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Literature summary for 1.2.1.3 extracted from

  • Ho, K.K.; Allali-Hassani, A.; Hurley, T.D.; Weiner, H.
    Differential effects of Mg2+ ions on the individual kinetic steps of human cytosolic and mitochondrial aldehyde dehydrogenases (2005), Biochemistry, 44, 8022-8029.
    View publication on PubMed
Search for more literature for 1.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E399Q mutant is not inhibited by MgCl2 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ 85% inhibition at 0.5 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25°C Homo sapiens
0.0002
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C Homo sapiens
0.0003
-
 NAD+ 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,0.14 mM propanal, 25°C Homo sapiens
0.0012
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25°C Homo sapiens
0.0052
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C Homo sapiens
0.0062
-
 NAD+ 50 mM sodium phosphate buffer, pH 7.4, 0.14 mM propanal, 25°C Homo sapiens
0.041
-
 NAD+ 50 mM sodium phosphate buffer, pH 7.4, 0.014 mM propanal, 25°C Homo sapiens
0.12
-
 NAD+ 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,0.014 mM propanal, 25°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P05091
-
-

Purification (Commentary)

Purification (Comment) Organism
two chromatography steps, homogeneous on SDS-PAGE Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
 Homo sapiens acetate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O
-
 Homo sapiens benzoate + NADH + H+
-
 ?
propanal + NAD+ + H2O
-
 Homo sapiens propionate + NADH
-
 ?

Synonyms

Synonyms Comment Organism
ALDH1
-
 Homo sapiens
ALDH2
-
 Homo sapiens
class 1 aldehyde dehydrogenase
-
 Homo sapiens
class 2 aldehyde dehydrogenase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25°C Homo sapiens
0.65
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25°C Homo sapiens
1.33
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C Homo sapiens
3.16
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 1 mM NAD+, 25°C Homo sapiens
6.16
-
 propanal 50 mM sodium phosphate buffer, pH 7.4, 0.2 mM Mg2+,1 mM NAD+, 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens