADH
show the reaction diagram
-
n-pentanal
-
-
?
phenanthrene-9-carboxaldehyde + NAD+ + H2O
phenanthrene-9-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
phenylacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
phenylacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
phenylacetate + NADH + H+
show the reaction diagram
-
-
-
-
?
phenylacetaldehyde + NAD+ + H2O
phenylacetate + NADH + H+
show the reaction diagram
-
37.86% activity compared to formaldehyde
-
-
?
phenylacetaldehyde + NAD+ + H2O
phenylacetate + NADH + H+
show the reaction diagram
-
4.21% activity compared to acetaldehyde
-
-
?
phosphonoacetaldehyde + NAD+
?
show the reaction diagram
-
-
-
-
?
phosphonoacetaldehyde + NAD+ + H2O
phosphonoacetate + NADH + H+
show the reaction diagram
Q92UV7
-
-
-
?
pimelic semialdehyde + NAD+ + H2O
?
show the reaction diagram
Sphingopyxis macrogoltabida, Sphingopyxis macrogoltabida TFA
-
-
-
-
?
pivaldehyde + NAD+ + H2O
pivalate + NADH
show the reaction diagram
-
-
-
-
?
propanal + 1,N6-ethenoadenine dinucleotide + H2O
propionate + ?
show the reaction diagram
-
isoenzyme E3
-
-
?
propanal + 2-acetylpyridine-NAD+ + H2O
propionate + 2-acetylpyridine-NADH
show the reaction diagram
-
-
-
-
?
propanal + 3-acetylpyridine-adenine dinucleotide + H2O
propionate + ?
show the reaction diagram
-
isoenzyme E3
-
-
?
propanal + 3-thionicotinamide-adenine dinucleotide + H2O
propionate + ?
show the reaction diagram
-
isoenzyme E3
-
-
?
propanal + deamino-NAD+ + H2O
propionate + deamino-NADH
show the reaction diagram
-
-
-
-
?
propanal + N-guanine dinucleotide + H2O
propionate + ?
show the reaction diagram
-
isoenzyme E3
-
-
?
propanal + N-hypoxanthine dinucleotide + H2O
propionate + ?
show the reaction diagram
-
isoenzyme E3
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
-
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
P05091
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
-
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH
show the reaction diagram
Nocardia sp. 239
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
propanal + NAD+ + H2O
propanoate + NADH + H+
show the reaction diagram
Rhodococcus erythropolis, Rhodococcus erythropolis UPV-1
Q4F895
-
-
-
?
propanal + NAD+ + H2O
propanoic acid + NADH + H+
show the reaction diagram
-
activity assay
-
-
?
propanal + [5-(3-acetylpyridino)pentyl]diphosphoadenosine + H2O
propanoate + ?
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
A6T782
the activity on propionaldehyde is about 55% of that on valeraldehyde
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
Sulfolobus tokodaii, Sulfolobus tokodaii 7
-
56.22% activity compared to acetaldehyde
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH + H+
show the reaction diagram
Klebsiella pneumoniae DSM 2026
A6T782
the activity on propionaldehyde is about 55% of that on valeraldehyde
-
-
?
propionaldehyde + NAD+ + H2O
propionate + NADH
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propanoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
propionaldehyde + NAD+ + H2O
propanoate + NADH + H+
show the reaction diagram
-
-
-
-
?
protocatechaldehyde + NAD+ + H2O
protocatechuic acid + NADH
show the reaction diagram
Q402C7
4.5% activity compared to p-tolualdehyde
-
-
?
pyridine 3-aldehyde + NAD+ + H2O
pyridine-3-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
pyridine-3-aldehyde adenine dinucleotide + NAD+ + H2O
pyridine-3-carboxylate adenine dinucleotide + NADH
show the reaction diagram
-
-
-
-
?
pyruvic aldehyde + NAD+ + H2O
pyruvate + NADH
show the reaction diagram
-
-
-
-
?
quinoline-3-carboxaldehyde + NAD+ + H2O
quinoline-3-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
quinoline-4-carboxaldehyde + NAD+ + H2O
quinoline-4-carboxylate + NADH
show the reaction diagram
-
-
-
-
?
retinal + NAD+ + H2O
retinoic acid + NADH + H+
show the reaction diagram
P30837, Q9H2A2
-
-
-
?
retinol + NAD+ + H2O
retinoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
succinic semialdehyde + NAD+ + H2O
succinate + NADH
show the reaction diagram
-
-
-
-
?
succinic semialdehyde + NAD+ + H2O
succinate + NADH
show the reaction diagram
P76217
-
-
-
-
syringaldehyde + NAD+ + H2O
syringic acid + NADH
show the reaction diagram
Q402C7
65.1% activity compared to p-tolualdehyde
-
-
?
tetradecanal + NAD+ + H2O
tetradecanoate + NADH
show the reaction diagram
-
high activity
-
-
?
tetradecyl aldehyde + NAD+ + H2O
tetradecanoate + NADH + H+
show the reaction diagram
-
11.71% activity compared to formaldehyde
-
-
?
trans-2-hexenal + NAD+ + H2O
(E)-hex-2-enoate + NADH + H+
show the reaction diagram
-
-
-
-
?
trans-2-nonenal + NAD+ + H2O
(E)-2-non-2-enoate + NADH + H+
show the reaction diagram
-
-
-
-
?
trans-cinnamaldehyde + NAD+ + H2O
trans-cinnamate + NADH
show the reaction diagram
-
-
-
-
?
trans-cinnamaldehyde + NAD+ + H2O
trans-cinnamate + NADH
show the reaction diagram
-
-
-
-
?
valeraldehyde + NAD+ + H2O
valerate + NADH + H+
show the reaction diagram
-
-
-
-
?
valeraldehyde + NAD+ + H2O
valerate + NADH + H+
show the reaction diagram
Klebsiella pneumoniae, Klebsiella pneumoniae DSM 2026
A6T782
most preferred substrate, 100% activity
-
-
?
vanillin + NAD+ + H2O
vanillic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
vanillin + NAD+ + H2O
vanillic acid + NADH + H+
show the reaction diagram
Q402C7
62.3% activity compared to p-tolualdehyde
-
-
?
monochloroacetaldehyde + NAD+ + H2O
monochloroacetate + NADH
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
enzyme also posseses esterase activity
-
-
-
additional information
?
-
-
the enzyme also catalyzes esterase reaction with p-nitrophenylacetate + H2O
-
-
-
additional information
?
-
-
the enzyme also catalyzes esterase reaction with p-nitrophenylacetate + H2O, enzyme also catalyzes esterase reaction with p-nitrophenyl pivalate + H2O
-
-
-
additional information
?
-
-
constitutive enzyme
-
-
-
additional information
?
-
-
enzyme ALD2 and ALD3 are stress-induced and glucose-repressed
-
-
-
additional information
?
-
-
the microsomal membrane-bound enzyme is expected to catalyze the oxidation of such water-insoluble aldehydes produced on the surface of the microsomal membrane. The enzyme may also be involved in the conversion of fatty acids to alpha,omega-dicarboxylic acids
-
-
-
additional information
?
-
-
the enzyme may play a role in insecticide resistance
-
-
-
additional information
?
-
-
the enzyme may contribute to corneal cellular defense against oxidative damage by metabolizing toxic aldehydes produced during UV-induced lipid peroxidation
-
-
-
additional information
?
-
-
the majority of the vascular ALDH2 is present in the cytoplasm, suggesting that mitochondrial biotransformation of glyceryl trinitrate by ALDH2 plays a minor role in the overall vascular biotransformation
-
-
-
additional information
?
-
-
no activity with benzaldehyde
-
-
-
additional information
?
-
-
the enzyme is only marginally active with acetaldehyde, while longer-chain aliphatic aldehydes are poor substrates
-
-
-
additional information
?
-
-
no activity with formaldehyde
-
-
-
additional information
?
-
-
no activity towards 3-hydroxypropionic acid in the presence of NADPH and formaldehyde
-
-
-
additional information
?
-
-
Aldh3a1 metabolizes toxic aldehydes produced by light-induced lipid peroxidation
-
-
-
additional information
?
-
-
involved in detoxication of a variety of aldehydes of plant origin, as well as those formed during food processing, or applied as food additives
-
-
-
additional information
?
-
-
ALDH2 is a direct downstream substrate of epsilon protein kinase C
-
-
-
additional information
?
-
-
ALDH2 denitrates nitroglycerin to 1,2-glyceryl dinitrate and nitrite but also catalyzes reduction of nitroglycerin to NO. In addition to aldehyde oxidation, ALDH2 catalyzes ester hydrolysis. The esterase activity is stimulated by NAD+, but the cofactor is not essential for the reaction
-
-
-
additional information
?
-
-
no activity is detected when NADP+ is used as the oxidant instead of NAD+
-
-
-
additional information
?
-
-
no activity towards NADP+, tetracosaldehyde, 2-hydroxylbenzaldehyde, ethanol, hexadecanol, acetate, and hexadecanoic acid
-
-
-
additional information
?
-
-
no activity with NADP+ and unsaturated aldehydes like trans-2-hexenal, trans-2-nonenal, and trans-2 dodecenal
-
-
-
additional information
?
-
A6T782
no appreciable activity is observed on formaldehyde
-
-
-
additional information
?
-
-
the ALDH has no reductive activity on acetate or hexadecanoic acid
-
-
-
additional information
?
-
-
the enzyme shows esterase activity with 4-nitrophenyl acetate
-
-
-
additional information
?
-
-
no activity with aromatic aldehydes such as benzaldehyde, nitrobenzaldehyde, methoxybenzaldehyde, or phenylacetaldehyde
-
-
-
additional information
?
-
-
no activity with methanol, ethanol and isopropanol
-
-
-
additional information
?
-
-
no activity with NADP+. Saturated aldehydes are preferred over unsaturated aldehydes, irrespective of chain length
-
-
-
additional information
?
-
Sulfolobus tokodaii 7
-
no activity with methanol, ethanol and isopropanol
-
-
-
additional information
?
-
Klebsiella pneumoniae DSM 2026
A6T782
no appreciable activity is observed on formaldehyde
-
-
-
additional information
?
-
-
no activity towards NADP+, tetracosaldehyde, 2-hydroxylbenzaldehyde, ethanol, hexadecanol, acetate, and hexadecanoic acid, the ALDH has no reductive activity on acetate or hexadecanoic acid
-
-
-
additional information
?
-
Sphingopyxis macrogoltabida TFA
-
no activity is detected when NADP+ is used as the oxidant instead of NAD+
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-hydroxy-2-nonenal + NAD+ + H2O
4-hydroxynon-2-enoate + NADH + H+
show the reaction diagram
-
-
-
-
?
4-hydroxy-2-nonenal + NAD+ + H2O
4-hydroxynon-2-enoic acid + NADH
show the reaction diagram
P47738, Q3UNF5, Q9CZS1, Q9DBF1
-
-
-
?
4-hydroxy-2-nonenal + NAD+ + H2O
4-hydroxynon-2-enoic acid + NADH
show the reaction diagram
P47738, Q3UNF5, Q9CZS1, Q9DBF1
poor substrate of isoform ALDH3A1
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
-
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
-
mutations in Aldh2 strongly segregate with the phenotypes of low and high alcohol consumption and with kinetic differences in ALDH2, suggesting that pharmacogenetic differences affect voluntary alcohol consumption. Bases mutated in the coding region suggest that aldh2(2) in low drinkers is ancestral to the coding changes of either aldh2(1) or aldh2(3), which segregate with higher ethanol consumption
-
-
?
acetaldehyde + NAD+ + H2O
acetate + NADH + H+
show the reaction diagram
P11884
rats with an mutated allele for aldehyde dehydrogenase (ALDH2-2) show a low alcohol consumption phenotype. Homozygous ALDH2-2 rats derived from high-drinker F0 females showed a remarkly higher ethanol consumption than homozygous animals derived from low-drinker females. Mitochondria of F2 rats derived from high alcohol-consuming females were more active in oxidizing substrates that generate NADH for complex I
-
-
?
an aldehyde + NAD+ + H2O
an acid + NADH + H+
show the reaction diagram
-
-
-
-
?
formaldehyde + NAD+ + H2O
formate + NADH
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD+ + H2O
?
show the reaction diagram
P47738, Q3UNF5, Q9CZS1, Q9DBF1
-
-
-
?
phosphonoacetaldehyde + NAD+
?
show the reaction diagram
-
-
-
-
?
phosphonoacetaldehyde + NAD+ + H2O
phosphonoacetate + NADH + H+
show the reaction diagram
Q92UV7
-
-
-
?
retinol + NAD+ + H2O
retinoic acid + NADH + H+
show the reaction diagram
-
-
-
-
?
malondialdehyde + NAD+ + H2O
?
show the reaction diagram
-
poor substrate of isoform ALDH3A1
-
-
?
additional information
?
-
-
constitutive enzyme
-
-
-
additional information
?
-
-
enzyme ALD2 and ALD3 are stress-induced and glucose-repressed
-
-
-
additional information
?
-
-
the microsomal membrane-bound enzyme is expected to catalyze the oxidation of such water-insoluble aldehydes produced on the surface of the microsomal membrane. The enzyme may also be involved in the conversion of fatty acids to alpha,omega-dicarboxylic acids
-
-
-
additional information
?
-
-
the enzyme may play a role in insecticide resistance
-
-
-
additional information
?
-
-
the enzyme may contribute to corneal cellular defense against oxidative damage by metabolizing toxic aldehydes produced during UV-induced lipid peroxidation
-
-
-
additional information
?
-
-
the majority of the vascular ALDH2 is present in the cytoplasm, suggesting that mitochondrial biotransformation of glyceryl trinitrate by ALDH2 plays a minor role in the overall vascular biotransformation
-
-
-
additional information
?
-
-
Aldh3a1 metabolizes toxic aldehydes produced by light-induced lipid peroxidation
-
-
-
additional information
?
-
-
involved in detoxication of a variety of aldehydes of plant origin, as well as those formed during food processing, or applied as food additives
-
-
-
additional information
?
-
-
ALDH2 is a direct downstream substrate of epsilon protein kinase C
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
-
-
NAD+
-
activates esterase activity
NAD+
-
all isoenzymes prefer NAD+ over NADP+
NAD+
P76217
-
NAD+
Q402C7
-
NAD+
Q4F895
totally inactive with NADP+
NAD+
-
dependent, the enzyme exhibits greater affinity for NAD+ than NADP+
NAD+
-
;
NAD+
P30837, Q9H2A2
required; required; required; required
NAD+
-
prefers NAD+ over NADP+
NAD+
P51650
;
NAD+
A6T782
NAD+ is the preferred coenzyme over NADP+
NAD+
-
isoform ALDH1B1 has an exclusive preference for NAD+ as the cofactor, no catalytic activity occurs when NADP+ is used
NAD+
-
the enzyme can utilize both NAD+ and NADP+ as cofactors and shows a preference toward NAD+
NAD+
-
dependent on, no activity with NADP+
NADP+
-
all isoenzymes prefer NAD+ over NADP+
NADP+
-
dependent, the enzyme exhibits greater affinity for NAD+ than NADP+
NADP+
-
prefers NAD+ over NADP+, in case of 3-hydroxypropionaldehyde, the activity is decreased by 73% when NAD+ is replaced with NADP+
NADP+
-
-
NADP+
-
the enzyme can utilize both NAD+ and NADP+ as cofactors and shows a preference toward NAD+
NADPH
-
-
[5-(3-acetylpyridino)pentyl]diphosphoadenosine
-
-
-
additional information
-
no activity with NADP+
-
additional information
-
NADP+: promotion-associated enzyme shows 1% of the activity with NAD+, phenobarbital-associated enzyme shows no activity
-
additional information
-
no activity with NADP+
-
additional information
-
very low activity
-
additional information
-
no activity with NADP+
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ba2+
-
the activity is elevated to 2-3fold in the presence of 2 mM Ba2+
BaCl2
-
0.1 mM, slight activation of microsomal enzyme
Ca2+
-
the activity is elevated to 2-3fold in the presence of 2 mM Ca2+
Ca2+
-
150% activity at 1 mM
CaCl2
-
0.1 mM, slight activation of microsomal enzyme
CsCl
-
0.1 mM, slight activation of microsomal enzyme
Iron
-
enzyme contains 2 iron-sulfur centres
K+
-
slight activation
K+
-
K+-activated enzyme
K+
-
activation seems to be due to increase of ionic strength; slight activation
K+
-
118% activity at 1 mM
K+
-
130% activity at 20 mM
Li+
-
119% activity at 20 mM
Mg2+
-
activates
Mg2+
-
activation of isoenzyme I and II
Mg2+
-
no effect
Mg2+
-
0.5 mM, slight activation
Mg2+
Q4F895
23% activation at 1 mM
Mg2+
-
-
Mg2+
P51650
Mg2+ ions stimulate the enantioselective oxidation of (R)-trans-4-hydroxy-2-nonenal by ALDH2 while suppressing (S)-trans-4-hydroxy-2-nonenal oxidation
Mg2+
-
180% activity at 1 mM
Mn2+
-
140% activity at 1 mM
Na+
-
122% activity at 20 mM
NaCl
-
0.1 mM, slight activation of microsomal enzyme
NH4+
Q4F895
13% activation at 1 mM
NH4+
-
131% activity at 20 mM
NiCl2
-
0.1 mM, slight activation of microsomal enzyme
Sr2+
-
the activity is elevated to 2-3fold in the presence of 2 mM Sr2+
Molybdenum
-
enzyme contains a molybdopterin binding domain
additional information
-
enzyme does not contain significant amounts of metal ion
additional information
-
1 mM Na+ has no effect on the enzyme activity
additional information
-
the enzyme is not influenced by 20 mM Mg2, Cu2+, Ca2+, Ba2+, Ni2+, Co2+, and Sr2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-(4-chlorophenyl)-3-(1-piperidinyl)-1-propanone
-
-
-
1-butanol
-
-
1-ethoxycyclopropanol
-
-
1-propanol
-
-
1-propanol
Q4F895
7% inhibition at 1 mM
2,4-dinitrobenzaldehyde
-
substrate inhibition
2,6-Dichloro-4-nitrophenol
-
primarily inhibits isoform ALDH2 (non-competitive), but does not affect isoform ALDH3A1
2-naphthaldehyde
-
substrate inhibition
3-(1-azepanyl)-1-phenyl-1-propanone
-
-
-
3-(dimethylamino)-1-(3-fluoro-4-methoxyphenyl)-1-propanone
-
-
-
3-(dimethylamino)-1-(4-ethylphenyl)-1-propanone
-
-
-
3-hydroxyanthranilic acid
-
46% inhibition at 0.002 mM
3-hydroxykynurenine
-
55% inhibition at 0.002 mM
4-amino-4-methyl-pent-2-ynthioic acid S-methylester
-
i.e. ampal thiolester, inhibits ALDH1 activity in ALDH1-transfected L1210T cells resistant to hydroperoxycyclophosphamide
4-diethylaminobenzaldehyde
-
-
4-diethylaminobenzaldehyde
-
potent inhibitor
4-diethylaminobenzaldehyde
-
DEAB
4-dimethylamino-4-methyl-pent-2-ynthioic acid S-methylester
-
competitive, 0.4 mM, 30 min, 80% inhibition
4-hydroxy-2-nonenal
-
4-hydroxy-2-nonenal completely inactivates ALDH2 activity in vitro at 100 mM
4-hydroxynon-2-enal
-
10% inhibition at 0.05 mM
4-methyl-4-morpholin-4-yl-pent-2-ynthioic acid S-methylester
-
0.6 mM, 60 min, 15% inhibition
4-oxonon-2-enal
-
90% inhibition at 0.05 mM
4-oxonon-2-enoic acid
-
90% inhibition at 0.05 mM
4-tetramethylammonium-4-methyl-pent-2-ynthioic acid S-methylester
-
0.6 mM, 60 min, 15% inhibition
5,5'-dithio-bis-(2-nitrobenzoic acid)
-
-
5-hydroxytryptophan
-
23% inhibition at 0.1 mM
6-[O-(CH2)5-COOH]-2-naphthaldehyde
-
substrate inhibition
acetaldehyde
-
substrate inhibition
acetaldehyde
-
0.02 mM, substrate inhibition of isoenzyme I, no inhibition of isoenzyme II and III
acetaldehyde
-
acetaldehyde inhibition of the enzyme is initiated below concentrations of 0.05 mM in the presence of 0.5 mM NAD or less
acetate
-
-
acrolein
-
-
adenine
-
-
adenosine
-
-
adenosine 5'-monophosphate
-
competitive with NAD+ and noncompetitive with acetaldehyde
ADPribose
-
competitive
ADPribose
-
-
Ag+
-
complete inhibition at 1 mM
Ag+
A6T782
complete inhibition at 1 mM
Alda-1
-
0.01 mM Alda-1 inhibits the apparent formaldehyde oxidation activity of the wild type enzyme by about 90%
arsenite
-
47% residual activity at 1 mM arsenite at pH 9.5, 28% residual activity at 2 mM arsenite at pH 7.4
BaCl2
-
0.1 mM, 10% inhibition of cytosolic enzyme
benzaldehyde
-
substrate inhibition
benzaldehyde
-
substrate inhibition
Butanal
-
substrate inhibition
c-Jun N-terminal protein kinase
-
incubation with catalytically active JNK leads to significant inhibition of ALDH2 activity. CCl4 exposure activates JNK which translocates to mitochondria and phosphorylates ALDH2 contributing to inhibition of ALDH2 activity
-
Ca2+
-
0.4 mM, 50% inhibition
Caffeine
-
-
Chloral hydrate
-
competitive with aldehyde
Chloral hydrate
-
competitive inhibition of hydrolysis of p-nitrophenyl acetate, mixed inhibition with p-nitrophenyl pivalate
Chloral hydrate
-
-
Chloral hydrate
-
competitive with respect to acetaldehyde
Chloral hydrate
-
inhibition of isoenzyme I and II from mitochondria and isoenzyme II from microsomes, no inhibition of isoenzyme I from microsomes
Chloral hydrate
-
-
Chloral hydrate
-
-
Chloroacetaldehyde
-
86% residual activity at 1 mM chloroacetaldehyde at pH 9.5
Chlorpropamide
-
this inhibition may play a role in cataract formation in patients maintained on systemic corticosteroids and in tablet-dependent diabetics
Co2+
-
50.3% residual activity at 1 mM
Co2+
A6T782
89.01% residual activity at 1 mM
Co2+
-
complete inhibition at 1 mM
CsCl2
-
0.1 mM, 19% inhibition of cytosolic enzyme
Cu2+
-
0.1 mM CuSO4, 7% inhibition of microsomal enzyme, 81% inhibition of the cytosolic enzyme
Cu2+
-
0.1 mM, CuCl2
Cu2+
-
2.9% residual activity at 1 mM
Cu2+
A6T782
complete inhibition at 1 mM
Cu2+
-
complete inhibition at 1 mM
cyanamide
-
-
cyanamide
-
complete inhibition at 0.5 mM
cyanamide
-
-
cyclohexanal
-
substrate inhibition at elevated aldehyde concentration, increase of NAD+ concentration from 0.85 mM to 2.55 mM removes substrate inhibition
D-glyceraldehyde
-
substrate inhibition
daidzin
-
-
daidzin
-
-
deoxycorticosterone
-
this inhibition may play a role in cataract formation in patients maintained on systemic corticosteroids and in tablet-dependent diabetics
diethyl disulfide
-
-
diethylaminobenzaldehyde
-
0.025 mM diethylaminobenzaldehyde inhibits about 80% of the enzymatic activity
-
dimethyl ampal thiolester
-
78% inhibition of isozyme ALDH1 at 0.0075 mM
Disulfiram
-
non-competitive
Disulfiram
-
inhibition of isoenzyme III, isoenzyme I is extremely sensitive, isoenzyme II is totally insensitive
Disulfiram
-
no effect on microsomal enzyme, complete inhibition of the cytosolic enzyme
Disulfiram
-
-
Disulfiram
-
-
Disulfiram
-
strong inhibition of isoenzyme 1, no inhibition of isoenzyme 2
Disulfiram
-
-
Disulfiram
-
inhibits activity of mitochondrial isoenzyme I with NAD+ and activity of isoenzyme I and II from microsomes and mitochondria with NADP+
Disulfiram
-
-
Disulfiram
-
38% inhibition at 0.05 mM, 93% inhibition at 0.1 mM
Disulfiram
P30837, Q9H2A2
0.01 mM; 0.01 mM; 0.01 mM; 0.01 mM
Disulfiram
-
42% inhibition at 0.002 mM
Disulfiram
-
72% inhibition at 0.002 mM
Disulfiram
-
at higher concentrations of propionaldehyde (0.5 and 1 mM), ALDH1B1 activity is inhibited by disulfiram, with the extent of inhibition being about 40% at 0.01 mM disulfiram
Disulfiram
-
0.025 mM diethylaminobenzaldehyde inhibits about 20-30% of the enzymatic activity
EDTA
Q4F895
10-20% inhibition at 1 mM
EDTA
-
85.3% residual activity at 1 mM
ethanol
-
-
ethanol
-
chronic or binge ethanol-exposure significantly decreases ALDH1 activity via S-nitrosylation, activity is restored by addition of dithiothreitol, ALDH1 activity (using 0.015 mM propionaldehyde as a substrate) is significantly inhibited (by 61%) in chronically ethanol-fed rats
Fe2+
-
57.5% residual activity at 1 mM
Fe2+
A6T782
26.34% residual activity at 1 mM
Fe3+
-
complete inhibition at 1 mM
formaldehyde
-
substrate inhibition
glyceraldehyde
-
substrate inhibition
H2O2
-
-
harman
-
18% inhibition at 0.1 mM
Hexanal
-
substrate inhibition
Hg2+
-
complete inhibition at 1 mM
Hg2+
A6T782
complete inhibition at 1 mM
HgCl2
-
-
indol-3-ylpyruvic acid
-
30% inhibition at 0.002 mM
indol-3-ylpyruvic acid
-
55% inhibition at 0.002 mM
Indole-3-acetaldehyde
-
substrate inhibition at elevated aldehyde concentration, increase of NAD+ concentration from 0.85 mM to 2.55 mM removes substrate inhibition
iodacetamide
Q4F895
10-20% inhibition at 1 mM
iodoacetamide
-
-
iodoacetamide
-
0.1 mM
Iodosobenzoate
-
0.1 mM, 3 min, complete inhibition
Isobutanal
-
substrate inhibition
K+
-
95.3% residual activity at 1 mM
KCl
-
0.1 mM, 16% inhibition
KCl
-
200 mM, 55% inhibition
KCN
Q4F895
10-20% inhibition at 1 mM
Kynurenic acid
-
40% inhibition at 0.002 mM
kynurenine
-
24% inhibition at 0.1 mM
Li+
-
2.8% residual activity at 1 mM
Li+
A6T782
90.64% residual activity at 1 mM
m-methylbenzaldehyde
-
substrate inhibition
methanol
-
-
methanol
Q4F895
17% inhibition at 1 mM
methidathione
-
-
methyl ampal thiolester
-
32% inhibition of isozyme ALDH1 at 0.0075 mM
methylglyoxal
-
substrate inhibition
Metyrapone
-
-
Mg2+
-
isoenzyme III
Mg2+
-
0.1 mM MgCl2, 63% inhibition of cytosolic enzyme
Mg2+
-
0.4 mM, 50% inhibition
Mg2+
-
85% inhibition at 0.5 mM
Mg2+
-
70% inhibition at 0.2 mM; 85% inhibition at 0.5 mM
Mg2+
-
80.6% residual activity at 1 mM
Mg2+
P51650
Mg2+ ions suppresses (R)-trans-4-hydroxy-2-nonenal oxidation by ALDH5A to a greater extent than that of (S)-trans-4-hydroxy-2-nonenal
Mg2+
A6T782
93.64% residual activity at 1 mM
Mg2+
-
wild type enzyme activity is inhibited (64%) by Mg2+ at pH 7.4, whereas the GFP-tagged enzyme activity is not affected by Mg2+
Mn2+
-
84.8% residual activity at 1 mM
molinate
-
irreversible inhibition of ALDH2
N'-methylnicotinamide
-
-
Na+
-
83.6% residual activity at 1 mM
Na+
A6T782
89.17% residual activity at 1 mM
NAD+
-
0.1 mM, significant inhibition of esterase activity
NAD+
-
pronounced inhibition occurs at above 5 mM
NADH
-
competitive with respect to NAD+, non-competitive with respect to acetaldehyde
NADH
-
competitive with NAD+ at high and low concentrations of acetaldehyde, product inhibitor
NADH
-
product inhibition
NADH
-
while acetaldehyde alone (1.6 mM) as an inhibiting factor yields a relative rate around 38% of the maximum experimental rate, the result with 0.18 mM initial NADH is 13% of the maximum experimental rate and further declines to 6.5% at 0.4 mM NADH
NEM
-
0.1 mM
NH3
-
79.8% residual activity at 1 mM
NH3
A6T782
92.06% residual activity at 1 mM
Ni2+
Q4F895
52% inhibition at 1 mM
Ni2+
-
72.4% residual activity at 1 mM
Ni2+
A6T782
89.54% residual activity at 1 mM
Ni2+
-
complete inhibition at 1 mM
NiCl2
-
0.1 mM, 24% inhibition of cytosolic enzyme
nitroglycerin
-
intravenous nitroglycerin treatment inhibits ALDH-2 activity, activity can be restored using dithiothreitol
nitroglycerin
-
ALDH2 dehydrogenase activity is inhibited by about 50% by large doses of nitroglycerin
nitroglycerin
-
complete inhibition at 0.05 mM nitroglycerin which can be reversed by the addition of 0.4 mM dithiothreitol
nitroglycerin
-
exposure of ALDH2 to nitroglycerin results in mechanism-based oxidative inactivation of the enzyme that is partially reversed by dithiothreitol
nitroglycerin
-
more than half of the original activity retained in the presence of 5.5 mM and approximate 80% of the activity is lost by the addition of 11 mM nitroglycerin
norharman
-
23% inhibition at 0.1 mM
o-nitrobenzaldehyde
-
substrate inhibition
p-hydroxyacetophenone
-
-
p-hydroxymercuribenzoate
-
-
p-hydroxymercuribenzoate
-
-
p-hydroxymercuribenzoate
-
0.1 mM, 3 min, complete inhibition
p-hydroxymercuribenzoate
-
0.05 mM, potent inhibition of isoenzyme I from microsomes and mitochondria, isoenzyme II from microsomes and mitochondria is less sensitive
p-hydroxymercuribenzoate
Q4F895
complete inhibition at 0.1 mM
p-hydroxyphenylacetaldehyde
-
substrate inhibition at elevated aldehyde concentration, increase of NAD+ concentration from 0.85 mM to 2.55 mM removes substrate inhibition
p-methylbenzaldehyde
-
substrate inhibition
p-nitrobenzaldehyde
-
substrate inhibition
p-nitrobenzaldehyde
-
substrate inhibition at elevated aldehyde concentration, increase of NAD+ concentration from 0.85 mM to 2.55 mM removes substrate inhibition
p-nitrocinnamaldehyde
-
substrate inhibition
p-nitrophenyl pivalate
-
inhibits dehydrogenase activity with propanal and D-glyceraldehyde
PCMB
-
-
PCMB
-
-
PCMB
-
-
pentanal
-
substrate inhibition
pivaldehyde
-
substrate inhibition at elevated aldehyde concentration, increase of NAD+ concentration from 0.85 mM to 2.55 mM removes substrate inhibition
PMSF
-
-
progesterone
-
this inhibition may play a role in cataract formation in patients maintained on systemic corticosteroids and in tablet-dependent diabetics
progesterone
-
-
propanal
-
substrate inhibition
propanal
-
competitive inhibition of hydrolysis of p-nitrophenyl acetate, mixed inhibition with p-nitrophenyl pivalate
pyridine 3-aldehyde
-
substrate inhibition at elevated aldehyde concentration, increase of NAD+ concentration from 0.85 mM to 2.55 mM removes substrate inhibition
Semicarbazide hydrochloride
-
86.7% residual activity at 1 mM
Sodium arsenite
-
-
Sodium bisulfite
-
1.8% residual activity at 1 mM
Tetraethylthiuram disulfide
-
disulfiram
theophylline
-
-
thiabendazole
-
-
thio-NAD+
-
-
thio-NAD+
-
-
xanthurenic acid
-
56% inhibition at 0.1 mM
Zn2+
Q4F895
73% inhibition at 1 mM
Zn2+
-
7% residual activity at 1 mM
Zn2+
A6T782
35.91% residual activity at 1 mM
[5-[3-(bromoacetyl)-pyridino]pentyl]diphosphoadenosine
-
-
-
molinate sulfoxide
-
i.e. S-ethyl-hexahydro-1H-azepine-1-carbothioate sulfoxide, irreversible inhibition of ALDH2
-
additional information
-
at low coenzyme concentrations, below 0.005 mM, and high aldehyde concentrations substrate inhibition of oxidation rates is observed
-
additional information
-
substrate inhibition by aromatic aldehydes
-
additional information
-
Durio zibethinus Murray fruit extract
-
additional information
-
inclusion of protease inhibitors, 1 mM benzamidine and 1 mM phenylmethylsulfonyl fluoride, in preparation of homogenate supernatants slightly inhibit ALDH activity
-
additional information
-
at the lowest concentration of substrate tested (propionaldehyde 0.1 mM), ALDH1B1 is not affected by disulfiram
-
additional information
-
EDTA has no effect on ALDH activity
-
additional information
-
activities of isoforms ALDH2 and ALDH3A1 are unaffected by pentachlorophenol (up to a concentration of 1 mM)
-
additional information
-
not influenced by acetaldehyde
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
100 mM, full activation
2-mercaptoethanol
-
highest activity at 50 mM
2-mercaptoethanol
-
27% increased activity at 1 mM
2-mercaptoethanol
-
about 140% activity at 5 mM
acetaldehyde
-
substrate activation at elevated aldehyde concentrations
Alda-1
-
Alda-1 increases the affinity of ALDH2 for NAD+ which results in increased ALDH2 activity
Alda-1
-
i.e. N-(1,3-benzodioxol-5-ylmethyl)-2,6-dichlorobenzamide, 0.01 mM Alda-1 increases acetaldehyde oxidation by wild type ALDH2 and the Asian variant E487K approximately 1.5 and 6fold, respectively. Alda-1 stimulates established ALDH2 activities by improving NAD+ binding but does not improve the nitroglycerin binding affinity of the Asian variant E487K
ascorbate
-
required
beta-mercaptoethanol
A6T782
161.09% activity at 1 mM
Butanal
-
substrate activation at elevated aldehyde concentrations
deoxycorticosterone
-
activates
diethylstilbestrol
-
activates
diphosphate
-
activates steady-state rate of oxidation of propanal
Disulfiram
-
0.1 mM, slight activation
dithiothreitol
-
4 mM, full activation
dithiothreitol
-
10 mM, enhances activity
dithiothreitol
-
75% increased activity at 1 mM
dithiothreitol
A6T782
147.48% activity at 1 mM
dithiothreitol
-
about 150% activity at 5 mM
estrone
-
activates
NAD+
-
stimulates hydrolysis of p-nitrophenyl esters
NADH
-
stimulates hydrolysis of p-nitrophenyl esters
NH4+
-
slight activation
NH4+
-
activation seems to be due to increase of ionic strength; slight activation
Oct1
-
activates gene expression of the corneal crystallin Aldh3a1
-
p300
-
activates gene expression of the corneal crystallin Aldh3a1
-
Pax6
-
activates gene expression of the corneal crystallin Aldh3a1
-
propanal
-
substrate activation at elevated aldehyde concentrations
Semicarbazide hydrochloride
A6T782
118.98% activity at 1 mM
Triton X-100
-
2% (v/v) Triton X-100 enhances the enzyme activity by 270%
GSH
-
30% activation
additional information
-
at high NAD+ concentrations, above 0.5 mM, and high aldehyde concentrations, above 0.05 mM propanal or above 1 mM acetaldehyde substrate activation of oxidation rates is observed
-
additional information
-
ThnG activity is not affected by the addition of 0.5 mM coenzyme A or dithiothreitol
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.023
(3,4-dihydroxyphenyl)(hydroxy)acetaldehyde
-
membrane-bound isoenzyme ALDH
0.001
(R)-trans-4-hydroxy-2-nonenal
P51650
ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37C
0.0007
(S)-trans-4-hydroxy-2-nonenal
P51650
ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37C
0.0358
1,N6-ethenoadenine dinucleotide
-
isoenzyme E3
0.00015
1-formyl-6-methylpyrene
-
ALDH2, at pH 7.5 and 37C
0.0016
1-formyl-6-methylpyrene
-
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.000027
1-formyl-8-methylpyrene
-
ALDH2, at pH 7.5 and 37C
0.004
1-formyl-8-methylpyrene
-
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.0009
1-formylpyrene
-
ALDH2, at pH 7.5 and 37C
0.004
1-formylpyrene
-
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.0000032
2,4-dinitrobenzaldehyde
-
enzyme ALDH-2
0.022
2-acetylpyridine-NAD+
-
-
-
0.00038
2-formylpyrene
-
ALDH2, at pH 7.5 and 37C
0.011
2-formylpyrene
-
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.000008
2-naphthaldehyde
-
enzyme ALDH-2
0.0004
2-naphthaldehyde
-
recombinant ALDH3A1
0.00046
2-naphthaldehyde
-
salivary ALDH
0.00024
2-naphthalene carboxylate
-
apparent Km value, diphosphate buffer, pH 8.1, cofactor NADH
0.0053
2-nonenal
-
pH and temperature not specified in the publication
0.0005
3,4-dihydroxyphenyl acetaldehyde
-
isonezyme II
0.001
3,4-dihydroxyphenyl acetaldehyde
-
pH 7.1, isoenzyme 2
0.003
3,4-dihydroxyphenyl acetaldehyde
-
isoenzyme III
0.017
3,4-dihydroxyphenyl acetaldehyde
-
membrane-bound isoenzyme ALDH
0.0004
3,4-dihydroxyphenylacetaldehyde
P30837, Q9H2A2
isozyme ALDH1A1 from liver
0.001
3,4-dihydroxyphenylacetaldehyde
P30837, Q9H2A2
isozyme ALDH2 from liver
0.0042
3,4-dihydroxyphenylacetaldehyde
P30837, Q9H2A2
isozyme ALDH1A1 from brain
0.00033
3,4-dimethoxybenzaldehyde
-
enzyme ALDH-2
0.29
3-hydroxypropionaldehyde
-
with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
0.48
3-hydroxypropionaldehyde
A6T782
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45C
0.49
3-hydroxypropionaldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
0.12
3-hydroxypropionic acid
-
with NADH as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
0.00196
3-Pyridinecarboxaldehyde
-
enzyme ALDH-2
0.0001
4-(dimethylamino)cinnamaldehyde
-
native enzyme
0.0003
4-(dimethylamino)cinnamaldehyde
-
mutant enzyme S74A
0.005
4-aminobutyrate
-
enzyme from liver
0.007
4-aminobutyrate
-
enzyme from brain
0.008
4-aminobutyrate
-
minor component of enzyme E3, pH 7.4
0.014
4-aminobutyrate
-
major component of enzyme E3, pH 7.4
0.014
4-Cyanobenzaldehyde
-
-
0.0042
4-Dimethylaminobenzaldehyde
-
recombinant ALDH3A1
0.00004
4-formylpyrene
-
ALDH2, at pH 7.5 and 37C
0.00078
4-formylpyrene
-
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
3.383
4-hydroxy-2-nonenal
-
at 25C, pH not specified in the publication
0.155
4-Hydroxy-3-methoxybenzaldehyde
-
recombinant ALDH3A1
0.0021
4-hydroxynonenal
-
pH 8.0, 25C
0.0179
4-hydroxynonenal
-
pH 8.0, 25C
0.0403
4-hydroxynonenal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22C
0.000065
4-methoxy-1-naphthaldehyde
-
enzyme ALDH-2
0.019
4-Methoxybenzaldehyde
-
recombinant ALDH3A1
0.0002
4-methyoxy-1-naphthaldehyde
-
enzyme ALDH-1
0.0000063
5-(dimethylamino)-2-naphthaldehyde
-
enzyme ALDH-1
0.0000004
5-bromo-1-naphthaldehyde
-
enzyme ALDH-2
0.0000025
5-bromo-1-naphthaldehyde
-
enzyme ALDH-1
0.026
5-hydroxyindol acetaldehyde
-
membrane-bound isoenzyme ALDH
0.0189
5-methoxyindole-3-carboxaldehyde
-
enzyme ALDH-2
0.0000004
5-nitro-1-naphthaldehyde
-
enzyme ALDH-2
0.000011
5-nitro-1-naphthaldehyde
-
enzyme ALDH-1
0.00069
6,7-dimethoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.0000023
6-(dimethylamino)-2-naphthaldehyde
-
enzyme ALDH-2
0.0017
6-dimethylamino-2-naphthaldehyde
-
apparent Km value, pyrophosphate buffer, pH 8.1, cofactor NAD+
0.0072
6-dimethylamino-2-naphthaldehyde
-
salivary ALDH
0.0079
6-dimethylamino-2-naphthaldehyde
-
apparent Km value, phosphate buffer, pH 7.3, cofactor NAD+
0.0086
6-dimethylamino-2-naphthaldehyde
-
apparent Km value, phosphate buffer, pH 6.2, cofactor NAD+
0.0114
6-dimethylamino-2-naphthaldehyde
-
apparent Km value, phosphate buffer, pH 8.0, cofactor NAD+
0.02
6-dimethylamino-2-naphthaldehyde
-
recombinant ALDH3A1
0.00016
6-methoxy-2-naphthaldehyde
-
recombinant ALDH3A1
0.0002
6-methoxy-2-naphthaldehyde
-
salivary ALDH
0.0054
6-methoxy-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
0.00213
7-(dimethylamino)-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
0.000062
7-(dimethylamino)-coumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.00006
7-acetoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.15
7-hydroxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.05
7-methoxy-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
0.00028
7-methoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.00005
acetaldehyde
-
major component of enzyme E3
0.0001
acetaldehyde
-
isoenzyme 1
0.0002
acetaldehyde
-
isoenzyme F2
0.00031
acetaldehyde
-
pH 9.5
0.00033
acetaldehyde
-
-
0.00036
acetaldehyde
-
pH 7.5
0.0004
acetaldehyde
-
native enzyme
0.0004
acetaldehyde
P51650
ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37C
0.00048
acetaldehyde
-
ALDH2, at pH 7.5 and 37C
0.00065
acetaldehyde
-
pH 8.0, 25C, wild-type enzyme
0.0015
acetaldehyde
-
-
0.002 - 0.003
acetaldehyde
-
isoenzyme 2
0.002
acetaldehyde
-
pH 9.0, isoenzyme E2
0.0024
acetaldehyde
-
isoenzyme 2, pH 9.5
0.0027
acetaldehyde
-
mutant enzyme S74A
0.003
acetaldehyde
-
pH 7.4, isoenzyme E2
0.003
acetaldehyde
-
isoenzyme E2
0.003
acetaldehyde
-
isoenzyme 2, pH 7.0
0.0032
acetaldehyde
-
0.1 M sodium diphosphate pH 8.0, 1 mM NAD+, 50 mM 2-mercaptoethanol, 1 mM pyrazole, 30 min, 25C
0.0035
acetaldehyde
-
isoenzyme ALDH2 from normal liver
0.005
acetaldehyde
-
isoenzyme ALDH-II
0.0066
acetaldehyde
-
in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60C
0.007
acetaldehyde
-
-
0.00879
acetaldehyde
-
pH 8.0, 25C, heteromeric enzyme obtained by coexpression of ALDH2 and ALDH2(2) in Escherichia coli
0.009
acetaldehyde
-
pH 9.5
0.01
acetaldehyde
-
100 mM Na2HPO4, pH 7.4, 100 mM NaCl, 1 mM NAD+, 25C
0.0115
acetaldehyde
-
0.1 M sodium diphosphate pH 8.0, 1 mM NAD+, 50 mM 2-mercaptoethanol, 1 mM pyrazole, 30 min, 25C
0.022
acetaldehyde
-
isoenzyme ALDH1 from normal liver
0.02573
acetaldehyde
-
pH 8.0, 25C, mutant enzyme ALDH2(2)
0.03
acetaldehyde
-
isoenzyme E1
0.04
acetaldehyde
-
enzyme from brain
0.04
acetaldehyde
-
-
0.048
acetaldehyde
-
isoenzyme III
0.05
acetaldehyde
-
enzyme from liver
0.055
acetaldehyde
-
at 25C, pH not specified in the publication
0.07
acetaldehyde
-
isoenzyme F1
0.082
acetaldehyde
-
-
0.12
acetaldehyde
-
isoenzyme 1, pH 9.5
0.13
acetaldehyde
-
-
0.165
acetaldehyde
-
-
0.18
acetaldehyde
-
enzyme ALDH-1, pH 7.5
0.18
acetaldehyde
-
enzyme form ALDH-1, pH 9.5
0.2
acetaldehyde
-
enzyme ALDH-2, pH 9.5
0.2
acetaldehyde
-
-
0.205
acetaldehyde
-
-
0.22
acetaldehyde
-
isoenzyme ALDH-I
0.246
acetaldehyde
-
-
0.4
acetaldehyde
-
-
0.83
acetaldehyde
-
at substrate concentrations 0.8-20 mM
1
acetaldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
2.4
acetaldehyde
-
enzyme I from microsome
2.5
acetaldehyde
-
isoenzyme 1, pH 7.0
4
acetaldehyde
-
-
5.8
acetaldehyde
P51650
ALDH5A, in 40 mM sodium phosphate buffer (pH 7.4), at 37C
6.38
acetaldehyde
-
in 50 mM Tris/HCl, at pH 8.0 and 80C
10
acetaldehyde
-
membrane-bound isoenzyme ALDH
80
acetaldehyde
-
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.017
acetylaldehyde
-
at substrate concentrations 0-0.07 mM
0.009
acrolein
-
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25C
0.169
alpha-aminoadipate semialdehyde
-
pH and temperature not specified in the publication
0.00093
alpha-phenylpropanal
-
enzyme ALDH-2
0.000018
benzaldehyde
-
enzyme ALDH-2
0.00006
benzaldehyde
-
pH 9.5
0.0001
benzaldehyde
-
native enzyme
0.0002
benzaldehyde
-
mutant enzyme S74A
0.00065
benzaldehyde
-
-
0.006
benzaldehyde
-
-
0.0399
benzaldehyde
-
at 25C and pH 8.5, with NADP+ as the electron acceptor
0.05
benzaldehyde
-
at 25C, pH not specified in the publication
0.0634
benzaldehyde
-
at 25C and pH 8.5, with NAD+ as the electron acceptor
0.148
benzaldehyde
-
recombinant ALDH3A1
0.15
benzaldehyde
-
100 mM Na2HPO4, pH 7.4, 100 mM NaCl, 1 mM NAD+, 25C
0.16
benzaldehyde
-
salivary ALDH
0.23
benzaldehyde
-
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.33
benzaldehyde
-
-
0.5302
benzaldehyde
-
pH and temperature not specified in the publication
1.3
benzaldehyde
-
phenobarbital-inducible enzyme
1.6
benzaldehyde
-
enzyme I from mitochondria
3
benzaldehyde
-
enzyme II from microsomes
5.37
benzaldehyde
-
with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
5.9
benzaldehyde
-
enzyme I from microsomes
50
benzaldehyde
-
enzyme II from mitochondria
0.0411
Betaine aldehyde
-
pH and temperature not specified in the publication
0.09
Betaine aldehyde
-
minor component of enzyme E3, pH 7.4
0.14
Betaine aldehyde
-
enzyme from brain
0.26
Betaine aldehyde
-
enzyme from liver
0.26
Betaine aldehyde
-
major component of enzyme form E3, pH 7.4
0.0234
betaNAD+
-
isoenzyme E3
0.00019
Butanal
-
-
0.0007
Butanal
-
native enzyme
0.002
Butanal
-
mutant enzyme S74A
0.0038
Butanal
Q4F895
1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37C
0.007
Butanal
-
-
0.26
Butyraldehyde
A6T782
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45C
0.97
Butyraldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
0.00055
cinnamaldehyde
-
-
0.006
cinnamic aldehyde
-
recombinant ALDH3A1
0.032
crotonaldehyde
-
-
1.136
D-glyceraldehyde
-
-
0.326
deamino-NAD+
-
-
0.0000029
decanal
-
enzyme form ALDH-1, pH 9.5
0.000022
decanal
-
enzyme form ALDH-2, pH 9.5
0.0013
decanal
-
-
0.0071
decanal
-
-
91
decanal
P76217
+/- 20
8.51
DL-glyceraldehyde
-
in 50 mM Tris/HCl, at pH 8.0 and 80C
6.12
DL-glyceraldehyde 3-phosphate
-
in 50 mM Tris/HCl, at pH 8.0 and 80C
0.005
dodecanal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22C
0.02
dodecanal
-
microsomal enzyme
0.000054
fluorene-2-carboxaldehyde
-
enzyme ALDH-1
0.031
formaldehyde
-
-
0.178
formaldehyde
-
native enzyme
0.32
formaldehyde
-
enzyme ALDH-2
0.333
formaldehyde
Q4F895
1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37C
0.42
formaldehyde
-
ALDH2, at pH 7.5 and 37C
0.65
formaldehyde
-
-
0.7
formaldehyde
-
mutant enzyme S74A
0.0009
Glutaraldehyde
-
native enzyme
0.003
Glutaraldehyde
-
mutant enzyme S74A
0.0062
Glutaraldehyde
-
isoenzyme ALDH-II
0.05
Glutaraldehyde
-
isoenzyme ALDH-I
0.56
Glutaraldehyde
-
membrane-bound isoenzyme ALDH
0.183
glutathione-4-oxonon-2-enal
-
-
0.17
glyceraldehyde
-
D-glyceraldehyde, L-glyceraldehyde, DL-glyceraldehyde
0.205
glyceraldehyde
-
-
0.39
glyceraldehyde
-
-
0.595
glyceraldehyde
-
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25C
7.374
glyceraldehyde
-
pH and temperature not specified in the publication
0.372
glyceraldehyde 3-phosphate
-
-
0.046
glycoaldehyde
-
isoenzyme 2, pH 7.4
0.005
glycolaldehyde
-
isoenzyme I
0.021
glycolaldehyde
-
isoenzyme II
0.038
glycolaldehyde
-
native enzyme
0.045
glycolaldehyde
-
-
0.077
glycolaldehyde
-
mutant enzyme S74A
0.11
glycolaldehyde
-
isoenzyme III
0.18
glycolaldehyde
-
-
0.2
glycolaldehyde
-
enzyme from brain
0.24
glycolaldehyde
-
enzyme from liver
0.69
glycolaldehyde
-
-
0.043
Glyoxal
-
-
0.000018
Heptanal
-
enzyme form ALDH-1, pH 9.5
0.000027
Heptanal
-
enzyme form ALDH-2, pH 9.5
0.0056
Heptanal
-
-
0.035
Heptanal
-
microsomal enzyme
0.00003
Hexanal
-
enzyme form ALDH-2, pH 9.5
0.000041
Hexanal
-
enzyme form ALDH-1, pH 9.5
0.0004
Hexanal
-
at 25C, pH not specified in the publication
0.0008
Hexanal
-
native enzyme
0.0018
Hexanal
Q4F895
1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37C
0.0024
Hexanal
-
mutant enzyme S74A
0.0035
Hexanal
-
pH 8.0, 25C
0.004
Hexanal
-
-
0.0134
Hexanal
-
pH 8.0, 25C
0.0391
Hexanal
-
pH and temperature not specified in the publication
0.071
Hexanal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22C
0.0005
hydrocinnamaldehyde
-
enzyme ALDH-2
0.0007
indole 3-acetaldehyde
-
isoenzyme I
0.001
indole 3-acetaldehyde
-
isoenzyme III
0.00015
Indole-3-acetaldehyde
-
enzyme ALDH-2
0.00031
Indole-3-acetaldehyde
-
enzyme ALDH-1
0.002
Indole-3-acetaldehyde
-
-
0.01 - 0.02
Indole-3-aldehyde
-
enzyme ALDH-2
0.03
Isobutanal
-
-
0.036
Isobutanal
-
-
0.21
Isovaleraldehyde
A6T782
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45C
0.68
Isovaleraldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
0.00009
m-methoxybenzaldehyde
-
enzyme ALDH-2
0.000018
m-methylbenzaldehyde
-
enzyme ALDH-2
0.0043
malondialdehyde
-
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25C
0.014
malondialdehyde
-
pH 8.0, 25C
0.1144
malondialdehyde
-
pH 8.0, 25C
0.13
malondialdehyde
-
-
0.466
malondialdehyde
-
at 25C, pH not specified in the publication
0.0086
methylglyoxal
-
pH 7.4, isoenzyme E2
0.021
methylglyoxal
-
pH 9.0, isoenzyme E2
0.552
methylglyoxal
-
minor component of enzyme form E3, pH 7.4
0.586
methylglyoxal
-
major component of enzyme form E3, pH 7.4
0.958
methylglyoxal
-
minor component of enzyme form E3, pH 9.0
1.876
methylglyoxal
-
major component of enzyme form E3, pH 9.0
0.11
N-acetyl-4-aminobutyrate
-
enzyme from liver
0.944
N-guanine dinucleotide
-
isoenzyme E3
0.172
N-hypoxanthine dinucleotide
-
isoenzyme E3
0.0008
n-nonanal
-
at 25C, pH not specified in the publication
0.0285
n-nonanal
-
pH and temperature not specified in the publication
0.0003
NAD+
-
50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,0.14 mM propanal, 25C
0.0012
NAD+
-
apparent Km value
0.0017
NAD+
-
pH 7.5, 25C, mutant enzyme S82A
0.0019
NAD+
-
reaction with propanal
0.0023
NAD+
-
enzyme from promotion phase of hepatocarcinogenesis
0.0026
NAD+
-
reaction with butanal
0.003
NAD+
-
reaction with propanal
0.003
NAD+
-
isoenzyme F1
0.0035
NAD+
-
pH 7.5, 25C, mutant enzyme D80G
0.0036
NAD+
-
50 mM sodium phosphate buffer, pH 7.4, 0.25 mM Mg2+,0.14 mM propanal, 25C
0.0036
NAD+
-
at 25C, pH not specified in the publication
0.004
NAD+
-
-
0.004
NAD+
-
native enzyme, pH 7.4
0.004
NAD+
-
brain enzyme
0.004
NAD+
-
pH 7.5, 25C, mutant enzyme R84Q
0.005
NAD+
-
isoenzyme ALDH1
0.0062
NAD+
-
50 mM sodium phosphate buffer, pH 7.4, 0.14 mM propanal, 25C
0.0077
NAD+
-
phenobarbital-inducible enzyme
0.008
NAD+
-
isoenzyme 1, pH 9.5
0.008
NAD+
-
pH 7.5, 25C, mutant enzyme ALDH1-5AA
0.01
NAD+
-
native enzyme, reaction with propanal
0.011
NAD+
-
pH 7.5, 25C, wild-type enzyme
0.011
NAD+
-
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25C
0.0125
NAD+
-
cytoplasmic isoenzyme
0.014 - 0.015
NAD+
-
reaction with 4-aminobutyraldehyde
0.014
NAD+
-
enzyme from liver
0.0145
NAD+
-
50 mM sodium phosphate buffer, pH 7.4, 0.14 mM propanal, 25C
0.015
NAD+
-
-
0.016
NAD+
-
isoenzyme ALDH2
0.018
NAD+
Q92UV7
mutant enzyme R108A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.022
NAD+
-
reaction with propanal, enzyme II from mitochondria
0.022
NAD+
-
pH 7.5, 25C, mutant enzyme ALDH1-H3tail
0.03
NAD+
-
isoenzyme ALDH-II
0.03
NAD+
-
isoenzyme F2
0.035
NAD+
-
-
0.036
NAD+
-
reaction with acetaldehyde
0.036
NAD+
-
reaction with propanal, enzyme I from mitochondria
0.0367
NAD+
-
reaction with acetaldehyde, at pH 9.5
0.037
NAD+
-
native enzyme
0.038
NAD+
-
reaction with butanal
0.038
NAD+
-
isoenzyme ALDH-I
0.0383
NAD+
-
reaction with acetaldehyde, at pH 7.5
0.04
NAD+
-
isoenzyme E1
0.04
NAD+
-
isoenzyme 1, pH 7.0
0.04
NAD+
Q92UV7
mutant enzyme N158A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.041
NAD+
-
enzyme from phenotype Aldh2(1)/Aldh2(3); enzyme from phenotype Aldh2(3)/Aldh2(3)
0.041
NAD+
-
50 mM sodium phosphate buffer, pH 7.4, 0.014 mM propanal, 25C
0.043
NAD+
-
enzyme from phenotype Aldh2(1)/Aldh2(1)
0.044
NAD+
-
reaction with butanal
0.047
NAD+
-
-
0.047
NAD+
-
100 mM Na2HPO4, pH 7.4, 100 mM NaCl, 1 mM NAD+, 25C
0.05
NAD+
-
-
0.054
NAD+
Q92UV7
mutant enzyme R447A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.058
NAD+
