Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.3 extracted from

  • Moretti, A.; Li, J.; Donini, S.; Sobol, R.W.; Rizzi, M.; Garavaglia, S.
    Crystal structure of human aldehyde dehydrogenase 1A3 complexed with NAD+ and retinoic acid (2016), Sci. Rep., 6, 35710.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
complexed with NAD+ and retinoic acid, hanging drop vapor diffusion method Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0093
-
 all-trans-retinal at pH 8.0 and 25°C Homo sapiens
2.4
-
 acetaldehyde at pH 8.0 and 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56108
-
-
 Homo sapiens
224000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
all-trans-retinal + NAD+ + H2O Homo sapiens
-
 all-trans-retinoic acid + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA chromatography and Sephacryl S200 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
glioma cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
 Homo sapiens acetate + NADH + H+
-
 ?
all-trans-retinal + NAD+ + H2O
-
 Homo sapiens all-trans-retinoic acid + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
homotetramer 2 * 56108, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
aldehyde dehydrogenase 1A3
-
 Homo sapiens
Aldh1a3
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens