Literature summary for 1.2.1.3 extracted from

Stiti, N.; Podgorska, K.; Bartels, D.
Aldehyde dehydrogenase enzyme ALDH3H1 from Arabidopsis thaliana: Identification of amino acid residues critical for cofactor specificity (2014), Biochim. Biophys. Acta, 1844, 681-693.

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
E149D	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana
E149D/I200G	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana
E149D/I200V	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana
E149N	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana
E149N/I200G	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana
E149N/I200V	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana
E149Q	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana
E149Q/I200G	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana
E149Q/I200V	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana
E149T	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana
E149T/I200G	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana
E149T/I200V	the mutant shows a good catalysis with NADP+ compared to the wild type enzyme	Arabidopsis thaliana
E149T/V178R/I200V	the mutant uses NADP+ with almost 7fold higher catalytic efficiency compared to NAD+	Arabidopsis thaliana
I200G	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana
I200V	the mutant shows reduced catalytic efficiency compared to the wild type enzyme	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.003	-	trans-2-nonenal	wild type enzyme, at pH 8.0 and 25°C	Arabidopsis thaliana
0.008	-	Nonanal	wild type enzyme, at pH 8.0 and 25°C	Arabidopsis thaliana
0.0322	-	trans-2-nonenal	mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C	Arabidopsis thaliana
0.065	-	Nonanal	mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C	Arabidopsis thaliana
0.071	-	hexanal	wild type enzyme, at pH 8.0 and 25°C	Arabidopsis thaliana
0.072	-	hexanal	mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C	Arabidopsis thaliana
0.18	-	trans-2-hexenal	wild type enzyme, at pH 8.0 and 25°C	Arabidopsis thaliana
0.199	-	trans-2-hexenal	mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C	Arabidopsis thaliana
0.421	-	NAD+	wild type enzyme, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
0.441	-	NAD+	mutant enzyme E149D, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
0.496	-	NAD+	mutant enzyme I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
0.564	-	NAD+	mutant enzyme E149T/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
0.63	-	NAD+	mutant enzyme E149N, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
0.886	-	NAD+	mutant enzyme E149Q, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
0.991	-	NAD+	mutant enzyme E149D/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
0.992	-	NAD+	mutant enzyme E149N/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
1.068	-	NAD+	mutant enzyme E149T/V178R/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
1.092	-	NAD+	mutant enzyme E149T/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
1.262	-	NAD+	mutant enzyme E149T, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
1.622	-	NAD+	mutant enzyme E149T/V178R/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
1.648	-	NAD+	mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
1.649	-	NAD+	mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
1.829	-	NAD+	mutant enzyme E149T/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
1.856	-	NAD+	mutant enzyme E149Q/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
2.115	-	NAD+	mutant enzyme E149T/V178R/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
2.524	-	NAD+	mutant enzyme E149T/V178R/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
3.218	-	NAD+	mutant enzyme I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
3.418	-	NAD+	mutant enzyme E149T/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
3.651	-	NAD+	mutant enzyme E149D/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
4.059	-	NAD+	mutant enzyme E149Q/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
4.136	-	NAD+	mutant enzyme E149N/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q70DU8	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel affinity column chromatography, and Superdex 200 gel filtration	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
hexanal + NAD+ + H2O	-	Arabidopsis thaliana	hexanoate + NADH + H+	-	?
additional information	no activity with NADP+	Arabidopsis thaliana	?	-	?
nonanal + NAD+ + H2O	-	Arabidopsis thaliana	nonanoate + NADH + H+	-	?
trans-2-hexenal + NAD+ + H2O	-	Arabidopsis thaliana	(E)-hex-2-enoate + NADH + H+	-	?
trans-2-nonenal + NAD+ + H2O	-	Arabidopsis thaliana	(E)-non-2-enoate + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
ALDH3H1	-	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
1.1	-	trans-2-hexenal	mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C	Arabidopsis thaliana
1.8	-	NAD+	mutant enzyme E149T/V178R/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
2.2	-	trans-2-hexenal	wild type enzyme, at pH 8.0 and 25°C	Arabidopsis thaliana
2.2	-	NAD+	mutant enzyme E149T/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
2.7	-	trans-2-nonenal	wild type enzyme, at pH 8.0 and 25°C	Arabidopsis thaliana
3.1	-	NAD+	mutant enzyme E149T/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
3.8	-	trans-2-nonenal	mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C	Arabidopsis thaliana
4	-	NAD+	mutant enzyme E149T/V178R/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
8.2	-	hexanal	mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C	Arabidopsis thaliana
10.2	-	NAD+	mutant enzyme E149T, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
10.7	-	NAD+	mutant enzyme E149D, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
11.2	-	hexanal	wild type enzyme, at pH 8.0 and 25°C	Arabidopsis thaliana
11.4	-	NAD+	mutant enzyme E149Q/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
12.2	-	NAD+	mutant enzyme E149T/V178R/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
12.4	-	NAD+	mutant enzyme E149D/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
12.7	-	NAD+	mutant enzyme E149Q, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
12.7	-	NAD+	mutant enzyme E149T/V178R/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
13	-	Nonanal	mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C	Arabidopsis thaliana
13.1	-	NAD+	mutant enzyme E149N/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
15.7	-	NAD+	mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
16.3	-	NAD+	mutant enzyme I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
16.6	-	NAD+	mutant enzyme E149N, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
17.2	-	NAD+	wild type enzyme, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
17.9	-	Nonanal	wild type enzyme, at pH 8.0 and 25°C	Arabidopsis thaliana
18	-	NAD+	mutant enzyme E149T/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
18.5	-	NAD+	mutant enzyme I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
20.1	-	NAD+	mutant enzyme E149Q/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
23.2	-	NAD+	mutant enzyme E149D/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
26.9	-	NAD+	mutant enzyme E149N/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
33.9	-	NAD+	mutant enzyme E149T/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
1.1	-	NAD+	mutant enzyme E149T/V178R/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
2.8	-	NAD+	mutant enzyme E149T/I200V, with trans-2-hexenal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
3.8	-	NAD+	mutant enzyme E149T/V178R/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
4	-	NAD+	mutant enzyme E149T/I200V, with trans-2-nonenal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
4.8	-	NAD+	mutant enzyme E149T/V178R/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
5	-	NAD+	mutant enzyme E149Q/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
5.1	-	NAD+	mutant enzyme I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
5.3	-	trans-2-hexenal	mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C	Arabidopsis thaliana
6	-	NAD+	mutant enzyme E149T/V178R/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
6.1	-	NAD+	mutant enzyme E149Q/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
6.4	-	NAD+	mutant enzyme E149D/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
6.5	-	NAD+	mutant enzyme E149N/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
8.1	-	NAD+	mutant enzyme E149T, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
9.5	-	NAD+	mutant enzyme E149T/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
9.9	-	NAD+	mutant enzyme E149T/I200G, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
9.9	-	NAD+	mutant enzyme E149T/I200V, with nonanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
12.4	-	trans-2-hexenal	wild type enzyme, at pH 8.0 and 25°C	Arabidopsis thaliana
12.5	-	NAD+	mutant enzyme E149D/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
13.2	-	NAD+	mutant enzyme E149N/I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
14.3	-	NAD+	mutant enzyme E149Q, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
24.3	-	NAD+	mutant enzyme E149D, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
26.3	-	NAD+	mutant enzyme E149N, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
37.3	-	NAD+	mutant enzyme I200V, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
40.9	-	NAD+	wild type enzyme, with hexanal as cosubstrate, at pH 8.0 and 25°C	Arabidopsis thaliana
115	-	hexanal	mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C	Arabidopsis thaliana
120	-	trans-2-nonenal	mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C	Arabidopsis thaliana
154	-	hexanal	wild type enzyme, at pH 8.0 and 25°C	Arabidopsis thaliana
213	-	Nonanal	mutant enzyme E149T/V178R/I200V, at pH 8.0 and 25°C	Arabidopsis thaliana
918	-	trans-2-nonenal	wild type enzyme, at pH 8.0 and 25°C	Arabidopsis thaliana
2166	-	Nonanal	wild type enzyme, at pH 8.0 and 25°C	Arabidopsis thaliana