Q92UV7
wild type enzyme, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.06
NAD+
-
mutant enzyme R264Q
0.07
NAD+
-
native enzyme, pH 7.4
0.07
NAD+
-
isoenzyme E2
0.07
NAD+
-
isoenzyme 2, pH 7.0 and pH 9.5
0.074
NAD+
-
native enzyme, pH 9.0
0.075
NAD+
Q92UV7
mutant enzyme R290A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.077
NAD+
-
mitochondrial isoenzyme
0.09
NAD+
-
mutant enzyme E487Q
0.09
NAD+
Q4F895
0,05 mM propanal, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37C
0.09
NAD+
A6T782
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45C
0.095
NAD+
-
reaction with propanal, enzyme II from microsomes
0.097
NAD+
-
reaction with propanal, enzyme I from microsomes
0.119
NAD+
-
wild type enzyme, with nonanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22C
0.12
NAD+
-
50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,0.014 mM propanal, 25C
0.132
NAD+
-
enzyme from phenotype Aldh2(2)/Aldh2(2)
0.136
NAD+
-
mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25C
0.2
NAD+
-
reaction with decanal
0.37
NAD+
Q92UV7
mutant enzyme E385A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.389
NAD+
-
in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60C
0.4
NAD+
-
pH 9.5, reaction with acetaldehyde
0.421
NAD+
-
wild type enzyme, with hexanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22C
0.496
NAD+
-
mutant enzyme I200V, with hexanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22C
0.62
NAD+
-
reaction with formaldehyde
0.7
NAD+
-
mutant enzyme E487K, pH 7.4
0.74
NAD+
-
mutant enzyme R274E
0.82
NAD+
-
with Dl-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80C
0.84
NAD+
-
with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80C
0.85
NAD+
-
mutant enzyme R475Q
1
NAD+
-
at pH 7.5 and 30C
1.1
NAD+
-
mutant enzyme E487K, pH 9.0
1.3
NAD+
-
mutant enzyme R475Q/R264Q and R475E
1.9
NAD+
-
mutant enzyme S74A, reaction with propanal
3.218
NAD+
-
mutant enzyme I200G, with hexanal as cosubstrate, in 100 mM sodium diphosphate, at pH 8.0 and 22C
7.4
NAD+
-
mutant enzyme E487K
16
NAD+
-
mutant enzyme R475E/R264E
0.27
NADP+
-
reaction with benzaldehyde, enzyme II from microsomes
0.44
NADP+
-
reaction with benzaldehyde, enzyme I from microsomes; reaction with benzaldehyde, enzyme II from mitochondria
0.91
NADP+
-
reaction with benzaldehyde, enzyme I from mitochondria
3.81
NADP+
-
with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80C
8.47
NADP+
-
with Dl-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80C
0.008
Nonanal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22C
0.0008
o-methoxybenzaldehyde
-
enzyme ALDH-2
1.3
o-methylbenzaldehyde
-
enzyme ALDH-2
0.0000063
o-nitrobenzaldehyde
-
enzyme ALDH-2
0.000012
octanal
-
enzyme form ALDH-1, pH 9.5
0.000028
octanal
-
enzyme form ALDH-2, pH 9.5
0.000085
octanal
Q4F895
1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37C
0.007
octanal
-
pH 9.5
0.0175
octanal
-
pH and temperature not specified in the publication
0.029
octanal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22C
0.00002
p-(dimethylamino)-benzaldehyde
-
enzyme ALDH-2
0.00006
p-(dimethylamino)benzaldehyde
-
enzyme ALDH-1
0.000005
p-(dimethylamino)cinnamaldehyde
-
enzyme ALDH-2
0.0009
p-(dimethylamino)cinnamaldehyde
-
enzyme ALDH-1
0.00142
p-(dimethylamino)cinnamaldehyde
-
enzyme ALDH-1
0.024
p-Carboxybenzaldehyde
-
-
0.000018
p-Methoxybenzaldehyde
-
enzyme ALDH-2
0.0001
p-Methoxybenzaldehyde
-
native enzyme
0.0004
p-Methoxybenzaldehyde
-
mutant enzyme S74A
0.000017
p-methylbenzaldehyde
-
enzyme ALDH-2
0.000007
p-nitrobenzaldehyde
-
enzyme ALDH-2
0.0001
p-nitrobenzaldehyde
-
native enzyme
0.0004
p-nitrobenzaldehyde
-
mutant enzyme S74A
0.0034
p-nitrobenzaldehyde
-
-
0.018
p-nitrobenzaldehyde
-
isoenzyme ALDH-II
0.056
p-nitrobenzaldehyde
-
membrane-bound isoenzyme ALDH
2
p-nitrobenzaldehyde
-
isoenzyme ALDH-I
0.0000007
p-nitrocinnamaldehyde
-
enzyme ALDH-2
0.0043
p-nitrophenyl acetate
-
-
0.000034
pentanal
-
enzyme form ALDH-2, pH 9.5
0.00016
pentanal
-
enzyme form ALDH-1, pH 9.5
0.0007
pentanal
-
-
0.006
pentanal
-
-
0.000004
phenanthrene-9-carboxaldehyde
-
enzyme ALDH-2
0.000029
phenylacetaldehyde
-
enzyme ALDH-2
0.00352
phenylacetaldehyde
-
-
0.0055
phenylacetaldehyde
-
enzyme ALDH-1
0.027
phenylacetaldehyde
-
-
0.0032
phosphonoacetaldehyde
Q92UV7
wild type enzyme, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.005
phosphonoacetaldehyde
Q92UV7
mutant enzyme R290A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.009
phosphonoacetaldehyde
Q92UV7
mutant enzyme R108A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.019
phosphonoacetaldehyde
Q92UV7
mutant enzyme E385A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.029
phosphonoacetaldehyde
Q92UV7
mutant enzyme N158A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.15
phosphonoacetaldehyde
Q92UV7
mutant enzyme R447A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.5
phosphonoacetaldehyde
-
at pH 7.5 and 30C
0.000008
propanal
-
major component of enzyme E3
0.00001
propanal
-
minor component of enzyme E3
0.00004
propanal
-
minor component of enzyme E3
0.00008
propanal
-
native enzyme, pH 7.4
0.000095
propanal
-
enzyme form ALDH-2, pH 9.5
0.0001
propanal
-
native enzyme, pH 9.0
0.00011
propanal
-
pH 7.5
0.0002
propanal
-
mutant enzyme E487Q, pH 7.4
0.0002
propanal
-
50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25C; 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25C
0.00035
propanal
-
pH 9.5
0.0006
propanal
-
mutant enzyme E487K, pH 7.4
0.0007
propanal
-
native enzyme, pH 7.4
0.0007
propanal
-
-
0.0007
propanal
-
enzyme form ALDH-1, pH 9.5
0.0007
propanal
-
native enzyme
0.0007
propanal
-
isoenzyme 2, pH 7.0
0.0008
propanal
-
native enzyme and mutant enzyme S74A
0.0009
propanal
-
mutant enzyme S74A
0.0012
propanal
-
isoenzyme 2, pH 9.5
0.0012
propanal
-
50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25C
0.0014
propanal
-
mutant enzyme E487K, pH 7.4
0.0017
propanal
-
mutant enzyme E487K, pH 9.0
0.0023
propanal
-
-
0.0024
propanal
-
0.1 M sodium diphosphate pH 8.0, 1 mM NAD+, 50 mM 2-mercaptoethanol, 1 mM pyrazole, 30 min, 25C
0.0033
propanal
-
isoenzyme ALDH-II
0.0034
propanal
-
50 mM sodium phosphate buffer, pH 7.4, 0.25 mM Mg2+,1 mM NAD+, 25C
0.004
propanal
-
isoenzyme III
0.0045
propanal
-
enzyme form ALDH-1, pH 9.5
0.0052
propanal
-
50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25C
0.0053
propanal
-
100 mM Na2HPO4, pH 7.4, 100 mM NaCl, 1 mM NAD+, 25C
0.0058
propanal
-
50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25C
0.006
propanal
-
-
0.0061
propanal
-
0.1 M sodium diphosphate pH 8.0, 1 mM NAD+, 50 mM 2-mercaptoethanol, 1 mM pyrazole, 30 min, 25C
0.007
propanal
-
pH 9.5
0.0091
propanal
Q4F895
1 mM NAD+, 0,05 mM substrate, 250 mM sodium bicarbonate-NaOH buffer pH 10.0, 37C
0.011
propanal
-
isoenzyme 2, pH 9.5
0.0218
propanal
-
-
0.023
propanal
-
-
0.031
propanal
-
-
0.064
propanal
-
-
0.15
propanal
-
enzyme I and II from mitochondria
0.294
propanal
-
microsomal enzyme
0.5
propanal
-
isoenzyme ALDH-I
0.55
propanal
-
-
0.6474
propanal
-
pH and temperature not specified in the publication
0.765
propanal
-
at substrate concentrations 0.8-20 mM
1
propanal
-
isoenzyme 1, pH 7.0
1.3
propanal
-
enzyme from promotion phase of hepatocarcinogenesis
1.4
propanal
-
phenobarbital-inducible enzyme
1.7
propanal
-
enzyme II from microsome
2
propanal
-
membrane-bound isoenzyme ALDH
0.0028
propionaldehyde
-
pH 7.5, 25C, mutant enzyme D80G
0.0044
propionaldehyde
-
pH 7.5, 25C, mutant enzyme S82A
0.008
propionaldehyde
-
pH 7.5, 25C, mutant enzyme ALDH1-5AA
0.012
propionaldehyde
-
pH 7.5, 25C, wild-type enzyme
0.012
propionaldehyde
-
wild type isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25C
0.014
propionaldehyde
-
at 25C, pH not specified in the publication
0.031
propionaldehyde
-
pH 7.5, 25C, mutant enzyme ALDH1-H3tail
0.0662
propionaldehyde
-
pH 8.0, 25C
0.07
propionaldehyde
-
pH 7.5, 25C, mutant enzyme R84Q
0.1372
propionaldehyde
-
pH 8.0, 25C
0.189
propionaldehyde
-
mutant S82A/D80G of isoform ALDH1A1, in 100 mM sodium diphosphate (pH 9.5), at 25C
0.51
propionaldehyde
-
in 100 mM sodium diphosphate, at pH 8.0 and 22C
1.21
propionaldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
12
propionaldehyde
-
in 0.1 M sodium diphosphate buffer (pH 8.5), at ambient temperature
0.013
Pyruvic aldehyde
-
-
0.00033
quinoline-3-carboxaldehyde
-
enzyme ALDH-2
0.0028
quinoline-4-carboxaldehyde
-
enzyme ALDH-2
0.69
Succinic semialdehyde
P76217
+/- 0.22
3.54
Succinic semialdehyde
-
-
0.18
trans-2-hexenal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22C
0.003
trans-2-nonenal
-
in 100 mM sodium diphosphate, at pH 8.0 and 22C
0.000035
trans-cinnamaldehyde
-
enzyme ALDH-2
0.0004
trans-cinnamaldehyde
-
enzyme ALDH-1
0.14
Valeraldehyde
A6T782
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45C
0.24
Valeraldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
0.043
Monochloroacetaldehyde
-
-
additional information
additional information
-
Km-values of the 475 and 264 arginine mutants of Oriental variant E847K
-
additional information
additional information
-
influence of pH-value and buffer on Km-value for hexanal
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.039
(R)-trans-4-hydroxy-2-nonenal
P51650
ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37C
0.04
(S)-trans-4-hydroxy-2-nonenal
P51650
ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37C
0.667
2,4-dinitrobenzaldehyde
-
enzyme ALDH-2
4
2-naphthaldehyde
-
enzyme ALDH-2
1.57
2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
1.42
3,4-dimethoxybenzaldehyde
-
enzyme ALDH-2
4.91
3-hydroxypropionaldehyde
-
with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
28.54
3-hydroxypropionaldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
0.26
3-hydroxypropionic acid
-
with NADH as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
27.7
3-Pyridinecarboxaldehyde
-
enzyme ALDH-2
4.42
4-methoxy-1-naphthaldehyde
-
enzyme ALDH-1
2.67
5-(dimethylamino)-2-naphthaldehyde
-
enzyme ALDH-1
0.158
5-bromo-1-naphthaldehyde
-
enzyme ALDH-1
0.25
5-bromo-1-naphthaldehyde
-
enzyme ALDH-2
0.55
5-nitro-1-naphthaldehyde
-
enzyme ALDH-1
0.833
5-nitro-1-naphthaldehyde
-
enzyme ALDH-2
0.983
6,7-dimethoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.617
6-(dimethylamino)-2-naphthaldehyde
-
enzyme ALDH-2
0.983
6-methoxy-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
2.17
7-(dimethylamino)-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
1.3
7-(dimethylamino)-coumarin-3-carboxaldehyde
-
enzyme ALDH-2
7.9
7-(dimethylamino)coumarin-4-carboxaldehyde
-
enzyme ALDH-2
1.08
7-acetoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
8.33
7-hydroxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
5.33
7-methoxy-2-quinolinone-4-carboxaldehyde
-
enzyme ALDH-2
3.17
7-methoxycoumarin-3-carboxaldehyde
-
enzyme ALDH-2
0.26
acetaldehyde
P51650
ALDH2, in 40 mM sodium phosphate buffer (pH 7.4), at 37C
1.14
acetaldehyde
-
in 50 mM Tris/HCl, at pH 8.0 and 80C
1.86
acetaldehyde
-
-
3.5
acetaldehyde
P51650
ALDH5A, in 40 mM sodium phosphate buffer (pH 7.4), at 37C
11.03
acetaldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
13.2
acetaldehyde
-
enzyme form ALDH-1, pH 9.5
19.7
acetaldehyde
-
enzyme form ALDH-2, pH 9.5
15.2
alpha-phenylpropanal
-
enzyme ALDH-2
0.0425
benzaldehyde
-
at 25C and pH 8.5, with NADP+ as the electron acceptor
0.137
benzaldehyde
-
at 25C and pH 8.5, with NAD+ as the electron acceptor
5.83
benzaldehyde
-
enzyme ALDH-2
17.37
benzaldehyde
-
with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
26.95
Butyraldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
3.83
decanal
-
enzyme form ALDH-1, pH 9.5
11.7
decanal
-
enzyme form ALDH-2, pH 9.5
0.92
DL-glyceraldehyde
-
in 50 mM Tris/HCl, at pH 8.0 and 80C
0.83
DL-glyceraldehyde 3-phosphate
-
in 50 mM Tris/HCl, at pH 8.0 and 80C
2.83
fluorene-2-carboxaldehyde
-
enzyme ALDH-2
6
fluorene-2-carboxaldehyde
-
enzyme ALDH-1
67.5
formaldehyde
-
enzyme ALDH-2
0.04
glutathione-4-oxonon-2-enal
-
50 mM sodium phosphate buffer pH 7.4, 1 mM NAD+, 37C
1.29
glyceraldehyde
-
-
0.28
glyceraldehyde 3-phosphate
-
-
1.2
glycolaldehyde
-
-
4.33
Heptanal
-
enzyme form ALDH-1, pH 9.5
22.7
Heptanal
-
enzyme form ALDH-2, pH 9.5
4.17
Hexanal
-
enzyme form ALDH-1, pH 9.5
28.5
Hexanal
-
enzyme form ALDH-2, pH 9.5
12.3
hydrocinnamaldehyde
-
enzyme ALDH-2
9.33
Indole-3-acetaldehyde
-
enzyme ALDH-2
11.3
Indole-3-acetaldehyde
-
enzyme ALDH-1
27.31
Isovaleraldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
1
m-hydroxybenzaldehyde
-
enzyme ALDH-2
5.83
m-methoxybenzaldehyde
-
enzyme ALDH-2
4.5
m-methylbenzaldehyde
-
enzyme ALDH-2
0.0667
NAD+
-
mutant enzyme R475E/R264E
0.2
NAD+
-
mutant enzyme R264E
0.3
NAD+
-
mutant enzyme R475E
0.6
NAD+
-
with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80C
2
NAD+
-
mutant enzyme R475Q
2.05
NAD+
-
with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80C
2.08
NAD+
-
mutant enzyme R264Q
3.5
NAD+
-
native enzyme
1.63
NADP+
-
with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80C
3.71
NADP+
-
with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80C
0.367
o-methoxybenzaldehyde
-
enzyme ALDH-2
2.75
o-methylbenzaldehyde
-
enzyme ALDH-2
1.23
o-nitrobenzaldehyde
-
enzyme ALDH-2
4.17
octanal
-
enzyme form ALDH-2, pH 9.5
15
octanal
-
enzyme form ALDH-2, pH 9.5
2.33
p-(dimethylamino)-benzaldehyde
-
enzyme ALDH-2
2.13
p-(dimethylamino)benzaldehyde
-
enzyme ALDH-1
1.5
p-(dimethylamino)cinnamaldehyde
-
enzyme ALDH-2
8.17
p-(dimethylamino)cinnamaldehyde
-
enzyme ALDH-1
2.5
p-Methoxybenzaldehyde
-
enzyme ALDH-2
3
p-methylbenzaldehyde
-
enzyme ALDH-2
0.23
p-nitrobenzaldehyde
-
-
7.17
p-nitrobenzaldehyde
-
enzyme ALDH-2
0.45
p-nitrocinnamaldehyde
-
enzyme ALDH-2
0.26
p-nitrophenyl acetate
-
-
8.17
pentanal
-
enzyme form ALDH-1, pH 9.5
22.8
pentanal
-
enzyme form ALDH-2, pH 9.5
0.3
phenanthrene-9-carboxaldehyde
-
enzyme ALDH-2
1.39
phenylacetaldehyde
-
-
30
phenylacetaldehyde
-
enzyme ALDH-2
50
phenylacetaldehyde
-
enzyme ALDH-1
0.01
phosphonoacetaldehyde
Q92UV7
mutant enzyme N158A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.051
phosphonoacetaldehyde
Q92UV7
mutant enzyme R108A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.076
phosphonoacetaldehyde
Q92UV7
mutant enzyme R447A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.12
phosphonoacetaldehyde
Q92UV7
mutant enzyme R290A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.19
phosphonoacetaldehyde
Q92UV7
mutant enzyme E385A, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
0.42
propanal
-
50 mM sodium phosphate buffer, pH 7.4, 0.25 mM Mg2+,1 mM NAD+, 25C
0.65
propanal
-
50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25C
1.33
propanal
-
50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25C
1.48
propanal
-
50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25C
1.85
propanal
-
-
3.16
propanal
-
50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25C
6.16
propanal
-
50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25C
19.7
propanal
-
enzyme form ALDH-2
24.35
propionaldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
11.2
quinoline-3-carboxaldehyde
-
enzyme ALDH-2
4.67
quinoline-4-carboxaldehyde
-
enzyme ALDH-2
0.76
Succinic semialdehyde
-
-
2.5
trans-cinnamaldehyde
-
enzyme ALDH-2
7.83
trans-cinnamaldehyde
-
enzyme ALDH-1
26.24
Valeraldehyde
-
with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37C
1.19
malondialdehyde
-
-
additional information
additional information
-
turnover numbers of the 475 and 264 arginine mutants of Oriental variant E847K
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
2.2
phosphonoacetaldehyde
Q92UV7
wild type enzyme, with NAD+ in 50 mM HEPES, pH 7.5 supplemented with 10 mM MgSO4 for 5 min at 30C
additional information
propanal
-
50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
41.44
3-hydroxypropionaldehyde
A6T782
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45C
3709
0.18
acetaldehyde
-
in 50 mM Tris/HCl, at pH 8.0 and 80C
90
0.49
acetaldehyde
-
in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60C
90
117.2
Butyraldehyde
A6T782
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45C
499
0.11
DL-glyceraldehyde
-
in 50 mM Tris/HCl, at pH 8.0 and 80C
487
0.14
DL-glyceraldehyde 3-phosphate
-
in 50 mM Tris/HCl, at pH 8.0 and 80C
3338
121.9
Isovaleraldehyde
A6T782
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45C
2200
0.006
NAD+
-
in 50 mM KH2PO4-K2HPO4 buffer (pH 8.0), at 60C
7
0.73
NAD+
-
with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80C
7
2.45
NAD+
-
with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80C
7
254.7
NAD+
A6T782
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45C
7
0.19
NADP+
-
with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80C
10
0.98
NADP+
-
with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80C
10
207.1
Valeraldehyde
A6T782
recombinant enzyme, in 50 mM potassium phosphate buffer, at pH 8.0 and 45C
1481
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0074
2,6-Dichloro-4-nitrophenol
-
isoform ALDH2, in 0.1 M sodium diphosphate buffer (pH 9.5), at 21-23C
2.55
acetaldehyde
-
at 22C and pH 7.8
5
acetaldehyde
-
-
0.32
ADPribose
-
native enzyme
6.8
ADPribose
-
mutant enzyme S74A
0.37
benzaldehyde
-
-
2
benzaldehyde
-
-
4.35
Butanal
-
-
3.5
Caffeine
-
apparent Ki value
0.17
Chloral hydrate
-
-
14.32
D-glyceraldehyde
-
-
0.28
dimethyl ampal thiolester
-
pH 8.5, 37C
0.0002
Disulfiram
-
isoenzyme 1
6
formaldehyde
-
-
0.17
Hexanal
-
-
100.1
Isobutanal
-
-
2
methylglyoxal
-
major component of enzyme E3, pH 9.0
12
methylglyoxal
-
minor component of enzyme E3, pH 9.0
0.162
NADH
-
at 22C and pH 7.8
0.54
pentanal
-
-
2
propanal
-
-
7.3
theophylline
-
apparent Ki value
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0068
1-(4-chlorophenyl)-3-(1-piperidinyl)-1-propanone
-
isoform ALDH1A1, pH and temperature not specified in the publication
-
0.0083
1-(4-chlorophenyl)-3-(1-piperidinyl)-1-propanone
-
isoform ALDH2, pH and temperature not specified in the publication
-
0.0022
3-(1-azepanyl)-1-phenyl-1-propanone
-
isoform ALDH1A1, pH and temperature not specified in the publication
-
0.0086
3-(1-azepanyl)-1-phenyl-1-propanone
-
isoform ALDH2, pH and temperature not specified in the publication
-
0.0025
3-(dimethylamino)-1-(3-fluoro-4-methoxyphenyl)-1-propanone
-
isoform ALDH1A1, pH and temperature not specified in the publication
-
0.0064
3-(dimethylamino)-1-(3-fluoro-4-methoxyphenyl)-1-propanone
-
isoform ALDH2, pH and temperature not specified in the publication
-
0.0054
3-(dimethylamino)-1-(4-ethylphenyl)-1-propanone
-
