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Literature summary for 1.2.1.3 extracted from

  • Zimmerling, J.; Tischler, D.; Großmann, C.; Schlömann, M.; Oelschlägel, M.
    Characterization of aldehyde dehydrogenases applying an enzyme assay with in situ formation of phenylacetaldehydes (2017), Appl. Biochem. Biotechnol., 182, 1095-1107.
    View publication on PubMed
Search for more literature for 1.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 phenylacetaldehyde at pH 7.7 and 25°C Escherichia coli
0.5
-
 NAD+ at pH 7.7 and 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 3 months, 52% loss of activity Escherichia coli
0°C, purified enzyme, 3 months, 46% loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chlorophenylacetaldehyde + NAD+ + H2O
-
 Escherichia coli 4-chlorophenylacetate + NADH + H+
-
 ?
4-fluorophenylacetaldehyde + NAD+ + H2O
-
 Escherichia coli 4-fluorophenylacetate + NADH + H+
-
 ?
additional information the enzyme shows 11% activity with NADP+ compared to NAD+. No activity with phenazine methosulfate Escherichia coli ?
-
 ?
phenylacetaldehyde + NAD+ + H2O best substrate Escherichia coli phenylacetate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
FeaB-K-12
-
 Escherichia coli
phenylacetaldehyde dehydrogenase
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-20 55 after preincubation of the protein at -20 to 45°C, residual activities of 82.7 to 100% are measured. After storage at 50°C, this enzyme shows an activity of about 63.1%, whereas significant enzyme inactivation is observed after incubation at 55°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+ most suitable cofactor Escherichia coli