isoform ALDH2, pH and temperature not specified in the publication
-
0.0079
3-(dimethylamino)-1-(4-ethylphenyl)-1-propanone
-
isoform ALDH1A1, pH and temperature not specified in the publication
-
2.5
Caffeine
-
-
3.5
theophylline
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.000047
-
tested group, n = 32, squamous cell cancer, mean
0.000048
-
tested group, n = 59, healthy tissue, mean
0.000049
-
tested group, n = 59, esophageal cancer, mean
0.00005
-
non-drinkers, n = 24, healthy tissue, mean
0.000051
-
drinkers, n = 35, healthy tissue, mean; non-drinkers, n = 24, healthy tissue, median
0.000052
-
non-drinkers, n = 24, esophageal cancer, mean; tested group, n = 27, adenocarcinoma, mean; tested group, n = 59, healthy tissue, median
0.000053
-
tested group, n = 32, squamous cell cancer, median
0.000054
-
drinkers, n = 35, esophageal cancer, mean; drinkers, n = 35, healthy tissue, median; non-drinkers, n = 24, esophageal cancer, median
0.000055
-
tested group, n = 59, esophageal cancer, median
0.000058
-
drinkers, n = 35, esophageal cancer, median
0.000059
-
tested group, n = 27, adenocarcinoma, median
0.000119
-
total group, n = 44, healthy tissue, mean
0.00012
-
non-drinkers, n = 25, healthy tissue, mean
0.000123
-
drinkers, n = 19, healthy tissue, mean; non-drinkers, n = 25, healthy tissue, median
0.000125
-
total group, n = 44, healthy tissue, median
0.000128
-
drinkers, n = 19, healthy tissue, median
0.000179
-
metastatic tumor, mean
0.00018
-
conversion of glycerol trinitrate to 1,3-glycerol dinitrate
0.000185
-
primary tumor, mean
0.000186
-
metastatic tumor, median
0.000188
-
non-drinkers, n = 25, cancer tissue, mean
0.00019
-
non-drinkers, n = 25, cancer tissue, median; total group, n = 44, cancer tissue, mean
0.000192
-
primary tumor, median
0.000193
-
total group, n = 44, cancer tissue, median
0.000194
-
drinkers, n = 19, cancer tissue, mean
0.000198
-
drinkers, n = 19, cancer tissue, median
0.00066
-
conversion of glycerol trinitrate to 1,3-glycerol dinitrate
0.00088
-
-
0.003
-
conversion of glycerol trinitrate to 1,2-glycerol dinitrate
0.009
-
conversion of glycerol trinitrate to 1,2-glycerol dinitrate
0.0636
Q402C7
substrate: o-methoxybenzaldehyde
0.0825
Q402C7
substrate: o-chlorobenzaldehyde
0.09
Q402C7
substrate: o-tolualdehyde
0.1029
Q402C7
substrate: p-hydroxybenzaldehyde
0.11
Q402C7
substrate: protocatechaldehyde
0.137
-
-
0.1516
Q402C7
substrate: p-methoxybenzaldehyde
0.2
-
-
0.232
-
enzyme I
0.2341
-
substrate: propionaldehyde, pH 8.0, 25C
0.29
-
isoenzyme I
0.37
-
isoenzyme F1
0.375
-
recombinant enzyme, using propionaldehyde as the substrate, in 50 mM sodium phosphate buffer, pH 7.4, at 37C
0.38
-
-
0.3812
Q402C7
substrate: cinnamaldehyde
0.4366
Q402C7
substrate: o-nitrobenzaldehyde
0.445
-
isoenzyme III
0.462
-
-
0.5
-
isoenzyme ALDH1 from atypical liver
0.51
-
ALDH1 from usual human liver
0.5337
-
substrate: propionaldehyde, pH 8.0, 25C
0.54
-
isoenzyme E1
0.58
-
isoenzyme 1
0.5811
Q402C7
substrate: m-hydroxybenzaldehyde
0.69
P76217
+/- 0.02, succinic semialdehyde
0.79
-
ALDH2 from usual human liver
0.8
-
isoenzyme F2
1.01
-
isoenzyme E2
1.1
Q402C7
substrate: m-chlorobenzaldehyde
1.14
-
-
1.15
Q402C7
substrate: m-nitrobenzaldehyde
1.154
Q402C7
substrate: 1-naphthaldehyde
1.2
Q402C7
substrate: 2-naphthaldehyde
1.292
Q402C7
substrate: m-fluorobenzaldehyde
1.3
-
isoenzyme E2
1.339
-
-
1.42
-
crude extract, using 3-hydroxypropionaldehyde as a substrate, with NAD+ as a cofactor, at pH 8.0 and 37C
1.53
Q402C7
substrate: vanillin
1.599
Q402C7
substrate: syringaldehyde
1.601
Q402C7
substrate: o-hydroxybenzaldehyde
1.61
Q402C7
substrate: p-fluorobenzaldehyde
1.696
Q402C7
substrate: p-nitrobenzaldehyde
1.739
Q402C7
substrate: m-tolualdehyde
1.815
Q402C7
substrate: beznaldehyde
1.957
Q402C7
substrate: p-chlorobenzaldehyde
2.006
Q402C7
substrate: o-fluorobenzaldehyde
2.07
-
enzyme from the promotion phase of hepatocarcinogenesis
2.175
Q402C7
substrate: m-methoxybenzaldehyde
2.457
Q402C7
substrate: p-tolualdehyde
3
-
isoenzyme II
3.13
A6T782
crude extract, using 3-hydroxypropionaldehyde as substrate, in 50 mM, potassium phosphate buffer, at pH 8.0 and 45C
3.86
-
phenobarbital-inducible enzyme
5
P76217
+/- 0.5, decanal
8.75
-
microsomal enzyme
27.73
A6T782
after 8.86fold purification, using 3-hydroxypropionaldehyde as substrate, in 50 mM, potassium phosphate buffer, at pH 8.0 and 45C
38.1
-
after 26.8fold purification, using 3-hydroxypropionaldehyde as a substrate, with NAD+ as a cofactor, at pH 8.0 and 37C
127
-
soluble isoenzyme ALDH-II
150
-
soluble isoenzyme ALDH-I
311
-
membrane-bound isoenzyme ALDH
additional information
-
-
additional information
-
-
additional information
-
men, n = 34, control, age 56 - 70, median 2.953 mU/l, range 1.721 - 6.227 mU/l; men, n = 34, gastric cancer, age 58 - 72, median 3.002 mU/l, range 1.734 - 7.038 mU/l; total group, n = 55, control, age 53 - 70, median 2.918 mU/l, range 1.635 - 6.227 mU/l; total group, n = 55, gastric cancer, age 54 - 72, median 2.983 mU/l, range 1.624 - 7.038 mU/l; women, n = 21, control, age 53 - 67, median 2.906 mU/l, range 1.635 - 5.224 mU/l; women, n = 21, gastric cancer, age 54 - 70, median 2.968 mU/l, range 1.624 - 6.163 mU/l
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.4
-
reaction with gamma-aminobutyrate
7.4
-
incubation of ALDH with 14C-labeled nitroglycerin
7.5
-
activity assay
8
-
reaction with indole 3-acetaldehyde, isoenzyme III
8
-
potassium phosphate buffer
8
-
activity assay
8.5
-
reaction with acetaldehyde and indole 3-acetaldehyde, isoenzyme II
8.5
P76217
-
8.5
-
activity assay
8.6
-
isoenzyme E1
8.6
-
isoenzyme 1
8.8
-
Tris-HCl buffer
8.8
-
microsomal isoenzyme I and II and mitochondrial isoenzymes with NADP+
8.8
-
activity assay
9
-
reaction with acetaldehyde, isoenzyme III; reaction with indole 3-acetaldehyde, isoenzyme I
9
-
microsomal enzyme
9
-
above, isoenzyme 2
9
-
activity assay
9.2 - 9.5
-
-
9.5 - 10.5
-
cytosolic enzyme
9.5
-
reaction with propanal
9.5
-
isoenzyme E2
9.5
Q4F895
with n-propanal as substrate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 9
-
about 60% activity at pH 6.0, about 70% activity at pH 7.0, 100% activity pH 8.0, about 85% activity at pH 9.0
7 - 9
-
pH 7.0: about 66% of maximal activity, pH 9.0: about 85% of maximal activity, isoenzyme 1
7 - 9
A6T782
-
7.3 - 9.5
-
50% of maximal activity at pH 7.3 and at pH 9.5
7.5 - 10
-
pH 7.5: about 35% of maximal activity, pH 10.0: about 60% of maximal activity
7.5 - 10
-
pH 7.5: about 40% of maximal activity, pH 10.0: about 60% of maximal activity, reaction with acetaldehyde
7.6
-
-
8 - 9.5
-
pH 8.0: about 35% of maximal activity, above pH 9.0: optimum, isoenzyme 2
8.5 - 10.5
-
pH 8.5: about 40% of the activity of isoenzyme ALDH1 and 2, pH 10.5: about 50% of the activity of isoenzyme ALDH2, about 65% of the activity of isoenzyme ALDH1
9 - 10
-
pH 9: about 35% of maximal activity, pH 10: optimum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
activity assay
37
-
at pH 7
37
-
incubation of ALDH with 14C-labeled nitroglycerin
47.5
Q4F895
with n-propanal as substrate
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
70 - 90
-
about 50% activity at 70C, 100% activity at 80C, about 80% activity at 90C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.7
-
two-dimensional gel-electrophoresis
4.9
-
about
5.3
Q4F895
two-dimensional electrophoresis
5.9
-
isoform ALDH1, calculated from amino acid sequence
6.1
-
isoform ALDH2, calculated from amino acid sequence
6.3
-
isoelectric focusing
7.7
-
precursor protein, isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
aldehyde dehydrogenase activity correlates with the viability of cells taken from bone marrow grafts
Manually annotated by BRENDA team
-
highly expressed
Manually annotated by BRENDA team
-
highest ALDH7A1 expression
Manually annotated by BRENDA team
Rhodococcus erythropolis UPV-1, Sphingomonas sp. 14DN61
-
-
-
Manually annotated by BRENDA team
-
increased activity in colorectal cancer cells
Manually annotated by BRENDA team
-
expressed
Manually annotated by BRENDA team
-
highly expressed
Manually annotated by BRENDA team
-
cells with high aldehyde dehydrogenase activity and CD133 surface marker expression possess an enhanced repopulation function
Manually annotated by BRENDA team
-
tissue with highest content
Manually annotated by BRENDA team
-
highest ALDH7A1 expression, ALDH7A1 in the kidney localizes to endothelial and podocyte cell nuclei
Manually annotated by BRENDA team
-
tissue with highest content
Manually annotated by BRENDA team
-
tissue with highest content
Manually annotated by BRENDA team
-
expressed
Manually annotated by BRENDA team
O35945, P47740, Q8BH00, Q9CZS1
isozyme Aldh8a1 mRNA expression is highest in liver
Manually annotated by BRENDA team
-
increased activity in hepatic cancer cells
Manually annotated by BRENDA team
O35945, P47740, Q8BH00, Q9CZS1
isozyme Aldh2 mRNA expression is highest in liver
Manually annotated by BRENDA team
-
highest ALDH7A1 expression
Manually annotated by BRENDA team
-
ALDH1A1 is preferentially expressed in the CD133+ subpopulation
Manually annotated by BRENDA team
-
highest expression of ALDH3A1 late in development
Manually annotated by BRENDA team
O35945, P47740, Q8BH00, Q9CZS1
isozyme Aldh1a1 mRNA expression is highest in lung